##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660670_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28221068 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.416883372379814 34.0 31.0 34.0 31.0 34.0 2 32.56658001036672 34.0 31.0 34.0 31.0 34.0 3 32.58504685223111 34.0 31.0 34.0 31.0 34.0 4 35.94707829625725 37.0 37.0 37.0 35.0 37.0 5 35.94134786110859 37.0 37.0 37.0 35.0 37.0 6 35.95483959005379 37.0 37.0 37.0 35.0 37.0 7 35.886834651332116 37.0 36.0 37.0 35.0 37.0 8 35.91960144810962 37.0 37.0 37.0 35.0 37.0 9 37.65740478000337 39.0 38.0 39.0 35.0 39.0 10-11 37.56279891675254 39.0 38.0 39.0 35.0 39.0 12-13 37.64797466205035 39.0 38.0 39.0 35.0 39.0 14-15 39.13686912912013 41.0 39.0 41.0 36.0 41.0 16-17 39.09868671518739 41.0 39.0 41.0 36.0 41.0 18-19 39.03720094859628 41.0 39.0 41.0 36.0 41.0 20-21 38.94945722819561 40.5 39.0 41.0 35.5 41.0 22-23 38.799975181662155 40.0 38.5 41.0 34.5 41.0 24-25 38.80851805466753 40.0 39.0 41.0 35.0 41.0 26-27 38.80435843179287 40.0 39.0 41.0 35.0 41.0 28-29 38.7312211749038 40.0 38.5 41.0 35.0 41.0 30-31 38.61680091270819 40.0 38.0 41.0 34.5 41.0 32-33 38.514218774427675 40.0 38.0 41.0 34.0 41.0 34-35 38.43016536440081 40.0 38.0 41.0 34.0 41.0 36-37 38.26526717557252 40.0 38.0 41.0 34.0 41.0 38-39 37.85118610677668 40.0 37.0 41.0 33.0 41.0 40-41 37.716310240278645 40.0 37.0 41.0 33.0 41.0 42-43 37.53484315334912 40.0 36.0 41.0 33.0 41.0 44-45 37.40733855288538 40.0 36.0 41.0 33.0 41.0 46-47 37.205499717445136 40.0 35.0 41.0 32.0 41.0 48-49 37.244886338107406 40.0 35.0 41.0 32.0 41.0 50-51 36.27384201051498 38.5 34.5 40.0 31.0 40.5 52-53 36.39924491872526 38.5 35.0 39.5 31.0 40.5 54-55 36.73304087924667 40.0 35.0 41.0 30.5 41.0 56-57 36.38047489910729 40.0 35.0 41.0 28.5 41.0 58-59 36.15889301212839 39.0 35.0 41.0 28.0 41.0 60-61 35.961071902027236 39.0 35.0 41.0 28.0 41.0 62-63 35.71852344496672 38.5 35.0 41.0 28.0 41.0 64-65 35.42143468843915 37.5 35.0 40.5 27.0 41.0 66-67 35.04631582334163 37.0 35.0 40.0 26.0 41.0 68-69 34.704868770381054 36.5 34.5 39.5 26.0 41.0 70-71 34.35724679165225 36.0 34.0 39.0 26.0 41.0 72-73 33.9900815234916 35.5 34.0 39.0 26.0 41.0 74-75 33.61618018850314 35.0 34.0 37.5 25.0 40.0 76-77 33.24286136158986 35.0 34.0 37.0 24.5 39.0 78-79 32.88742644325154 35.0 33.5 36.5 24.0 39.0 80-81 32.56446968980763 35.0 33.0 36.0 23.5 38.5 82-83 32.28948388841981 35.0 33.0 36.0 23.0 37.0 84-85 32.044768309264555 35.0 33.0 35.0 21.5 37.0 86-87 31.836501120368656 35.0 33.0 35.0 20.0 36.5 88-89 31.642521909518095 35.0 33.0 35.0 20.0 36.0 90-91 31.500662997587476 35.0 33.0 35.0 20.0 36.0 92-93 31.36357362520795 35.0 33.0 35.0 19.0 36.0 94-95 31.250741077552416 35.0 32.5 35.0 19.0 35.5 96-97 31.142803171021026 35.0 32.0 35.0 18.5 35.0 98-99 30.894409913898368 35.0 32.0 35.0 17.0 35.0 100 30.819227925746823 35.0 32.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49433007766401715 1101 2 0.47392774071828825 1101 3 0.46210648299648227 1101 4 0.41380443716877835 1101 5 0.394642845847315 1101 6 0.3536622788536832 1101 7 0.3904312526422302 1101 8 0.3910992009485952 1101 9 0.48300905937711747 1101 10-11 0.48230764706113405 1101 12-13 0.45556695831667327 1101 14-15 0.5796287733436074 1101 16-17 0.5957810612850594 1101 18-19 0.5801448612009992 1101 20-21 0.6140572310918131 1101 22-23 0.6321167014355638 1101 24-25 0.6480374411386123 1101 26-27 0.5889963388311656 1101 28-29 0.6105753387058712 1101 30-31 0.6194141366352781 1101 32-33 0.6184066695371868 1101 34-35 0.5913147575019195 1101 36-37 0.6062769421574785 1101 38-39 0.6591071526086552 1101 40-41 0.6657119802173099 1101 42-43 0.6546365537084284 1101 44-45 0.6447313036784905 1101 46-47 0.6229528339783457 1101 48-49 0.6359754416108103 1101 50-51 0.5772863686782586 1101 52-53 0.6581449300379134 1101 54-55 0.6955266691451101 1101 56-57 0.6838957449765317 1101 58-59 0.6122851122783644 1101 60-61 0.6065762037231721 1101 62-63 0.5814770231190849 1101 64-65 0.5676888354474698 1101 66-67 0.556941295580728 1101 68-69 0.5350818163315552 1101 70-71 0.5598189812255114 1101 72-73 0.5272397241992408 1101 74-75 0.5039365205710311 1101 76-77 0.4835016365529725 1101 78-79 0.4786821727279218 1101 80-81 0.4513394701539468 1101 82-83 0.4403960011544399 1101 84-85 0.4678113712953902 1101 86-87 0.45734807706034175 1101 88-89 0.44203048609039186 1101 90-91 0.41265167725392615 1101 92-93 0.41032597514247016 1101 94-95 0.4468831510225151 1101 96-97 0.4647004918583022 1101 98-99 0.3121693814983004 1101 100 0.3514714161124708 1102 1 0.47172008318881353 1102 2 0.4242889428928933 1102 3 0.4276084731054013 1102 4 0.369219471123138 1102 5 0.35598636845303844 1102 6 0.30158738631985926 1102 7 0.3428567485499272 1102 8 0.36358831808997394 1102 9 0.4245920171535644 1102 10-11 0.46692318130021704 1102 12-13 0.4367925507251371 1102 14-15 0.5290791559419716 1102 16-17 0.5463895670680046 1102 18-19 0.5389395303172719 1102 20-21 0.5006406304109206 1102 22-23 0.5339974160592647 1102 24-25 0.5284562829999615 1102 26-27 0.5411281067005689 1102 28-29 0.5350109869278654 1102 30-31 0.5048035354034255 1102 32-33 0.5180407247898984 1102 34-35 0.5038633727914217 1102 36-37 0.5203061149527812 1102 38-39 0.5336898738888181 1102 40-41 0.5224992392976233 1102 42-43 0.5740373488029604 1102 44-45 0.5115710630122905 1102 46-47 0.5455392101802801 1102 48-49 0.5569144691209615 1102 50-51 0.5374386375057014 1102 52-53 0.5718308011921778 1102 54-55 0.589867909807765 1102 56-57 0.5981516122235462 1102 58-59 0.575581200398517 1102 60-61 0.5709922706333899 1102 62-63 0.5933693060664851 1102 64-65 0.5623489275026117 1102 66-67 0.5075788609225356 1102 68-69 0.507759578642883 1102 70-71 0.5050623031109325 1102 72-73 0.48389871171312393 1102 74-75 0.4796748273021123 1102 76-77 0.45572127000117746 1102 78-79 0.4211695315376147 1102 80-81 0.4478123015254454 1102 82-83 0.3987436842323504 1102 84-85 0.4192000848963531 1102 86-87 0.422354812976824 1102 88-89 0.45306072871602154 1102 90-91 0.4270289825819269 1102 92-93 0.42074531587542907 1102 94-95 0.40573706075083393 1102 96-97 0.42386068346304384 1102 98-99 0.25677139676611205 1102 100 0.30578349123348403 1103 1 0.38717838268072313 1103 2 0.38930763469956275 1103 3 0.3810972447034615 1103 4 0.3551308768723729 1103 5 0.3647653092294618 1103 6 0.3090684929301091 1103 7 0.33250864268526925 1103 8 0.3475926171033592 1103 9 0.42289636309766365 1103 10-11 0.43414524157496004 1103 12-13 0.3858135615153131 1103 14-15 0.4774223444587449 1103 16-17 0.5048180816978913 1103 18-19 0.5120750682124395 1103 20-21 0.521754732506416 1103 22-23 0.5225481252641515 1103 24-25 0.5405747121958555 1103 26-27 0.516282231596378 1103 28-29 0.5420475520079222 1103 30-31 0.531751857597321 1103 32-33 0.55364739496639 1103 34-35 0.5436056284639932 1103 36-37 0.500664904959379 1103 38-39 0.5295157856901938 1103 40-41 0.5456337365792052 1103 42-43 0.580900491333395 1103 44-45 0.5608277899633336 1103 46-47 0.5690341177706415 1103 48-49 0.5154142046810009 1103 50-51 0.4836501453286175 1103 52-53 0.5542500509624873 1103 54-55 0.6181260475444148 1103 56-57 0.5866562948032836 1103 58-59 0.5499536707886179 1103 60-61 0.5410346094767604 1103 62-63 0.4791975362403562 1103 64-65 0.4870802931063025 1103 66-67 0.48016464361679567 1103 68-69 0.49803150949298924 1103 70-71 0.44887227718017186 1103 72-73 0.42255929252787183 1103 74-75 0.41712828299557003 1103 76-77 0.3832000273069269 1103 78-79 0.36165059516766007 1103 80-81 0.3702649859237468 1103 82-83 0.3418598096243386 1103 84-85 0.37360589673657785 1103 86-87 0.37255046169599737 1103 88-89 0.3701245699010478 1103 90-91 0.3600911138765426 1103 92-93 0.36992503264790955 1103 94-95 0.3819415027120634 1103 96-97 0.40997072802479195 1103 98-99 0.28029674469049226 1103 100 0.33255531104343206 1104 1 0.36207614673780597 1104 2 0.3482150402776796 1104 3 0.3362008114432271 1104 4 0.2900023974869512 1104 5 0.30323057930638697 1104 6 0.28401553591601925 1104 7 0.282763172618985 1104 8 0.29382594867703204 1104 9 0.3579479343429526 1104 10-11 0.3894062809292578 1104 12-13 0.3444957709211849 1104 14-15 0.44371867640832363 1104 16-17 0.43448482668210886 1104 18-19 0.4461202051095299 1104 20-21 0.46114564005028313 1104 22-23 0.4409062355617266 1104 24-25 0.46092620971717935 1104 26-27 0.4220463094309679 1104 28-29 0.439266299200753 1104 30-31 0.41700554134063594 1104 32-33 0.44152594400393497 1104 34-35 0.4551560726024064 1104 36-37 0.42599510770293847 1104 38-39 0.4770576997761111 1104 40-41 0.45540859537892686 1104 42-43 0.481189367897187 1104 44-45 0.45117449751122507 1104 46-47 0.46536932020633515 1104 48-49 0.42481384842917436 1104 50-51 0.33806816180851484 1104 52-53 0.4391913230123592 1104 54-55 0.5152319710982525 1104 56-57 0.4682261460693127 1104 58-59 0.45474879276774516 1104 60-61 0.4548185707086887 1104 62-63 0.4951347988765704 1104 64-65 0.47625903269090486 1104 66-67 0.4268968876270236 1104 68-69 0.4310115892798052 1104 70-71 0.4159451089471702 1104 72-73 0.3841898205207528 1104 74-75 0.369864362985048 1104 76-77 0.3681094708757797 1104 78-79 0.3835062162428855 1104 80-81 0.3960127576503254 1104 82-83 0.3901424942060956 1104 84-85 0.3708785342777361 1104 86-87 0.3546248509317316 1104 88-89 0.3398036300015086 1104 90-91 0.39979555808058365 1104 92-93 0.3808330609757249 1104 94-95 0.3819813556294953 1104 96-97 0.38591425655443956 1104 98-99 0.25730676232879546 1104 100 0.3426768575283994 1105 1 0.30704953057222184 1105 2 0.2901560236307219 1105 3 0.2902093043905296 1105 4 0.2604395713048362 1105 5 0.28141417894400433 1105 6 0.2692041336042692 1105 7 0.27657539674999043 1105 8 0.2782052449034964 1105 9 0.323626551058247 1105 10-11 0.331604140103849 1105 12-13 0.3322353943452043 1105 14-15 0.41885521841253137 1105 16-17 0.40387970635185155 1105 18-19 0.4296783315286987 1105 20-21 0.40513195829289117 1105 22-23 0.43337311523718114 1105 24-25 0.4206771545240784 1105 26-27 0.42635046935228615 1105 28-29 0.4266262501768452 1105 30-31 0.4153451254208278 1105 32-33 0.3991016834044032 1105 34-35 0.40098441027914333 1105 36-37 0.4042951049930892 1105 38-39 0.43957316682810443 1105 40-41 0.4267201810133088 1105 42-43 0.4338381839609582 1105 44-45 0.42214346744172104 1105 46-47 0.4095023054762379 1105 48-49 0.4230423068472291 1105 50-51 0.3578918516781471 1105 52-53 0.4250614655353928 1105 54-55 0.4483844000290276 1105 56-57 0.4346979490197356 1105 58-59 0.4510748967505478 1105 60-61 0.4020046496589629 1105 62-63 0.420271968680602 1105 64-65 0.41587506828248877 1105 66-67 0.3960874649349364 1105 68-69 0.3750778787775886 1105 70-71 0.35938581438736605 1105 72-73 0.3609393177969977 1105 74-75 0.35870031209154263 1105 76-77 0.3563909059207333 1105 78-79 0.3652199355511101 1105 80-81 0.34152348974182445 1105 82-83 0.3293767880298617 1105 84-85 0.37247826500382075 1105 86-87 0.3877791123248535 1105 88-89 0.3781198831047199 1105 90-91 0.3707616245960885 1105 92-93 0.3735147678923809 1105 94-95 0.37176211163189876 1105 96-97 0.3614641595345347 1105 98-99 0.2279955718135298 1105 100 0.23489335713664516 1106 1 0.3364022389261976 1106 2 0.3091199211724316 1106 3 0.3256158192411931 1106 4 0.2698104333554383 1106 5 0.28994035647795613 1106 6 0.28293925585043667 1106 7 0.28079064829008615 1106 8 0.2846315245410338 1106 9 0.33585524800810873 1106 10-11 0.35790168604290784 1106 12-13 0.3367294530502605 1106 14-15 0.3828110812400496 1106 16-17 0.42066179153999883 1106 18-19 0.43445880988102914 1106 20-21 0.45414565652001215 1106 22-23 0.4446909081048318 1106 24-25 0.4181819209547868 1106 26-27 0.4240463488908688 1106 28-29 0.4655434355068664 1106 30-31 0.4529015644851526 1106 32-33 0.47462101323624495 1106 34-35 0.4775750180573226 1106 36-37 0.48464257480865314 1106 38-39 0.47064377764539245 1106 40-41 0.49691274992481027 1106 42-43 0.5148199598522112 1106 44-45 0.48302646214357736 1106 46-47 0.5403761750995386 1106 48-49 0.5204806389841963 1106 50-51 0.4664815188532003 1106 52-53 0.49859217316526383 1106 54-55 0.502031262031366 1106 56-57 0.49233549266140386 1106 58-59 0.46637223348681545 1106 60-61 0.5043305031553658 1106 62-63 0.46759726615808717 1106 64-65 0.4162932932448058 1106 66-67 0.47223418238037596 1106 68-69 0.44589657603221156 1106 70-71 0.481032674797639 1106 72-73 0.44476670714180244 1106 74-75 0.43879752322319376 1106 76-77 0.4196454096137785 1106 78-79 0.4075111479717748 1106 80-81 0.38416041120688504 1106 82-83 0.37411534495045373 1106 84-85 0.43988638622204235 1106 86-87 0.43532157459069154 1106 88-89 0.4331972445883885 1106 90-91 0.41917894202742545 1106 92-93 0.40601262214901723 1106 94-95 0.4188701473254248 1106 96-97 0.3905500045611454 1106 98-99 0.33052375478838414 1106 100 0.36514131080373247 1107 1 0.2976978932819776 1107 2 0.2486135067311892 1107 3 0.24031865640042582 1107 4 0.25509849644016924 1107 5 0.2611037344257241 1107 6 0.2188167731616275 1107 7 0.2082022328422397 1107 8 0.2573373083833772 1107 9 0.3075383028163259 1107 10-11 0.35206500905258764 1107 12-13 0.3052242881070413 1107 14-15 0.3420351248548741 1107 16-17 0.3665987294592128 1107 18-19 0.3798630303323236 1107 20-21 0.38113667265637474 1107 22-23 0.36846793149011603 1107 24-25 0.35581050937707204 1107 26-27 0.31970437191769463 1107 28-29 0.35260055931841094 1107 30-31 0.38134567466961045 1107 32-33 0.4143016355609319 1107 34-35 0.36716407219275027 1107 36-37 0.36565918712892653 1107 38-39 0.3843069778355712 1107 40-41 0.4040869069854338 1107 42-43 0.3670141418000341 1107 44-45 0.43742315560132994 1107 46-47 0.3910581424793307 1107 48-49 0.347125457340006 1107 50-51 0.23777391401274883 1107 52-53 0.332178386264836 1107 54-55 0.40673731602873886 1107 56-57 0.35394145475930117 1107 58-59 0.4153731614422185 1107 60-61 0.410766034250706 1107 62-63 0.40163675259064036 1107 64-65 0.3502080368438669 1107 66-67 0.36540035685013805 1107 68-69 0.3677117985638034 1107 70-71 0.3010979371707876 1107 72-73 0.32273295030466187 1107 74-75 0.3313610566719589 1107 76-77 0.34251960316882446 1107 78-79 0.3469613457669709 1107 80-81 0.32434300867350174 1107 82-83 0.3550178664620631 1107 84-85 0.3601545236683492 1107 86-87 0.3634436746654792 1107 88-89 0.36589295870900784 1107 90-91 0.36421830269090805 1107 92-93 0.3618040889450782 1107 94-95 0.34943338678843716 1107 96-97 0.3367362051647369 1107 98-99 0.2093603213962929 1107 100 0.2570727419792611 1108 1 0.13805421353527692 1108 2 0.11989952700093909 1108 3 0.11117446235704875 1108 4 0.11978530324731196 1108 5 0.13311815166262164 1108 6 0.10629500842142647 1108 7 0.10155932729364281 1108 8 0.1160007739800335 1108 9 0.13998466467984372 1108 10-11 0.1276675186790328 1108 12-13 0.11156777954439434 1108 14-15 0.1178958161621324 1108 16-17 0.16961941757936927 1108 18-19 0.15928051948156252 1108 20-21 0.18351433751099222 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-0.6376175315877681 2316 92-93 -0.6293827496770774 2316 94-95 -0.5689485649141766 2316 96-97 -0.6296522929961768 2316 98-99 -0.5112657712486097 2316 100 -0.5627184179842786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 791.0 3 272.0 4 234.0 5 213.0 6 777.0 7 6481.0 8 13543.0 9 18047.0 10 20822.0 11 20715.0 12 20789.0 13 22282.0 14 26644.0 15 35263.0 16 46997.0 17 61761.0 18 71261.0 19 79469.0 20 91798.0 21 112213.0 22 148678.0 23 225620.0 24 347335.0 25 471259.0 26 522269.0 27 474527.0 28 348022.0 29 331298.0 30 369379.0 31 444358.0 32 554175.0 33 719296.0 34 1013700.0 35 1668008.0 36 3121568.0 37 6399002.0 38 8066736.0 39 2340663.0 40 4803.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.369059288084834 32.22509388533511 27.75897313310565 23.646873693474397 2 22.36625047174017 33.98739343635483 24.403058347589678 19.24329774431532 3 26.546293997218157 24.597734504808617 26.49909352264747 22.356877975325755 4 19.565649711936466 27.024035235574644 23.66907187109415 29.74124318139474 5 22.490804152093247 31.510717767650558 25.430925709918323 20.56755237033787 6 30.249436871621626 27.02952737147734 23.989781361464036 18.731254395437 7 22.581178192449006 24.624477718951056 31.3806633225459 21.41368076605404 8 28.296303591241024 26.890042285824606 23.923944150990327 20.889709971944047 9 26.891709659504098 25.075787769574553 26.388407409754745 21.644095161166604 10-11 21.66279754942302 29.414469494875917 27.827291140813436 21.095441814887625 12-13 21.37640860778737 26.06403198257156 27.93305585864455 24.626503550996517 14-15 21.348034142642263 32.079511652191194 25.361837476655367 21.210616728511177 16-17 24.57085756294951 25.662306890709957 25.155224861877873 24.611610684462658 18-19 21.360294303317733 28.87067100089691 25.573121440520502 24.195913255264855 20-21 24.13571727669103 29.378425716776775 25.508360035059123 20.977496971473077 22-23 24.6382676114392 26.00795977829294 28.242729822250872 21.111042788016988 24-25 21.374609007429704 29.0278246330343 25.16957113733682 24.427995222199176 26-27 21.12075634935525 26.432871450138112 31.395359757636605 21.051012442870032 28-29 21.525703860088836 26.158000070172477 31.30301610529274 21.013279964445942 30-31 21.397323216623196 25.746554417048035 31.714540278811416 21.141582087517357 32-33 21.50194201394916 25.992325219276157 31.448126691363772 21.057606075410913 34-35 21.5134015563715 26.436900387468043 31.011673725377126 21.038024330783323 36-37 21.08513642579076 26.40105011704989 31.475732291168384 21.03808116599096 38-39 21.157032683991172 26.13261455112932 31.486872975436984 21.22347978944252 40-41 21.50808795983844 26.19373728476711 31.08683190328102 21.21134285211343 42-43 21.51736608939641 26.22478806995876 31.360579443064672 20.897266397580154 44-45 21.87137843693562 26.32058044568648 31.009177436770823 20.798863680607074 46-47 21.33663934578609 26.40916338127367 31.17949677295347 21.074700499986772 48-49 21.654640201816218 26.462700261258533 30.81627368487237 21.06638585205288 50-51 21.799054684671926 26.555853259906353 30.6799972560312 20.96509479939052 52-53 21.666330475403388 26.9859064239514 30.680476221200514 20.667286879444696 54-55 21.967925684785165 27.02244868190553 29.928400758405466 21.08122487490384 56-57 22.439196223333806 27.90869560586354 28.902672007530615 20.74943616327204 58-59 22.649171039752304 28.263164951593406 28.39377182769632 20.69389218095797 60-61 22.755446859552805 28.663307468235537 27.986156677270802 20.59508899494086 62-63 22.879930969493117 28.778948119862243 27.860931558807327 20.480189351837314 64-65 23.068681564950367 28.773580675595344 27.82150465668398 20.33623310277031 66-67 23.16621678220331 28.601085032080032 28.016219871064585 20.216478314652072 68-69 23.36485578419364 28.42986572196547 28.168060185288397 20.037218308552497 70-71 23.559496761065542 28.243788493889056 28.253596706468148 19.943118038577257 72-73 23.736384448963683 28.12811295329944 28.25469508819062 19.88080750954626 74-75 24.020430350830182 28.198473395555375 28.074020212338418 19.707076041276025 76-77 24.340567348147893 28.25986946453995 27.726671269552167 19.67289191775999 78-79 24.611254597742533 28.396347707473396 27.381150275164018 19.61124741962005 80-81 24.88868447652421 28.549366811905507 27.017931761783014 19.544016949787263 82-83 25.11936480557991 28.57387339534061 26.723222209047787 19.58353959003169 84-85 25.280229235807088 28.49545391343094 26.605128755937223 19.619188094824743 86-87 25.497048504489943 28.42957574855261 26.52863717209421 19.54473857486324 88-89 25.69079076099903 28.329822701451263 26.46452051038025 19.51486602716946 90-91 25.838716645965754 28.244105060860967 26.44600230856713 19.47117598460615 92-93 25.957340793264994 28.303430387839633 26.35849608733397 19.3807327315614 94-95 26.040073089210868 28.31335646877409 26.303704464883904 19.34286597713114 96-97 26.05809463680533 28.313915444235356 26.27947897195305 19.348510947006268 98-99 26.141628131459 28.471534342997806 26.11437056390286 19.272466961640337 100 26.13930371775332 28.601381458544388 25.957348698833886 19.301966124868397 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 86594.0 1 56027.5 2 19661.5 3 12706.5 4 11296.0 5 10811.0 6 10947.5 7 11187.5 8 10900.5 9 11113.0 10 11920.0 11 18006.0 12 174462.5 13 203342.0 14 79310.5 15 82376.0 16 91919.0 17 99001.0 18 104112.5 19 109045.5 20 115836.5 21 126821.5 22 142299.0 23 159285.0 24 176383.5 25 196155.5 26 218605.5 27 245532.5 28 279555.0 29 314629.5 30 346756.5 31 381933.0 32 421292.0 33 459023.5 34 494694.5 35 537705.0 36 587355.0 37 627214.5 38 668114.0 39 711271.0 40 742499.5 41 777929.5 42 819659.0 43 844965.5 44 862191.0 45 889320.0 46 919441.5 47 951985.0 48 990000.0 49 1025437.5 50 1047500.5 51 1056255.5 52 1046278.5 53 1014850.0 54 972930.0 55 921382.5 56 860495.0 57 787545.0 58 697683.0 59 593765.0 60 485926.5 61 385681.5 62 298795.0 63 225439.0 64 166382.0 65 120975.0 66 85771.5 67 61648.0 68 44879.5 69 33024.5 70 25216.0 71 19966.5 72 15788.0 73 11823.0 74 9173.0 75 7712.0 76 5783.5 77 3370.5 78 2027.0 79 1540.0 80 1124.5 81 856.0 82 707.5 83 573.5 84 489.5 85 420.5 86 366.0 87 310.5 88 249.0 89 200.5 90 163.5 91 142.0 92 121.0 93 94.5 94 74.5 95 67.0 96 51.5 97 41.0 98 37.5 99 22.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13471850179447498 2 0.05078829759384018 3 0.01860312302851189 4 0.07578026458814387 5 0.07962845346604176 6 0.08075881465577418 7 0.04802794848160955 8 0.04534555531349841 9 0.03328010123500642 10-11 0.022701125272792652 12-13 0.01488249842280951 14-15 0.012021160928424111 16-17 0.01709538420020107 18-19 0.016301650950984563 20-21 0.022832232996993593 22-23 0.019340161045641506 24-25 0.016792419053736733 26-27 0.015116366255167948 28-29 0.017284958882491618 30-31 0.015072073105100062 32-33 0.012239083226758108 34-35 0.009712601946885923 36-37 0.007506803073505227 38-39 0.016439845579196363 40-41 0.025241780360686562 42-43 0.02557486484919706 44-45 0.024157484047024724 46-47 0.02629772905830495 48-49 0.022986373159229835 50-51 0.02289601513309135 52-53 0.01942697561977456 54-55 0.02435237390732342 56-57 0.0311061934296746 58-59 0.023482456439990154 60-61 0.02338323978383809 62-63 0.03052329557478122 64-65 0.03127096394792713 66-67 0.024049408760859085 68-69 0.024793533681999564 70-71 0.03548944356039254 72-73 0.040115420153482496 74-75 0.04133436764335071 76-77 0.04234956664290664 78-79 0.03666941307820101 80-81 0.03200623023905402 82-83 0.03494906712956434 84-85 0.03832420516473721 86-87 0.038244477494615015 88-89 0.0374613746014148 90-91 0.04026070168570516 92-93 0.03383819492586177 94-95 0.03372303273568527 96-97 0.03365039196957394 98-99 0.3103939935937223 100 0.14732256057779244 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8221068E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.133787874532956 #Duplication Level Percentage of deduplicated Percentage of total 1 78.5500660320565 37.809122159175644 2 10.488500972954169 10.09702561908017 3 3.8617706432868637 5.576449468922058 4 1.9826560723949966 3.8173098726726167 5 1.207195022500482 2.9053434568115124 6 0.8013950727855459 2.3144508262293217 7 0.5449227643603521 1.8360437723407705 8 0.4040031922440668 1.5556963164888056 9 0.2958336115272822 1.281563307307007 >10 1.6071270734146783 14.37627470428186 >50 0.18386244074271624 6.130783925775274 >100 0.07130249096620037 5.0448307853787435 >500 7.482289938593515E-4 0.22898415580886708 >1k 5.285222018865461E-4 0.5011231884874823 >5k 5.125141602699589E-5 0.15783737206998608 >10k+ 3.660815430320946E-5 6.3671610691697165 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1503401 5.327229288416724 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 152654 0.5409221224370389 Illumina Single End PCR Primer 1 (100% over 50bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 75323 0.2669034354050669 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 43441 0.15393109856792095 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31889 0.11299714100118394 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.619228868305055 1.4173808021723346E-5 0.0 0.0 9.921665615206342E-5 2 0.6192678462771146 2.834761604344669E-5 0.0 0.0 9.921665615206342E-5 3 0.6192855635371418 4.252142406517003E-5 0.0 0.0 9.921665615206342E-5 4 0.619303280797169 4.252142406517003E-5 0.0 0.0 1.1339046417378677E-4 5 0.6193103677011799 6.378213609775505E-5 0.0 0.0 1.1339046417378677E-4 6 0.6193174546051907 6.378213609775505E-5 0.0 3.5434520054308364E-6 1.2047736818464844E-4 7 0.6193245415092016 7.086904010861672E-5 0.0 3.5434520054308364E-6 1.3110772420094093E-4 8 0.619328084961207 7.441249211404756E-5 0.0 3.5434520054308364E-6 1.3465117620637177E-4 9 0.6193564325772505 8.504284813034006E-5 0.0 3.5434520054308364E-6 1.381946282118026E-4 10-11 0.6193865519192966 9.212975214120174E-5 0.0 7.086904010861672E-6 1.39966354214518E-4 12-13 0.6194308450693645 1.063035601629251E-4 0.0 1.2402082019007926E-5 1.5059671023081054E-4 14-15 0.6195088010134839 1.169339161792176E-4 0.0 1.4173808021723346E-5 1.5414016223624135E-4 16-17 0.6195424638075355 1.3287945020365634E-4 0.0 1.4173808021723346E-5 1.7185742226339556E-4 18-19 0.6195938438616143 1.647705182525339E-4 0.0 1.4173808021723346E-5 1.8780295628783434E-4 20-21 0.6196806584357473 1.94889860298696E-4 0.0 2.1260712032585016E-5 1.9311813429598058E-4 22-23 0.620019058102266 2.143788463285656E-4 0.0 2.1260712032585016E-5 2.037484903122731E-4 24-25 0.6212326904141261 2.3741128436386604E-4 0.0 2.4804164038015855E-5 2.2500920234485813E-4 26-27 0.6257169289269988 2.444981883747277E-4 0.0 2.6575890040731273E-5 2.4272646237201228E-4 28-29 0.6383121290803027 2.6221544840188186E-4 0.0 2.834761604344669E-5 2.480416403801585E-4 30-31 0.6690852380214668 2.8170443443175145E-4 0.0 2.834761604344669E-5 2.5335681838830476E-4 32-33 0.7308582368321426 3.1536722848334443E-4 0.0 3.0119342046162106E-5 2.7461753042088983E-4 34-35 0.8358507197530582 3.4017139252136026E-4 0.0 3.366279405159294E-5 2.976499684561902E-4 36-37 0.989291050218227 3.543452005430836E-4 0.0 3.8977972059739196E-5 3.13595502480629E-4 38-39 1.1978072552038073 3.6497555655937614E-4 0.0 4.0749698062454614E-5 3.2776931050235234E-4 40-41 1.4621576334389612 3.6851900856480694E-4 0.0 4.606487607060087E-5 3.3308448851049864E-4 42-43 1.7850830450498898 3.968666246082537E-4 0.0 5.138005407874713E-5 3.437148445267911E-4 44-45 2.164083584646761 4.1104043262997703E-4 0.0 5.315178008146255E-5 3.702907345675224E-4 46-47 2.599901605424713 4.2344251464898494E-4 0.0 5.669523208689338E-5 3.7914936458109953E-4 48-49 3.0899326701597545 4.4470322668156996E-4 0.0 5.669523208689338E-5 3.9155144660010745E-4 50-51 3.6351175653593266 4.65963938714155E-4 0.0 5.669523208689338E-5 3.9863835061096906E-4 52-53 4.225277725137829 4.712791167223012E-4 0.0 5.84669580896088E-5 4.057252546218308E-4 54-55 4.859472362987821 4.8545292474402456E-4 0.0 6.023868409232422E-5 4.092687066272616E-4 56-57 5.537917629481634 5.049419107738941E-4 0.0 6.023868409232422E-5 4.181273366408387E-4 58-59 6.259476076525523 5.226591708010484E-4 0.0 6.023868409232422E-5 4.358445966679929E-4 60-61 7.017762403605703 5.403764308282025E-4 0.0 6.201041009503963E-5 4.464749526842854E-4 62-63 7.804419379167365 5.669523208689338E-4 0.0 6.378213609775505E-5 4.53561856695147E-4 64-65 8.61595138780715 5.93528210909665E-4 0.0 6.90973141059013E-5 4.5710530870057787E-4 66-67 9.453598637726962 6.183323749476808E-4 0.0 7.086904010861672E-5 4.6064876070600873E-4 68-69 10.31844365351446 6.307344569666889E-4 0.0 7.441249211404756E-5 4.695073907195858E-4 70-71 11.2026306020736 6.431365389856968E-4 0.0 7.441249211404756E-5 4.7836602073316294E-4 72-73 12.106198461376444 6.537668950019893E-4 0.0 7.441249211404756E-5 4.907681027521709E-4 74-75 13.023548577254411 6.714841550291435E-4 0.0 7.795594411947839E-5 5.049419107738942E-4 76-77 13.950453611465022 6.980600450698748E-4 0.0 7.972767012219381E-5 5.20887444798333E-4 78-79 14.8903843752476 7.104621270888826E-4 0.0 8.149939612490923E-5 5.244308968037637E-4 80-81 15.836354952973432 7.352662911268985E-4 0.0 8.149939612490923E-5 5.244308968037637E-4 82-83 16.787913554511828 7.512118251513373E-4 0.0 8.149939612490923E-5 5.386047048254871E-4 84-85 17.749932426370258 7.600704551649144E-4 0.0 8.85863001357709E-5 5.421481568309179E-4 86-87 18.717574756561305 7.901897972110764E-4 0.0 9.212975214120175E-5 5.439198828336334E-4 88 19.44421451378098 8.043636052327999E-4 0.0 9.212975214120175E-5 5.492350608417797E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 191875 0.0 84.51726 8 GTGGTAT 192910 0.0 84.11083 6 AGTGGTA 193790 0.0 83.87902 5 TGGTATC 193980 0.0 83.50264 7 GTATCAA 193790 0.0 83.480415 9 AAGCAGT 196910 0.0 83.38996 1 GCAGTGG 204095 0.0 80.427055 3 CAGTGGT 204355 0.0 79.80928 4 AGCAGTG 211355 0.0 78.06654 2 CAACGCA 190120 0.0 44.95956 12-13 ATCAACG 191875 0.0 44.570805 10-11 ACGCAGA 193595 0.0 44.15669 14-15 AGAGTAC 193520 0.0 43.65316 18-19 TCAACGC 189280 0.0 42.798424 12-13 AGTACTT 192560 0.0 42.55653 20-21 AACGCAG 191880 0.0 42.149063 14-15 GAGTACT 185425 0.0 41.822117 20-21 TATCAAC 195385 0.0 41.4013 10-11 CGCAGAG 195880 0.0 41.227116 16-17 GCAGAGT 208385 0.0 40.800583 16-17 >>END_MODULE