##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660672_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30188876
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78206803724657	34.0	31.0	34.0	31.0	34.0
2	32.96606743490549	34.0	31.0	34.0	31.0	34.0
3	33.01735940748506	34.0	33.0	34.0	31.0	34.0
4	36.39709004071566	37.0	37.0	37.0	35.0	37.0
5	36.32230951559773	37.0	37.0	37.0	35.0	37.0
6	36.31243925742714	37.0	37.0	37.0	35.0	37.0
7	36.27034838925437	37.0	37.0	37.0	35.0	37.0
8	36.2825866057418	37.0	37.0	37.0	35.0	37.0
9	38.06017749716816	39.0	38.0	39.0	35.0	39.0
10-11	38.01405885399642	39.0	38.0	39.0	35.0	39.0
12-13	38.04473545156169	39.0	38.0	39.0	35.0	39.0
14-15	39.556841566410085	41.0	40.0	41.0	37.0	41.0
16-17	39.530632226916964	41.0	39.5	41.0	37.0	41.0
18-19	39.498483448009125	41.0	39.0	41.0	37.0	41.0
20-21	39.46754299497603	41.0	39.0	41.0	36.5	41.0
22-23	39.356498748081904	41.0	39.0	41.0	36.0	41.0
24-25	39.343764703263545	41.0	39.0	41.0	36.0	41.0
26-27	39.281987494334004	41.0	39.0	41.0	36.0	41.0
28-29	39.20673504041687	40.5	39.0	41.0	36.0	41.0
30-31	39.121392263825925	40.0	39.0	41.0	36.0	41.0
32-33	39.0309169013116	40.0	39.0	41.0	35.0	41.0
34-35	38.9351490761034	40.0	38.5	41.0	35.0	41.0
36-37	38.805094499046604	40.0	38.0	41.0	35.0	41.0
38-39	38.55324986263152	40.0	38.0	41.0	35.0	41.0
40-41	38.436855549043955	40.0	38.0	41.0	34.5	41.0
42-43	38.30486368555093	40.0	38.0	41.0	34.0	41.0
44-45	38.185630975462615	40.0	37.5	41.0	34.0	41.0
46-47	38.05240428626757	40.0	37.0	41.0	33.5	41.0
48-49	37.90163058737265	40.0	37.0	41.0	33.0	41.0
50-51	37.95536552934266	40.0	37.0	41.0	33.5	41.0
52-53	37.88692056637021	40.0	37.0	41.0	33.0	41.0
54-55	37.49645117625445	40.0	36.5	41.0	33.0	41.0
56-57	37.2529996313874	40.0	36.0	41.0	32.5	41.0
58-59	37.03889674461547	39.0	36.0	41.0	32.0	41.0
60-61	36.80842276141715	39.0	35.0	41.0	32.0	41.0
62-63	36.54715082800698	39.0	35.0	41.0	31.0	41.0
64-65	36.265492660276585	38.0	35.0	40.0	31.0	41.0
66-67	35.979290732785145	37.0	35.0	40.0	31.0	41.0
68-69	35.67277880766412	37.0	35.0	39.5	31.0	41.0
70-71	35.33985725404285	36.0	35.0	39.0	30.5	41.0
72-73	34.97602443032328	36.0	35.0	39.0	30.0	40.5
74-75	34.62128035174281	35.0	34.0	37.5	30.0	40.0
76-77	32.767679575085864	34.5	32.0	36.0	27.0	38.5
78-79	33.91323307300344	35.0	34.0	37.0	29.5	39.0
80-81	33.88255857886196	35.0	34.0	36.0	30.0	39.0
82-83	33.65821625820054	35.0	34.0	36.0	30.0	37.0
84-85	33.432749566429706	35.0	34.0	35.5	29.5	37.0
86-87	33.24327808693507	35.0	34.0	35.0	29.0	36.5
88-89	33.079148806335155	35.0	34.0	35.0	29.0	36.0
90-91	32.96537988032413	35.0	34.0	35.0	29.0	36.0
92-93	32.866803371546524	35.0	34.0	35.0	29.0	36.0
94-95	32.76646098052806	35.0	34.0	35.0	29.0	35.5
96-97	32.68947684570966	35.0	34.0	35.0	29.0	35.0
98-99	32.59406342256664	35.0	34.0	35.0	28.5	35.0
100	32.541960356523376	35.0	34.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.39062513527969145
1101	2	0.3545149434213286
1101	3	0.3334652322761258
1101	4	0.22895388196707245
1101	5	0.23976593328741558
1101	6	0.2489234333881285
1101	7	0.26167130924886095
1101	8	0.26462183709778486
1101	9	0.31235500674615935
1101	10-11	0.326085879690325
1101	12-13	0.32994080998011555
1101	14-15	0.4085793518491272
1101	16-17	0.43340025688392814
1101	18-19	0.4385278952536211
1101	20-21	0.43650675504803615
1101	22-23	0.4505516888189476
1101	24-25	0.4592038549184352
1101	26-27	0.4824964683948423
1101	28-29	0.48746082466669804
1101	30-31	0.5086535312200837
1101	32-33	0.5268809359401558
1101	34-35	0.5319134394263614
1101	36-37	0.5568718398502526
1101	38-39	0.539167513697663
1101	40-41	0.5286520217982016
1101	42-43	0.5467070566477688
1101	44-45	0.5632899077650393
1101	46-47	0.5415171840008881
1101	48-49	0.5639960649421099
1101	50-51	0.5099008865205263
1101	52-53	0.5368263243931253
1101	54-55	0.47020578691171977
1101	56-57	0.4683311765445026
1101	58-59	0.4656275854792966
1101	60-61	0.45043290936274616
1101	62-63	0.3795310381566779
1101	64-65	0.396727390549259
1101	66-67	0.3872187417919193
1101	68-69	0.37794343987989976
1101	70-71	0.36202722383514185
1101	72-73	0.3925711909737757
1101	74-75	0.3884458296995206
1101	76-77	0.39263828652414645
1101	78-79	0.46448997904804656
1101	80-81	0.4329542195022995
1101	82-83	0.4297267296020095
1101	84-85	0.40507990076587674
1101	86-87	0.40996648034681016
1101	88-89	0.4493338164369334
1101	90-91	0.41711491999608796
1101	92-93	0.3487441032165677
1101	94-95	0.350298126114744
1101	96-97	0.36062584920728824
1101	98-99	0.3969541331060853
1101	100	0.4121051347116662
1102	1	0.3175094299490411
1102	2	0.2522489878185681
1102	3	0.2816028758973559
1102	4	0.19868760128484553
1102	5	0.20994157737167285
1102	6	0.21042783767256878
1102	7	0.22237664114807387
1102	8	0.21995898938187963
1102	9	0.26736019989620274
1102	10-11	0.2935571275875617
1102	12-13	0.2995883967284243
1102	14-15	0.37583233065168997
1102	16-17	0.3860052781143892
1102	18-19	0.3878414942626094
1102	20-21	0.38924762704728977
1102	22-23	0.4084637099747539
1102	24-25	0.40246437795951095
1102	26-27	0.44385397711752006
1102	28-29	0.41798719848940635
1102	30-31	0.4235539825163883
1102	32-33	0.4131508196973641
1102	34-35	0.41080178886705454
1102	36-37	0.4223665262321319
1102	38-39	0.46235529337061365
1102	40-41	0.4872982611353578
1102	42-43	0.4468313082588722
1102	44-45	0.47712760771988627
1102	46-47	0.4729397318080757
1102	48-49	0.4668382087491807
1102	50-51	0.45303280341567387
1102	52-53	0.45483284052532014
1102	54-55	0.39673409997610776
1102	56-57	0.4104109960640443
1102	58-59	0.41747689673241695
1102	60-61	0.33674024322816365
1102	62-63	0.36452990314112554
1102	64-65	0.39038807667704134
1102	66-67	0.3460244321276491
1102	68-69	0.3519678940346225
1102	70-71	0.36690151161160856
1102	72-73	0.3860934596241563
1102	74-75	0.40154456333926447
1102	76-77	0.39761306676404473
1102	78-79	0.4613602714810696
1102	80-81	0.4114219844461573
1102	82-83	0.4201953532198459
1102	84-85	0.41810342374494525
1102	86-87	0.43797348736895714
1102	88-89	0.46412050193068666
1102	90-91	0.4274789438623401
1102	92-93	0.38071240737711776
1102	94-95	0.4361190797833743
1102	96-97	0.41455275679266634
1102	98-99	0.4096169688477147
1102	100	0.4312125988380089
1103	1	0.26946224122380613
1103	2	0.24779724410030468
1103	3	0.24584465910455577
1103	4	0.16677901799962314
1103	5	0.17268170230802582
1103	6	0.16834511933640783
1103	7	0.19864046497046672
1103	8	0.1491885871136489
1103	9	0.22089042190034291
1103	10-11	0.24071066988791046
1103	12-13	0.25702981780095513
1103	14-15	0.311744415668997
1103	16-17	0.2925387279081306
1103	18-19	0.298605693423994
1103	20-21	0.3117047729474507
1103	22-23	0.3249782250460953
1103	24-25	0.3266179992366176
1103	26-27	0.30886038893867607
1103	28-29	0.3462628894031994
1103	30-31	0.34687086651013743
1103	32-33	0.312362401160577
1103	34-35	0.3179355360452476
1103	36-37	0.3231685692622861
1103	38-39	0.3416104153432258
1103	40-41	0.33364881343684516
1103	42-43	0.33481855394897053
1103	44-45	0.32457483845130497
1103	46-47	0.3064162756157387
1103	48-49	0.3033052718383189
1103	50-51	0.33352538375633145
1103	52-53	0.3288770417431337
1103	54-55	0.2834280213503959
1103	56-57	0.2743128761139815
1103	58-59	0.28129658245122613
1103	60-61	0.20865746020122344
1103	62-63	0.1797075907689205
1103	64-65	0.20877961135248313
1103	66-67	0.1944905081526329
1103	68-69	0.20886186307708243
1103	70-71	0.1867333379965288
1103	72-73	0.2065269117358639
1103	74-75	0.19662142534989613
1103	76-77	0.1175701214057483
1103	78-79	0.2971926564616538
1103	80-81	0.3005503257479418
1103	82-83	0.2944858208791956
1103	84-85	0.3075289677913631
1103	86-87	0.3135857681730059
1103	88-89	0.31775687595288105
1103	90-91	0.2529859953508691
1103	92-93	0.24671295581289598
1103	94-95	0.2596207205791998
1103	96-97	0.2831802785383033
1103	98-99	0.26703480688968284
1103	100	0.2783283602944806
1104	1	0.2175288892785474
1104	2	0.20928224634543824
1104	3	0.192120977322233
1104	4	0.15578446165461912
1104	5	0.1560064292030532
1104	6	0.14840876602606556
1104	7	0.16179339686084404
1104	8	0.1578376928327927
1104	9	0.20581602264987708
1104	10-11	0.2089045886686023
1104	12-13	0.20446229127091442
1104	14-15	0.21708753648699997
1104	16-17	0.2558385817182298
1104	18-19	0.24257154038167528
1104	20-21	0.285146308546949
1104	22-23	0.27727274990262174
1104	24-25	0.2926785851658522
1104	26-27	0.2634853937368078
1104	28-29	0.2789749024786161
1104	30-31	0.25535979671335696
1104	32-33	0.2766715036302685
1104	34-35	0.295331576165232
1104	36-37	0.31971504966644915
1104	38-39	0.2810021530778499
1104	40-41	0.3211721869397124
1104	42-43	0.28015273524147233
1104	44-45	0.29503400070226604
1104	46-47	0.2890730486671842
1104	48-49	0.3132835037966899
1104	50-51	0.26697498064204694
1104	52-53	0.28343794580343484
1104	54-55	0.21155882035434104
1104	56-57	0.21817412676704606
1104	58-59	0.24327680435570898
1104	60-61	0.1759312171973022
1104	62-63	0.1594927934604513
1104	64-65	0.200927389753204
1104	66-67	0.17265893518784736
1104	68-69	0.11101836014069733
1104	70-71	0.14872449344348837
1104	72-73	0.18295429838878619
1104	74-75	0.19531551574129935
1104	76-77	0.14903298886389393
1104	78-79	0.25594975250468366
1104	80-81	0.2341954235999495
1104	82-83	0.24985961585138483
1104	84-85	0.24044243025645784
1104	86-87	0.2803518522914672
1104	88-89	0.2931315932142482
1104	90-91	0.26973892749138173
1104	92-93	0.2765039285210946
1104	94-95	0.29225248772161905
1104	96-97	0.311204824691373
1104	98-99	0.2879018347999107
1104	100	0.2835555683959967
1105	1	0.17208014783111736
1105	2	0.13706139798136263
1105	3	0.12161084467607708
1105	4	0.1140841357424236
1105	5	0.09371339990030947
1105	6	0.11449632929784315
1105	7	0.11902679321304532
1105	8	0.11947084687694343
1105	9	0.15000324613301785
1105	10-11	0.15151851085310852
1105	12-13	0.1454775838274287
1105	14-15	0.20354496840906222
1105	16-17	0.19287511354682607
1105	18-19	0.20871806401907378
1105	20-21	0.21874216292566473
1105	22-23	0.20504551154932926
1105	24-25	0.21907264053439235
1105	26-27	0.18926153526431477
1105	28-29	0.19733064234157638
1105	30-31	0.20890757649413416
1105	32-33	0.2157902719451954
1105	34-35	0.21837298509814929
1105	36-37	0.20368555638970776
1105	38-39	0.18229741498519303
1105	40-41	0.19106046626311723
1105	42-43	0.18134644732018046
1105	44-45	0.24483208960111824
1105	46-47	0.23851309863532322
1105	48-49	0.24075505060284996
1105	50-51	0.2051642123859878
1105	52-53	0.2202263710739416
1105	54-55	0.09697707789041488
1105	56-57	0.07890581107655237
1105	58-59	0.017481560255774298
1105	60-61	0.04630229198508573
1105	62-63	-0.009849869388304455
1105	64-65	0.02939348455554125
1105	66-67	0.02056742369389042
1105	68-69	0.002852102664967049
1105	70-71	0.0436113231414339
1105	72-73	0.08094322122422426
1105	74-75	0.08377679511919922
1105	76-77	0.04099961511742123
1105	78-79	0.16494678420643538
1105	80-81	0.16969408825615773
1105	82-83	0.1750426904726865
1105	84-85	0.1900778922204367
1105	86-87	0.20258392468310404
1105	88-89	0.18942882936942596
1105	90-91	0.19034140612124872
1105	92-93	0.14594420674001185
1105	94-95	0.15466722849340186
1105	96-97	0.18601148199437034
1105	98-99	0.2049189287822344
1105	100	0.21851152140985874
1106	1	0.20436421000589178
1106	2	0.1894141311966422
1106	3	0.18550934642048844
1106	4	0.1262379909902478
1106	5	0.1381207809905618
1106	6	0.14229841617336092
1106	7	0.1361061854423653
1106	8	0.16717107094282113
1106	9	0.20735461969083246
1106	10-11	0.19085046497346525
1106	12-13	0.1830940339798275
1106	14-15	0.2530682154368833
1106	16-17	0.25340494770834
1106	18-19	0.26837471357945475
1106	20-21	0.2521275478617824
1106	22-23	0.2559308258078232
1106	24-25	0.25687587577872506
1106	26-27	0.2438114126995501
1106	28-29	0.272800293245929
1106	30-31	0.24116404575495665
1106	32-33	0.2665015571349443
1106	34-35	0.26937088692582023
1106	36-37	0.25423579433633847
1106	38-39	0.2531645302343435
1106	40-41	0.2443888504714593
1106	42-43	0.2546337030429342
1106	44-45	0.2704526786964081
1106	46-47	0.2788273819832128
1106	48-49	0.25247934682442263
1106	50-51	0.2437056147104144
1106	52-53	0.22591295887044538
1106	54-55	0.1488876819258067
1106	56-57	0.168241427625496
1106	58-59	0.1798421103452128
1106	60-61	0.1457707694311452
1106	62-63	0.11255816119110307
1106	64-65	0.1422498748215304
1106	66-67	0.1041818386009723
1106	68-69	0.1529822671759078
1106	70-71	0.10188967891515688
1106	72-73	0.10987064723850182
1106	74-75	0.10554791714886136
1106	76-77	0.08906370930058216
1106	78-79	0.24877303012586083
1106	80-81	0.2553424866001137
1106	82-83	0.2621204386423628
1106	84-85	0.2683309852162594
1106	86-87	0.25885797733594273
1106	88-89	0.27504722793411673
1106	90-91	0.2748440944605761
1106	92-93	0.22584539259740666
1106	94-95	0.24277625419814086
1106	96-97	0.21963376564482928
1106	98-99	0.23855978776977338
1106	100	0.23758996812372857
1107	1	0.17216128414809617
1107	2	0.1309406293297357
1107	3	0.14488023242613224
1107	4	0.10314877063006378
1107	5	0.10310972699275567
1107	6	0.10647200548122271
1107	7	0.1353723889397358
1107	8	0.12171694428732138
1107	9	0.17002313680227132
1107	10-11	0.1684641046442934
1107	12-13	0.1327658139258503
1107	14-15	0.18342203978637883
1107	16-17	0.15705037968104563
1107	18-19	0.18773988755013704
1107	20-21	0.1791755542118878
1107	22-23	0.19197670684951618
1107	24-25	0.18577189830499208
1107	26-27	0.17395368813210865
1107	28-29	0.196178364998282
1107	30-31	0.17196879037681612
1107	32-33	0.17738239859302496
1107	34-35	0.18017830707533022
1107	36-37	0.18384471699146587
1107	38-39	0.11976426498341652
1107	40-41	0.17608261979619755
1107	42-43	0.14099700739568988
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2315	100	-0.41440629475412294
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2316	3	-0.6234430070863155
2316	4	-0.3623796961243926
2316	5	-0.33174024320662454
2316	6	-0.32073799610556364
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2316	9	-0.42048554381675274
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2316	12-13	-0.39858069193026324
2316	14-15	-0.5409078960270008
2316	16-17	-0.5578251432247257
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2316	36-37	-0.5679719083437504
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2316	40-41	-0.6266284164661258
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2316	54-55	-0.4819188014245199
2316	56-57	-0.48468387354625264
2316	58-59	-0.47202218002856
2316	60-61	-0.4266687621220697
2316	62-63	-0.4028021904301369
2316	64-65	-0.3955751469143891
2316	66-67	-0.4253078247459072
2316	68-69	-0.4099857239651712
2316	70-71	-0.4273477942862911
2316	72-73	-0.43202512470617904
2316	74-75	-0.4559029650851585
2316	76-77	-0.531599971514602
2316	78-79	-0.665129953650677
2316	80-81	-0.6425554846158761
2316	82-83	-0.6375207471135766
2316	84-85	-0.6101944953460787
2316	86-87	-0.6334504784641055
2316	88-89	-0.7028625852077184
2316	90-91	-0.5980197611063716
2316	92-93	-0.5494598381235534
2316	94-95	-0.521419464533551
2316	96-97	-0.5708667033567707
2316	98-99	-1.3850372212202942
2316	100	-0.9411142508405383
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	17.0
7	315.0
8	515.0
9	194.0
10	60.0
11	72.0
12	115.0
13	402.0
14	1782.0
15	5571.0
16	12333.0
17	19277.0
18	24206.0
19	28533.0
20	37643.0
21	53071.0
22	80977.0
23	134119.0
24	225646.0
25	309516.0
26	327403.0
27	293070.0
28	283506.0
29	317414.0
30	376675.0
31	448119.0
32	554050.0
33	717608.0
34	1010817.0
35	1661604.0
36	3336033.0
37	7721204.0
38	9816497.0
39	2387819.0
40	2693.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	16.833921737015686	29.958045191498524	28.832134142908185	24.375898928577612
2	22.627254845093677	31.31898201096303	26.31593410882766	19.73782903511563
3	24.332562100026514	25.504540149159578	26.904993084207575	23.257904666606336
4	20.80761138639279	27.908985415687553	24.724574045088662	26.558829152831
5	23.148546504348158	29.410041632553657	26.2715577751222	21.16985408797598
6	26.877390201609362	27.35204848302401	25.4531404216573	20.317420893709325
7	22.580105334163484	25.889966887140815	29.27766174533957	22.25226603335613
8	25.651567815906763	27.001750578590606	25.438204456502454	21.90847714900018
9	24.709247207481326	25.582383391816244	27.323425357075237	22.384944043627193
10-11	22.033137305277613	28.447412218990863	27.619617239144645	21.89983323658688
12-13	21.878119940603288	26.796800914350037	27.609933208510313	23.71514593653636
14-15	21.826330996887727	30.049464246366774	26.067830415415266	22.056374341330233
16-17	23.589609629719238	26.562540784890437	26.1501521288835	23.697697456506827
18-19	21.9268284167411	28.1534944288603	26.27754018411334	23.642136970285268
20-21	23.526699272220856	28.283402224189473	26.222602694518777	21.967295809070894
22-23	23.544697415279046	26.737138769701286	27.75450503957847	21.963658775441196
24-25	22.034504381285274	28.06654856480735	26.038538350365688	23.860408703541697
26-27	22.063656179023262	26.41336488970387	29.443494655582537	22.079484275690326
28-29	22.08663434874256	26.568612525969705	29.342975232551925	22.001777892735817
30-31	22.041642456735303	26.425922098788913	29.442229848926882	22.090205595548905
32-33	22.139867868042955	26.635793031915934	29.147222813914436	22.077116286126675
34-35	22.148324912664165	26.765739208759108	29.032104770869626	22.053831107707104
36-37	22.085687527897228	26.71218598594365	29.111959997179447	22.090166488979676
38-39	22.206570847521775	26.669139104599132	29.143157303571048	21.981132744308045
40-41	22.16171702338651	26.852133951397118	29.110251604473987	21.875897420742383
42-43	22.177038904219533	26.756500014793716	29.08316349829942	21.983297582687335
44-45	22.23808114496184	26.849780491303193	29.005444231778032	21.90669413195693
46-47	22.315913917191594	26.95954268093897	28.91155965620098	21.812983745668454
48-49	22.314793812391382	27.026138912801102	28.78028395694904	21.878783317858474
50-51	22.408299511180534	27.06835511111887	28.608591482254308	21.91475389544629
52-53	22.483917162671666	27.184282764719814	28.394060347902993	21.937739724705526
54-55	22.57106372838354	27.339864428409967	27.99621215851883	22.092859684687667
56-57	22.8180444168368	27.673278547524426	27.404997074443045	22.10367996119573
58-59	22.921507906281317	27.944850304853443	26.963842542710946	22.16979924615429
60-61	22.93774422100915	28.080036512289503	26.7416064037357	22.240612862965648
62-63	22.855837836913054	28.193725346025982	26.733283042309843	22.21715377475112
64-65	22.910100676200056	28.34887060654603	26.505059599933862	22.235969117320053
66-67	22.775426020323632	28.334141447501302	26.53579002029532	22.354642511879742
68-69	22.694847716697307	28.326836620723782	26.56714921211941	22.411166450459497
70-71	22.634402649890404	28.31635955882951	26.556192258870432	22.493045532409653
72-73	22.548764189882846	28.304536960235232	26.49750155673795	22.649197293143967
74-75	22.549027484712884	28.387326343951315	26.35322981096365	22.71041636037215
76-77	22.545327806051333	28.46427432856765	26.184611731254194	22.80578613412682
78-79	22.533830938182646	28.54412352187014	25.970835150802845	22.951210389144364
80-81	22.527661604358144	28.68197726516084	25.732770400650608	23.05759072983041
82-83	22.48429217486393	28.752156581260692	25.533155283241705	23.230395960633675
84-85	22.426890796275938	28.786074446219388	25.403229706724446	23.383805050780225
86-87	22.3950210382259	28.79776564374997	25.309551204168546	23.49766211385559
88-89	22.344868677766215	28.815249450266467	25.219946414934384	23.619935457032938
90-91	22.31748838869753	28.751325177569697	25.159133522065463	23.772052911667313
92-93	22.28418045996832	28.74730867035682	25.136519901640575	23.83199096803428
94-95	22.251521501764955	28.728281379261734	25.088488023790433	23.931709095182878
96-97	22.188283839624074	28.68846212751606	25.085840953486528	24.037413079373334
98-99	22.170495233104358	28.674469771677273	25.069804935072764	24.085230060145598
100	22.070381319293556	28.70360172044005	25.043918471390413	24.18209848887598
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	45327.0
1	29246.0
2	10426.5
3	7228.5
4	6579.0
5	6314.0
6	6397.0
7	6452.5
8	6282.5
9	6478.5
10	6965.5
11	10062.5
12	87488.5
13	101731.5
14	39667.5
15	40350.0
16	45668.5
17	51262.0
18	56967.0
19	62987.5
20	71219.0
21	83943.5
22	100709.5
23	119690.5
24	141243.0
25	168062.0
26	198414.0
27	233043.5
28	277057.0
29	323061.5
30	363924.0
31	404734.0
32	449010.0
33	491626.0
34	531737.0
35	582254.0
36	647173.0
37	698232.5
38	739515.0
39	783388.0
40	819792.0
41	858195.5
42	904476.5
43	945045.5
44	982708.5
45	1027282.5
46	1063616.0
47	1091591.0
48	1114023.0
49	1133584.5
50	1156352.5
51	1172527.5
52	1172901.0
53	1151599.0
54	1105136.5
55	1043168.0
56	968377.5
57	879269.0
58	777271.5
59	663215.0
60	542525.0
61	430681.0
62	333586.0
63	246637.0
64	175880.5
65	123504.5
66	85547.5
67	59149.5
68	40394.0
69	27890.5
70	20191.5
71	14903.5
72	11228.0
73	8063.5
74	6053.5
75	4967.0
76	3584.0
77	1971.0
78	1052.5
79	647.5
80	371.0
81	227.5
82	146.5
83	94.0
84	65.0
85	43.5
86	27.5
87	16.0
88	11.0
89	8.0
90	6.5
91	3.5
92	3.0
93	3.0
94	2.5
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10646968108385353
2	0.07361983268274049
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	2.6499827287375654E-4
20-21	0.0022044543824685623
22-23	7.949948186212697E-4
24-25	8.148696890868013E-4
26-27	0.0067905807423900115
28-29	0.006676300237213204
30-31	0.007951604425418158
32-33	0.007903573488459789
34-35	0.007423264118876105
36-37	0.010946084908891606
38-39	0.01356128661431449
40-41	0.017065888773069922
42-43	0.023641158418750006
44-45	0.012009390478797554
46-47	0.018748627805818277
48-49	0.016461361463076665
50-51	0.019043438384390328
52-53	0.02142676660104868
54-55	0.11077921549646301
56-57	0.10391410398982726
58-59	0.10086496761257359
60-61	0.14379303157891668
62-63	0.09415388635204569
64-65	0.07308652365858205
66-67	0.08290305342934927
68-69	0.07611578516537018
70-71	0.053017873206011375
72-73	0.031745136851070575
74-75	0.020499272645990527
76-77	0.01264373009448911
78-79	0.008860879749216235
80-81	0.005717337737251298
82-83	5.979023531714132E-4
84-85	0.002477733851369624
86-87	0.0016363643349954465
88-89	0.0020338617443060817
90-91	0.003115385945472101
92-93	0.004728562931591093
94-95	0.0020206118306623938
96-97	0.0011345238557407703
98-99	0.0015436149394896317
100	0.0018318005612398421
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.0188876E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.66185866677708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.77441325747412	33.84226135463268
2	11.134589779520642	9.94582950090983
3	4.382335153475998	5.871696997649814
4	2.2584671226237267	4.034693577367342
5	1.4393609242136587	3.2142267083856026
6	0.9397425999762642	2.5182390709973723
7	0.6757384272996976	2.1125813895038514
8	0.5057482170061093	1.8070124311121087
9	0.36201476324704013	1.4551426972283465
>10	2.236525257885024	18.190379607676608
>50	0.19133082673362903	5.955470461418609
>100	0.0984794917213755	7.272912044946181
>500	8.587909620012822E-4	0.24437784984764158
>1k	3.4365529657052655E-4	0.25978123991964147
>5k	7.3903662993961525E-6	0.017873154862878438
>10k+	4.4342197609414356E-5	3.257521913541423
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	870002	2.8818628424589243	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34696	0.11492975094534821	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30600	0.10136183937421188	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.10444906925319114	1.987487046553174E-5	0.0	0.0	0.0
2	0.10446894412365668	3.312478410921957E-5	0.0	0.0	0.0
3	0.10446894412365668	3.643726252014153E-5	0.0	0.0	6.624956821843913E-6
4	0.10446894412365668	4.306221934198544E-5	0.0	0.0	9.93743523276587E-6
5	0.10447225660206759	5.962461139659522E-5	0.0	0.0	1.987487046553174E-5
6	0.10447556908047852	6.624956821843913E-5	0.0	0.0	2.3187348876453697E-5
7	0.10447556908047852	7.287452504028305E-5	0.0	3.3124784109219567E-6	2.3187348876453697E-5
8	0.10447556908047852	8.281196027304892E-5	0.0	3.3124784109219567E-6	2.3187348876453697E-5
9	0.10447888155888944	8.943691709489283E-5	0.0	3.3124784109219567E-6	2.6499827287375654E-5
10-11	0.1045020689077659	1.1262426597134653E-4	0.0	6.624956821843913E-6	2.8156066492836634E-5
12-13	0.10451366258220413	1.3415537564233923E-4	0.0	1.3249913643687827E-5	3.312478410921957E-5
14-15	0.10453519369187511	1.4243657166964415E-4	0.0	1.4906152849148805E-5	4.306221934198544E-5
16-17	0.10457163095439526	1.457490500805661E-4	0.0	1.6562392054609783E-5	4.6374697752907394E-5
18-19	0.10460475573850447	1.6065520292971491E-4	0.0	2.3187348876453697E-5	4.8030936958368374E-5
20-21	0.1046544429146683	1.7887383418978568E-4	0.0	2.4843588081914674E-5	6.12808506020562E-5
22-23	0.10494262853641852	1.9046750862801253E-4	0.0	2.6499827287375654E-5	6.956204662936108E-5
24-25	0.10620302657177431	2.1034237909354426E-4	0.0	2.6499827287375654E-5	7.6187003451205E-5
26-27	0.11168020962423378	2.2524853194269307E-4	0.0	2.981230569829761E-5	7.6187003451205E-5
28-29	0.1274095133584967	2.3518596717545892E-4	0.0	3.146854490375859E-5	7.6187003451205E-5
30-31	0.163537059147217	2.4015468479184185E-4	0.0	3.80935017256025E-5	7.784324265666598E-5
32-33	0.22982472086738176	2.550608376409907E-4	0.0	3.974974093106348E-5	8.612443868397088E-5
34-35	0.3363854951075356	2.6499827287375654E-4	0.0	3.974974093106348E-5	9.606187391673675E-5
36-37	0.4895230282836631	2.7162322969560046E-4	0.0	4.140598013652446E-5	9.771811312219773E-5
38-39	0.6931857946615834	2.832169041338273E-4	0.0	4.9687176163829354E-5	1.0103059153311969E-4
40-41	0.9458748977603538	2.898418609556712E-4	0.0	5.465589378021229E-5	1.0268683073858067E-4
42-43	1.2486238308441826	3.11372970626664E-4	0.0	5.631213298567327E-5	1.0599930914950261E-4
44-45	1.6035608612920864	3.2959160188673474E-4	0.0	5.962461139659522E-5	1.1759298358772946E-4
46-47	2.0103630224590012	3.4615399394134446E-4	0.0	6.293708980751717E-5	1.2421794040957338E-4
48-49	2.4701681506790782	3.7596629963964213E-4	0.0	6.790580742390011E-5	1.391240932587222E-4
50-51	2.978754492217597	3.8093501725602506E-4	0.0	6.95620466293611E-5	1.4740528928602707E-4
52-53	3.5292387169366624	3.9418493089971284E-4	0.0	7.453076424574403E-5	1.5071776769694903E-4
54-55	4.121854354564244	4.107473229543226E-4	0.0	8.281196027304892E-5	1.54030246107871E-4
56-57	4.756516936900864	4.3227843262531537E-4	0.0	8.612443868397088E-5	1.6231144213517588E-4
58-59	5.434493486938699	4.60434499118152E-4	0.0	8.612443868397088E-5	1.6728015975155884E-4
60-61	6.1491872039223985	4.7368441276183983E-4	0.0	8.612443868397088E-5	1.755613557788637E-4
62-63	6.895556495710538	4.836218479946057E-4	0.0	8.943691709489283E-5	1.8218631260070761E-4
64-65	7.66869392553734	5.034967184601375E-4	0.0	9.274939550581479E-5	1.8881126942255153E-4
66-67	8.470565449339684	5.200591105147472E-4	0.0	9.606187391673675E-5	1.921237478334735E-4
68-69	9.30255071437572	5.283403065420521E-4	0.0	9.606187391673675E-5	1.9874870465531742E-4
70-71	10.153932196746908	5.349652633638961E-4	0.0	9.937435232765871E-5	2.070299006826223E-4
72-73	11.024890426526646	5.498714162130449E-4	0.0	1.0765554835496361E-4	2.153110967099272E-4
74-75	11.914105381068179	5.780274827058814E-4	0.0	1.1924922279319044E-4	2.1862357512084915E-4
76-77	12.821128219546829	6.012148315823352E-4	0.0	1.1924922279319044E-4	2.2690477114815406E-4
78-79	13.74707027846946	6.177772236369449E-4	0.0	1.1924922279319044E-4	2.3352972796999796E-4
80-81	14.685624598941676	6.326833764860937E-4	0.0	1.225617012041124E-4	2.3849844558638088E-4
82-83	15.637034648126681	6.409645725133986E-4	0.0	1.308428972314173E-4	2.4512340240822483E-4
84-85	16.60159854908146	6.740893566226183E-4	0.0	1.3249913643687827E-4	2.534045984355297E-4
86-87	17.574788143818274	6.856830310608451E-4	0.0	1.3249913643687827E-4	2.583733160519126E-4
88	18.307167183037883	6.88995509471767E-4	0.0	1.3249913643687827E-4	2.583733160519126E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	118555	0.0	79.46684	8
GTGGTAT	121680	0.0	77.62672	6
GTATCAA	121440	0.0	77.40104	9
AGTGGTA	122480	0.0	77.15804	5
TGGTATC	122470	0.0	76.99174	7
AAGCAGT	128325	0.0	74.34442	1
GCAGTGG	132045	0.0	71.97449	3
CAGTGGT	134920	0.0	70.2841	4
AGCAGTG	138835	0.0	68.77641	2
CAACGCA	115135	0.0	43.318993	12-13
ATCAACG	117015	0.0	42.705246	10-11
ACGCAGA	118895	0.0	41.999516	14-15
AGAGTAC	119885	0.0	41.327095	18-19
TCAACGC	114465	0.0	41.017788	12-13
AACGCAG	117155	0.0	39.96072	14-15
GAGTACT	114160	0.0	39.75448	20-21
CGCAGAG	120400	0.0	38.898262	16-17
AGTACTT	125715	0.0	38.430683	20-21
TATCAAC	122805	0.0	38.37364	10-11
GTACTTT	120665	0.0	37.509415	22-23
>>END_MODULE