FastQCFastQC Report
Thu 26 May 2016
SRR1660672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30188876
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8700022.8818628424589243No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA346960.11492975094534821No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT306000.10136183937421188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1185550.079.466848
GTGGTAT1216800.077.626726
GTATCAA1214400.077.401049
AGTGGTA1224800.077.158045
TGGTATC1224700.076.991747
AAGCAGT1283250.074.344421
GCAGTGG1320450.071.974493
CAGTGGT1349200.070.28414
AGCAGTG1388350.068.776412
CAACGCA1151350.043.31899312-13
ATCAACG1170150.042.70524610-11
ACGCAGA1188950.041.99951614-15
AGAGTAC1198850.041.32709518-19
TCAACGC1144650.041.01778812-13
AACGCAG1171550.039.9607214-15
GAGTACT1141600.039.7544820-21
CGCAGAG1204000.038.89826216-17
AGTACTT1257150.038.43068320-21
TATCAAC1228050.038.3736410-11
GTACTTT1206650.037.50941522-23
Produced by FastQC (version 0.11.5)