##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660672_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30188876 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3933311395893 34.0 31.0 34.0 31.0 34.0 2 32.55133486917499 34.0 31.0 34.0 31.0 34.0 3 32.571498488383604 34.0 31.0 34.0 31.0 34.0 4 35.93255240771468 37.0 37.0 37.0 35.0 37.0 5 35.930170603238096 37.0 37.0 37.0 35.0 37.0 6 35.943644539796715 37.0 37.0 37.0 35.0 37.0 7 35.90975828315039 37.0 36.0 37.0 35.0 37.0 8 35.92028169581405 37.0 37.0 37.0 35.0 37.0 9 37.649242787310136 39.0 38.0 39.0 35.0 39.0 10-11 37.5979605368547 39.0 38.0 39.0 35.0 39.0 12-13 37.6365676714827 39.0 38.0 39.0 35.0 39.0 14-15 39.11600282832657 41.0 39.0 41.0 36.0 41.0 16-17 39.076067456105356 41.0 39.0 41.0 36.0 41.0 18-19 39.044175874583736 41.0 39.0 41.0 36.0 41.0 20-21 38.986520879412666 41.0 39.0 41.0 35.5 41.0 22-23 38.872922827600476 40.5 39.0 41.0 35.0 41.0 24-25 38.85095783294483 40.0 39.0 41.0 35.0 41.0 26-27 38.80348178580746 40.0 39.0 41.0 35.0 41.0 28-29 38.71777142348725 40.0 38.5 41.0 35.0 41.0 30-31 38.62134963222877 40.0 38.0 41.0 34.5 41.0 32-33 38.517988894319885 40.0 38.0 41.0 34.0 41.0 34-35 38.41980143613164 40.0 38.0 41.0 34.0 41.0 36-37 38.28016346153464 40.0 38.0 41.0 34.0 41.0 38-39 37.98897839058334 40.0 38.0 41.0 33.0 41.0 40-41 37.85417256011784 40.0 37.0 41.0 33.0 41.0 42-43 37.70531362280596 40.0 37.0 41.0 33.0 41.0 44-45 37.56974451450263 40.0 37.0 41.0 33.0 41.0 46-47 37.39846334457765 40.0 36.0 41.0 32.0 41.0 48-49 37.42797524160886 40.0 36.0 41.0 32.0 41.0 50-51 36.47285377567552 38.5 35.5 40.0 31.5 40.5 52-53 36.69367885707305 39.0 35.5 39.5 31.5 40.5 54-55 37.19999187780294 40.0 36.0 41.0 31.5 41.0 56-57 36.990709160553045 40.0 36.0 41.0 31.0 41.0 58-59 36.778496754897404 39.5 35.5 41.0 31.0 41.0 60-61 36.55178737028831 39.0 35.0 41.0 30.0 41.0 62-63 36.28039606376865 39.0 35.0 41.0 30.0 41.0 64-65 35.981983843982796 38.0 35.0 41.0 29.5 41.0 66-67 35.655358566513044 37.0 35.0 40.0 29.0 41.0 68-69 35.336289118548166 37.0 35.0 40.0 29.0 41.0 70-71 34.99345636452314 36.0 35.0 39.0 29.0 41.0 72-73 34.63710671109451 36.0 34.0 39.0 29.0 41.0 74-75 34.28040394746727 35.0 34.0 38.0 28.0 40.0 76-77 33.92393892041558 35.0 34.0 37.0 28.0 39.5 78-79 33.58507070617667 35.0 34.0 37.0 27.0 39.0 80-81 33.27047336906482 35.0 34.0 36.0 27.0 39.0 82-83 33.002482305071574 35.0 34.0 36.0 26.0 37.0 84-85 32.76682467409519 35.0 33.5 35.5 26.0 37.0 86-87 32.56528133077893 35.0 33.0 35.0 26.0 36.5 88-89 32.38062742051079 35.0 33.0 35.0 25.0 36.0 90-91 32.24878175325242 35.0 33.0 35.0 25.0 36.0 92-93 32.12189383930691 35.0 33.0 35.0 25.0 36.0 94-95 32.01701346880222 35.0 33.0 35.0 25.0 35.5 96-97 31.915137665277765 35.0 33.0 35.0 24.0 35.0 98-99 31.671001944557325 35.0 33.0 35.0 23.5 35.0 100 31.605802978554088 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5076757191743013 1101 2 0.48181144030352385 1101 3 0.49106701523688656 1101 4 0.43241730010748824 1101 5 0.4139915619312049 1101 6 0.4095658745831514 1101 7 0.41572842942574084 1101 8 0.4092832188777251 1101 9 0.5190782089454018 1101 10-11 0.49162713759756826 1101 12-13 0.5006163154767691 1101 14-15 0.6411826310823798 1101 16-17 0.6535583998505885 1101 18-19 0.6156787837664979 1101 20-21 0.6303470241075715 1101 22-23 0.6310663201233808 1101 24-25 0.6463066633768833 1101 26-27 0.6312926786995163 1101 28-29 0.6408954638616677 1101 30-31 0.6489227354137697 1101 32-33 0.6788529357999522 1101 34-35 0.6525993927783631 1101 36-37 0.640245242062953 1101 38-39 0.7073746180360061 1101 40-41 0.7096929479645198 1101 42-43 0.7211889176086999 1101 44-45 0.6838330198688283 1101 46-47 0.6646281309106286 1101 48-49 0.6761110737803975 1101 50-51 0.6331972574505329 1101 52-53 0.6948776078548633 1101 54-55 0.7679981211714804 1101 56-57 0.75122161496747 1101 58-59 0.6839283235368754 1101 60-61 0.6988762707923897 1101 62-63 0.6759581407025337 1101 64-65 0.653464304788379 1101 66-67 0.6491525981584303 1101 68-69 0.6003455090383767 1101 70-71 0.5796153137394455 1101 72-73 0.5526080962775524 1101 74-75 0.5716402955522568 1101 76-77 0.5402841615703267 1101 78-79 0.5611443955416462 1101 80-81 0.546633460543454 1101 82-83 0.5169288727737609 1101 84-85 0.5564894279512274 1101 86-87 0.5725582479486135 1101 88-89 0.550308822463137 1101 90-91 0.5386138020596505 1101 92-93 0.5659190559498555 1101 94-95 0.5555390742614179 1101 96-97 0.5150219790236825 1101 98-99 0.37139147645775594 1101 100 0.4977370704186477 1102 1 0.48627740015409415 1102 2 0.45112160650141675 1102 3 0.4633181361323082 1102 4 0.40666629784149677 1102 5 0.3904786963227167 1102 6 0.33970044837282387 1102 7 0.35633793328622687 1102 8 0.3922983229970711 1102 9 0.46303596664989755 1102 10-11 0.4760807821145434 1102 12-13 0.46722782316243183 1102 14-15 0.5384900169933999 1102 16-17 0.5853325802715617 1102 18-19 0.566619634465205 1102 20-21 0.5330830763676104 1102 22-23 0.5757137943818549 1102 24-25 0.5958853374172364 1102 26-27 0.5829942794747538 1102 28-29 0.5936778901158917 1102 30-31 0.5981375309238288 1102 32-33 0.6062764527105742 1102 34-35 0.5957839567664038 1102 36-37 0.6134194846115761 1102 38-39 0.6053339414697234 1102 40-41 0.6294325975988144 1102 42-43 0.665541340487863 1102 44-45 0.6044316520829227 1102 46-47 0.6453238414406641 1102 48-49 0.6165139659121905 1102 50-51 0.6008671887371193 1102 52-53 0.6637212336451199 1102 54-55 0.6942765118400018 1102 56-57 0.669709481360627 1102 58-59 0.6220600471897342 1102 60-61 0.5788385854487501 1102 62-63 0.5903522204865368 1102 64-65 0.6122265421699993 1102 66-67 0.54751877170321 1102 68-69 0.5269530657667332 1102 70-71 0.5578484595253386 1102 72-73 0.5550406255415155 1102 74-75 0.5846440193955829 1102 76-77 0.5612025772214793 1102 78-79 0.5092536917232451 1102 80-81 0.5359076416342745 1102 82-83 0.5277263590291454 1102 84-85 0.5431461104478075 1102 86-87 0.5344410449554928 1102 88-89 0.5279527506260777 1102 90-91 0.4881332782640868 1102 92-93 0.5146889294282104 1102 94-95 0.5186561097902 1102 96-97 0.5311332783467542 1102 98-99 0.3265554784672524 1102 100 0.3666018553860759 1103 1 0.39927942877467615 1103 2 0.40326322543464954 1103 3 0.3723591570449756 1103 4 0.3394136349378698 1103 5 0.36329562962382056 1103 6 0.2996268704997007 1103 7 0.3324497551127408 1103 8 0.36036874317037615 1103 9 0.40034077746940966 1103 10-11 0.42057385034475914 1103 12-13 0.3832609879444604 1103 14-15 0.48298689392201055 1103 16-17 0.5264702003284114 1103 18-19 0.5105964930757239 1103 20-21 0.5103007331178802 1103 22-23 0.508314329497594 1103 24-25 0.4997346221299992 1103 26-27 0.4842968800199188 1103 28-29 0.5162729585907044 1103 30-31 0.5134422418338502 1103 32-33 0.5387374186781386 1103 34-35 0.5203041669434825 1103 36-37 0.5157716611717902 1103 38-39 0.5466499330491601 1103 40-41 0.5770144194693003 1103 42-43 0.5866385199262041 1103 44-45 0.5932850873113225 1103 46-47 0.5624295612167955 1103 48-49 0.4884647306665002 1103 50-51 0.4281257234176934 1103 52-53 0.5186389643071436 1103 54-55 0.5607802425683914 1103 56-57 0.5513735698032534 1103 58-59 0.5248958839443034 1103 60-61 0.5269114103415262 1103 62-63 0.48643586388971016 1103 64-65 0.4817396719381293 1103 66-67 0.4629936573735307 1103 68-69 0.4503021809546226 1103 70-71 0.4397104650993029 1103 72-73 0.4109055197109299 1103 74-75 0.41593275397792695 1103 76-77 0.42198921739167616 1103 78-79 0.4087063379334239 1103 80-81 0.42313101994629676 1103 82-83 0.43415210057873566 1103 84-85 0.4477718658373462 1103 86-87 0.40195204458310485 1103 88-89 0.41310958846820256 1103 90-91 0.44425997712178855 1103 92-93 0.4464066475517612 1103 94-95 0.40994141624170766 1103 96-97 0.40975768036872395 1103 98-99 0.27451191386968077 1103 100 0.29004460275553257 1104 1 0.37105184228538235 1104 2 0.3542312097232383 1104 3 0.3422732959596999 1104 4 0.3173711626981799 1104 5 0.31817960771751075 1104 6 0.304844191129078 1104 7 0.3223971142172104 1104 8 0.31039491623334214 1104 9 0.38970242914828646 1104 10-11 0.40089600821978166 1104 12-13 0.37735312218402584 1104 14-15 0.47466652097864426 1104 16-17 0.45674600065773774 1104 18-19 0.4575786344158175 1104 20-21 0.4573440569277878 1104 22-23 0.4584804652387291 1104 24-25 0.4942210850357114 1104 26-27 0.4670888191530409 1104 28-29 0.4627604833903902 1104 30-31 0.4891317580608572 1104 32-33 0.4835929925213023 1104 34-35 0.4572552999693116 1104 36-37 0.4656681215131684 1104 38-39 0.4817540691134852 1104 40-41 0.44736787389886246 1104 42-43 0.4794123891254003 1104 44-45 0.42285488641805813 1104 46-47 0.45275535224353547 1104 48-49 0.4254171894811094 1104 50-51 0.3432201724262214 1104 52-53 0.454941524528806 1104 54-55 0.510087910151455 1104 56-57 0.49389153050137935 1104 58-59 0.48449679013273084 1104 60-61 0.4539363451698861 1104 62-63 0.45786111922605244 1104 64-65 0.42530039156512345 1104 66-67 0.3868924014251576 1104 68-69 0.43698771112792656 1104 70-71 0.431557233504023 1104 72-73 0.40286827854023244 1104 74-75 0.4101550538530532 1104 76-77 0.3856573431371686 1104 78-79 0.39150704106680223 1104 80-81 0.40130632345705664 1104 82-83 0.3840263735000775 1104 84-85 0.3844930304541023 1104 86-87 0.38926911232432104 1104 88-89 0.3728868812739847 1104 90-91 0.41897855155497865 1104 92-93 0.4246950561722045 1104 94-95 0.41502911304631596 1104 96-97 0.39454959716114857 1104 98-99 0.2710732844911483 1104 100 0.30063214347878287 1105 1 0.3104340157372292 1105 2 0.3027381021962583 1105 3 0.31245540441518926 1105 4 0.3016160571909836 1105 5 0.30997304203073384 1105 6 0.2799024369377676 1105 7 0.28364711588464786 1105 8 0.2785381834780978 1105 9 0.33694902374798374 1105 10-11 0.33382262861151446 1105 12-13 0.3541609615520045 1105 14-15 0.4252046123497095 1105 16-17 0.4448413126066768 1105 18-19 0.44258837064124634 1105 20-21 0.43540363650911473 1105 22-23 0.4486930926845858 1105 24-25 0.4269296776558136 1105 26-27 0.44462672124257807 1105 28-29 0.4297479572366427 1105 30-31 0.454518241352865 1105 32-33 0.4404498643925905 1105 34-35 0.45117848580498077 1105 36-37 0.46324459583528466 1105 38-39 0.48504467356077896 1105 40-41 0.4945713793619717 1105 42-43 0.4759863244415641 1105 44-45 0.4777376798530426 1105 46-47 0.44616173871870757 1105 48-49 0.4241315053715553 1105 50-51 0.38842336568334446 1105 52-53 0.4521926033534953 1105 54-55 0.48660328559434873 1105 56-57 0.4879664058485176 1105 58-59 0.46086752256388763 1105 60-61 0.4359000351399871 1105 62-63 0.4404734143807474 1105 64-65 0.44699805867249864 1105 66-67 0.44440242786870954 1105 68-69 0.41759264269318663 1105 70-71 0.41638058702070424 1105 72-73 0.4166867296410075 1105 74-75 0.43612143189676544 1105 76-77 0.42378741414373167 1105 78-79 0.400342469878467 1105 80-81 0.37679927271825164 1105 82-83 0.37990717254400863 1105 84-85 0.41987134215060706 1105 86-87 0.41699679995407024 1105 88-89 0.41241209504259757 1105 90-91 0.3743690865982998 1105 92-93 0.3895698770718141 1105 94-95 0.39233673831512306 1105 96-97 0.36223788098761744 1105 98-99 0.2367908276896351 1105 100 0.2609868810755316 1106 1 0.3490388808924578 1106 2 0.3275152784890736 1106 3 0.3442325814790763 1106 4 0.2910523419892783 1106 5 0.29308839025483735 1106 6 0.2757915675988798 1106 7 0.2882839727858979 1106 8 0.31105511259553253 1106 9 0.361110330035153 1106 10-11 0.3758879061185709 1106 12-13 0.36093285538314746 1106 14-15 0.40311137114802165 1106 16-17 0.44896472233103424 1106 18-19 0.43451483605706187 1106 20-21 0.45168190924270846 1106 22-23 0.45689950946827196 1106 24-25 0.4339166298043793 1106 26-27 0.42505847634647154 1106 28-29 0.4427891275269076 1106 30-31 0.42836150721531396 1106 32-33 0.44215084512465097 1106 34-35 0.43918575694004147 1106 36-37 0.45304596761550187 1106 38-39 0.4406045859660921 1106 40-41 0.4487863797142424 1106 42-43 0.4526890900657392 1106 44-45 0.4196051808439236 1106 46-47 0.459061258268747 1106 48-49 0.4735620093077202 1106 50-51 0.47337375783663305 1106 52-53 0.48214996515709174 1106 54-55 0.4823285599137179 1106 56-57 0.4861802111932505 1106 58-59 0.43717427933970043 1106 60-61 0.4647030331145956 1106 62-63 0.46326910086045814 1106 64-65 0.38696679195998485 1106 66-67 0.3960783341464733 1106 68-69 0.3939425705736994 1106 70-71 0.3826272377550737 1106 72-73 0.3523870284961319 1106 74-75 0.36782697614711424 1106 76-77 0.3684917591420387 1106 78-79 0.3560358997795561 1106 80-81 0.3629351258361382 1106 82-83 0.32658037476122104 1106 84-85 0.3826067372275759 1106 86-87 0.37352632767480287 1106 88-89 0.37740744444877805 1106 90-91 0.33874396427562914 1106 92-93 0.35030658432092565 1106 94-95 0.36286380553265474 1106 96-97 0.3767248149812339 1106 98-99 0.27419067230589533 1106 100 0.2836800372706634 1107 1 0.3023455743812917 1107 2 0.25559338277761157 1107 3 0.2543644063759345 1107 4 0.26390044880477603 1107 5 0.28396353746399683 1107 6 0.23995811487827723 1107 7 0.22988499942294993 1107 8 0.26287599125801364 1107 9 0.2990587612414686 1107 10-11 0.33959293241314725 1107 12-13 0.2975067925391457 1107 14-15 0.34627782357792114 1107 16-17 0.37066188906889863 1107 18-19 0.37697281935545135 1107 20-21 0.38093146907493036 1107 22-23 0.3823621191459665 1107 24-25 0.37033476706618984 1107 26-27 0.3499833524757179 1107 28-29 0.3407253667616317 1107 30-31 0.37541584332529254 1107 32-33 0.40447674398894407 1107 34-35 0.4055110109394846 1107 36-37 0.39017393602900796 1107 38-39 0.39428700350844537 1107 40-41 0.43705888951725314 1107 42-43 0.4164844617299792 1107 44-45 0.45795705469188164 1107 46-47 0.42507709584838693 1107 48-49 0.3788926088863036 1107 50-51 0.26192327639920876 1107 52-53 0.3444692545303596 1107 54-55 0.4389837844733009 1107 56-57 0.4025198582154914 1107 58-59 0.4329340719107009 1107 60-61 0.41327163284141477 1107 62-63 0.3958752523525675 1107 64-65 0.3617933387256258 1107 66-67 0.3802941997633411 1107 68-69 0.3736711520945377 1107 70-71 0.3408832393267929 1107 72-73 0.3502232045982723 1107 74-75 0.3416767638713196 1107 76-77 0.34331060872089836 1107 78-79 0.33060355191555146 1107 80-81 0.31471547257997656 1107 82-83 0.33361191995984996 1107 84-85 0.3849079803946651 1107 86-87 0.35956278588559343 1107 88-89 0.358290572224675 1107 90-91 0.34086892184895845 1107 92-93 0.35236619077689824 1107 94-95 0.38512907975555777 1107 96-97 0.3799383804872427 1107 98-99 0.24746194860167492 1107 100 0.28030757187113764 1108 1 0.11333805306122713 1108 2 0.11931194325590866 1108 3 0.11442017102535829 1108 4 0.13414751779842504 1108 5 0.14541010915565522 1108 6 0.13124497231035548 1108 7 0.125981039836077 1108 8 0.12596652953128995 1108 9 0.15943039413913596 1108 10-11 0.16625062512796518 1108 12-13 0.1340369428974384 1108 14-15 0.1335241022400666 1108 16-17 0.1960888860447696 1108 18-19 0.1641259159385413 1108 20-21 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-0.6760841596350424 2316 96-97 -0.6912655815013515 2316 98-99 -0.5302853205125757 2316 100 -0.5761310603133083 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 846.0 3 296.0 4 252.0 5 250.0 6 607.0 7 4420.0 8 9935.0 9 14280.0 10 17968.0 11 18392.0 12 19567.0 13 22802.0 14 28054.0 15 36964.0 16 49144.0 17 62441.0 18 69627.0 19 77220.0 20 89008.0 21 107525.0 22 138530.0 23 197263.0 24 280790.0 25 357090.0 26 374052.0 27 342768.0 28 311993.0 29 328421.0 30 381584.0 31 466779.0 32 585215.0 33 766852.0 34 1087568.0 35 1747416.0 36 3358302.0 37 7223631.0 38 9020846.0 39 2585166.0 40 5012.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.854023707331617 30.145725066572925 28.821338310002652 24.178912916092806 2 22.65700767276565 31.398263482272004 25.99967090442126 19.945057940541076 3 24.66110425743699 25.179582606607543 26.834380467837143 23.32493266811833 4 20.70231403525841 27.630374259082245 24.573708232758356 27.093603472900984 5 23.103698735992538 29.471728486860837 26.237607810597453 21.18696496654917 6 27.13484230938877 27.174610773123003 25.39012528093771 20.30042163655052 7 22.595949824160797 25.665239288758368 29.48372568136552 22.255085205715314 8 25.969826739858192 26.860261457224844 25.315582191650304 21.85432961126666 9 25.001733001409992 25.471117924417015 27.16859777587328 22.358551298299712 10-11 22.05712844743737 28.412285671161676 27.642428790885017 21.888157090515936 12-13 21.911197320521588 26.646790383185582 27.670102704107048 23.771909592185786 14-15 21.892099188220165 30.06604908525275 26.099751342359678 21.942100384167407 16-17 23.76903114721351 26.3771227028905 26.13118594126835 23.722660208627644 18-19 21.945724192523784 28.13102921860871 26.22159047472849 23.70165611413901 20-21 23.666513523317562 28.326888230905734 26.149528088159546 21.85707015761716 22-23 23.75687033773367 26.5387197580732 27.803846892162593 21.900563012030542 24-25 22.05330275361879 28.053146577354426 26.01084706109666 23.88270360793012 26-27 22.051591382079657 26.413430096328995 29.58023892315288 21.954739598438472 28-29 22.120475269686075 26.44935404715889 29.528671182016918 21.901499501138115 30-31 22.054926744407748 26.2922368635306 29.62528307268808 22.027553319373578 32-33 22.188386676743786 26.456121230330005 29.391852836844627 21.96363925608158 34-35 22.19215799572324 26.634575226452352 29.22487963018268 21.948387147641725 36-37 22.074323245559775 26.589221822023866 29.37132101362267 21.965133918793693 38-39 22.12847771603859 26.533611616600883 29.396595814536386 21.94131485282414 40-41 22.246188933185575 26.59482937639902 29.27674431391752 21.882237376497887 42-43 22.25916983020045 26.59941668937674 29.274115404718337 21.867298075704475 44-45 22.393072824047433 26.679982093024464 29.160157512866018 21.766787570062085 46-47 22.333007807064025 26.75337213583491 29.119834576018878 21.79378548108219 48-49 22.45170649052462 26.748241087377835 28.97611562495087 21.823936797146676 50-51 22.55822387631353 26.79348278777499 28.921835571311007 21.726457764600475 52-53 22.641585104593183 26.938215631862526 28.75540595081818 21.664793312726108 54-55 22.887708355546195 27.044859523372327 28.266869577093384 21.800562543988093 56-57 23.239488585860812 27.42009423298068 27.63594140450846 21.704475776650042 58-59 23.433148101268486 27.694987054982455 27.240030747781695 21.63183409596736 60-61 23.57818411890945 27.863895659351297 27.01712584251228 21.540794379226973 62-63 23.69664696602797 27.98151682978312 26.886640141210794 21.435196062978115 64-65 23.88197400659895 28.015336445925016 26.834908516259727 21.26778103121631 66-67 24.010544508303283 27.885112073710506 26.9143529975022 21.189990420484012 68-69 24.146118033686147 27.822697692917618 27.00090589004799 21.030278383348243 70-71 24.320706300030054 27.72762111344945 27.052946655705078 20.898725930815417 72-73 24.4727644398543 27.647501929094968 27.0470605059719 20.832673125078838 74-75 24.70822494984908 27.683462399360558 26.923532831848977 20.684779818941383 76-77 24.961138121815736 27.72507694996524 26.72690627835232 20.586878649866705 78-79 25.19867368128994 27.733083520677088 26.52595667588851 20.542286122144464 80-81 25.42941393059501 27.804313016573424 26.334175813266615 20.43209723956495 82-83 25.6048555284001 27.845977348990303 26.136475904715844 20.41269121789376 84-85 25.74823852942383 27.822904349892713 26.0372020173281 20.391655103355358 86-87 25.916411616115674 27.80134346450754 25.962026951934913 20.320217967441874 88-89 26.052539793262643 27.78997788240996 25.885579666311376 20.271902658016025 90-91 26.185959254075275 27.71176709134674 25.850842394219704 20.25143126035828 92-93 26.266420831047988 27.76381125066593 25.821517674591878 20.148250243694207 94-95 26.350086487308076 27.801225602602468 25.75903065159637 20.08965725849308 96-97 26.398583359212502 27.839935270937303 25.69533276366032 20.066148606189874 98-99 26.46145020056941 27.947674179047738 25.622502851036344 19.968372769346505 100 26.4628423332892 28.06058207660585 25.524902556353734 19.951673033751216 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 44989.0 1 29035.0 2 10521.5 3 7441.5 4 6765.0 5 6672.0 6 6752.5 7 6880.0 8 6795.5 9 6867.5 10 7444.0 11 10735.5 12 92794.5 13 108562.0 14 43496.5 15 44148.0 16 49385.5 17 54733.5 18 59839.0 19 65232.5 20 72876.0 21 84494.0 22 99788.5 23 117759.5 24 138963.0 25 164701.5 26 193324.0 27 226530.0 28 269161.5 29 313145.5 30 352789.0 31 393648.5 32 437861.0 33 481832.0 34 523816.0 35 577228.0 36 643351.0 37 695005.5 38 738412.5 39 781161.5 40 815071.0 41 851608.0 42 895990.0 43 934714.5 44 970697.0 45 1015872.0 46 1058463.5 47 1100326.0 48 1131007.5 49 1145609.5 50 1157278.0 51 1163708.5 52 1159330.5 53 1138190.5 54 1095393.0 55 1038630.0 56 971309.5 57 888470.5 58 790401.0 59 678846.5 60 558491.5 61 444113.5 62 344016.0 63 255548.0 64 183693.0 65 129711.5 66 90397.0 67 63237.0 68 44082.0 69 30982.5 70 22553.5 71 16901.0 72 12963.5 73 9607.5 74 7513.0 75 6214.5 76 4625.5 77 2824.5 78 1772.0 79 1291.0 80 936.5 81 715.5 82 592.5 83 464.0 84 372.0 85 342.0 86 276.5 87 227.0 88 206.5 89 166.5 90 139.5 91 132.5 92 103.5 93 66.0 94 51.5 95 46.0 96 42.0 97 37.5 98 30.0 99 18.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.134930495590495 2 0.0505848578131892 3 0.018390880137438705 4 0.07532907154277621 5 0.07852561320931592 6 0.08039716351148682 7 0.047809000904836606 8 0.045099393564702446 9 0.03319103367743801 10-11 0.02264907113467888 12-13 0.014886277978683275 14-15 0.0119182973224972 16-17 0.016895296134907443 18-19 0.01614998849245 20-21 0.02290413197231987 22-23 0.01941774844482451 24-25 0.016668391363759288 26-27 0.015270525474350222 28-29 0.01712882586287744 30-31 0.014876340543450509 32-33 0.012115389787947057 34-35 0.009755248920165164 36-37 0.007676668717311635 38-39 0.016573985729048012 40-41 0.02537855334527857 42-43 0.02550939624250999 44-45 0.024318560253783546 46-47 0.026513077201019343 48-49 0.023013443759880296 50-51 0.023233723574206604 52-53 0.01965624689041089 54-55 0.02455871493857539 56-57 0.031206859109295756 58-59 0.023805126100090643 60-61 0.023584846285764332 62-63 0.03084911144091618 64-65 0.03154804438562071 66-67 0.024142998898004683 68-69 0.024873400387612973 70-71 0.035662142571985786 72-73 0.04028139371601645 74-75 0.041276793478498504 76-77 0.042313599221117076 78-79 0.03664097994241323 80-81 0.03219729015416142 82-83 0.03502780295629423 84-85 0.03820612599157385 86-87 0.038176313685875554 88-89 0.037469099545143714 90-91 0.040539767032068366 92-93 0.033699499113514525 94-95 0.033689561678281764 96-97 0.03365478065496708 98-99 0.31321470862313655 100 0.1492039650631577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.0188876E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.07584376548812 #Duplication Level Percentage of deduplicated Percentage of total 1 75.81831212762324 34.17574392027758 2 11.302170749236842 10.189097658069395 3 4.292008478295297 5.803977108233676 4 2.2596969390517865 4.074309847282002 5 1.3979379675606312 3.1506616709803503 6 0.9537646027659047 2.579504653399726 7 0.6459572384232699 2.038194729084743 8 0.49117270627675874 1.7712019336002531 9 0.3918955332916946 1.5898519647944167 >10 2.163706828391909 17.7796203395281 >50 0.18828108049271028 5.934747367258248 >100 0.09393632471410401 7.001850658024486 >500 7.608596470608637E-4 0.2172232882748133 >1k 3.619271293563624E-4 0.29298286569335996 >5k 0.0 0.0 >10k+ 3.6637099425631074E-5 3.4010319954988613 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 914827 3.030344687228501 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 35662 0.11812960509029882 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.1047438798317632 1.3249913643687827E-5 0.0 0.0 3.3124784109219567E-6 2 0.10475712974540688 1.987487046553174E-5 0.0 0.0 3.3124784109219567E-6 3 0.1047604422238178 2.6499827287375654E-5 0.0 3.3124784109219567E-6 6.624956821843913E-6 4 0.1047604422238178 2.981230569829761E-5 0.0 6.624956821843913E-6 1.3249913643687827E-5 5 0.1047604422238178 3.974974093106348E-5 0.0 6.624956821843913E-6 1.3249913643687827E-5 6 0.10476706718063965 5.631213298567327E-5 0.0 6.624956821843913E-6 1.6562392054609783E-5 7 0.10476706718063965 6.293708980751717E-5 0.0 9.93743523276587E-6 2.3187348876453697E-5 8 0.10477369213746149 6.95620466293611E-5 0.0 9.93743523276587E-6 2.3187348876453697E-5 9 0.10477369213746149 7.949948186212696E-5 0.0 9.93743523276587E-6 2.6499827287375654E-5 10-11 0.10479191076872156 9.771811312219773E-5 0.0 9.93743523276587E-6 3.478102331468055E-5 12-13 0.10481012939998163 1.1096802676588555E-4 0.0 9.93743523276587E-6 4.306221934198544E-5 14-15 0.10483497298806355 1.3415537564233923E-4 0.0 9.93743523276587E-6 5.465589378021229E-5 16-17 0.10486147281535094 1.4243657166964415E-4 0.0 1.6562392054609783E-5 6.624956821843913E-5 18-19 0.10489459759946014 1.6396768134063685E-4 0.0 1.821863126007076E-5 7.453076424574403E-5 20-21 0.10494262853641852 1.8715503021709054E-4 0.0 2.981230569829761E-5 7.784324265666598E-5 22-23 0.10522584544055234 2.053736614771613E-4 0.0 3.312478410921957E-5 9.274939550581479E-5 24-25 0.1064878997151136 2.2027981432631014E-4 0.0 3.478102331468055E-5 1.0434306994404165E-4 26-27 0.11194023917949114 2.4677964161368577E-4 0.0 3.643726252014153E-5 1.308428972314173E-4 28-29 0.1278086670070128 2.7162322969560046E-4 0.0 3.80935017256025E-5 1.4243657166964415E-4 30-31 0.16414986765323758 2.832169041338273E-4 0.0 3.974974093106348E-5 1.5734272451879295E-4 32-33 0.23089796387252046 2.881856217502103E-4 0.0 3.974974093106348E-5 1.689363989570198E-4 34-35 0.3380814840539277 3.0474801380482E-4 0.0 4.306221934198544E-5 1.7390511657340276E-4 36-37 0.4919924809390055 3.1965416665396884E-4 0.0 4.306221934198544E-5 1.9046750862801253E-4 38-39 0.6969421451795688 3.295916018867347E-4 0.0 4.471845854744642E-5 2.0371742227170037E-4 40-41 0.9512510502212802 3.395290371195006E-4 0.0 4.9687176163829354E-5 2.0868613988808327E-4 42-43 1.2565605291167514 3.594039075850323E-4 0.0 5.134341536929033E-5 2.1199861829900523E-4 44-45 1.614240291688899 3.892162132833299E-4 0.0 5.299965457475131E-5 2.219360535317711E-4 46-47 2.0246663042373623 4.206847581870885E-4 0.0 5.299965457475131E-5 2.2856101035361503E-4 48-49 2.4887213422586516 4.405596286526203E-4 0.0 5.299965457475131E-5 2.3849844558638088E-4 50-51 3.0026954299325355 4.62090738323613E-4 0.0 5.299965457475131E-5 2.5506083764099063E-4 52-53 3.558499494979541 4.7368441276183983E-4 0.0 5.631213298567327E-5 2.616857944628346E-4 54-55 4.157052750158701 4.935592832273715E-4 0.0 6.293708980751717E-5 2.699669904901395E-4 56-57 4.798060384891441 5.217153497202082E-4 0.0 6.624956821843913E-5 2.8652938254474924E-4 58-59 5.482968296004131 5.465589378021229E-4 0.0 6.624956821843913E-5 2.997792961884371E-4 60-61 6.204007065383951 5.664338082676547E-4 0.0 7.287452504028305E-5 3.11372970626664E-4 62-63 6.956935064425718 5.863086787331864E-4 0.0 7.287452504028305E-5 3.2296664506489077E-4 64-65 7.7366427951805825 6.012148315823352E-4 0.0 7.287452504028305E-5 3.2959160188673474E-4 66-67 8.544958745731375 6.12808506020562E-4 0.0 7.784324265666598E-5 3.362165587085786E-4 68-69 9.383423218539173 6.260584196642498E-4 0.0 7.949948186212696E-5 3.444977547358835E-4 70-71 10.240606506847092 6.359958548970157E-4 0.0 7.949948186212696E-5 3.560914291741103E-4 72-73 11.117571916225035 6.459332901297815E-4 0.0 8.281196027304892E-5 3.6934134281779816E-4 74-75 12.011914587346677 6.707768782116963E-4 0.0 8.281196027304892E-5 3.7265382122872014E-4 76-77 12.923356272025497 6.90651748677228E-4 0.0 8.943691709489283E-5 3.7762253884510307E-4 78-79 13.853914269613748 7.221202935809866E-4 0.0 9.274939550581479E-5 3.7762253884510307E-4 80-81 14.796032816856115 7.535888384847452E-4 0.0 9.606187391673675E-5 3.8093501725602506E-4 82-83 15.75259542620931 7.867136225939647E-4 0.0 9.606187391673675E-5 3.908724524887909E-4 84-85 16.721594735756312 8.065884930594965E-4 0.0 9.606187391673675E-5 3.991536485160958E-4 86-87 17.698211089409227 8.248071243195673E-4 0.0 9.606187391673675E-5 4.090910837488617E-4 88 18.431328811314472 8.347445595523331E-4 0.0 9.606187391673675E-5 4.1074732295432267E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 123835 0.0 80.84545 8 GTGGTAT 126650 0.0 79.22795 6 GTATCAA 126605 0.0 78.92706 9 TGGTATC 127355 0.0 78.48776 7 AGTGGTA 128310 0.0 78.29589 5 AAGCAGT 134025 0.0 75.779915 1 GCAGTGG 138200 0.0 73.4127 3 CAGTGGT 140365 0.0 71.84485 4 AGCAGTG 145580 0.0 69.91194 2 CAACGCA 120955 0.0 43.757988 12-13 ATCAACG 123010 0.0 43.09765 10-11 ACGCAGA 124830 0.0 42.44562 14-15 AGAGTAC 125125 0.0 41.810806 18-19 TCAACGC 120315 0.0 41.555824 12-13 AACGCAG 123205 0.0 40.513184 14-15 GAGTACT 120170 0.0 39.964706 20-21 CGCAGAG 126475 0.0 39.38578 16-17 TATCAAC 127785 0.0 39.065815 10-11 AGTACTT 131005 0.0 38.92624 20-21 GTACTTT 126130 0.0 38.0398 22-23 >>END_MODULE