FastQCFastQC Report
Thu 26 May 2016
SRR1660669_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660669_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57673066
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5446980.9444581982168245No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2566280.44497027433915165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA918950.071.061538
GTGGTAT965150.067.834496
GTATCAA972950.066.903459
TGGTATC982300.066.461357
AGTGGTA989150.066.292385
GCAGTGG1165700.056.8324173
AAGCAGT1193400.055.685091
CAGTGGT1192100.055.3415224
AGCAGTG1326600.050.2545472
CAACGCA840450.041.04048512-13
TCAACGC828550.039.28820812-13
ATCAACG881150.039.28646510-11
AACGCAG869150.037.5207814-15
ACGCAGA929350.037.3897414-15
AGAGTAC952150.035.95003518-19
GAGTACT880150.034.36833220-21
CGCAGAG967500.033.67545716-17
TATCAAC998250.032.767210-11
AGTACTT1072750.030.18235820-21
GTACTTT1021850.029.4558422-23
Produced by FastQC (version 0.11.5)