##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660671_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	48957781
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.799065790992444	34.0	31.0	34.0	31.0	34.0
2	32.97912139849639	34.0	31.0	34.0	31.0	34.0
3	33.03185444618089	34.0	33.0	34.0	31.0	34.0
4	36.40538150615936	37.0	37.0	37.0	35.0	37.0
5	36.33447006105117	37.0	37.0	37.0	35.0	37.0
6	36.32728517658919	37.0	37.0	37.0	35.0	37.0
7	36.29569753171615	37.0	37.0	37.0	35.0	37.0
8	36.301928635205094	37.0	37.0	37.0	35.0	37.0
9	38.08652367230451	39.0	39.0	39.0	37.0	39.0
10-11	38.04744301013152	39.0	38.0	39.0	36.0	39.0
12-13	38.067012330481234	39.0	38.0	39.0	36.0	39.0
14-15	39.58265670782751	41.0	40.0	41.0	37.0	41.0
16-17	39.55627966471765	41.0	40.0	41.0	37.0	41.0
18-19	39.525049603453226	41.0	39.5	41.0	37.0	41.0
20-21	39.49680202009156	41.0	39.5	41.0	37.0	41.0
22-23	39.39422879480587	41.0	39.0	41.0	36.5	41.0
24-25	39.375950740087674	41.0	39.0	41.0	36.0	41.0
26-27	39.30724530795217	41.0	39.0	41.0	36.0	41.0
28-29	39.22691682043351	41.0	39.0	41.0	36.0	41.0
30-31	39.14027929492964	40.0	39.0	41.0	36.0	41.0
32-33	39.04766014619821	40.0	39.0	41.0	35.0	41.0
34-35	38.95038455684909	40.0	38.5	41.0	35.0	41.0
36-37	38.822864204159906	40.0	38.0	41.0	35.0	41.0
38-39	38.59463536552035	40.0	38.0	41.0	35.0	41.0
40-41	38.479061663354386	40.0	38.0	41.0	35.0	41.0
42-43	38.35509621034499	40.0	38.0	41.0	34.0	41.0
44-45	38.23111093413323	40.0	38.0	41.0	34.0	41.0
46-47	38.097776296274546	40.0	37.5	41.0	34.0	41.0
48-49	37.946124539427146	40.0	37.0	41.0	33.0	41.0
50-51	38.004905737047196	40.0	37.0	41.0	33.5	41.0
52-53	37.95547067380362	40.0	37.0	41.0	33.5	41.0
54-55	37.60682093618581	40.0	37.0	41.0	33.0	41.0
56-57	37.40113448973514	40.0	36.5	41.0	33.0	41.0
58-59	37.19697007917904	39.5	36.0	41.0	32.5	41.0
60-61	36.973088516818194	39.0	35.5	41.0	32.0	41.0
62-63	36.71719101198643	39.0	35.0	41.0	32.0	41.0
64-65	36.44455685195373	38.5	35.0	40.5	31.0	41.0
66-67	36.15815809748403	37.5	35.0	40.0	31.0	41.0
68-69	35.84767856410812	37.0	35.0	40.0	31.0	41.0
70-71	35.51322473132514	36.5	35.0	39.0	31.0	41.0
72-73	35.15579599083545	36.0	35.0	39.0	30.5	41.0
74-75	34.807193283535454	35.5	34.5	38.5	30.0	40.0
76-77	32.94472697812836	34.5	32.0	36.0	27.5	39.0
78-79	34.08989858016645	35.0	34.0	37.0	30.0	39.0
80-81	34.05811316284944	35.0	34.0	36.5	30.0	39.0
82-83	33.83222700595846	35.0	34.0	36.0	30.0	37.5
84-85	33.60493953964131	35.0	34.0	36.0	30.0	37.0
86-87	33.41205239673751	35.0	34.0	35.0	30.0	37.0
88-89	33.246432472092636	35.0	34.0	35.0	30.0	36.0
90-91	33.13357455886328	35.0	34.0	35.0	29.0	36.0
92-93	33.03819457217638	35.0	34.0	35.0	29.0	36.0
94-95	32.942098989331235	35.0	34.0	35.0	29.0	35.5
96-97	32.870218964785195	35.0	34.0	35.0	29.0	35.0
98-99	32.779968704055435	35.0	34.0	35.0	29.0	35.0
100	32.73202723383235	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.37575582984705136
1101	2	0.3234983506958926
1101	3	0.3157136707435413
1101	4	0.22638869719602184
1101	5	0.23132299905896048
1101	6	0.24877547811814793
1101	7	0.24691950790893458
1101	8	0.25673367793401525
1101	9	0.3087660158853325
1101	10-11	0.3134524605007911
1101	12-13	0.3219505572024204
1101	14-15	0.40057602476473164
1101	16-17	0.4272089713826972
1101	18-19	0.4175595673474888
1101	20-21	0.42091089332955534
1101	22-23	0.42775697619811126
1101	24-25	0.42629704647546873
1101	26-27	0.4523107460743958
1101	28-29	0.45532040407167784
1101	30-31	0.46140193922494177
1101	32-33	0.46729886779854723
1101	34-35	0.4687350103725052
1101	36-37	0.49297802898560406
1101	38-39	0.47647681596775726
1101	40-41	0.47196680800963264
1101	42-43	0.5090484455790474
1101	44-45	0.48873546454645833
1101	46-47	0.4816565332247862
1101	48-49	0.5026546505878642
1101	50-51	0.43453921125001216
1101	52-53	0.46589162620764313
1101	54-55	0.3542645484908036
1101	56-57	0.36241718739429984
1101	58-59	0.38029497308471605
1101	60-61	0.3581154132054323
1101	62-63	0.3072971302912393
1101	64-65	0.3458818114341611
1101	66-67	0.3313115805936917
1101	68-69	0.3362189755799321
1101	70-71	0.32902851800460553
1101	72-73	0.355810740643939
1101	74-75	0.34456472928739146
1101	76-77	0.36175971168592724
1101	78-79	0.4066725561828761
1101	80-81	0.38840447606560957
1101	82-83	0.3891720859177923
1101	84-85	0.3613938479543961
1101	86-87	0.39355549194915795
1101	88-89	0.42151397142765745
1101	90-91	0.3791701211398504
1101	92-93	0.3465691001595772
1101	94-95	0.336320616572074
1101	96-97	0.3411666059492191
1101	98-99	0.3367165663218259
1101	100	0.35831784385158016
1102	1	0.3204395873741177
1102	2	0.2740071498451542
1102	3	0.2792782886331793
1102	4	0.2067050392630776
1102	5	0.2218228740152952
1102	6	0.22664671968796313
1102	7	0.24017925540923102
1102	8	0.22450388205358252
1102	9	0.2866567306789136
1102	10-11	0.30569202714917054
1102	12-13	0.2930466911602494
1102	14-15	0.36768423852089427
1102	16-17	0.38331939394501546
1102	18-19	0.3893931054510702
1102	20-21	0.4030373833116627
1102	22-23	0.41095362792901824
1102	24-25	0.3928030779625047
1102	26-27	0.4175076871634431
1102	28-29	0.43499837095564686
1102	30-31	0.4183797235250566
1102	32-33	0.4330106510572378
1102	34-35	0.43314495273954634
1102	36-37	0.4425463801964966
1102	38-39	0.4370036349293258
1102	40-41	0.4342009120244441
1102	42-43	0.41594670068315764
1102	44-45	0.45273543347561684
1102	46-47	0.4583323392583125
1102	48-49	0.4645099922078728
1102	50-51	0.45221591355604573
1102	52-53	0.46192686426224583
1102	54-55	0.3527786661187733
1102	56-57	0.35697458220925427
1102	58-59	0.3560894042658518
1102	60-61	0.27716693683176175
1102	62-63	0.3096540690916001
1102	64-65	0.30557758879128016
1102	66-67	0.2782641408679325
1102	68-69	0.28272076529314916
1102	70-71	0.3117696217871142
1102	72-73	0.3063409043228589
1102	74-75	0.33747770507095254
1102	76-77	0.3306023381960088
1102	78-79	0.3614195027291416
1102	80-81	0.3500751391092436
1102	82-83	0.35260837442360327
1102	84-85	0.38915489832665884
1102	86-87	0.37849704737018897
1102	88-89	0.3905321130555066
1102	90-91	0.3533671431810532
1102	92-93	0.30991953057174015
1102	94-95	0.34540071684788387
1102	96-97	0.3573890582156096
1102	98-99	0.3734576306210471
1102	100	0.3807962777308376
1103	1	0.276770880074622
1103	2	0.2502211400592813
1103	3	0.24180921836494917
1103	4	0.16563671578255423
1103	5	0.17065218643108437
1103	6	0.17518697705332897
1103	7	0.19577776467337316
1103	8	0.15927660938070431
1103	9	0.2360856017488615
1103	10-11	0.25027246434245143
1103	12-13	0.25036768196063974
1103	14-15	0.3244570461317977
1103	16-17	0.31491089478738843
1103	18-19	0.31207208138732767
1103	20-21	0.3308020778840941
1103	22-23	0.33049944701707545
1103	24-25	0.34278609593467735
1103	26-27	0.3171964032728667
1103	28-29	0.33785790911300495
1103	30-31	0.3545677352249612
1103	32-33	0.3238723242855883
1103	34-35	0.3563153655393805
1103	36-37	0.3496537633553132
1103	38-39	0.36696246190343373
1103	40-41	0.3170251327352318
1103	42-43	0.3514345391462186
1103	44-45	0.3574342972531568
1103	46-47	0.34372160530859475
1103	48-49	0.3196924873177096
1103	50-51	0.35115146370665684
1103	52-53	0.34317744681077045
1103	54-55	0.2311706132847462
1103	56-57	0.24111533298450638
1103	58-59	0.2535398595599858
1103	60-61	0.20555258627423
1103	62-63	0.18860401684761285
1103	64-65	0.17308601699874515
1103	66-67	0.17269980577432875
1103	68-69	0.17952693271027442
1103	70-71	0.17626259758423402
1103	72-73	0.21579548912246338
1103	74-75	0.23224499739746562
1103	76-77	0.15140314269005017
1103	78-79	0.3058440527257531
1103	80-81	0.2954968448308293
1103	82-83	0.2934521267334276
1103	84-85	0.29049791192790764
1103	86-87	0.30124359644141663
1103	88-89	0.2988532762676499
1103	90-91	0.25252059334668076
1103	92-93	0.22956875251156106
1103	94-95	0.23675155837225503
1103	96-97	0.2667768867726963
1103	98-99	0.2638650773702196
1103	100	0.28764316861061445
1104	1	0.22923946008194918
1104	2	0.1946979759033951
1104	3	0.18557900979065067
1104	4	0.14580718870766418
1104	5	0.15133024477704993
1104	6	0.1541212769099971
1104	7	0.1600004076510544
1104	8	0.15938834878840424
1104	9	0.20270215356161003
1104	10-11	0.19829011695146193
1104	12-13	0.20323708477179991
1104	14-15	0.2322818733914147
1104	16-17	0.26716469861632675
1104	18-19	0.2558503438144797
1104	20-21	0.2619831841525482
1104	22-23	0.2715380769836955
1104	24-25	0.29035874154273955
1104	26-27	0.2582500884247949
1104	28-29	0.2801447659103147
1104	30-31	0.26058352522228034
1104	32-33	0.2621296347043298
1104	34-35	0.2984541040622801
1104	36-37	0.30847779611797677
1104	38-39	0.26520631945880524
1104	40-41	0.2894462214196949
1104	42-43	0.27363992558803574
1104	44-45	0.2796790018592006
1104	46-47	0.26535727543849674
1104	48-49	0.2873089169668859
1104	50-51	0.25111088374721646
1104	52-53	0.2479249889647619
1104	54-55	0.1595312195855172
1104	56-57	0.1850105977811083
1104	58-59	0.19256870830798078
1104	60-61	0.14822588142110504
1104	62-63	0.12970284056879677
1104	64-65	0.16192159043406917
1104	66-67	0.14766845748888358
1104	68-69	0.10031174705029855
1104	70-71	0.12672562906256246
1104	72-73	0.16324424485836886
1104	74-75	0.18394016265904156
1104	76-77	0.1567050197693547
1104	78-79	0.23147311441304197
1104	80-81	0.23326363102271586
1104	82-83	0.2160728213522063
1104	84-85	0.2163030433270805
1104	86-87	0.23004092652388408
1104	88-89	0.2784250787201117
1104	90-91	0.24629348270088514
1104	92-93	0.2316104879084122
1104	94-95	0.2481759069934597
1104	96-97	0.2612301149921663
1104	98-99	0.24836637213891777
1104	100	0.2732999263628244
1105	1	0.18134744325612218
1105	2	0.1525413723886757
1105	3	0.13592961705199258
1105	4	0.11882315501384255
1105	5	0.10169619528594609
1105	6	0.11878304230314995
1105	7	0.12084063170727433
1105	8	0.13758235525293117
1105	9	0.16726309138265094
1105	10-11	0.16628433505938034
1105	12-13	0.14935568110396247
1105	14-15	0.19701431569195904
1105	16-17	0.1880225305553438
1105	18-19	0.22910664736225073
1105	20-21	0.21749641133457231
1105	22-23	0.21243751992997773
1105	24-25	0.22138595772636904
1105	26-27	0.22973430320086408
1105	28-29	0.21952665605684984
1105	30-31	0.21856559016982402
1105	32-33	0.21755379933086516
1105	34-35	0.2345636981441359
1105	36-37	0.2144385158788893
1105	38-39	0.20965131956965166
1105	40-41	0.2268955462419271
1105	42-43	0.2295875040181059
1105	44-45	0.2601801209019712
1105	46-47	0.22087019776041217
1105	48-49	0.20283740977598796
1105	50-51	0.2057116434393791
1105	52-53	0.21331851446834804
1105	54-55	0.11300290118842327
1105	56-57	0.10003699374222919
1105	58-59	0.09380672516007138
1105	60-61	0.07620383797048191
1105	62-63	0.012071720047536871
1105	64-65	0.06941103947632854
1105	66-67	0.06963469283468271
1105	68-69	0.03216937504203088
1105	70-71	0.06053431576116708
1105	72-73	0.10605264817245086
1105	74-75	0.10735638088673483
1105	76-77	0.06135781680850272
1105	78-79	0.19185265350839842
1105	80-81	0.2068237547959697
1105	82-83	0.21178528351290993
1105	84-85	0.2159473175678599
1105	86-87	0.21928420292797313
1105	88-89	0.21342341352769978
1105	90-91	0.21300414648559496
1105	92-93	0.1565085177051131
1105	94-95	0.19182087073067322
1105	96-97	0.20560108189251025
1105	98-99	0.23329042307899783
1105	100	0.22619632893200503
1106	1	0.21607177076943174
1106	2	0.18175399874722586
1106	3	0.1794376194360865
1106	4	0.13410986444677064
1106	5	0.1319163855167318
1106	6	0.1386822223441868
1106	7	0.14275679261833574
1106	8	0.1563397480362525
1106	9	0.20141595924737743
1106	10-11	0.19122267252563319
1106	12-13	0.1896542379652928
1106	14-15	0.23491874912586752
1106	16-17	0.24031171661112438
1106	18-19	0.24224375799735043
1106	20-21	0.23406989126480227
1106	22-23	0.24769083944853065
1106	24-25	0.2700742882242153
1106	26-27	0.2629100560955564
1106	28-29	0.24916936617274388
1106	30-31	0.23480818034015982
1106	32-33	0.27388023228763103
1106	34-35	0.27493538168513254
1106	36-37	0.24593079543617336
1106	38-39	0.2707057319792412
1106	40-41	0.2654419106976391
1106	42-43	0.2757373741415634
1106	44-45	0.30468352458348846
1106	46-47	0.3039825951854027
1106	48-49	0.2683034538731235
1106	50-51	0.2672867537201
1106	52-53	0.28266978150042377
1106	54-55	0.22025563073580656
1106	56-57	0.23836950515246969
1106	58-59	0.23062530778327783
1106	60-61	0.18462523715079016
1106	62-63	0.15318377459060883
1106	64-65	0.19292423730778552
1106	66-67	0.18523256484293427
1106	68-69	0.21411196306873137
1106	70-71	0.16487884857770752
1106	72-73	0.19776046713042916
1106	74-75	0.22003100431346212
1106	76-77	0.149329461495455
1106	78-79	0.29152058151795757
1106	80-81	0.2960356884186979
1106	82-83	0.27840049011022217
1106	84-85	0.3113419939313289
1106	86-87	0.33796524326714206
1106	88-89	0.3596699033821409
1106	90-91	0.31288679514531026
1106	92-93	0.29127144573611474
1106	94-95	0.279211300558849
1106	96-97	0.28883268427649256
1106	98-99	0.3272432409110877
1106	100	0.33542579468961264
1107	1	0.1642239618178749
1107	2	0.1453842109343384
1107	3	0.13571780141009526
1107	4	0.09020791772184822
1107	5	0.10800116966179019
1107	6	0.0984427317707457
1107	7	0.10746296187792126
1107	8	0.11625527901215804
1107	9	0.16127096067094016
1107	10-11	0.15420497897614638
1107	12-13	0.1293755362057567
1107	14-15	0.19174722182388138
1107	16-17	0.17564002513583432
1107	18-19	0.19715734297691512
1107	20-21	0.17891024253325583
1107	22-23	0.18527119843304973
1107	24-25	0.17017378540433725
1107	26-27	0.17389983855425584
1107	28-29	0.19342775956359048
1107	30-31	0.18694370100552504
1107	32-33	0.1826884130623938
1107	34-35	0.2019721310468583
1107	36-37	0.20464974039445138
1107	38-39	0.17743706755398847
1107	40-41	0.19060570792757403
1107	42-43	0.15148800722993627
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2314	4	-0.24195346233481985
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2314	82-83	-0.3282610121507332
2314	84-85	-0.4157780274160956
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2314	90-91	-0.38137286821045535
2314	92-93	-0.3309176406828058
2314	94-95	-0.30135337763739756
2314	96-97	-0.3407977894646379
2314	98-99	-0.3624586233006397
2314	100	-0.35660876149449194
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2315	3	-0.4379727957550088
2315	4	-0.2805843318518697
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2315	6	-0.277905415902417
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2315	10-11	-0.33634191350036957
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2315	14-15	-0.41262732785965994
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2315	20-21	-0.44443969794414073
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2315	24-25	-0.42605096377884166
2315	26-27	-0.4619394822422507
2315	28-29	-0.5044549592524277
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2315	96-97	-0.3593933117155075
2315	98-99	-0.3477190670824726
2315	100	-0.4075543424514194
2316	1	-1.6087261454625086
2316	2	-0.8525484734370892
2316	3	-0.6064848391601529
2316	4	-0.357057880649009
2316	5	-0.34635412463433823
2316	6	-0.34030191946803257
2316	7	-0.34963046192049774
2316	8	-0.3441115737586884
2316	9	-0.4167212247105567
2316	10-11	-0.42568439711536854
2316	12-13	-0.4191643319649856
2316	14-15	-0.5565955900030701
2316	16-17	-0.5776649573862329
2316	18-19	-0.5151934087386607
2316	20-21	-0.5349140281703129
2316	22-23	-0.5575312666220142
2316	24-25	-0.5689794421199323
2316	26-27	-0.5815530026072722
2316	28-29	-0.6081316986202694
2316	30-31	-0.6273345947812672
2316	32-33	-0.6269534457198844
2316	34-35	-0.608264830737717
2316	36-37	-0.6074349536471502
2316	38-39	-0.6588429409368644
2316	40-41	-0.6407602267206371
2316	42-43	-0.657939900027408
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2316	46-47	-0.6081601749453469
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2316	50-51	-0.5853125647729698
2316	52-53	-0.5115106563094471
2316	54-55	-0.4763789129091265
2316	56-57	-0.4869258388069966
2316	58-59	-0.485092057921257
2316	60-61	-0.4453262088411165
2316	62-63	-0.39555892419292604
2316	64-65	-0.3872061989582605
2316	66-67	-0.42535026327374226
2316	68-69	-0.44829314073428606
2316	70-71	-0.456064712444082
2316	72-73	-0.4915411925680715
2316	74-75	-0.48512357773085313
2316	76-77	-0.5455847727815879
2316	78-79	-0.7233309096282099
2316	80-81	-0.6912926202247718
2316	82-83	-0.6539462730731742
2316	84-85	-0.6371044351819606
2316	86-87	-0.6555043566207956
2316	88-89	-0.7184557115056478
2316	90-91	-0.6204350491061703
2316	92-93	-0.5472357071018905
2316	94-95	-0.5252300322124199
2316	96-97	-0.5964394682171488
2316	98-99	-1.3946950389248087
2316	100	-0.9673242411063505
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	30.0
7	522.0
8	921.0
9	296.0
10	113.0
11	103.0
12	176.0
13	815.0
14	3526.0
15	11278.0
16	24756.0
17	36135.0
18	42084.0
19	49394.0
20	62295.0
21	85897.0
22	127840.0
23	208249.0
24	334676.0
25	443839.0
26	471445.0
27	432922.0
28	429602.0
29	483496.0
30	577359.0
31	705827.0
32	873849.0
33	1133638.0
34	1598028.0
35	2654233.0
36	5248238.0
37	1.2203302E7
38	1.6493201E7
39	4214550.0
40	5146.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.654377201356	30.52850701575321	28.060843326636554	23.75627245625424
2	22.300168072613648	30.31795371347593	27.368907285991956	20.012970927918467
3	23.285318017170752	26.22604770424542	27.13481233963606	23.353821938947764
4	20.610919845407206	27.93637644647334	25.916554510507737	25.536149197611714
5	22.57409909979376	29.334350345658027	26.774115844833734	21.317434709714476
6	25.454576873081724	27.241391925013918	26.36939978958605	20.9346314123183
7	22.13148917023016	26.616081721514302	29.257563777247174	21.994865331008366
8	24.53290519846069	26.966857423542134	26.497028123067913	22.003209254929263
9	24.060806595789135	25.989772698235647	27.717320358126525	22.23210034784869
10-11	21.754639982559667	28.40954638038027	27.97527424700887	21.860539390051194
12-13	21.72315162731742	27.079076561905453	27.87101400694611	23.32675780383102
14-15	21.706892720485023	29.828810051664718	26.52492256542428	21.93937466242598
16-17	23.213745124600315	26.882392811063067	26.575584583786586	23.328277480550028
18-19	21.782681006632142	28.28605496516518	26.582783037723274	23.348480990479402
20-21	23.200431434312684	28.342129150270846	26.542946384692456	21.914493030724017
22-23	23.16944951986061	26.99233757268245	27.91417783185881	21.924035075598127
24-25	21.909928526209946	28.172870010403127	26.414999436142296	23.502202027244632
26-27	21.96430129727002	26.833385231869205	29.24586640622345	21.956447064637334
28-29	21.996668441951975	26.89889999550093	29.182174964350878	21.922256598196217
30-31	21.9478676841322	26.847188204312445	29.19026982324271	22.014674288312644
32-33	22.03798405297712	26.94413925409311	29.03907477157366	21.978801921356116
34-35	22.050038588062378	27.055632687648277	28.95968561182797	21.93464311246137
36-37	22.03202221944084	27.021739076700293	28.98220797828554	21.96403072557333
38-39	22.096243500569507	27.03864002386703	28.975272358935666	21.889844116627796
40-41	22.115985106492623	27.113242740339576	28.948142338685972	21.82262981448183
42-43	22.15359949688442	27.14033610999716	28.85981760977462	21.8462467833438
44-45	22.202866533568987	27.232064819719277	28.777055294779785	21.788013351931955
46-47	22.24711451513103	27.351994551434373	28.65206173917418	21.748829194260416
48-49	22.294620638230228	27.3921498677855	28.514980522141936	21.798248971842334
50-51	22.34379825701565	27.471034235102216	28.401364760434266	21.783802747447865
52-53	22.414306374821543	27.608004841201378	28.18926403877456	21.788424745202523
54-55	22.561829250985944	27.746817028686927	27.758878803952854	21.932474916374282
56-57	22.780692346226317	28.035936969420572	27.203053974178015	21.980316710175096
58-59	22.855875648143865	28.271271529435953	26.879033316437067	21.993819505983115
60-61	22.86094958792376	28.371761789826692	26.69038828881899	22.07690033343055
62-63	22.825809564864024	28.44624915222869	26.60239817973185	22.125543103175435
64-65	22.833030193258402	28.517378021487232	26.493799335085182	22.15579245016918
66-67	22.76723722785765	28.437841646171325	26.512005077909112	22.282916048061914
68-69	22.70403646798192	28.388610910243077	26.53972613240596	22.36762648936904
70-71	22.629667293000686	28.34374729498062	26.57185313698661	22.45473227503208
72-73	22.561883479172685	28.274352610989585	26.544804685057695	22.618959224780035
74-75	22.52661411135905	28.24789405665552	26.50093393465129	22.724557897334147
76-77	22.502482202126092	28.24265850277539	26.4329333121668	22.821925982931717
78-79	22.4819094857219	28.237272676045848	26.293152346871633	22.987665491360616
80-81	22.440730824881705	28.23101025650684	26.21573083815924	23.112528080452215
82-83	22.39552182276596	28.212033093963512	26.129409751315265	23.263035331955262
84-85	22.323988677467348	28.10398888908362	26.11237645112896	23.459645982320072
86-87	22.28346766381794	28.002870237077666	26.124513134570126	23.589148964534264
88-89	22.24607290049229	27.895818872844703	26.14659278665532	23.711515440007684
90-91	22.196447128477665	27.714950156951602	26.216637635599593	23.87196507897114
92-93	22.149499712442022	27.563682780705562	26.315717926203973	23.97109958064845
94-95	22.107592027780818	27.439015086344643	26.394813886087032	24.058578999787507
96-97	22.04898090885613	27.290443312228184	26.480810224012817	24.179765554902865
98-99	22.013415643607132	27.207591289821043	26.544482177254892	24.234510889316933
100	21.950281453905276	27.19767659945198	26.546707589224162	24.30533435741858
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	99900.0
1	61918.5
2	18370.0
3	11669.0
4	10302.5
5	9890.5
6	9860.5
7	10206.0
8	10108.5
9	9890.0
10	10593.5
11	15350.5
12	115358.5
13	133690.0
14	54349.0
15	56150.5
16	63688.0
17	72140.0
18	80646.5
19	89702.0
20	103757.0
21	125290.0
22	155230.5
23	191035.5
24	228258.0
25	271766.0
26	322318.5
27	379955.0
28	454955.5
29	534568.5
30	603809.0
31	678105.5
32	760612.5
33	837227.5
34	911306.5
35	1005426.0
36	1112906.0
37	1192625.0
38	1264648.5
39	1339303.0
40	1398683.5
41	1466468.5
42	1543667.0
43	1595486.5
44	1636543.0
45	1695269.0
46	1746263.0
47	1784693.5
48	1815963.5
49	1844297.5
50	1873058.5
51	1886444.5
52	1866436.5
53	1806105.5
54	1716962.5
55	1605873.5
56	1472684.5
57	1322656.0
58	1158078.5
59	982081.0
60	804584.0
61	642427.0
62	500990.5
63	375695.5
64	274987.0
65	200681.0
66	144822.0
67	105263.0
68	77317.5
69	58171.5
70	45092.5
71	35971.5
72	29131.5
73	22132.0
74	16877.5
75	13851.5
76	10210.5
77	5921.5
78	3375.5
79	2182.5
80	1314.5
81	853.5
82	537.0
83	279.0
84	171.5
85	106.0
86	67.0
87	45.0
88	23.5
89	13.5
90	9.0
91	6.0
92	6.5
93	2.5
94	1.5
95	3.0
96	1.5
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1076376398677056
2	0.07367368222836733
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.165993164600332E-4
20-21	0.002209046198396941
22-23	7.83327986209179E-4
24-25	8.04775036679052E-4
26-27	0.0067098629327174775
28-29	0.006553605850722687
30-31	0.007738300067153779
32-33	0.007794470913622495
34-35	0.007193953500466045
36-37	0.010895101638695594
38-39	0.01341768328919973
40-41	0.016878828719790222
42-43	0.023637713482153124
44-45	0.011773409419842782
46-47	0.01862523140090847
48-49	0.016276268730398544
50-51	0.018921404955016242
52-53	0.021296921116584103
54-55	0.11307906295834771
56-57	0.10607506904775771
58-59	0.10320218557291229
60-61	0.1459196036683117
62-63	0.09637895965913977
64-65	0.07512084749102498
66-67	0.08493951145375644
68-69	0.07793653883128404
70-71	0.05464810588535456
72-73	0.03227474709280635
74-75	0.021035471358475173
76-77	0.012843719367101216
78-79	0.009053719162639336
80-81	0.005937769115802041
82-83	5.566020240990906E-4
84-85	0.0025389222603859436
86-87	0.0017351685118245045
88-89	0.002250919011219075
90-91	0.0032803774337729894
92-93	0.004975715709010586
94-95	0.002295855688394047
96-97	0.0012347373341941295
98-99	0.00169635956335521
100	0.001877127560172713
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4.8957781E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.36812778381754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52836917781377	33.95420462768406
2	10.847957060899342	9.626070901426955
3	4.121461807852659	5.4858463244079125
4	2.1728097180646158	3.8561399688404574
5	1.3710769043809832	3.0416057647508223
6	0.9349983161647366	2.4890474861550755
7	0.649941084005405	2.018566834697329
8	0.482035161604436	1.7109598117087004
9	0.3918880393145079	1.564860474473022
>10	2.207773881870973	17.795210053860185
>50	0.17041747296543505	5.241736544027454
>100	0.11976417698528348	9.85990027419857
>500	0.0011695388236120025	0.32417890869933014
>1k	2.9173760970841324E-4	0.22921101870196106
>5k	2.7553059567364644E-5	0.07117871573777546
>10k+	1.8368584894178586E-5	2.731282290630354
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1182238	2.4148112431811404	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66866	0.1365789025446231	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55820	0.11401660545031647	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.007537106307984016	1.0212881176130103E-5	0.0	4.085152470452041E-6	2.0425762352260206E-6
2	0.00754527661292492	2.0425762352260206E-5	0.0	4.085152470452041E-6	8.170304940904082E-6
3	0.00754527661292492	2.4510914822712247E-5	0.0	4.085152470452041E-6	1.2255457411356124E-5
4	0.007547319189160146	3.268121976361633E-5	0.0	6.127728705678062E-6	2.0425762352260206E-5
5	0.007549361765395372	3.880894846929439E-5	0.0	6.127728705678062E-6	3.0638643528390306E-5
6	0.007549361765395372	4.697925341019847E-5	0.0	8.170304940904082E-6	3.676637223406837E-5
7	0.007551404341630598	5.106440588065051E-5	0.0	1.0212881176130103E-5	4.085152470452041E-5
8	0.00755548949410105	5.514955835110255E-5	0.0	1.4298033646582144E-5	4.289410093974643E-5
9	0.00755548949410105	5.719213458632858E-5	0.0	1.6340609881808165E-5	4.697925341019847E-5
10-11	0.0075636597990419545	7.353274446813674E-5	0.0	1.9404474234647197E-5	5.4128270233489544E-5
12-13	0.007582042985158988	8.170304940904082E-5	0.0	2.6553491057938268E-5	5.8213422703941585E-5
14-15	0.007598383595040796	9.804365929084899E-5	0.0	2.9617355410777297E-5	6.331986329200664E-5
16-17	0.007611660340569766	1.0825654046697909E-4	0.0	3.778766035168138E-5	7.149016823291071E-5
18-19	0.007646384136568608	1.2561843846640026E-4	0.0	4.187281282213342E-5	7.761789693858879E-5
20-21	0.007691320813743581	1.5012935328911252E-4	0.0	4.289410093974643E-5	8.783077811471888E-5
22-23	0.00791191904714799	1.6238481070046863E-4	0.0	4.595796529258546E-5	1.0621396423175307E-4
24-25	0.009178316312988124	1.685125394061467E-4	0.0	4.697925341019847E-5	1.194907097607222E-4
26-27	0.014417524356342866	1.777041324646638E-4	0.0	4.697925341019847E-5	1.297035909368523E-4
28-29	0.03010655241911393	1.98129894816924E-4	0.0	5.106440588065051E-5	1.3583131964253037E-4
30-31	0.06696075543129702	2.0834277599305408E-4	0.0	5.3106982115876535E-5	1.4298033646582145E-4
32-33	0.13758180747611906	2.2264080963963623E-4	0.0	5.719213458632858E-5	1.491080651714995E-4
34-35	0.2536624362121314	2.4000270763905741E-4	0.0	5.719213458632858E-5	1.6136352258285563E-4
36-37	0.42448615062843637	2.5327945316802657E-4	2.0425762352260206E-6	5.719213458632858E-5	1.7259769187659874E-4
38-39	0.6543372543784205	2.594071818737046E-4	2.0425762352260206E-6	5.719213458632858E-5	1.8281057305272885E-4
40-41	0.9464685501166812	2.6655619869699564E-4	2.0425762352260206E-6	6.127728705678061E-5	1.8791701364079388E-4
42-43	1.2986679277804685	2.788116561083518E-4	2.0425762352260206E-6	6.229857517439362E-5	1.9506603046408495E-4
44-45	1.710847556591668	2.9004582540209493E-4	3.063864352839031E-6	6.331986329200664E-5	2.0017247105215E-4
46-47	2.1856668708085443	3.0638643528390307E-4	4.085152470452041E-6	6.94475919976847E-5	2.063001997578281E-4
48-49	2.720871887555525	3.257909095185503E-4	4.085152470452041E-6	7.353274446813674E-5	2.1957694528679722E-4
50-51	3.313429381123299	3.400889431651324E-4	8.170304940904082E-6	7.455403258574975E-5	2.3285369081576634E-4
52-53	3.956207696586575	3.594934173997796E-4	8.170304940904082E-6	7.557532070336276E-5	2.5225816505041355E-4
54-55	4.643225149440495	3.707275866935227E-4	8.170304940904082E-6	7.761789693858878E-5	2.634923343441567E-4
56-57	5.37335525889133	3.8298304410487886E-4	8.170304940904082E-6	8.170304940904082E-5	2.696200630498347E-4
58-59	6.148331150874669	3.99323653986687E-4	8.170304940904082E-6	8.88520662323319E-5	2.8085423234357784E-4
60-61	6.960231714750307	4.1055782328043014E-4	8.170304940904082E-6	9.497979493800995E-5	2.8391809669641685E-4
62-63	7.805390526176012	4.2179199257417327E-4	8.170304940904082E-6	1.00086235526075E-4	2.9106711351970795E-4
64-65	8.675581109364414	4.340474499855294E-4	8.170304940904082E-6	1.092778285845921E-4	2.97194842225386E-4
66-67	9.570315288595289	4.432390430440464E-4	8.170304940904082E-6	1.205119978783352E-4	3.0332257093106406E-4
68-69	10.491931813657976	4.5038805986733757E-4	8.170304940904082E-6	1.2357586223117424E-4	3.2374833328332423E-4
70-71	11.432600468554732	4.5957965292585466E-4	8.170304940904082E-6	1.2561843846640026E-4	3.370250788122934E-4
72-73	12.391355114726299	4.7285639845482373E-4	8.170304940904082E-6	1.297035909368523E-4	3.4417409563558446E-4
74-75	13.364448646069151	4.8409056774856686E-4	8.170304940904082E-6	1.3174616717207834E-4	3.523444005764885E-4
76-77	14.350983350327908	4.912395845718579E-4	8.170304940904082E-6	1.3378874340730433E-4	3.574508411645536E-4
78-79	15.35238903086723	4.973673132775359E-4	8.170304940904082E-6	1.4195904834820843E-4	3.605147055173926E-4
80-81	16.365344458728636	5.034950419832141E-4	8.170304940904082E-6	1.5115064140672552E-4	3.697062985759097E-4
82-83	17.388223743228885	5.147292112769572E-4	8.170304940904082E-6	1.582996582300166E-4	3.748127391639748E-4
84-85	18.415708015851454	5.269846686883134E-4	8.170304940904082E-6	1.6340609881808165E-4	3.809404678696528E-4
86-87	19.447922895034807	5.341336855116044E-4	8.170304940904082E-6	1.685125394061467E-4	3.860469084577179E-4
88	20.228437232479962	5.392401260996694E-4	8.170304940904082E-6	1.695338275237597E-4	3.9830236586907403E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	164285	0.0	79.02011	8
GTGGTAT	168325	0.0	77.24075	6
GTATCAA	168495	0.0	76.86171	9
AGTGGTA	170430	0.0	76.38598	5
TGGTATC	170105	0.0	76.316505	7
AAGCAGT	181125	0.0	72.45213	1
GCAGTGG	184515	0.0	70.93439	3
CAGTGGT	187935	0.0	69.47606	4
AGCAGTG	196855	0.0	66.77979	2
CAACGCA	158615	0.0	43.056534	12-13
ATCAACG	161795	0.0	42.31899	10-11
ACGCAGA	165335	0.0	41.287968	14-15
TCAACGC	157490	0.0	40.937496	12-13
AGAGTAC	167960	0.0	40.330044	18-19
AACGCAG	161475	0.0	39.791775	14-15
GAGTACT	158930	0.0	38.909637	20-21
CGCAGAG	167990	0.0	38.189476	16-17
TATCAAC	171555	0.0	37.739895	10-11
AGTACTT	175255	0.0	37.535854	20-21
GTACTTT	169420	0.0	36.468163	22-23
>>END_MODULE