##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660671_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48957781 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.416206649562 34.0 31.0 34.0 31.0 34.0 2 32.572456970629446 34.0 31.0 34.0 31.0 34.0 3 32.59577657328873 34.0 31.0 34.0 31.0 34.0 4 35.9533163277968 37.0 37.0 37.0 35.0 37.0 5 35.9513897903175 37.0 37.0 37.0 35.0 37.0 6 35.96582024418141 37.0 37.0 37.0 35.0 37.0 7 35.943350189829886 37.0 37.0 37.0 35.0 37.0 8 35.94763108238096 37.0 37.0 37.0 35.0 37.0 9 37.685283142224115 39.0 38.0 39.0 35.0 39.0 10-11 37.64042822937584 39.0 38.0 39.0 35.0 39.0 12-13 37.66858725684483 39.0 38.0 39.0 35.0 39.0 14-15 39.15461637242096 41.0 39.0 41.0 36.0 41.0 16-17 39.11280381559777 41.0 39.0 41.0 36.0 41.0 18-19 39.08171457975189 41.0 39.0 41.0 36.0 41.0 20-21 39.02955314702682 41.0 39.0 41.0 36.0 41.0 22-23 38.91990675598635 40.5 39.0 41.0 35.0 41.0 24-25 38.89429862027448 40.5 39.0 41.0 35.0 41.0 26-27 38.83687983121621 40.0 39.0 41.0 35.0 41.0 28-29 38.74855082790619 40.0 39.0 41.0 35.0 41.0 30-31 38.654463250693496 40.0 38.0 41.0 35.0 41.0 32-33 38.54809392811329 40.0 38.0 41.0 34.0 41.0 34-35 38.445019914199136 40.0 38.0 41.0 34.0 41.0 36-37 38.30883063307138 40.0 38.0 41.0 34.0 41.0 38-39 38.0421155117304 40.0 38.0 41.0 33.5 41.0 40-41 37.907138550662665 40.0 37.5 41.0 33.0 41.0 42-43 37.765246120938365 40.0 37.0 41.0 33.0 41.0 44-45 37.627368231824065 40.0 37.0 41.0 33.0 41.0 46-47 37.46355702886126 40.0 37.0 41.0 32.0 41.0 48-49 37.49071642973361 40.0 37.0 41.0 32.0 41.0 50-51 36.53741091737797 38.5 35.5 40.0 31.5 40.5 52-53 36.77273144793879 39.0 35.5 39.5 31.5 40.5 54-55 37.31819183144759 40.0 37.0 41.0 32.0 41.0 56-57 37.143820213175104 40.0 36.0 41.0 31.0 41.0 58-59 36.941865441164495 40.0 36.0 41.0 31.0 41.0 60-61 36.71846305697556 39.0 35.0 41.0 31.0 41.0 62-63 36.4500047704368 39.0 35.0 41.0 30.5 41.0 64-65 36.157072049486885 38.5 35.0 41.0 30.0 41.0 66-67 35.841780145223495 37.5 35.0 40.0 30.0 41.0 68-69 35.52173889376236 37.0 35.0 40.0 29.0 41.0 70-71 35.1703662386169 36.5 35.0 39.0 29.0 41.0 72-73 34.807141565505184 36.0 34.5 39.0 29.0 41.0 74-75 34.447075082916854 35.0 34.0 38.5 29.0 40.5 76-77 34.09043833093661 35.0 34.0 37.0 28.5 39.5 78-79 33.749103406055106 35.0 34.0 37.0 28.0 39.0 80-81 33.43231999219899 35.0 34.0 36.0 27.5 39.0 82-83 33.16041931107948 35.0 34.0 36.0 27.0 37.0 84-85 32.91818865319897 35.0 34.0 35.5 27.0 37.0 86-87 32.7105713287945 35.0 33.0 35.0 26.0 37.0 88-89 32.5206073882311 35.0 33.0 35.0 26.0 36.0 90-91 32.38717934336117 35.0 33.0 35.0 25.5 36.0 92-93 32.259870632208596 35.0 33.0 35.0 25.0 36.0 94-95 32.154763550251594 35.0 33.0 35.0 25.0 36.0 96-97 32.053850234756354 35.0 33.0 35.0 25.0 35.0 98-99 31.80945245455467 35.0 33.0 35.0 24.0 35.0 100 31.74288076904466 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.506564045547826 1101 2 0.49066100327750917 1101 3 0.48917663558627567 1101 4 0.42076670296316365 1101 5 0.421439975550733 1101 6 0.39202188348264144 1101 7 0.410779103788812 1101 8 0.41108516968354536 1101 9 0.5259108280647382 1101 10-11 0.48410488390543094 1101 12-13 0.48648193765737346 1101 14-15 0.6202772039070155 1101 16-17 0.643568216548779 1101 18-19 0.6180514198588156 1101 20-21 0.6318227469225235 1101 22-23 0.6411763788452163 1101 24-25 0.6694893545673821 1101 26-27 0.6443086903272786 1101 28-29 0.6460267268268041 1101 30-31 0.629229521821216 1101 32-33 0.6477970556441548 1101 34-35 0.6410907978320921 1101 36-37 0.6718727741139148 1101 38-39 0.6862058331765652 1101 40-41 0.7005227930547377 1101 42-43 0.7013091781314742 1101 44-45 0.6830050317033027 1101 46-47 0.6868740047005915 1101 48-49 0.69110445198951 1101 50-51 0.6408232158654457 1101 52-53 0.6843454776868185 1101 54-55 0.7294600706050716 1101 56-57 0.6974276920268423 1101 58-59 0.6619001020398159 1101 60-61 0.6637506236625086 1101 62-63 0.6535751984631304 1101 64-65 0.6482191043015533 1101 66-67 0.6519074542706846 1101 68-69 0.6142775587745248 1101 70-71 0.5987497029339863 1101 72-73 0.5493627847131108 1101 74-75 0.5587093444725681 1101 76-77 0.5055379152329706 1101 78-79 0.5358851561714673 1101 80-81 0.5390970447775061 1101 82-83 0.5198360554677137 1101 84-85 0.5317105867050671 1101 86-87 0.5352817265685488 1101 88-89 0.5182000887145435 1101 90-91 0.5149099973957547 1101 92-93 0.5174694689316368 1101 94-95 0.5028630212520113 1101 96-97 0.49624482268989567 1101 98-99 0.3116480953376346 1101 100 0.3996732527179532 1102 1 0.45997306619182865 1102 2 0.4359649785852753 1102 3 0.43981097694927485 1102 4 0.38613056986375227 1102 5 0.3784901406832546 1102 6 0.3403801009896057 1102 7 0.35459029091034466 1102 8 0.39045886877839564 1102 9 0.4580746082113052 1102 10-11 0.4817053848945463 1102 12-13 0.4619181180706704 1102 14-15 0.5371631493240443 1102 16-17 0.5905039695445993 1102 18-19 0.5880612448648463 1102 20-21 0.5474304040176463 1102 22-23 0.5624063498201437 1102 24-25 0.584854843248614 1102 26-27 0.5807720182055647 1102 28-29 0.5681613264914418 1102 30-31 0.5943244859215966 1102 32-33 0.6237473035876278 1102 34-35 0.5836442991772373 1102 36-37 0.6029599992383865 1102 38-39 0.6169074104071868 1102 40-41 0.5999155467882957 1102 42-43 0.621965012382482 1102 44-45 0.6035617547319987 1102 46-47 0.6102796198683436 1102 48-49 0.586567807767473 1102 50-51 0.6036870787812703 1102 52-53 0.6100966401920189 1102 54-55 0.6265098310467465 1102 56-57 0.6040314529205943 1102 58-59 0.5925263330942059 1102 60-61 0.5591155925693272 1102 62-63 0.5630115720780324 1102 64-65 0.5664379664410788 1102 66-67 0.5551008356376528 1102 68-69 0.5176333020410979 1102 70-71 0.4868932953021812 1102 72-73 0.4845617251512664 1102 74-75 0.4788950078734686 1102 76-77 0.4689615643936591 1102 78-79 0.4334520953564933 1102 80-81 0.4247517072414624 1102 82-83 0.4078019516717788 1102 84-85 0.4488267775491792 1102 86-87 0.44069285308357564 1102 88-89 0.42889407750241304 1102 90-91 0.40334609009362765 1102 92-93 0.43038588667211286 1102 94-95 0.43338000149982747 1102 96-97 0.40787679628913054 1102 98-99 0.2403250028085644 1102 100 0.26264380786548713 1103 1 0.39556184366844604 1103 2 0.40598649005981 1103 3 0.38999619309448263 1103 4 0.36213330293796275 1103 5 0.37886814243557154 1103 6 0.32796829899840674 1103 7 0.3512839957130609 1103 8 0.36516304525834187 1103 9 0.45532956984936135 1103 10-11 0.4404862843096211 1103 12-13 0.41700759376934116 1103 14-15 0.517435625259175 1103 16-17 0.5602696467716015 1103 18-19 0.5414788912559629 1103 20-21 0.5485138846757991 1103 22-23 0.5387000012798069 1103 24-25 0.5438540782118579 1103 26-27 0.5447689399369011 1103 28-29 0.5578911055283697 1103 30-31 0.5714726653380069 1103 32-33 0.5813125807329769 1103 34-35 0.5521768795532935 1103 36-37 0.5692415016373147 1103 38-39 0.5571762601968473 1103 40-41 0.5805641638953176 1103 42-43 0.6074622797554312 1103 44-45 0.6069793627729467 1103 46-47 0.5861093804817088 1103 48-49 0.5739943253460993 1103 50-51 0.49168907875434087 1103 52-53 0.5702089644152437 1103 54-55 0.5972312289734845 1103 56-57 0.5792386661980515 1103 58-59 0.5475350319336272 1103 60-61 0.5484436867064915 1103 62-63 0.49715040525260434 1103 64-65 0.5051548124043777 1103 66-67 0.5072578853766316 1103 68-69 0.4832606710043237 1103 70-71 0.4932741786996928 1103 72-73 0.4403099081487909 1103 74-75 0.4604128671014607 1103 76-77 0.45883686138815705 1103 78-79 0.43789884616979435 1103 80-81 0.4180466194633681 1103 82-83 0.42323259974264715 1103 84-85 0.4319715777071167 1103 86-87 0.4064256268818838 1103 88-89 0.413796100539642 1103 90-91 0.4292033663318193 1103 92-93 0.4389774527306116 1103 94-95 0.4264666998045854 1103 96-97 0.4225268009429115 1103 98-99 0.27835993560622185 1103 100 0.3290342221360021 1104 1 0.3625386815042262 1104 2 0.35200809653785115 1104 3 0.3328192282456257 1104 4 0.3011771537412713 1104 5 0.29440200072058076 1104 6 0.2701367377508461 1104 7 0.28292299202241367 1104 8 0.3050397494976025 1104 9 0.3582468865552002 1104 10-11 0.3793267844135144 1104 12-13 0.3604114828536211 1104 14-15 0.44536323812235423 1104 16-17 0.45367039463627634 1104 18-19 0.45293690079324733 1104 20-21 0.45429653912971446 1104 22-23 0.4647961661427189 1104 24-25 0.4778402688782748 1104 26-27 0.45655897033978476 1104 28-29 0.4696978075053053 1104 30-31 0.4756235843514105 1104 32-33 0.48354826574365717 1104 34-35 0.47504594706076375 1104 36-37 0.4778854986885577 1104 38-39 0.4874382493138043 1104 40-41 0.45743910814361044 1104 42-43 0.46367412665961183 1104 44-45 0.45946892727304345 1104 46-47 0.47183973435304694 1104 48-49 0.4667589719537375 1104 50-51 0.36447398657841745 1104 52-53 0.45367339707996734 1104 54-55 0.5097627730738878 1104 56-57 0.4967910299842302 1104 58-59 0.5119851095218948 1104 60-61 0.47803120037320923 1104 62-63 0.48424475047951887 1104 64-65 0.4774562596485268 1104 66-67 0.4556288909659756 1104 68-69 0.4694802530679567 1104 70-71 0.4604312329169886 1104 72-73 0.41248668459418525 1104 74-75 0.3859653889867616 1104 76-77 0.3791958958821553 1104 78-79 0.3735153681268528 1104 80-81 0.406417624220623 1104 82-83 0.3944175173264455 1104 84-85 0.4094550070280505 1104 86-87 0.40726366400625835 1104 88-89 0.4025467607170796 1104 90-91 0.3899877767264499 1104 92-93 0.4116375229446092 1104 94-95 0.3981458531248947 1104 96-97 0.38329400902576083 1104 98-99 0.2386337796780431 1104 100 0.31247226903955294 1105 1 0.3240366515787301 1105 2 0.3008494271763311 1105 3 0.3166451883179775 1105 4 0.2837223195078238 1105 5 0.2963559928017503 1105 6 0.2684844564182214 1105 7 0.27323807208548345 1105 8 0.28427468065074635 1105 9 0.34806492321412463 1105 10-11 0.33895283203982984 1105 12-13 0.36500790340469536 1105 14-15 0.4448078723958915 1105 16-17 0.4445520039896067 1105 18-19 0.43818915844227035 1105 20-21 0.4361175854069046 1105 22-23 0.4515877660802019 1105 24-25 0.45070013001576825 1105 26-27 0.41535901991231583 1105 28-29 0.41952084401468426 1105 30-31 0.4409800367989476 1105 32-33 0.4192496368972556 1105 34-35 0.43554754878491764 1105 36-37 0.4434562882024906 1105 38-39 0.4494091170967849 1105 40-41 0.4542144402703272 1105 42-43 0.46187207117267803 1105 44-45 0.47318694182712306 1105 46-47 0.4701097811236892 1105 48-49 0.4502462094518833 1105 50-51 0.3840706054280716 1105 52-53 0.42085621140755336 1105 54-55 0.48491383154182444 1105 56-57 0.46763299080265597 1105 58-59 0.4610560378003896 1105 60-61 0.4065985835321939 1105 62-63 0.4275399872260266 1105 64-65 0.44658088612087 1105 66-67 0.43870805254045564 1105 68-69 0.3935869691071119 1105 70-71 0.37308467271699897 1105 72-73 0.36520394154609903 1105 74-75 0.3951266938608242 1105 76-77 0.3962735202444847 1105 78-79 0.3975813692200987 1105 80-81 0.35688323556583157 1105 82-83 0.33888942082514717 1105 84-85 0.36043070668977606 1105 86-87 0.37087229067139305 1105 88-89 0.3624116127586845 1105 90-91 0.33896953342321723 1105 92-93 0.3558275460697615 1105 94-95 0.358958886243002 1105 96-97 0.3480089675990783 1105 98-99 0.27108949659518444 1105 100 0.2643077568811023 1106 1 0.31470542522833256 1106 2 0.31152969605636116 1106 3 0.3378378905455861 1106 4 0.2883355331195574 1106 5 0.2937612992172518 1106 6 0.274198862769623 1106 7 0.31124359401170665 1106 8 0.3007562029237576 1106 9 0.36408110111202063 1106 10-11 0.36195946720575023 1106 12-13 0.37690200048341893 1106 14-15 0.41862617041912387 1106 16-17 0.4711799086832329 1106 18-19 0.45152487213785264 1106 20-21 0.4709958902844136 1106 22-23 0.46938827046853504 1106 24-25 0.4534021626927611 1106 26-27 0.4503027576012215 1106 28-29 0.47103915038618993 1106 30-31 0.47497367476795205 1106 32-33 0.4729574286490319 1106 34-35 0.49973292148013826 1106 36-37 0.5063637787730286 1106 38-39 0.5007087587620305 1106 40-41 0.5214223971864342 1106 42-43 0.5512778725776286 1106 44-45 0.49391066246866444 1106 46-47 0.5604815631291018 1106 48-49 0.5537446057963749 1106 50-51 0.5063589497308811 1106 52-53 0.4954737108680263 1106 54-55 0.5162199558150604 1106 56-57 0.5175689394519836 1106 58-59 0.5097609645115639 1106 60-61 0.5193354631010578 1106 62-63 0.5097039155939314 1106 64-65 0.4745689844258152 1106 66-67 0.5108847256451341 1106 68-69 0.506892723702066 1106 70-71 0.49380826015486434 1106 72-73 0.4768318914939016 1106 74-75 0.47145586024901576 1106 76-77 0.4539881061594784 1106 78-79 0.4438837013858503 1106 80-81 0.47764221786622585 1106 82-83 0.4568933703452913 1106 84-85 0.47557320240692746 1106 86-87 0.4502326063698163 1106 88-89 0.4924154702515864 1106 90-91 0.46075663520753807 1106 92-93 0.46998665621696745 1106 94-95 0.49466448505354776 1106 96-97 0.4841236362053323 1106 98-99 0.3836825356486919 1106 100 0.4082321806872429 1107 1 0.3044062404922556 1107 2 0.2626951901989756 1107 3 0.25355977551799924 1107 4 0.27888122204556254 1107 5 0.28424559770395064 1107 6 0.24217406908904593 1107 7 0.22075994686888833 1107 8 0.25773349605928075 1107 9 0.3185025372925878 1107 10-11 0.3495825826292318 1107 12-13 0.3170179713827821 1107 14-15 0.3738748860266199 1107 16-17 0.37237019875517774 1107 18-19 0.3814867870327845 1107 20-21 0.373239000524066 1107 22-23 0.37670265309052553 1107 24-25 0.39476401702916775 1107 26-27 0.3787904595215963 1107 28-29 0.35981417968771723 1107 30-31 0.39042227554924835 1107 32-33 0.42196984792575876 1107 34-35 0.4018077822675892 1107 36-37 0.4216967626067003 1107 38-39 0.41745257447184514 1107 40-41 0.4464419359414933 1107 42-43 0.4238499880868787 1107 44-45 0.4416699937748376 1107 46-47 0.40957871758936193 1107 48-49 0.41852267246153474 1107 50-51 0.2992327305345128 1107 52-53 0.36636702015398726 1107 54-55 0.459562165597049 1107 56-57 0.4204679575314785 1107 58-59 0.4218929724628637 1107 60-61 0.4285361254184181 1107 62-63 0.4080848737217053 1107 64-65 0.3811886483115856 1107 66-67 0.38908566930103206 1107 68-69 0.4080758438804182 1107 70-71 0.3775402270220951 1107 72-73 0.3983657492266417 1107 74-75 0.37948064638830914 1107 76-77 0.3997606855618656 1107 78-79 0.38645211945377156 1107 80-81 0.3486341046710848 1107 82-83 0.36934110290847144 1107 84-85 0.4098636293672726 1107 86-87 0.4039908200107618 1107 88-89 0.39841258651879485 1107 90-91 0.37450015368988687 1107 92-93 0.40343451590861434 1107 94-95 0.38438112450999995 1107 96-97 0.39722364481249883 1107 98-99 0.281779755146804 1107 100 0.29969468185129955 1108 1 0.12521794468167258 1108 2 0.13980392655515317 1108 3 0.13371116394510096 1108 4 0.14791583013344933 1108 5 0.15401188956661827 1108 6 0.1425883488956785 1108 7 0.11465228065473099 1108 8 0.13352403759991915 1108 9 0.16324275003656652 1108 10-11 0.17352301005596615 1108 12-13 0.1526878802092213 1108 14-15 0.14998490108646223 1108 16-17 0.18640271772919448 1108 18-19 0.17095817842945848 1108 20-21 0.18100519349413702 1108 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#Quality Count 2 1364.0 3 461.0 4 496.0 5 396.0 6 1191.0 7 9220.0 8 19503.0 9 24930.0 10 28675.0 11 29811.0 12 31712.0 13 36640.0 14 45470.0 15 60667.0 16 82089.0 17 102842.0 18 112408.0 19 123596.0 20 140419.0 21 168999.0 22 216059.0 23 305784.0 24 422894.0 25 518766.0 26 540081.0 27 496189.0 28 478912.0 29 517758.0 30 603729.0 31 740997.0 32 930186.0 33 1222137.0 34 1736304.0 35 2817886.0 36 5324039.0 37 1.1538385E7 38 1.5017949E7 39 4499027.0 40 9810.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.649258423451037 30.970077177827587 27.965510552359845 23.415153846361527 2 22.328120807385314 30.356505466582007 27.11294496510212 20.20242876093056 3 23.58566708554758 25.974389461026696 27.066132962081895 23.37381049134382 4 20.54698419092927 27.666435913221715 25.78443463695743 26.00214525889159 5 22.54808298783619 29.373951751913125 26.755133233076695 21.322832027173995 6 25.712198199871157 27.066430320388463 26.304456019448363 20.91691546029202 7 22.168549509884073 26.407246263705055 29.454286598796397 21.96991762761448 8 24.861056759465786 26.83784659178571 26.372261826549053 21.92883482219945 9 24.330651189560225 25.901881775136694 27.572710966786275 22.194756068516813 10-11 21.798808134273457 28.359737028629617 28.02783362716703 21.813621209929895 12-13 21.750107228212677 26.92752690480497 27.950065927309613 23.372299939672736 14-15 21.74769225112189 29.8553754525486 26.52770856304425 21.86922373328526 16-17 23.343963850894646 26.72373400474663 26.56898466699827 23.36331747736046 18-19 21.822778284719483 28.231005839476108 26.569475841230044 23.376740034574365 20-21 23.341380719870198 28.295433389484742 26.522784836699103 21.840401053945957 22-23 23.313732867936984 26.84234781259 27.97902192300367 21.864897396469342 24-25 21.92069915858218 28.131594955515602 26.417353537537398 23.530352348364815 26-27 21.972481556228825 26.696696560810967 29.429789278146597 21.90103260481361 28-29 22.004009509128956 26.775600759098428 29.36374367207301 21.856646059699607 30-31 21.96903857276567 26.7119209178141 29.372144762395365 21.94689574702487 32-33 22.052358416932716 26.81643674697623 29.22102004224914 21.910184793841914 34-35 22.0706942419641 26.926978902208965 29.140442218534712 21.86188463729222 36-37 22.041606247299185 26.903078446971623 29.162789771793708 21.892525533935483 38-39 22.103295115012624 26.90117688683719 29.166517066057818 21.82901093209237 40-41 22.147272912079295 26.97973818852694 29.116359462892678 21.75662943650109 42-43 22.184522034403674 26.994987340939915 29.03817815939092 21.78231246526549 44-45 22.262754831672616 27.062828790861747 28.977846720091282 21.69656965737435 46-47 22.327071138163376 27.16296791062542 28.859851738660375 21.650109212550824 48-49 22.430832116888535 27.144071936196095 28.76840549303152 21.656690453883854 50-51 22.518010898971855 27.1845007809387 28.700868325870648 21.596619994218795 52-53 22.668215010164158 27.281044131007366 28.51565477077407 21.53508608805441 54-55 22.938110279544897 27.40304881974846 28.06490869906797 21.593932201638673 56-57 23.26879766024915 27.67730972948807 27.509140443507274 21.544752166755508 58-59 23.4819604420131 27.90140292106252 27.184177630635713 21.43245900628867 60-61 23.652339911895638 27.991128292450863 27.008256902167243 21.34827489348626 62-63 23.826169454002496 28.0577833689835 26.88474916184186 21.23129801517214 64-65 24.021883733707718 28.116860358209593 26.801054901713556 21.060201006369134 66-67 24.192848883389175 28.02237997319188 26.8292475630155 20.955523580403444 68-69 24.37170132238243 27.979521204598967 26.858759953622126 20.790017519396475 70-71 24.56304123387045 27.939237406302563 26.86484939540612 20.63287196442087 72-73 24.759865880014743 27.88177572123964 26.797993805258624 20.560364593486995 74-75 24.977232732447483 27.900044752927204 26.71330035704053 20.409422157584782 76-77 25.24936819184445 27.932329208833483 26.528460483442167 20.289842115879896 78-79 25.487399249734626 27.944936771253246 26.33596992522469 20.23169405378744 80-81 25.701629985315687 27.966532695942604 26.182767508895626 20.14906980984608 82-83 25.882327952968254 27.999136343100396 26.026649458913887 20.091886245017466 84-85 26.040920873048673 27.920820135924764 25.95397498298828 20.084284008038285 86-87 26.220861257472034 27.859120532154837 25.893180795575894 20.026837414797235 88-89 26.360466947801005 27.82024048909638 25.844402967588053 19.974889595514558 90-91 26.46421393433315 27.747931911612874 25.816588466541063 19.971265687512922 92-93 26.553497369243278 27.76062827789756 25.795770820439284 19.890103532419882 94-95 26.609330847038475 27.798720411686094 25.772543823153608 19.81940491812182 96-97 26.641532526654327 27.843363696175427 25.715210435336957 19.799893341833293 98-99 26.69488310326093 27.954645927826533 25.631700968268113 19.718770000644426 100 26.689090914595592 28.082608562951123 25.535220244305762 19.69308027814752 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 100195.0 1 62141.0 2 18769.5 3 12362.0 4 11075.5 5 10812.5 6 10898.0 7 11098.0 8 10922.0 9 10927.0 10 11718.0 11 16515.0 12 122181.5 13 142541.0 14 59792.0 15 61463.0 16 68991.0 17 76945.0 18 84929.5 19 93533.0 20 106174.5 21 126526.5 22 154588.5 23 188523.5 24 224328.5 25 265858.5 26 315475.0 27 372336.5 28 444993.5 29 521568.0 30 588864.0 31 662651.5 32 744354.0 33 820851.0 34 896197.5 35 991574.5 36 1101262.0 37 1182979.5 38 1254004.5 39 1328249.5 40 1386427.0 41 1449764.5 42 1523238.0 43 1574110.5 44 1615170.0 45 1678042.0 46 1744413.5 47 1804124.5 48 1847586.5 49 1870145.5 50 1878492.0 51 1870253.0 52 1840925.0 53 1786706.5 54 1707091.0 55 1603425.5 56 1478258.0 57 1337559.0 58 1181192.0 59 1008243.5 60 829699.0 61 664576.5 62 519423.5 63 391031.0 64 287833.0 65 210264.5 66 152385.5 67 111749.0 68 82904.5 69 62917.0 70 49322.0 71 39599.5 72 32161.5 73 24926.0 74 19464.5 75 16101.5 76 12261.0 77 7647.0 78 4870.0 79 3568.0 80 2481.5 81 1772.5 82 1303.0 83 993.0 84 796.5 85 618.5 86 507.0 87 410.0 88 345.5 89 312.0 90 272.5 91 216.0 92 169.5 93 148.5 94 131.0 95 104.0 96 85.0 97 65.0 98 47.5 99 35.0 100 17.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13590689496323374 2 0.051086874219238 3 0.018595614045497692 4 0.0753281689789004 5 0.07896599725383796 6 0.08114542609682412 7 0.04808632972969097 8 0.04561276990883227 9 0.0333613976499466 10-11 0.022795150785122387 12-13 0.014864848551857365 14-15 0.011961326433483577 16-17 0.016950318888023133 18-19 0.01619252310475428 20-21 0.022975918781939893 22-23 0.019483113419703397 24-25 0.016748103840735756 26-27 0.015212086511845788 28-29 0.017152533935310507 30-31 0.015092595802085065 32-33 0.012285074766766902 34-35 0.009829898132025224 36-37 0.007685193085037902 38-39 0.01634571632239623 40-41 0.02503381433893011 42-43 0.02533100918115549 44-45 0.024242316047780026 46-47 0.026261402656300947 48-49 0.022844172614767812 50-51 0.022953450443352407 52-53 0.019517837215702237 54-55 0.02435261516448223 56-57 0.030912348743910596 58-59 0.023662224396975833 60-61 0.023280262640988567 62-63 0.030736687187681157 64-65 0.03145873788683355 66-67 0.024078909948961943 68-69 0.024574234686004254 70-71 0.03555716710281456 72-73 0.04015807007266118 74-75 0.04126003995156561 76-77 0.04219043342671107 78-79 0.0365570081699577 80-81 0.032160362823633694 82-83 0.03482592481060365 84-85 0.03824213356401917 86-87 0.03807566360084825 88-89 0.03739242185016514 90-91 0.040388881187241724 92-93 0.0335891449001743 94-95 0.03352888890123513 96-97 0.03345944130923745 98-99 0.3159170959974677 100 0.15072782812603375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.8957781E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.01134689856928 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95318626723213 34.63766562024603 2 10.622113929754978 9.562313097766523 3 4.106192977752694 5.544758296622871 4 2.1631674352626913 3.894683193131896 5 1.3399764604469975 3.0157072648548398 6 0.9027501607447006 2.438040038881134 7 0.6650783080085189 2.095524930553947 8 0.4758386684011528 1.7134511496926055 9 0.3675147086960505 1.4888098839100112 >10 2.1247071670454303 17.404109766737154 >50 0.1635451525477486 5.122629972173496 >100 0.11452904501904444 9.599788251588906 >500 0.0010653548616944288 0.2967446029481279 >1k 2.8910521079124584E-4 0.25774792871138386 >5k 2.7155457539243814E-5 0.07142530055128223 >10k+ 1.810355770647006E-5 2.8566007016295645 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1244644 2.542280255716655 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 71013 0.1450494661921054 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 52141 0.10650196748091993 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.007379827937871612 1.2255457411356124E-5 0.0 2.0425762352260206E-6 6.127728705678062E-6 2 0.007387998242812516 1.8383186117034185E-5 0.0 2.0425762352260206E-6 6.127728705678062E-6 3 0.0073941259715181945 1.8383186117034185E-5 0.0 4.085152470452041E-6 1.0212881176130103E-5 4 0.0073941259715181945 2.859606729316429E-5 0.0 4.085152470452041E-6 2.0425762352260206E-5 5 0.007402296276459099 3.880894846929439E-5 0.0 1.0212881176130103E-5 2.6553491057938268E-5 6 0.007406381428929551 4.289410093974643E-5 0.0 1.4298033646582144E-5 2.859606729316429E-5 7 0.007408424005164777 4.493667717497245E-5 0.0 1.4298033646582144E-5 3.268121976361633E-5 8 0.007410466581400003 4.9021829645424494E-5 0.0 1.4298033646582144E-5 3.472379599884235E-5 9 0.007412509157635229 5.9234710821554594E-5 2.0425762352260206E-6 1.6340609881808165E-5 3.676637223406837E-5 10-11 0.0074176155982232945 7.455403258574975E-5 2.0425762352260206E-6 1.8383186117034185E-5 3.8808948469294395E-5 12-13 0.007427828479399424 9.702237117323597E-5 2.0425762352260206E-6 2.0425762352260206E-5 4.4936677174972446E-5 14-15 0.007443147801163619 1.1438426917265715E-4 2.0425762352260206E-6 2.2468338587486227E-5 5.9234710821554594E-5 16-17 0.0074564245466925885 1.2357586223117424E-4 2.0425762352260206E-6 3.0638643528390306E-5 6.842630388007168E-5 18-19 0.007493190918926656 1.378738958777564E-4 2.0425762352260206E-6 3.778766035168138E-5 7.353274446813674E-5 20-21 0.0075381275961016285 1.491080651714995E-4 2.0425762352260206E-6 3.880894846929439E-5 7.863918505620178E-5 22-23 0.007770981286917395 1.6544867505330766E-4 2.0425762352260206E-6 4.187281282213342E-5 8.680948999710588E-5 24-25 0.009047591433933657 1.7566155622943778E-4 2.0425762352260206E-6 5.106440588065051E-5 9.702237117323598E-5 26-27 0.01425616083376001 1.9200216611124594E-4 2.0425762352260206E-6 5.514955835110255E-5 1.0621396423175307E-4 28-29 0.029787910526418673 2.0527891164021507E-4 2.0425762352260206E-6 5.719213458632858E-5 1.1029911670220512E-4 30-31 0.0663071310360247 2.185556571691842E-4 2.0425762352260206E-6 5.9234710821554594E-5 1.1744813352549619E-4 32-33 0.1363297082439255 2.3183240269815332E-4 2.0425762352260206E-6 5.9234710821554594E-5 1.2561843846640026E-4 34-35 0.2518087982786638 2.461304363447355E-4 2.0425762352260206E-6 6.229857517439362E-5 1.3685260776014337E-4 36-37 0.4217174385415875 2.604284699913176E-4 2.0425762352260206E-6 6.638372764484567E-5 1.491080651714995E-4 38-39 0.6503930396681991 2.7268392740267377E-4 2.0425762352260206E-6 7.046888011529772E-5 1.6749125128853368E-4 40-41 0.9411312984140355 2.859606729316429E-4 2.0425762352260206E-6 7.455403258574975E-5 1.7464026811182478E-4 42-43 1.291863085052813 2.98216130342999E-4 2.0425762352260206E-6 8.170304940904082E-5 1.8485314928795484E-4 44-45 1.7030704475760452 3.1455674022480714E-4 2.0425762352260206E-6 8.783077811471889E-5 1.9200216611124594E-4 46-47 2.1769675386227165 3.390676550475194E-4 2.0425762352260206E-6 8.98733543499449E-5 2.063001997578281E-4 48-49 2.71201731957582 3.5438697681171457E-4 2.0425762352260206E-6 9.089464246755791E-5 2.1753436905157117E-4 50-51 3.304303150504309 3.6357856987023166E-4 2.0425762352260206E-6 9.191593058517092E-5 2.2774725022770128E-4 52-53 3.94651056590984 3.697062985759097E-4 2.0425762352260206E-6 9.191593058517092E-5 2.4408786010950948E-4 54-55 4.6325669866450845 3.788978916344268E-4 2.0425762352260206E-6 9.395850682039694E-5 2.5736460563847855E-4 56-57 5.36161044553878 3.9115334904578293E-4 2.0425762352260206E-6 9.702237117323597E-5 2.634923343441567E-4 58-59 6.135386323983925 4.085152470452041E-4 2.0425762352260206E-6 1.00086235526075E-4 2.7064135116744773E-4 60-61 6.945976575204664 4.248558569270123E-4 2.0425762352260206E-6 1.00086235526075E-4 2.798329442259648E-4 62-63 7.789147959953496 4.3915389057359443E-4 2.0425762352260206E-6 1.0315009987891405E-4 2.9310968975493394E-4 64-65 8.656917069015035 4.463029073968855E-4 2.0425762352260206E-6 1.1029911670220512E-4 2.97194842225386E-4 66-67 9.548919302531298 4.5447321233778955E-4 2.0425762352260206E-6 1.1540555729027016E-4 3.0332257093106406E-4 68-69 10.46745664391938 4.575370766906286E-4 2.0425762352260206E-6 1.2561843846640026E-4 3.1557802834242016E-4 70-71 11.404726451960721 4.6877124598437175E-4 2.0425762352260206E-6 1.3072487905446532E-4 3.186418926952592E-4 72-73 12.359280540104544 4.8409056774856686E-4 2.0425762352260206E-6 1.3276745528969134E-4 3.278334857537763E-4 74-75 13.327600570785673 4.973673132775359E-4 2.0425762352260206E-6 1.3787389587775636E-4 3.3396121445945434E-4 76-77 14.309357689230236 5.045163301008271E-4 2.0425762352260206E-6 1.4093776023059543E-4 3.4825924810603654E-4 78-79 15.305656929181492 5.157504993945701E-4 2.0425762352260206E-6 1.491080651714995E-4 3.584721292821666E-4 80-81 16.312616374504394 5.280059568059264E-4 2.0425762352260206E-6 1.6238481070046863E-4 3.758340272815878E-4 82-83 17.329603847854134 5.412827023348955E-4 2.0425762352260206E-6 1.6953382752375973E-4 3.8808948469294387E-4 84-85 18.350737750961386 5.586446003343166E-4 2.0425762352260206E-6 1.7464026811182478E-4 3.99323653986687E-4 86-87 19.37587510348968 5.790703626865768E-4 2.0425762352260206E-6 1.777041324646638E-4 4.085152470452041E-4 88 20.149211828044248 5.923471082155459E-4 2.0425762352260206E-6 1.8178928493511583E-4 4.1464297575088217E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 173195 0.0 80.21115 8 GTGGTAT 177065 0.0 78.631714 6 GTATCAA 176845 0.0 78.389046 9 TGGTATC 177975 0.0 77.987305 7 AGTGGTA 179065 0.0 77.84965 5 AAGCAGT 190915 0.0 73.810326 1 GCAGTGG 194815 0.0 72.268524 3 CAGTGGT 196320 0.0 71.31695 4 AGCAGTG 206485 0.0 68.45404 2 CAACGCA 167765 0.0 43.568424 12-13 ATCAACG 170480 0.0 42.887848 10-11 ACGCAGA 174720 0.0 41.863037 14-15 TCAACGC 167055 0.0 41.500805 12-13 AGAGTAC 175570 0.0 40.999344 18-19 AACGCAG 170925 0.0 40.482037 14-15 GAGTACT 166790 0.0 39.580914 20-21 CGCAGAG 177590 0.0 38.827568 16-17 TATCAAC 180240 0.0 38.461727 10-11 AGTACTT 182040 0.0 38.370037 20-21 GTACTTT 177015 0.0 37.235992 22-23 >>END_MODULE