FastQCFastQC Report
Thu 26 May 2016
SRR1660671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48957781
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11822382.4148112431811404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA668660.1365789025446231No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT558200.11401660545031647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1642850.079.020118
GTGGTAT1683250.077.240756
GTATCAA1684950.076.861719
AGTGGTA1704300.076.385985
TGGTATC1701050.076.3165057
AAGCAGT1811250.072.452131
GCAGTGG1845150.070.934393
CAGTGGT1879350.069.476064
AGCAGTG1968550.066.779792
CAACGCA1586150.043.05653412-13
ATCAACG1617950.042.3189910-11
ACGCAGA1653350.041.28796814-15
TCAACGC1574900.040.93749612-13
AGAGTAC1679600.040.33004418-19
AACGCAG1614750.039.79177514-15
GAGTACT1589300.038.90963720-21
CGCAGAG1679900.038.18947616-17
TATCAAC1715550.037.73989510-11
AGTACTT1752550.037.53585420-21
GTACTTT1694200.036.46816322-23
Produced by FastQC (version 0.11.5)