FastQCFastQC Report
Thu 26 May 2016
SRR1660670_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660670_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28221068
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15034015.327229288416724No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1526540.5409221224370389Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA753230.2669034354050669No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT434410.15393109856792095No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318890.11299714100118394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1918750.084.517268
GTGGTAT1929100.084.110836
AGTGGTA1937900.083.879025
TGGTATC1939800.083.502647
GTATCAA1937900.083.4804159
AAGCAGT1969100.083.389961
GCAGTGG2040950.080.4270553
CAGTGGT2043550.079.809284
AGCAGTG2113550.078.066542
CAACGCA1901200.044.9595612-13
ATCAACG1918750.044.57080510-11
ACGCAGA1935950.044.1566914-15
AGAGTAC1935200.043.6531618-19
TCAACGC1892800.042.79842412-13
AGTACTT1925600.042.5565320-21
AACGCAG1918800.042.14906314-15
GAGTACT1854250.041.82211720-21
TATCAAC1953850.041.401310-11
CGCAGAG1958800.041.22711616-17
GCAGAGT2083850.040.80058316-17
Produced by FastQC (version 0.11.5)