##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660669_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57673066 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81517453918611 34.0 31.0 34.0 30.0 34.0 2 31.898633098507368 34.0 31.0 34.0 30.0 34.0 3 31.864136076968755 34.0 31.0 34.0 29.0 34.0 4 35.29182712429403 37.0 35.0 37.0 32.0 37.0 5 35.22880928508292 37.0 35.0 37.0 32.0 37.0 6 35.21896122186394 37.0 35.0 37.0 32.0 37.0 7 35.25030495170831 37.0 35.0 37.0 32.0 37.0 8 35.19872645577747 37.0 35.0 37.0 32.0 37.0 9 36.76120886307657 39.0 37.0 39.0 32.0 39.0 10-11 36.84534607541066 39.0 37.0 39.0 33.0 39.0 12-13 36.85522238405012 39.0 37.0 39.0 33.0 39.0 14-15 38.23028799960106 40.0 38.0 41.0 33.0 41.0 16-17 38.20133129388336 40.0 38.0 41.0 33.0 41.0 18-19 38.206675252881475 40.0 38.0 41.0 33.0 41.0 20-21 38.038792960998464 40.0 38.0 41.0 33.0 41.0 22-23 38.04885894223137 40.0 38.0 41.0 33.0 41.0 24-25 38.0652131447286 40.0 38.0 41.0 33.0 41.0 26-27 38.03662833704732 40.0 38.0 41.0 32.0 41.0 28-29 37.986835076532955 40.0 38.0 41.0 32.0 41.0 30-31 37.90970406359183 40.0 38.0 41.0 32.0 41.0 32-33 37.83768731837492 40.0 38.0 41.0 32.0 41.0 34-35 37.75395307577371 40.0 38.0 41.0 32.0 41.0 36-37 37.66752714343295 40.0 38.0 41.0 31.0 41.0 38-39 37.52622686125271 40.0 37.0 41.0 31.0 41.0 40-41 37.431488079721646 40.0 37.0 41.0 31.0 41.0 42-43 37.29449080269116 40.0 37.0 41.0 30.5 41.0 44-45 37.167210305760406 40.0 36.5 41.0 30.0 41.0 46-47 37.093806465915996 40.0 36.0 41.0 30.0 41.0 48-49 37.084002600104526 40.0 36.0 41.0 30.0 41.0 50-51 36.66977306183098 39.0 35.5 40.5 30.0 41.0 52-53 36.67031066113253 39.0 35.5 40.0 30.0 41.0 54-55 36.767005173610855 39.0 35.5 41.0 30.0 41.0 56-57 36.593704745990095 39.0 35.0 41.0 29.0 41.0 58-59 36.39762709164795 39.0 35.0 41.0 29.0 41.0 60-61 36.16603601410752 39.0 35.0 41.0 28.5 41.0 62-63 35.88907669656405 38.0 35.0 40.0 28.0 41.0 64-65 35.5902448120237 37.5 34.5 40.0 28.0 41.0 66-67 35.368427898388475 37.0 34.0 40.0 28.0 41.0 68-69 35.07667325160067 37.0 34.0 39.5 28.0 41.0 70-71 34.7388771996273 36.0 34.0 39.0 27.5 41.0 72-73 34.38040079228664 35.5 34.0 39.0 27.0 41.0 74-75 34.12071174610346 35.0 34.0 38.5 27.0 40.0 76-77 33.79949488033114 35.0 33.0 37.0 26.5 39.5 78-79 33.47568573864271 35.0 33.0 37.0 26.0 39.0 80-81 33.16840882882835 35.0 33.0 36.0 26.0 39.0 82-83 32.88851358275282 35.0 33.0 36.0 26.0 37.5 84-85 32.640763003652346 35.0 33.0 35.5 26.0 37.0 86-87 32.425615719823185 35.0 33.0 35.0 25.0 37.0 88-89 32.224184934090374 35.0 32.0 35.0 25.0 36.0 90-91 32.07811258898565 35.0 32.0 35.0 25.0 36.0 92-93 32.02668795170349 35.0 32.0 35.0 25.0 36.0 94-95 31.96333473930448 35.0 32.0 35.0 25.0 36.0 96-97 31.989112690835615 35.0 32.0 35.0 25.0 35.0 98-99 31.881548468395977 35.0 32.0 35.0 25.0 35.0 100 31.822636687981873 35.0 32.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.280664627653227 1101 2 0.24896788260290492 1101 3 0.2470190202531839 1101 4 0.20736915358591546 1101 5 0.20661579474487013 1101 6 0.16776933069574085 1101 7 0.17802186020512067 1101 8 0.2085719420902592 1101 9 0.2721511798554701 1101 10-11 0.2319482643929902 1101 12-13 0.18829305912698402 1101 14-15 0.27602101596801987 1101 16-17 0.2759385828140708 1101 18-19 0.30325960660785256 1101 20-21 0.19298931700166833 1101 22-23 0.2514872373838557 1101 24-25 0.29898512271748245 1101 26-27 0.289855484654268 1101 28-29 0.27877961144546504 1101 30-31 0.32692066136335285 1101 32-33 0.3565009735592781 1101 34-35 0.35130257555263 1101 36-37 0.3446914540720343 1101 38-39 0.4217745188083697 1101 40-41 0.4665765438305485 1101 42-43 0.4696061092991286 1101 44-45 0.4441934033410462 1101 46-47 0.4850229815783962 1101 48-49 0.45847057103511446 1101 50-51 0.689799911784938 1101 52-53 0.5952843447595697 1101 54-55 0.4017546554357381 1101 56-57 0.3640067073541218 1101 58-59 0.3720718972105175 1101 60-61 0.3687244487030128 1101 62-63 0.3160101501070969 1101 64-65 0.32763997452448734 1101 66-67 0.32491133668732886 1101 68-69 0.30914910357198266 1101 70-71 0.3109630940724557 1101 72-73 0.28480102632070015 1101 74-75 0.3500397713774177 1101 76-77 0.34660400800412106 1101 78-79 0.3567266773189104 1101 80-81 0.36226529237521987 1101 82-83 0.3347173288539125 1101 84-85 0.3282847719566391 1101 86-87 0.3342091656083781 1101 88-89 0.34696954757558274 1101 90-91 0.32269903273306255 1101 92-93 0.3567292699618392 1101 94-95 0.3482423311360847 1101 96-97 0.43423963011927214 1101 98-99 0.40826314783330986 1101 100 0.4072413431895576 1102 1 0.1944978739709704 1102 2 0.1897077161015659 1102 3 0.15171158960938413 1102 4 0.13754788364691706 1102 5 0.1310910064815971 1102 6 0.10750262620365447 1102 7 0.11187427683118756 1102 8 0.1434195902672002 1102 9 0.21708662173215743 1102 10-11 0.18919364942624384 1102 12-13 0.15688083579012613 1102 14-15 0.19236699925674117 1102 16-17 0.20814348571489916 1102 18-19 0.22751537930089683 1102 20-21 0.10089086253597657 1102 22-23 0.20185514325721954 1102 24-25 0.18945342677241683 1102 26-27 0.21565288569913577 1102 28-29 0.2532236123213423 1102 30-31 0.264341620051745 1102 32-33 0.30312459143136294 1102 34-35 0.31320738596814124 1102 36-37 0.3158219844980863 1102 38-39 0.3780635496219489 1102 40-41 0.3659432000913867 1102 42-43 0.3567883669912888 1102 44-45 0.3068921163849865 1102 46-47 0.3705836770234896 1102 48-49 0.3645510795172342 1102 50-51 0.6068096188482741 1102 52-53 0.5216902967460513 1102 54-55 0.29841459766034006 1102 56-57 0.2870365237890624 1102 58-59 0.25161950842748837 1102 60-61 0.2588340734071082 1102 62-63 0.2490047887239868 1102 64-65 0.20763043348009802 1102 66-67 0.2246514659680443 1102 68-69 0.24739666319878495 1102 70-71 0.2269781300895417 1102 72-73 0.22580024857315806 1102 74-75 0.2611058862834952 1102 76-77 0.256028785923057 1102 78-79 0.2372512853074582 1102 80-81 0.24962976018856864 1102 82-83 0.24237962407462987 1102 84-85 0.2291257690738533 1102 86-87 0.2437838968928716 1102 88-89 0.2490080816542104 1102 90-91 0.23059453258003515 1102 92-93 0.2551622257505315 1102 94-95 0.24914631504586282 1102 96-97 0.32427482688716935 1102 98-99 0.36306416946862896 1102 100 0.3991537605774589 1103 1 0.09762201298639539 1103 2 0.07102166291284817 1103 3 0.052291239165541015 1103 4 0.05021216590658639 1103 5 0.03584782397655317 1103 6 0.04872174531703877 1103 7 0.04700484779327496 1103 8 0.07832462120767758 1103 9 0.1346169203987415 1103 10-11 0.08620337884800477 1103 12-13 0.05834820230979432 1103 14-15 0.13529555919105007 1103 16-17 0.1577599392029967 1103 18-19 0.15858962287772727 1103 20-21 0.03447021848624132 1103 22-23 0.1219838992831015 1103 24-25 0.13269509768936416 1103 26-27 0.14713468605827984 1103 28-29 0.1558400184421984 1103 30-31 0.17498184102746706 1103 32-33 0.19267533059818476 1103 34-35 0.2162370176188162 1103 36-37 0.2148237125680197 1103 38-39 0.2740752726333042 1103 40-41 0.2971297263519759 1103 42-43 0.2785522715653528 1103 44-45 0.2522808987737477 1103 46-47 0.3087536310461516 1103 48-49 0.3061384909619633 1103 50-51 0.49587000508140733 1103 52-53 0.3944788611875367 1103 54-55 0.24285390011676355 1103 56-57 0.1754593656015615 1103 58-59 0.18144165744745777 1103 60-61 0.1417065797595214 1103 62-63 0.13073926675104985 1103 64-65 0.14555614479089485 1103 66-67 0.18020361916666872 1103 68-69 0.17169325296784876 1103 70-71 0.17127561853555306 1103 72-73 0.19308133928847582 1103 74-75 0.21626727276123603 1103 76-77 0.1862544467160845 1103 78-79 0.16794984679656721 1103 80-81 0.20558770653032354 1103 82-83 0.20915381383536413 1103 84-85 0.20731697587920195 1103 86-87 0.1915199322068375 1103 88-89 0.18692691066063816 1103 90-91 0.22656185862580713 1103 92-93 0.19697100886106256 1103 94-95 0.2184222015627384 1103 96-97 0.29423218217825564 1103 98-99 0.29885931635470087 1103 100 0.29891786983321467 1104 1 0.06310978352300012 1104 2 0.018541376711759483 1104 3 -0.03198068104887852 1104 4 0.015423226275380841 1104 5 -0.02384105399172398 1104 6 -0.026314098048871415 1104 7 -0.028497899599088328 1104 8 0.005361593194969316 1104 9 0.08481411915140313 1104 10-11 0.02620100440091022 1104 12-13 0.015305369836433158 1104 14-15 0.03768438480809522 1104 16-17 0.0587929069524904 1104 18-19 0.06999175026876969 1104 20-21 -0.04333746571428776 1104 22-23 0.04232588949463434 1104 24-25 0.06190097390415161 1104 26-27 0.032077468379718255 1104 28-29 0.07657763376499815 1104 30-31 0.10275344078392123 1104 32-33 0.11616545199780859 1104 34-35 0.10877068223538089 1104 36-37 0.1286302326789439 1104 38-39 0.16482876332979401 1104 40-41 0.20331837456429014 1104 42-43 0.15696606961952142 1104 44-45 0.08966296130812879 1104 46-47 0.17460131495943898 1104 48-49 0.17264616996673254 1104 50-51 0.3697332705798928 1104 52-53 0.26438041916120625 1104 54-55 0.07991218411228118 1104 56-57 0.09950369505181555 1104 58-59 0.07940045354454384 1104 60-61 0.05251498100882657 1104 62-63 0.05867602973656716 1104 64-65 0.04165362313236898 1104 66-67 0.063312186989954 1104 68-69 0.0443702507167032 1104 70-71 0.024577509598636027 1104 72-73 0.02089201908317051 1104 74-75 0.08044431496451665 1104 76-77 0.04674323307316541 1104 78-79 0.09457706739178207 1104 80-81 0.1377584257026072 1104 82-83 0.10007074303879193 1104 84-85 0.08670316245758869 1104 86-87 0.07253783650910606 1104 88-89 0.08219031246927244 1104 90-91 0.11194779895063789 1104 92-93 0.12077327483920186 1104 94-95 0.11531336403743353 1104 96-97 0.2021921630930521 1104 98-99 0.19442942256597462 1104 100 0.21336015325156765 1105 1 -0.035174517875194766 1105 2 -0.07023822031418803 1105 3 -0.09575834865441024 1105 4 -0.0525989349446121 1105 5 -0.10263001754590562 1105 6 -0.0868336897915043 1105 7 -0.0961417698400453 1105 8 -0.03985944693858556 1105 9 0.002489688829179215 1105 10-11 -0.06582293027371122 1105 12-13 -0.06684107712020193 1105 14-15 -0.038522304899125004 1105 16-17 -0.02011968932210806 1105 18-19 -0.0033077891387165437 1105 20-21 -0.09333366826368916 1105 22-23 -0.008405298674958317 1105 24-25 0.010128107595264169 1105 26-27 0.031167705908003995 1105 28-29 0.0056512961063788225 1105 30-31 0.05187382206604951 1105 32-33 0.03951119261787994 1105 34-35 0.06877172676207266 1105 36-37 0.09316963274197576 1105 38-39 0.1518454894568677 1105 40-41 0.14972205381537407 1105 42-43 0.1279825173568554 1105 44-45 0.13009544650607552 1105 46-47 0.16583836188634393 1105 48-49 0.12223409139411245 1105 50-51 0.3290228367087664 1105 52-53 0.2542235607658796 1105 54-55 0.07611792495491443 1105 56-57 0.043651386642565626 1105 58-59 0.03413218834187859 1105 60-61 0.015882029851987056 1105 62-63 -0.03510094186373891 1105 64-65 -0.03491306579599751 1105 66-67 0.043375311155351426 1105 68-69 0.028521159928565964 1105 70-71 0.03252201812237132 1105 72-73 0.05590644816358292 1105 74-75 0.0886943982490962 1105 76-77 0.10701427543602193 1105 78-79 0.11549702973177034 1105 80-81 0.12388145680687046 1105 82-83 0.10270492728366065 1105 84-85 0.12548350114207807 1105 86-87 0.12743416460632062 1105 88-89 0.12954923208395286 1105 90-91 0.13536781802248754 1105 92-93 0.12232035217282089 1105 94-95 0.12059130260161766 1105 96-97 0.21179315902062257 1105 98-99 0.19521922094886435 1105 100 0.19597405548537594 1106 1 -0.05035104922625777 1106 2 -0.12315359155838124 1106 3 -0.13759563196603253 1106 4 -0.07874571422208732 1106 5 -0.11782279571032461 1106 6 -0.12365734478046164 1106 7 -0.0954368991071064 1106 8 -0.0264655068350379 1106 9 -0.026727370013340135 1106 10-11 -0.07030599875296417 1106 12-13 -0.08310138957060076 1106 14-15 -0.09723075821096927 1106 16-17 -0.06804218805712736 1106 18-19 -0.04195250127422412 1106 20-21 -0.13040791267665242 1106 22-23 -0.05715393345634823 1106 24-25 -0.03884758516714726 1106 26-27 -0.05468442678305507 1106 28-29 -0.03362741516645684 1106 30-31 0.012642588480041184 1106 32-33 0.03941713059530372 1106 34-35 0.01140647063019884 1106 36-37 0.0416374126032224 1106 38-39 0.09725628517221452 1106 40-41 0.07863419632453628 1106 42-43 0.048481293999373065 1106 44-45 0.06977086660063492 1106 46-47 0.1119397633901329 1106 48-49 0.10906431292930563 1106 50-51 0.3399090111895404 1106 52-53 0.22911351231784494 1106 54-55 0.018649256863938035 1106 56-57 0.010066117203301417 1106 58-59 -0.0019949742226330613 1106 60-61 0.012723244875616047 1106 62-63 -0.0036312381874097355 1106 64-65 -0.034601624511175544 1106 66-67 -0.012096535376137751 1106 68-69 -0.010948235016634555 1106 70-71 -0.010525091274040221 1106 72-73 0.03242624666025051 1106 74-75 0.09711383119969241 1106 76-77 0.07826257576174811 1106 78-79 0.08025869099837735 1106 80-81 0.08589055867412299 1106 82-83 0.09377427204736222 1106 84-85 0.13613060361327456 1106 86-87 0.12226926442834696 1106 88-89 0.12322435886421346 1106 90-91 0.1193526538409273 1106 92-93 0.14053291372674437 1106 94-95 0.09461268101553344 1106 96-97 0.19093763992587043 1106 98-99 0.23526882310046915 1106 100 0.23272490183512318 1107 1 -0.06932499696765149 1107 2 -0.1017927986210907 1107 3 -0.12641612678774905 1107 4 -0.09936679993649022 1107 5 -0.14742728459798116 1107 6 -0.13438026627068922 1107 7 -0.13429096201520707 1107 8 -0.0967871112586991 1107 9 -0.062225106231821314 1107 10-11 -0.11230385525806241 1107 12-13 -0.15122139482929242 1107 14-15 -0.17185765781704987 1107 16-17 -0.1438572993291487 1107 18-19 -0.12485087866315325 1107 20-21 -0.2354344155568029 1107 22-23 -0.16332357709852374 1107 24-25 -0.13464434332392727 1107 26-27 -0.12401385851381974 1107 28-29 -0.1040200704859302 1107 30-31 -0.08039997979476965 1107 32-33 -0.04423090675334862 1107 34-35 -0.049032709465087976 1107 36-37 -0.026780246311183475 1107 38-39 -0.005366569537834209 1107 40-41 -0.019714327859865932 1107 42-43 -0.04085394843722412 1107 44-45 -0.013230486844861389 1107 46-47 0.03055115795361729 1107 48-49 -0.006699885176246312 1107 50-51 0.20571676496344793 1107 52-53 0.10891619771105354 1107 54-55 -0.03433160652411971 1107 56-57 -0.06032716789301418 1107 58-59 -0.08004669610403425 1107 60-61 -0.08599906845984862 1107 62-63 -0.07062152635869268 1107 64-65 -0.09622982932562252 1107 66-67 -0.06555663254622601 1107 68-69 -0.07470801912412384 1107 70-71 -0.06447297927191187 1107 72-73 -0.056229301384441044 1107 74-75 -0.01820156265885231 1107 76-77 0.011124690901574752 1107 78-79 -0.022062744970909876 1107 80-81 0.017501273280757346 1107 82-83 -0.015453635085222572 1107 84-85 -0.006013033485309904 1107 86-87 0.028395529116799878 1107 88-89 0.03334302636526587 1107 90-91 0.06677174060953206 1107 92-93 0.06663925349762678 1107 94-95 0.04441611423668945 1107 96-97 0.08985524653334664 1107 98-99 0.11858671392842268 1107 100 0.1321106268518477 1108 1 -0.09627898026212733 1108 2 -0.11412927620744284 1108 3 -0.14674774327361106 1108 4 -0.12041051904147793 1108 5 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0.17828870441870492 2316 7 0.12206500784750318 2316 8 0.16032184794303106 2316 9 0.1613681327497929 2316 10-11 0.07347561672424519 2316 12-13 0.1914479561434419 2316 14-15 0.12863289910995945 2316 16-17 0.06713296579118122 2316 18-19 -0.11534402139562872 2316 20-21 0.02962628869560291 2316 22-23 0.031376468002683566 2316 24-25 -0.04043515516057994 2316 26-27 -0.04317914854262739 2316 28-29 -0.11626472801037124 2316 30-31 -0.13542767976939984 2316 32-33 -0.12592243180908014 2316 34-35 -0.16898587754283056 2316 36-37 -0.1438483110759563 2316 38-39 -0.2591704782157578 2316 40-41 -0.3367600143927305 2316 42-43 -0.3708036693472607 2316 44-45 -0.31575203058153534 2316 46-47 -0.3579072704543407 2316 48-49 -0.2918950588442115 2316 50-51 -0.6599236626272855 2316 52-53 -0.5581644189822654 2316 54-55 -0.32201556107505525 2316 56-57 -0.27214249421074754 2316 58-59 -0.2504891361423276 2316 60-61 -0.178906095767573 2316 62-63 -0.0992468272251017 2316 64-65 -0.1191871195980454 2316 66-67 -0.15999513060867088 2316 68-69 -0.16864802854875904 2316 70-71 -0.30078476883318217 2316 72-73 -0.23107185634383143 2316 74-75 -0.2471282862277988 2316 76-77 -0.2827369995135669 2316 78-79 -0.2474847364006152 2316 80-81 -0.8094299873420212 2316 82-83 -0.6446139556502928 2316 84-85 -0.3847759877746668 2316 86-87 -0.31045764634824735 2316 88-89 -0.34193750147834123 2316 90-91 -0.3201232344204179 2316 92-93 -0.3854403598400893 2316 94-95 -0.32773353998449295 2316 96-97 -0.47891224021994816 2316 98-99 -0.5017187518184905 2316 100 -0.49590699886435274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 566.0 3 203.0 4 174.0 5 196.0 6 991.0 7 11269.0 8 33225.0 9 50527.0 10 61635.0 11 68948.0 12 74212.0 13 82732.0 14 100373.0 15 130287.0 16 165560.0 17 188485.0 18 205015.0 19 227252.0 20 260793.0 21 315938.0 22 392544.0 23 390288.0 24 467804.0 25 563804.0 26 630070.0 27 620893.0 28 660020.0 29 766711.0 30 897531.0 31 1071643.0 32 1355342.0 33 1779087.0 34 2445836.0 35 3794102.0 36 6247011.0 37 1.2453816E7 38 1.6209918E7 39 4938746.0 40 9519.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.20919219341974 31.16524880976022 27.090935955222623 24.534623041597417 2 21.280845756545418 28.046492996287153 30.880564917101584 19.792096330065846 3 20.103372519474146 28.62380475947032 27.585515549402533 23.687307171653 4 19.40844671995771 29.161125284649934 28.13642106519239 23.294006930199963 5 21.78414290837324 29.46833916116543 27.606584944968667 21.140932985492665 6 22.464084067535182 28.207844220521732 28.041610069188692 21.286461642754396 7 21.38053609771014 28.93399457017894 28.431474939522204 21.253994392588716 8 21.443435309691615 28.01588378336039 28.547004639759173 21.993676267188818 9 21.759039475466086 27.46627345074768 28.990165492008156 21.784521581778076 10-11 21.22731115846099 28.903358034559982 28.279442638110986 21.589888168868047 12-13 21.356338636619313 28.468757620416397 27.93794377240876 22.23695997055552 14-15 21.374846558697154 29.617594694631183 27.385541408509983 21.62201733816168 16-17 22.05088897920762 28.336554507472144 27.37211929420478 22.240437219115456 18-19 21.497944119636443 28.872848039146447 27.357686316512513 22.27152152470459 20-21 22.128654267604276 28.82898019988102 27.353626391387532 21.688739141127172 22-23 22.149660290038867 28.254983303787366 27.910383300448423 21.68497310572534 24-25 21.65330158056204 28.786218041013722 27.175662414097346 22.384817964326896 26-27 21.686802273493356 28.25424010880934 28.345732261562645 21.713225356134657 28-29 21.748048031664066 28.288632055691032 28.28388078960301 21.67943912304189 30-31 21.779458942154132 28.236673678547696 28.23845420772097 21.7454131715772 32-33 21.808362292126862 28.278794996927925 28.20134253029411 21.711500180651107 34-35 21.824292037166757 28.376274836099768 28.13257380576042 21.666859320973057 36-37 21.81009981237329 28.357788160785507 28.108479851443086 21.72363217539812 38-39 21.869742024006218 28.3430095177914 28.13698637664947 21.650262081552913 40-41 21.918464996769988 28.43347558966986 28.06673296922837 21.581326444331783 42-43 21.97295755931674 28.451102628561415 27.969282934001104 21.60665687812074 44-45 22.03593929028988 28.51326279501721 27.917063803756264 21.53373411093665 46-47 22.089295999680157 28.603602691431657 27.83350904480072 21.47359226408746 48-49 22.218849586760292 28.590173797196595 27.701026427583596 21.48995018845952 50-51 22.300617153269915 28.6038930514626 27.668204082545095 21.42728571272239 52-53 22.426129639220907 28.69263567345192 27.555259169392716 21.32597551793446 54-55 22.63840413388919 28.70057568843112 27.31777712082519 21.343243056854504 56-57 22.854108438957056 28.80432334505067 27.118782107972585 21.222786108019694 58-59 23.06923819239064 28.934147471958134 26.90554157091017 21.091072764741064 60-61 23.277289787135814 28.974786466607693 26.74231196180402 21.00561178445247 62-63 23.455740948904108 29.000386166352403 26.67161276242238 20.87226012232111 64-65 23.670784915169456 29.07191300651541 26.555937778266358 20.701364300048777 66-67 23.869390361306774 29.04866090701522 26.473792106749023 20.608156624928988 68-69 24.060646631099598 29.03267771103519 26.44760028450926 20.45907537335595 70-71 24.27624008210666 29.059935348743565 26.365199104865056 20.298625464284726 72-73 24.48197834572209 29.0339879012823 26.262255387988354 20.221778365007257 74-75 24.695284508098506 29.009699363192293 26.235915653346897 20.0591004753623 76-77 24.94265051973365 29.030063929916842 26.101645169000815 19.925640381348693 78-79 25.182965160762468 28.99819621666353 25.960148732345417 19.858689890228582 80-81 25.40699785275356 28.941035660751023 25.89195335306101 19.760013133434402 82-83 25.61824195428095 28.90888201162018 25.796711356960838 19.67616467713803 84-85 25.799586626131383 28.805696152076894 25.733244171202045 19.661473050589677 86-87 25.96908711079673 28.70738684281845 25.725438556338272 19.598087490046545 88-89 26.118898043605913 28.663640871155753 25.67983786481373 19.537623220424596 90-91 26.232034820264076 28.56554391143609 25.64896137839254 19.553459889907288 92-93 26.289179151589476 28.567812473859426 25.672241925334372 19.470766449216725 94-95 26.32385424029537 28.644069186329585 25.638897482293643 19.3931790910814 96-97 26.330185111921555 28.716153870088085 25.563798313662794 19.389862704327562 98-99 26.315271210982406 28.86012067057655 25.528962774752088 19.295645343688957 100 26.315935175835726 29.011773293813807 25.414337752895534 19.257953777454933 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 259334.0 1 152614.0 2 37617.0 3 27110.5 4 23604.0 5 21447.5 6 19915.5 7 19002.0 8 18464.5 9 18238.0 10 18635.5 11 21906.0 12 80667.0 13 90191.0 14 43320.5 15 45393.5 16 51394.0 17 58598.0 18 67467.5 19 78979.0 20 96947.5 21 127960.0 22 172746.0 23 225093.0 24 279665.5 25 342859.0 26 413069.0 27 492351.0 28 596747.0 29 707228.5 30 804727.5 31 910495.0 32 1025414.0 33 1129540.5 34 1226896.5 35 1341260.0 36 1469733.0 37 1567574.5 38 1653431.5 39 1736645.0 40 1795267.5 41 1859648.0 42 1930294.5 43 1964365.5 44 1980796.5 45 2022141.5 46 2065850.0 47 2097116.0 48 2117513.0 49 2128165.0 50 2121116.5 51 2094106.0 52 2041767.0 53 1950807.5 54 1833981.0 55 1698900.0 56 1541146.5 57 1374068.0 58 1201748.5 59 1010979.0 60 818519.0 61 649671.0 62 505327.5 63 381113.5 64 282883.0 65 207514.0 66 150479.0 67 112684.5 68 85882.0 69 65815.5 70 51831.0 71 43181.0 72 36549.5 73 27976.5 74 21132.5 75 17149.5 76 12614.5 77 7685.0 78 4667.0 79 3345.5 80 2516.5 81 1974.0 82 1503.0 83 1146.5 84 971.5 85 803.0 86 649.0 87 554.0 88 468.5 89 386.5 90 330.5 91 268.5 92 215.0 93 179.5 94 150.5 95 124.0 96 90.0 97 71.5 98 66.5 99 55.0 100 76.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05175552830848285 2 0.020200070514718258 3 0.007885830103084862 4 0.02899273640142523 5 0.030430149144489736 6 0.03268076644303946 7 0.021212674907902417 8 0.019483965010634256 9 0.015731780238629936 10-11 0.011849552094213268 12-13 0.009022409177968795 14-15 0.008394733167125188 16-17 0.009862489363752572 18-19 0.010267357729863017 20-21 0.4988090974736803 22-23 0.3984511591598061 24-25 0.3654825633858273 26-27 0.33796105100429374 28-29 0.33577198756868587 30-31 0.32971196641427036 32-33 0.31389002277076794 34-35 0.3086848200510096 36-37 0.3068815519535584 38-39 0.2957099593075215 40-41 0.2991387695601271 42-43 0.16038942684267904 44-45 0.09982302657535148 46-47 0.03357459788942034 48-49 0.01668283076887225 50-51 0.020850287376780005 52-53 0.026968394570872998 54-55 0.03408523486509283 56-57 0.034236085177091154 58-59 0.039763795460432086 60-61 0.03918293506365693 62-63 0.0466855706960334 64-65 0.04793485402700803 66-67 0.04729157281147495 68-69 0.049494507540140134 70-71 0.050318115565418355 72-73 0.05705956399127454 74-75 0.0585481271274879 76-77 0.06032798741790492 78-79 0.052115314972157024 80-81 0.05352585208492297 82-83 0.0643905423720667 84-85 0.05678733986502469 86-87 0.054861830997505835 88-89 0.057249427314996576 90-91 0.059127253612630895 92-93 0.05891224857024248 94-95 0.08064162914452996 96-97 0.054019149944273814 98-99 0.1507610155492687 100 0.09940341996036764 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5.7673066E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.62148651125584 #Duplication Level Percentage of deduplicated Percentage of total 1 75.58267977720922 32.97028846386102 2 11.74429422226924 10.246071440018751 3 4.181222063616215 5.471733655458001 4 2.1784541501138674 3.8010963329832546 5 1.3418983307894705 2.9267799968004806 6 0.9188808885886814 2.4049770172213165 7 0.6816653867329406 2.081468023079267 8 0.4914975603382685 1.7151883358888744 9 0.372996173023684 1.4643582777272408 >10 2.1924868794598753 17.801275240234226 >50 0.1814431160393493 5.4587024543580975 >100 0.12974517538636035 11.3693251126458 >500 0.0025698861675813936 0.6630653674019638 >1k 1.3879178974246352E-4 0.09926780387830561 >5k 7.885512152591244E-6 0.019506955499761214 >10k+ 1.9712963382573786E-5 1.5068955229436236 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 544698 0.9444581982168245 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 256628 0.44497027433915165 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.027239751741306766 1.7339116321646574E-5 0.0 0.0 1.7339116321646572E-6 2 0.02724148565293893 1.907302795381123E-5 0.0 0.0 3.4678232643293143E-6 3 0.027243219564571094 2.9476497746799175E-5 0.0 5.201734896493972E-6 8.669558160823287E-6 4 0.02724495347620326 3.814605590762246E-5 0.0 6.935646528658629E-6 1.0403469792987944E-5 5 0.02724495347620326 4.508170243628109E-5 0.0 8.669558160823287E-6 1.0403469792987944E-5 6 0.027246687387835426 5.548517222926903E-5 0.0 1.21373814251526E-5 1.21373814251526E-5 7 0.027250155211099754 6.242081875792766E-5 0.0 1.21373814251526E-5 1.3871293057317257E-5 8 0.027250155211099754 6.415473039009232E-5 0.0 1.5605204689481914E-5 2.2540851218140544E-5 9 0.027251889122731916 6.415473039009232E-5 0.0 1.7339116321646574E-5 2.6008674482469858E-5 10-11 0.027255356945996248 7.022342110266861E-5 0.0 2.0806939585975888E-5 2.7742586114634514E-5 12-13 0.02726402650415707 7.542515599916259E-5 0.0 2.2540851218140544E-5 3.727910009154013E-5 14-15 0.027284833443743047 9.536513976905614E-5 0.0 2.42747628503052E-5 4.768256988452807E-5 16-17 0.027303039515880777 1.0490165374596176E-4 0.0 2.42747628503052E-5 5.635212804535136E-5 18-19 0.027333382969443654 1.2224077006760834E-4 0.0 3.207736519504616E-5 6.242081875792766E-5 20-21 0.0273654603346387 1.395798863892549E-4 0.0 4.2480834988034105E-5 7.629211181524491E-5 22-23 0.027484233281441985 1.5605204689481914E-4 0.0 5.2884304781022046E-5 8.062689089565656E-5 24-25 0.02802694762230952 1.8899636790594765E-4 0.0 5.721908386143369E-5 8.842949324039752E-5 26-27 0.030257624937089354 2.063354842275942E-4 0.0 5.808603967751602E-5 9.276427232080917E-5 28-29 0.03791024392564807 2.1413808657233519E-4 0.0 6.155386294184533E-5 1.0230078629771477E-4 30-31 0.05833658990836381 2.2627546799748778E-4 0.0 6.588864202225698E-5 1.118373002746204E-4 32-33 0.1024958513563333 2.4708240758346365E-4 0.0 7.109037691875094E-5 1.335111956766786E-4 34-35 0.1830039693051866 2.670223913533572E-4 0.0 7.629211181524492E-5 1.3784597475709025E-4 36-37 0.30986734778414593 2.852284634910861E-4 0.0 8.149384671173889E-5 1.4911640036616053E-4 38-39 0.49158735552571453 2.964988891001564E-4 0.0 8.496166997606821E-5 1.5778595852698383E-4 40-41 0.7334714960359485 3.1643887287004994E-4 0.0 8.756253742431519E-5 1.6732247250388944E-4 42-43 1.0384726208244244 3.389797240881905E-4 8.669558160823286E-7 8.929644905647985E-5 1.7685898648079503E-4 44-45 1.4090606870111606 3.5025014969726075E-4 1.7339116321646572E-6 9.103036068864451E-5 1.84661588825536E-4 46-47 1.8497230579001989 3.6585535438674266E-4 1.7339116321646572E-6 9.449818395297383E-5 1.9506505861852395E-4 48-49 2.355627321772697 3.762588241797306E-4 1.7339116321646572E-6 9.883296303338546E-5 2.0546852841151189E-4 50-51 2.9249511375032498 3.8926316142096556E-4 1.7339116321646572E-6 1.031677421137971E-4 2.0980330749192352E-4 52-53 3.5524260145975246 4.005335870300358E-4 3.4678232643293143E-6 1.0490165374596176E-4 2.150050423884175E-4 54-55 4.233433679423251 4.1873965916776475E-4 3.4678232643293143E-6 1.1270425609070272E-4 2.1587199820449982E-4 56-57 4.967200807392484 4.369457313054936E-4 3.4678232643293143E-6 1.2224077006760834E-4 2.2887633544573476E-4 58-59 5.751546137671959 4.620874499718812E-4 3.4678232643293143E-6 1.248416375158553E-4 2.3841284942264037E-4 60-61 6.577377037662607 4.898300360865156E-4 3.4678232643293143E-6 1.3177728404451395E-4 2.4534849595129896E-4 62-63 7.4386907052938716 5.106369756724916E-4 3.4678232643293143E-6 1.4131379802141957E-4 2.522841424799576E-4 64-65 8.331419383876696 5.210404454654796E-4 3.4678232643293143E-6 1.4998335618224284E-4 2.652884797211926E-4 66-67 9.256033830419213 5.418473850514554E-4 3.4678232643293143E-6 1.543181352626545E-4 2.696232588016042E-4 68-69 10.204635210481094 5.496499873961964E-4 3.4678232643293143E-6 1.6125378179131312E-4 2.817606402267568E-4 70-71 11.177453267353602 5.678560595339253E-4 3.4678232643293143E-6 1.7165725158430105E-4 2.9129715420366247E-4 72-73 12.167511607584725 5.869290874877365E-4 3.4678232643293143E-6 1.7339116321646571E-4 2.9476497746799173E-4 74-75 13.177037440665977 5.981995130968068E-4 3.4678232643293143E-6 1.7685898648079503E-4 2.999667123644857E-4 76-77 14.198595753518635 6.103368945219594E-4 3.4678232643293143E-6 1.8032680974512435E-4 3.08636270525309E-4 78-79 15.235477510420548 6.302768782918529E-4 3.4678232643293143E-6 1.8119376556120667E-4 3.121040937896383E-4 80-81 16.281671239743 6.484829504295817E-4 3.4678232643293143E-6 1.8379463300945367E-4 3.2770929847912025E-4 82-83 17.33753932901712 6.692898900155577E-4 3.4678232643293143E-6 1.8552854464161833E-4 3.415805915364375E-4 84-85 18.40128717970361 6.918307412336982E-4 3.4678232643293143E-6 1.907302795381123E-4 3.459153706168491E-4 86-87 19.471503387733886 7.013672552106039E-4 3.4678232643293143E-6 1.941981028024416E-4 3.5198406132942543E-4 88 20.27710820853533 7.126376808196741E-4 3.4678232643293143E-6 1.941981028024416E-4 3.5371797296159007E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 91895 0.0 71.06153 8 GTGGTAT 96515 0.0 67.83449 6 GTATCAA 97295 0.0 66.90345 9 TGGTATC 98230 0.0 66.46135 7 AGTGGTA 98915 0.0 66.29238 5 GCAGTGG 116570 0.0 56.832417 3 AAGCAGT 119340 0.0 55.68509 1 CAGTGGT 119210 0.0 55.341522 4 AGCAGTG 132660 0.0 50.254547 2 CAACGCA 84045 0.0 41.040485 12-13 TCAACGC 82855 0.0 39.288208 12-13 ATCAACG 88115 0.0 39.286465 10-11 AACGCAG 86915 0.0 37.52078 14-15 ACGCAGA 92935 0.0 37.38974 14-15 AGAGTAC 95215 0.0 35.950035 18-19 GAGTACT 88015 0.0 34.368332 20-21 CGCAGAG 96750 0.0 33.675457 16-17 TATCAAC 99825 0.0 32.7672 10-11 AGTACTT 107275 0.0 30.182358 20-21 GTACTTT 102185 0.0 29.45584 22-23 >>END_MODULE