FastQCFastQC Report
Thu 26 May 2016
SRR1660670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28221068
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13827924.899857085493717No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTCATCTCGTATG1194500.4232653420487134TruSeq Adapter, Index 7 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690580.24470370859104268No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT443160.15703161907267293No Hit
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACCACCTCATCTCGTATG421660.14941319726099664TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342740.12144827403413648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1783050.083.550548
GTGGTAT1799800.082.940016
AGTGGTA1808000.082.6963655
AAGCAGT1824850.082.535361
TGGTATC1808450.082.4056247
GTATCAA1808400.082.109179
GCAGTGG1896750.079.302493
CAGTGGT1913100.078.396374
AGCAGTG1970750.076.611822
CAACGCA1752300.044.7725712-13
ATCAACG1773700.044.27456310-11
ACGCAGA1794250.043.8070714-15
AGAGTAC1807150.043.20664618-19
TCAACGC1749750.042.33288612-13
AGTACTT1795300.042.27277420-21
AACGCAG1768750.041.79186614-15
GAGTACT1725250.041.46051820-21
TATCAAC1818650.040.8038210-11
CGCAGAG1814750.040.75432616-17
GCAGAGT1954300.040.20607816-17
Produced by FastQC (version 0.11.5)