FastQCFastQC Report
Thu 26 May 2016
SRR1660669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57673066
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14924022.5876931876657987No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1643630.2849909175974796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA943820.16365004766696467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2055300.079.711988
GTGGTAT2095550.078.348696
AGTGGTA2116450.077.670525
TGGTATC2112750.077.544457
GTATCAA2117950.077.189899
AAGCAGT2249200.073.910311
GCAGTGG2257350.073.293523
CAGTGGT2294700.071.894114
AGCAGTG2415350.068.917932
CAACGCA1980000.043.67002512-13
ATCAACG2025000.042.7889110-11
ACGCAGA2074600.041.7029914-15
TCAACGC1972850.041.36447512-13
AGAGTAC2093200.040.93946518-19
AACGCAG2014400.040.40913414-15
GAGTACT2006650.038.9669820-21
CGCAGAG2113250.038.45159516-17
TATCAAC2143150.038.2112910-11
AGTACTT2195450.038.11594420-21
GCAGAGT2382800.036.3291416-17
Produced by FastQC (version 0.11.5)