##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660670_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28221068
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79785297282158	34.0	31.0	34.0	31.0	34.0
2	32.97747023606619	34.0	31.0	34.0	31.0	34.0
3	33.030975936133956	34.0	33.0	34.0	31.0	34.0
4	36.409083454956416	37.0	37.0	37.0	35.0	37.0
5	36.33455803302696	37.0	37.0	37.0	35.0	37.0
6	36.32476627036227	37.0	37.0	37.0	35.0	37.0
7	36.24616995359637	37.0	37.0	37.0	35.0	37.0
8	36.281203886401464	37.0	37.0	37.0	35.0	37.0
9	38.06832289267012	39.0	38.0	39.0	35.0	39.0
10-11	37.980529723396714	39.0	38.0	39.0	35.0	39.0
12-13	38.05641565372366	39.0	38.0	39.0	35.0	39.0
14-15	39.58067752786677	41.0	40.0	41.0	37.0	41.0
16-17	39.553759659273	41.0	39.5	41.0	37.0	41.0
18-19	39.501197350149894	41.0	39.0	41.0	37.0	41.0
20-21	39.452670111563464	41.0	39.0	41.0	36.5	41.0
22-23	39.29861387598797	40.5	39.0	41.0	36.0	41.0
24-25	39.31517613011669	41.0	39.0	41.0	36.0	41.0
26-27	39.28519322869	41.0	39.0	41.0	36.0	41.0
28-29	39.22322897205733	41.0	39.0	41.0	36.0	41.0
30-31	39.13558973033905	40.0	39.0	41.0	35.5	41.0
32-33	39.04192523826525	40.0	39.0	41.0	35.0	41.0
34-35	38.945873699748006	40.0	38.5	41.0	35.0	41.0
36-37	38.770822829951015	40.0	38.0	41.0	35.0	41.0
38-39	38.418026011630744	40.0	38.0	41.0	35.0	41.0
40-41	38.30146415791209	40.0	37.5	41.0	34.5	41.0
42-43	38.13984975692628	40.0	37.0	41.0	34.0	41.0
44-45	38.03252105838092	40.0	37.0	41.0	34.0	41.0
46-47	37.89387759882085	40.0	37.0	41.0	33.5	41.0
48-49	37.738548874195686	40.0	36.0	41.0	33.0	41.0
50-51	37.771546615457645	40.0	36.5	41.0	33.0	41.0
52-53	37.6388480407616	40.0	36.0	41.0	33.0	41.0
54-55	37.087401741847614	40.0	36.0	41.0	31.5	41.0
56-57	36.72704045077245	39.5	36.0	41.0	31.0	41.0
58-59	36.5029479394614	39.0	35.0	41.0	31.0	41.0
60-61	36.297491310392644	39.0	35.0	41.0	30.0	41.0
62-63	36.061888515346055	38.5	35.0	41.0	30.0	41.0
64-65	35.78721349241637	38.0	35.0	40.0	29.5	41.0
66-67	35.506625989491255	37.0	35.0	40.0	29.0	41.0
68-69	35.205108148281276	37.0	35.0	39.5	29.0	41.0
70-71	34.844466605587	36.0	35.0	39.0	29.0	41.0
72-73	34.43320015387086	35.5	34.0	39.0	28.0	40.5
74-75	34.021740814344795	35.0	34.0	37.5	27.5	40.0
76-77	32.1598823439283	34.5	32.0	35.5	25.0	38.5
78-79	33.27270245406729	35.0	34.0	37.0	26.5	39.0
80-81	33.232744008837656	35.0	34.0	36.0	27.0	39.0
82-83	33.00217963048032	35.0	34.0	36.0	27.0	37.0
84-85	32.77016534243141	35.0	34.0	35.5	26.0	37.0
86-87	32.576494624512435	35.0	34.0	35.0	26.0	36.5
88-89	32.40803888073974	35.0	34.0	35.0	25.5	36.0
90-91	32.28570924388829	35.0	34.0	35.0	25.0	36.0
92-93	32.18043856455043	35.0	34.0	35.0	25.0	36.0
94-95	32.073164913531976	35.0	34.0	35.0	25.0	35.0
96-97	31.9910097838962	35.0	33.5	35.0	24.5	35.0
98-99	31.88763857200585	35.0	33.0	35.0	24.0	35.0
100	31.82817822486378	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3823916100482805
1101	2	0.3402548525451863
1101	3	0.32188366372751887
1101	4	0.21682652101218736
1101	5	0.23407077068422666
1101	6	0.26021884711864374
1101	7	0.2640914769506537
1101	8	0.2614386154565054
1101	9	0.31706072917034334
1101	10-11	0.3314812435553307
1101	12-13	0.3326166260998491
1101	14-15	0.4053596068308636
1101	16-17	0.4169118606616564
1101	18-19	0.43609205784659366
1101	20-21	0.4377850662840004
1101	22-23	0.4483340521328216
1101	24-25	0.4519423973557153
1101	26-27	0.47716342571195725
1101	28-29	0.4879710115931388
1101	30-31	0.5013752346393332
1101	32-33	0.47421750198957824
1101	34-35	0.5039960235615482
1101	36-37	0.5394970607325931
1101	38-39	0.5242688323076692
1101	40-41	0.5242481107925769
1101	42-43	0.555086860468343
1101	44-45	0.5482313383522026
1101	46-47	0.5173794849487763
1101	48-49	0.5392196616321598
1101	50-51	0.49741031384979095
1101	52-53	0.5706707384361067
1101	54-55	0.48944891407737146
1101	56-57	0.45548253411354267
1101	58-59	0.46155432942209984
1101	60-61	0.4077971322852605
1101	62-63	0.3415857466268051
1101	64-65	0.36075160203033363
1101	66-67	0.3582963114616149
1101	68-69	0.3766118411929895
1101	70-71	0.3557438789286138
1101	72-73	0.3628436698829276
1101	74-75	0.3422671889965656
1101	76-77	0.39691441231597224
1101	78-79	0.4506140617788148
1101	80-81	0.40008611421159657
1101	82-83	0.38048341109610107
1101	84-85	0.34451878175207185
1101	86-87	0.34767816546007424
1101	88-89	0.36901314612422453
1101	90-91	0.34785868419532306
1101	92-93	0.3341225693642258
1101	94-95	0.35445445994641744
1101	96-97	0.3513635018478922
1101	98-99	0.3263605815164361
1101	100	0.34986647412500105
1102	1	0.33142663943743855
1102	2	0.26075943635796506
1102	3	0.27189306787027334
1102	4	0.19086229693193246
1102	5	0.20590805818392255
1102	6	0.20800160319543437
1102	7	0.2229228167363715
1102	8	0.2206366723886788
1102	9	0.27557225807449726
1102	10-11	0.2934864091393692
1102	12-13	0.2724117421519878
1102	14-15	0.3443163259191877
1102	16-17	0.36043494822144595
1102	18-19	0.39408502784355903
1102	20-21	0.3938238747536644
1102	22-23	0.39333428568166795
1102	24-25	0.399823057901024
1102	26-27	0.39638788489901344
1102	28-29	0.40130724722417455
1102	30-31	0.4042049707195261
1102	32-33	0.4140316458181772
1102	34-35	0.4180805352556547
1102	36-37	0.42569225055878235
1102	38-39	0.4276187672061198
1102	40-41	0.44130246383203087
1102	42-43	0.40651683000957917
1102	44-45	0.408072890540474
1102	46-47	0.42261651120612953
1102	48-49	0.43049322232824494
1102	50-51	0.4350786324577385
1102	52-53	0.42500288025749455
1102	54-55	0.3704902500900431
1102	56-57	0.405868219982942
1102	58-59	0.39158045438673383
1102	60-61	0.3264044811208606
1102	62-63	0.32252883848255465
1102	64-65	0.31692522603283635
1102	66-67	0.28317624807345965
1102	68-69	0.29597254887534774
1102	70-71	0.31210922306500066
1102	72-73	0.2709624170882634
1102	74-75	0.3264723827820646
1102	76-77	0.32026972599735615
1102	78-79	0.36805211115882486
1102	80-81	0.3400110633516178
1102	82-83	0.31908913799797034
1102	84-85	0.3653236208464321
1102	86-87	0.3643236454998302
1102	88-89	0.3907329458577422
1102	90-91	0.3718738640233852
1102	92-93	0.3336057214413941
1102	94-95	0.3871173612559957
1102	96-97	0.39211616919357795
1102	98-99	0.3633179228930068
1102	100	0.38763762947705516
1103	1	0.26024521823538294
1103	2	0.24182741024914378
1103	3	0.2338037104010411
1103	4	0.16113914616098413
1103	5	0.17235782088297213
1103	6	0.17680132340213106
1103	7	0.19357430022077438
1103	8	0.15281446639366436
1103	9	0.23476765718007186
1103	10-11	0.2483435245886909
1103	12-13	0.24864654425315535
1103	14-15	0.314715881628743
1103	16-17	0.28457283375994535
1103	18-19	0.30530320122609567
1103	20-21	0.31824084903620786
1103	22-23	0.3166662465507031
1103	24-25	0.32187685410996636
1103	26-27	0.2766595459533363
1103	28-29	0.3352777215226723
1103	30-31	0.347303578479746
1103	32-33	0.30712119355739986
1103	34-35	0.3478981450991512
1103	36-37	0.32588160117904863
1103	38-39	0.3757158381996675
1103	40-41	0.33395010307982886
1103	42-43	0.3350137856993243
1103	44-45	0.3490797361166855
1103	46-47	0.30499795901734217
1103	48-49	0.31861885615976604
1103	50-51	0.3715563849890202
1103	52-53	0.34508634624997114
1103	54-55	0.2149622921158567
1103	56-57	0.24235069441768786
1103	58-59	0.2597418956361608
1103	60-61	0.17349201659981617
1103	62-63	0.14523162337644635
1103	64-65	0.16183902570884356
1103	66-67	0.16512510136216463
1103	68-69	0.1850044607133441
1103	70-71	0.12173853703124138
1103	72-73	0.1425085924447913
1103	74-75	0.14670706997657845
1103	76-77	0.05543570586860369
1103	78-79	0.225976752099875
1103	80-81	0.22608470935058023
1103	82-83	0.2210258478035172
1103	84-85	0.2211840414945172
1103	86-87	0.2727696309816139
1103	88-89	0.29953582170126225
1103	90-91	0.23673665377626918
1103	92-93	0.21720680942294024
1103	94-95	0.22352394536515163
1103	96-97	0.23361603361785654
1103	98-99	0.24395286596771726
1103	100	0.22314925344581837
1104	1	0.23246590763066877
1104	2	0.20775181777108287
1104	3	0.19713005320429744
1104	4	0.14629544247362958
1104	5	0.15523484784085184
1104	6	0.16445577675557388
1104	7	0.17747878515162085
1104	8	0.15678808533944277
1104	9	0.19780527833412975
1104	10-11	0.20364686297244816
1104	12-13	0.2059827153841738
1104	14-15	0.2217418035762364
1104	16-17	0.264296327494165
1104	18-19	0.25411516849995763
1104	20-21	0.29127526496172607
1104	22-23	0.29652410334784207
1104	24-25	0.27832500439607344
1104	26-27	0.25460879192977615
1104	28-29	0.26989571897359355
1104	30-31	0.2822003470269294
1104	32-33	0.2919645536350757
1104	34-35	0.29759313258862363
1104	36-37	0.3134788760081335
1104	38-39	0.2811778552937909
1104	40-41	0.250322411024257
1104	42-43	0.26597592447308926
1104	44-45	0.31781032021172706
1104	46-47	0.31107237031179125
1104	48-49	0.3250585074742105
1104	50-51	0.29997257049723913
1104	52-53	0.31439771672145866
1104	54-55	0.20696520704213128
1104	56-57	0.22198958484757014
1104	58-59	0.19324849098364183
1104	60-61	0.12947204853446692
1104	62-63	0.1498615486920727
1104	64-65	0.17808807058864318
1104	66-67	0.14100184176649577
1104	68-69	0.12753379993004899
1104	70-71	0.16442170749511575
1104	72-73	0.18463604087529717
1104	74-75	0.17824226583241654
1104	76-77	0.17738927703270946
1104	78-79	0.2423371291722205
1104	80-81	0.22996458048456958
1104	82-83	0.21048910920807629
1104	84-85	0.23134843602909427
1104	86-87	0.24083417846130573
1104	88-89	0.29563327288818897
1104	90-91	0.2674745447488931
1104	92-93	0.24633610635180503
1104	94-95	0.27435737577363994
1104	96-97	0.2786034451105692
1104	98-99	0.2619259757165473
1104	100	0.26371547043631693
1105	1	0.1891367175214782
1105	2	0.16235738273132938
1105	3	0.13890726102982143
1105	4	0.1330669616432516
1105	5	0.08942342026429628
1105	6	0.100449878544417
1105	7	0.11109029111018742
1105	8	0.12169690231804253
1105	9	0.14143641850927224
1105	10-11	0.16429745256434103
1105	12-13	0.14980942220761762
1105	14-15	0.18255271770676984
1105	16-17	0.1846757615564414
1105	18-19	0.22539228881554152
1105	20-21	0.2224082668880598
1105	22-23	0.2269925861668014
1105	24-25	0.22778955736642814
1105	26-27	0.18152315733443203
1105	28-29	0.1973978157995333
1105	30-31	0.21357813357947464
1105	32-33	0.227600440231555
1105	34-35	0.21932015870621768
1105	36-37	0.20302948034122892
1105	38-39	0.19467017250114793
1105	40-41	0.20991576279478608
1105	42-43	0.23942369132418406
1105	44-45	0.27895766118786725
1105	46-47	0.24276567402824867
1105	48-49	0.2175754086604016
1105	50-51	0.2113564162731265
1105	52-53	0.23130797099236133
1105	54-55	0.13542795220369186
1105	56-57	0.1166741400143323
1105	58-59	0.10794733630059028
1105	60-61	0.10335171746773142
1105	62-63	0.028936593514181652
1105	64-65	0.08432950997139699
1105	66-67	0.09423039212602191
1105	68-69	0.06530113695958306
1105	70-71	0.08633882452220831
1105	72-73	0.08166662539793634
1105	74-75	0.11246075743802919
1105	76-77	0.11193628749834517
1105	78-79	0.18106708835021124
1105	80-81	0.19550018490986787
1105	82-83	0.18468565787282643
1105	84-85	0.19738836236589208
1105	86-87	0.2172621352609383
1105	88-89	0.2563051350886596
1105	90-91	0.23554845336793306
1105	92-93	0.19421308346281307
1105	94-95	0.20008032228754047
1105	96-97	0.19782297761592815
1105	98-99	0.22771632933925545
1105	100	0.25796980317195306
1106	1	0.19545997110765967
1106	2	0.18226389355500316
1106	3	0.15747751028764867
1106	4	0.11815386796227756
1106	5	0.1166227928352086
1106	6	0.12337378203055493
1106	7	0.14334333515991915
1106	8	0.14470110143820847
1106	9	0.19613572960503944
1106	10-11	0.17692479976088293
1106	12-13	0.16400739408178566
1106	14-15	0.22174424171475948
1106	16-17	0.21105968876729975
1106	18-19	0.2263887028814935
1106	20-21	0.21403105787346277
1106	22-23	0.23524013547595501
1106	24-25	0.24316036659792672
1106	26-27	0.23201988819470643
1106	28-29	0.23269572735180333
1106	30-31	0.24663083946374087
1106	32-33	0.2596410990347735
1106	34-35	0.23703216413133532
1106	36-37	0.25410232995076854
1106	38-39	0.27097915522492855
1106	40-41	0.2537541085619779
1106	42-43	0.2661143520713196
1106	44-45	0.2631572969045024
1106	46-47	0.27052290509043075
1106	48-49	0.23613984319919723
1106	50-51	0.2590298008595582
1106	52-53	0.294851407856612
1106	54-55	0.17386983567737957
1106	56-57	0.17439602300317603
1106	58-59	0.1740092505592088
1106	60-61	0.16698828850440606
1106	62-63	0.12609612015852179
1106	64-65	0.18363607939112825
1106	66-67	0.14004351982637075
1106	68-69	0.15891212804886123
1106	70-71	0.12494806387092439
1106	72-73	0.1798455975463895
1106	74-75	0.18793824035750362
1106	76-77	0.11089167665286226
1106	78-79	0.24067183488946853
1106	80-81	0.24749770361523105
1106	82-83	0.2441129927689829
1106	84-85	0.28382327942822627
1106	86-87	0.31988420830496267
1106	88-89	0.3048019788785652
1106	90-91	0.31262301902363987
1106	92-93	0.26514005122059103
1106	94-95	0.2619317277564761
1106	96-97	0.257998892784137
1106	98-99	0.29313907603578926
1106	100	0.30453380893667514
1107	1	0.16213728021406126
1107	2	0.1426959389106912
1107	3	0.12772040059441991
1107	4	0.09490902602575346
1107	5	0.09327136349732967
1107	6	0.08437339987455061
1107	7	0.10649221592625935
1107	8	0.10928074183399161
1107	9	0.1442215645485163
1107	10-11	0.13682794934645415
1107	12-13	0.11881491187430981
1107	14-15	0.17229922662045993
1107	16-17	0.158007989634946
1107	18-19	0.19255792505666847
1107	20-21	0.17638976753617897
1107	22-23	0.18036005312109893
1107	24-25	0.167824040230947
1107	26-27	0.1711471202405619
1107	28-29	0.17477619509909204
1107	30-31	0.1759436373136012
1107	32-33	0.15699203517992544
1107	34-35	0.18270966826055002
1107	36-37	0.17900794916755558
1107	38-39	0.15038173860861548
1107	40-41	0.19284895647564326
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2314	2	-0.4612387030018468
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2314	92-93	-0.3256434285980916
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2314	96-97	-0.31812046742703615
2314	98-99	-0.33484396132094574
2314	100	-0.3169430505985673
2315	1	-1.0009913377711221
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2315	3	-0.4655763556178627
2315	4	-0.28529936590945226
2315	5	-0.29265721609883855
2315	6	-0.27757716285567113
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2315	9	-0.3582655455673276
2315	10-11	-0.3580540682710307
2315	12-13	-0.3363714301734291
2315	14-15	-0.41119415777097856
2315	16-17	-0.44630326056455516
2315	18-19	-0.4584334342835703
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2315	24-25	-0.43365088095975324
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2315	28-29	-0.49897792808926766
2315	30-31	-0.48302551476590594
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2315	96-97	-0.34612819047880805
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2315	100	-0.3975172754930618
2316	1	-1.552945881899987
2316	2	-0.8243394307361598
2316	3	-0.5740668733214704
2316	4	-0.3334048112818948
2316	5	-0.32299892949549047
2316	6	-0.2954835423310129
2316	7	-0.32046387459475767
2316	8	-0.313426124654967
2316	9	-0.37889440586261713
2316	10-11	-0.3951808423258498
2316	12-13	-0.3790177194717188
2316	14-15	-0.4831970404694701
2316	16-17	-0.509756429954443
2316	18-19	-0.46822005613694273
2316	20-21	-0.49421286948706467
2316	22-23	-0.5208559633624006
2316	24-25	-0.5366340675838117
2316	26-27	-0.5317362699580883
2316	28-29	-0.5526342010727987
2316	30-31	-0.560876472284221
2316	32-33	-0.5317832955923762
2316	34-35	-0.52429710848876
2316	36-37	-0.5170472861374549
2316	38-39	-0.5948899645578223
2316	40-41	-0.5924810146421038
2316	42-43	-0.6295892681555486
2316	44-45	-0.5442753987881233
2316	46-47	-0.49938468905662603
2316	48-49	-0.5294732691713406
2316	50-51	-0.5175875297912
2316	52-53	-0.4663028531034712
2316	54-55	-0.4596780555876734
2316	56-57	-0.4364116659370083
2316	58-59	-0.38820698411554844
2316	60-61	-0.31614365636614394
2316	62-63	-0.309540692289211
2316	64-65	-0.3111469041729009
2316	66-67	-0.33508650788824923
2316	68-69	-0.35465290018944273
2316	70-71	-0.36391993765287367
2316	72-73	-0.38690013452976046
2316	74-75	-0.3972200007755191
2316	76-77	-0.43904559839924673
2316	78-79	-0.5735984315407805
2316	80-81	-0.5706533346781342
2316	82-83	-0.5321775106373821
2316	84-85	-0.5089733720384899
2316	86-87	-0.5333709361373593
2316	88-89	-0.5952199274147816
2316	90-91	-0.4985789107629195
2316	92-93	-0.4267586772265375
2316	94-95	-0.43888376417109143
2316	96-97	-0.4878969217897904
2316	98-99	-1.2329456554722817
2316	100	-0.7757586934505802
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	10.0
7	252.0
8	502.0
9	209.0
10	61.0
11	59.0
12	121.0
13	403.0
14	1710.0
15	5530.0
16	11765.0
17	20201.0
18	27901.0
19	37047.0
20	46249.0
21	63312.0
22	94842.0
23	158746.0
24	283641.0
25	411865.0
26	449490.0
27	375115.0
28	324164.0
29	347317.0
30	412185.0
31	444922.0
32	528370.0
33	674601.0
34	945596.0
35	1593685.0
36	3095145.0
37	6844221.0
38	8855739.0
39	2163573.0
40	2519.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.314352719229053	31.417028574659582	28.13867087187136	24.12994783424001
2	22.37431806513653	33.652077281234924	24.91699548734881	19.05660916627973
3	25.9801400854142	25.073190001172176	26.546284499225898	22.400385414187728
4	19.76155544503135	27.47326926110663	23.833098733187562	28.932076560674457
5	22.666009663418833	31.212436751153426	25.541248120021535	20.580305465406198
6	29.72202894660117	27.349400809352787	24.123516516100665	18.805053727945378
7	22.632155522958946	24.964264286525232	30.882137415919196	21.521442774596625
8	27.668818203478335	27.160293862726952	24.11249283691177	21.05839509688294
9	26.29511753417695	25.289829569880208	26.644151100163892	21.770901795778954
10-11	21.720988730830456	29.403702935693293	27.675083026623938	21.200225306852314
12-13	21.4510255246187	26.35038121165365	27.73332851896321	24.465264744764443
14-15	21.10544682433705	32.08908677729701	25.14538960750883	21.6600767908571
16-17	24.02973728705094	26.26490783410465	25.238086311970903	24.467268566873514
18-19	21.424033645035795	28.86553014709781	25.687380810098276	24.02305539776812
20-21	23.90345781430335	29.053162701409864	25.624437210413014	21.41894227387377
22-23	24.080408734828744	26.621254481107837	28.048944114508007	21.24939266955542
24-25	21.223427489614327	29.0222632226453	25.264930921456752	24.48937836628362
26-27	21.203284305516796	26.06991079965243	31.23807756348431	21.488727331346468
28-29	21.26568088478654	26.445147319162114	30.839298880667748	21.449872915383594
30-31	21.44875174187934	26.037966599788092	31.24422215924609	21.269059499086477
32-33	21.25795859097261	26.599045313711713	30.648640125482306	21.49435596983337
34-35	21.27100561529426	26.426303098883512	30.519897549885005	21.782793735937226
36-37	21.17058186314678	26.71067059927229	30.659134931306536	21.459612606274398
38-39	21.234229078436154	26.424051420765743	31.008249394902794	21.333470105895312
40-41	21.230425107860405	26.83321404318024	30.616118593225234	21.32024225573412
42-43	21.232470368297022	26.53125405212921	30.890811225436178	21.34546435413759
44-45	21.299557211163737	26.6476115558479	30.810635331075776	21.242195901912588
46-47	21.660572904384072	26.776613108613358	30.67125516972124	20.891558817281332
48-49	21.309522758968544	27.204159245000003	30.57778939750969	20.90852859852176
50-51	21.717761233721504	27.01302329203006	30.082636620114055	21.186578854134382
52-53	21.829289720074993	27.151604906154862	29.774620509486677	21.244484864283468
54-55	21.69354878331336	27.47183591386506	29.413668278779546	21.420947024042032
56-57	22.040368710494754	28.052272874880778	28.719987522693952	21.187370891930517
58-59	22.139918839857557	28.395898318574798	27.92156587469497	21.54261696687267
60-61	22.38724611463384	28.509750530523288	27.511381156086113	21.591622198756767
62-63	22.010972325522253	28.63343544519388	28.017197992379383	21.33839423690448
64-65	22.313165698138032	28.99158337078106	27.29589778309173	21.399353147989185
66-67	21.869044515963154	29.211426443599226	27.42353201939871	21.495997021038914
68-69	21.78175492688344	29.157904794843432	27.515007173950167	21.545333104322964
70-71	21.684122127732007	29.090999046053252	27.53732834710884	21.687550479105898
72-73	21.582270786131332	29.006111920850923	27.499409827279663	21.91220746573808
74-75	21.58242443708536	29.042736989001984	27.307065977029314	22.06777259688334
76-77	21.56616316721869	29.019284425654153	27.115000506243593	22.299551900883564
78-79	21.540339867909868	29.127543844292823	26.796713232862995	22.535403054934314
80-81	21.529368571708368	29.250101973130686	26.479464416086024	22.741065039074922
82-83	21.473590058699507	29.26952049481138	26.200088228825724	23.056801217663388
84-85	21.404323269078642	29.17480203330895	26.073304974655926	23.347569722956482
86-87	21.365993297025824	29.078059873056596	26.010090782242916	23.545856047674665
88-89	21.305630682742017	28.96124734109291	25.958785635671315	23.774336340493754
90-91	21.24957222601137	28.813946951330195	25.951375108997652	23.985105713660783
92-93	21.211385535716516	28.719984344299267	25.909561042393975	24.159069077590246
94-95	21.164362035953932	28.57793169582638	25.89191099052423	24.365795277695458
96-97	21.049296966833182	28.430795741538073	25.939388044312427	24.580519247316314
98-99	21.000108980540464	28.398316132828267	25.903069259481036	24.698505627150233
100	20.90321022413994	28.41841260265911	25.81459334789103	24.863783825309923
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	84010.0
1	55407.0
2	20530.5
3	12920.0
4	11383.0
5	10892.5
6	10815.0
7	11043.0
8	10965.5
9	11113.0
10	11985.5
11	17788.5
12	160746.5
13	186929.5
14	73576.0
15	77039.0
16	86143.5
17	93425.0
18	99692.5
19	106024.0
20	114248.5
21	126461.0
22	143488.0
23	162394.0
24	180767.0
25	201584.0
26	224652.5
27	251534.0
28	285705.0
29	320906.0
30	353237.0
31	388189.5
32	426790.0
33	462346.5
34	495135.0
35	536172.0
36	583957.0
37	621501.5
38	660478.0
39	702226.0
40	734241.5
41	771778.5
42	817684.5
43	846685.0
44	866586.0
45	898467.5
46	928385.5
47	950770.0
48	974058.5
49	999377.5
50	1022163.5
51	1044874.5
52	1055618.5
53	1040462.0
54	1003975.5
55	951450.5
56	887263.0
57	808001.0
58	710110.5
59	599635.0
60	485799.5
61	382483.0
62	294137.5
63	219264.5
64	161088.5
65	116378.5
66	81674.5
67	57848.5
68	41531.0
69	30145.5
70	22632.5
71	17594.0
72	13616.5
73	9834.0
74	7536.0
75	6299.5
76	4414.0
77	2204.5
78	1030.5
79	642.0
80	393.0
81	249.0
82	161.0
83	100.0
84	72.5
85	54.0
86	33.0
87	22.0
88	15.5
89	11.0
90	6.0
91	3.0
92	1.5
93	1.5
94	2.5
95	1.5
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10620080005476759
2	0.07368254100092882
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	2.9942169445890566E-4
20-21	0.0021136691212394936
22-23	8.096787832409461E-4
24-25	7.831028932002148E-4
26-27	0.006915046588598277
28-29	0.006652831140196394
30-31	0.008025918792300845
32-33	0.008041864326325284
34-35	0.007320771843220108
36-37	0.011110493763028386
38-39	0.013846038711220993
40-41	0.017194600856353135
42-43	0.02386337753057397
44-45	0.012194790076690224
46-47	0.01888659918894636
48-49	0.016643594069508636
50-51	0.019239172663486726
52-53	0.021616828959130818
54-55	0.1100046957825976
56-57	0.10306130157795587
58-59	0.1002389420556302
60-61	0.14232452152413225
62-63	0.0938943912399063
64-65	0.07315633837812234
66-67	0.08262621386263623
68-69	0.0760832297346082
70-71	0.05347423421395675
72-73	0.03184854662481236
74-75	0.020394338017257176
76-77	0.012143410022611477
78-79	0.008709805029348995
80-81	0.005756337782822393
82-83	4.6950739071958584E-4
84-85	0.0023528521316060753
86-87	0.0016335313745036155
88-89	0.0020959518612123397
90-91	0.0030137059306189263
92-93	0.004661411113144265
94-95	0.002009137287079284
96-97	0.0011179591077134289
98-99	0.0015360864443542677
100	0.0017150307706285247
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.8221068E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.59595544507271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.93396035628223	37.09341304775672
2	10.772327533747614	10.254384426719628
3	4.006790339398	5.7212104341520496
4	1.994712099157351	3.797609127889629
5	1.2103652963296787	2.8804246358184806
6	0.8154547903180287	2.32874099204688
7	0.5613006437305273	1.870094830120217
8	0.4429557209584651	1.6866320607103331
9	0.3110547758050716	1.3324454325175743
>10	1.6852684414505394	14.753765407984618
>50	0.18908401897891206	6.251534135068744
>100	0.07535369303778208	5.26699550099144
>500	7.705893713705856E-4	0.23661794822440071
>1k	5.128481348154293E-4	0.4297585744520134
>5k	4.442664984956034E-5	0.13320474469219945
>10k+	4.4426649825832715E-5	5.963168700854988
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1382792	4.899857085493717	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTCATCTCGTATG	119450	0.4232653420487134	TruSeq Adapter, Index 7 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	69058	0.24470370859104268	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	44316	0.15703161907267293	No Hit
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACCACCTCATCTCGTATG	42166	0.14941319726099664	TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34274	0.12144827403413648	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.6242605701527667	2.1260712032585016E-5	0.0	3.5434520054308364E-6	9.212975214120175E-5
2	0.6242889177688101	3.543452005430836E-5	0.0	3.5434520054308364E-6	9.212975214120175E-5
3	0.6242924612208156	3.8977972059739196E-5	0.0	3.5434520054308364E-6	9.921665615206342E-5
4	0.6243101784808428	7.086904010861672E-5	0.0	3.5434520054308364E-6	1.0984701216835593E-4
5	0.6243137219328482	1.063035601629251E-4	0.0	3.5434520054308364E-6	1.169339161792176E-4
6	0.6243243522888645	1.1339046417378677E-4	0.0	7.086904010861673E-6	1.2047736818464844E-4
7	0.6243314391928754	1.275642721955101E-4	0.0	1.0630356016292508E-5	1.2402082019007926E-4
8	0.6243385260968862	1.381946282118026E-4	0.0	1.4173808021723346E-5	1.3110772420094093E-4
9	0.6243491564529025	1.4173808021723344E-4	0.0	1.771726002715418E-5	1.381946282118026E-4
10-11	0.6243739606169405	1.5768361424167222E-4	0.0	1.771726002715418E-5	1.39966354214518E-4
12-13	0.6244448296570491	1.8071605227697266E-4	0.0	1.9488986029869598E-5	1.4173808021723344E-4
14-15	0.6245405028611957	2.0552021631498852E-4	0.0	2.4804164038015855E-5	1.4350980621994887E-4
16-17	0.6245865677372664	2.1969402433671186E-4	0.0	2.4804164038015855E-5	1.5236843623352595E-4
18-19	0.6246485781473614	2.444981883747277E-4	0.0	2.834761604344669E-5	1.7008569626068013E-4
20-21	0.6247247623654781	2.728458044181744E-4	0.0	3.1891068048877524E-5	1.7717260027154183E-4
22-23	0.6250667054840022	2.887913384426132E-4	0.0	3.1891068048877524E-5	1.8071605227697266E-4
24-25	0.6262962833298867	3.082803244724827E-4	0.0	3.366279405159294E-5	1.8780295628783434E-4
26-27	0.6307946956507812	3.295410365050678E-4	0.0	3.8977972059739196E-5	1.9666158630141144E-4
28-29	0.6433721785440579	3.4548657052950655E-4	0.0	3.8977972059739196E-5	2.0906366832041935E-4
30-31	0.6742834821134338	3.5611692654579903E-4	0.0	3.8977972059739196E-5	2.2500920234485813E-4
32-33	0.7361787300182969	3.614321045539453E-4	0.0	4.252142406517003E-5	2.4272646237201228E-4
34-35	0.8412863751293891	3.720624605702378E-4	0.0	4.252142406517003E-5	2.569002703937356E-4
36-37	0.9947355642245714	3.9863835061096906E-4	0.0	4.252142406517003E-5	2.675306264100281E-4
38-39	1.2034289418104231	4.1104043262997703E-4	0.0	4.252142406517003E-5	2.9410651645075936E-4
40-41	1.467476354899113	4.1989906264355413E-4	0.0	4.606487607060087E-5	3.011934204616211E-4
42-43	1.7901714421296884	4.5356185669514706E-4	0.0	5.315178008146255E-5	3.1005205047519814E-4
44-45	2.168380020203346	4.819094727385937E-4	0.0	6.023868409232422E-5	3.242258584969215E-4
46-47	2.6032678848298723	5.067136367766095E-4	0.0	6.378213609775505E-5	3.295410365050678E-4
48-49	3.091727428600505	5.279743488091947E-4	0.0	6.378213609775505E-5	3.47258296532222E-4
50-51	3.6344478529303004	5.492350608417797E-4	0.0	6.732558810318588E-5	3.5434520054308365E-4
52-53	4.222333116521317	5.616371428607876E-4	0.0	7.086904010861672E-5	3.720624605702378E-4
54-55	4.853634525808875	5.740392248797955E-4	0.0	7.086904010861672E-5	3.7914936458109953E-4
56-57	5.529434605380633	5.952999369123804E-4	0.0	7.795594411947839E-5	3.844645425892457E-4
58-59	6.248509092568716	6.218758269531117E-4	0.0	7.795594411947839E-5	4.0218180261639993E-4
60-61	7.004295514259063	6.307344569666889E-4	0.0	8.149939612490923E-5	4.11040432629977E-4
62-63	7.789111666503905	6.466799909911275E-4	0.0	8.149939612490923E-5	4.3230114466256205E-4
64-65	8.599339684805692	6.821145110454359E-4	0.0	8.149939612490923E-5	4.5356185669514706E-4
66-67	9.435440217925134	6.927448670617285E-4	0.0	8.149939612490923E-5	4.6064876070600873E-4
68-69	10.29895466748459	6.962883190671593E-4	0.0	8.149939612490923E-5	4.765942947304475E-4
70-71	11.182039602470041	7.140055790943135E-4	0.0	8.149939612490923E-5	4.8722465074674E-4
72-73	12.08501570528798	7.228642091078907E-4	0.0	8.504284813034006E-5	4.996267327657479E-4
74-75	13.002776861598576	7.370380171296139E-4	0.0	8.504284813034006E-5	5.031701847711788E-4
76-77	13.930156718377916	7.565270031594836E-4	0.0	8.858630013577091E-5	5.120288147847558E-4
78-79	14.871309973102363	7.760159891893532E-4	0.0	9.567320414663259E-5	5.20887444798333E-4
80-81	15.818588084618202	7.972767012219381E-4	0.0	9.7444930149348E-5	5.279743488091946E-4
82-83	16.771608360108836	8.114505092436616E-4	0.0	9.921665615206342E-5	5.350612528200563E-4
84-85	17.735466283558083	8.256243172653848E-4	0.0	9.921665615206342E-5	5.439198828336334E-4
86-87	18.70501144747605	8.486567553006852E-4	0.0	1.0453183416020968E-4	5.580936908553567E-4
88	19.435508252203636	8.575153853142624E-4	0.0	1.0984701216835593E-4	5.704957728743646E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	178305	0.0	83.55054	8
GTGGTAT	179980	0.0	82.94001	6
AGTGGTA	180800	0.0	82.696365	5
AAGCAGT	182485	0.0	82.53536	1
TGGTATC	180845	0.0	82.405624	7
GTATCAA	180840	0.0	82.10917	9
GCAGTGG	189675	0.0	79.30249	3
CAGTGGT	191310	0.0	78.39637	4
AGCAGTG	197075	0.0	76.61182	2
CAACGCA	175230	0.0	44.77257	12-13
ATCAACG	177370	0.0	44.274563	10-11
ACGCAGA	179425	0.0	43.80707	14-15
AGAGTAC	180715	0.0	43.206646	18-19
TCAACGC	174975	0.0	42.332886	12-13
AGTACTT	179530	0.0	42.272774	20-21
AACGCAG	176875	0.0	41.791866	14-15
GAGTACT	172525	0.0	41.460518	20-21
TATCAAC	181865	0.0	40.80382	10-11
CGCAGAG	181475	0.0	40.754326	16-17
GCAGAGT	195430	0.0	40.206078	16-17
>>END_MODULE