FastQCFastQC Report
Thu 26 May 2016
SRR1660671_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660671_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48957781
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12446442.542280255716655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710130.1450494661921054No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT521410.10650196748091993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1731950.080.211158
GTGGTAT1770650.078.6317146
GTATCAA1768450.078.3890469
TGGTATC1779750.077.9873057
AGTGGTA1790650.077.849655
AAGCAGT1909150.073.8103261
GCAGTGG1948150.072.2685243
CAGTGGT1963200.071.316954
AGCAGTG2064850.068.454042
CAACGCA1677650.043.56842412-13
ATCAACG1704800.042.88784810-11
ACGCAGA1747200.041.86303714-15
TCAACGC1670550.041.50080512-13
AGAGTAC1755700.040.99934418-19
AACGCAG1709250.040.48203714-15
GAGTACT1667900.039.58091420-21
CGCAGAG1775900.038.82756816-17
TATCAAC1802400.038.46172710-11
AGTACTT1820400.038.37003720-21
GTACTTT1770150.037.23599222-23
Produced by FastQC (version 0.11.5)