##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660669_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57673066 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.498109689538616 34.0 31.0 34.0 31.0 34.0 2 32.69636585646409 34.0 31.0 34.0 31.0 34.0 3 32.71947917594671 34.0 31.0 34.0 31.0 34.0 4 36.18926656335559 37.0 37.0 37.0 35.0 37.0 5 36.09436084081259 37.0 35.0 37.0 35.0 37.0 6 36.07948438184299 37.0 36.0 37.0 35.0 37.0 7 36.048350958140496 37.0 35.0 37.0 35.0 37.0 8 36.05345647828052 37.0 35.0 37.0 35.0 37.0 9 37.77774314963592 39.0 38.0 39.0 35.0 39.0 10-11 37.73706590351898 39.0 37.5 39.0 35.0 39.0 12-13 37.76081879364624 39.0 38.0 39.0 35.0 39.0 14-15 39.2074071196423 41.0 39.0 41.0 36.0 41.0 16-17 39.18157516543337 41.0 39.0 41.0 36.0 41.0 18-19 39.16558387064076 41.0 39.0 41.0 36.0 41.0 20-21 39.152860817214055 40.5 39.0 41.0 36.0 41.0 22-23 39.065644767351195 40.0 39.0 41.0 35.5 41.0 24-25 39.057396792464615 40.0 39.0 41.0 35.0 41.0 26-27 38.996933655304545 40.0 39.0 41.0 35.0 41.0 28-29 38.90993127537212 40.0 38.0 41.0 35.0 41.0 30-31 38.85582988599913 40.0 38.0 41.0 35.0 41.0 32-33 38.77565150602536 40.0 38.0 41.0 35.0 41.0 34-35 38.703708131279164 40.0 38.0 41.0 35.0 41.0 36-37 38.599217978111305 40.0 38.0 41.0 34.0 41.0 38-39 38.383099487029185 40.0 38.0 41.0 34.0 41.0 40-41 38.29629633354329 40.0 38.0 41.0 34.0 41.0 42-43 38.20766449281542 40.0 38.0 41.0 34.0 41.0 44-45 38.11512919219519 40.0 37.0 41.0 33.0 41.0 46-47 38.0092859550765 40.0 37.0 41.0 33.0 41.0 48-49 37.88957621916616 40.0 37.0 41.0 33.0 41.0 50-51 37.803796541699384 40.0 37.0 41.0 33.0 41.0 52-53 37.68111580022467 40.0 37.0 41.0 33.0 41.0 54-55 37.3660200759918 40.0 36.0 41.0 32.0 41.0 56-57 37.08844710111302 39.5 36.0 41.0 31.0 41.0 58-59 36.86980505770232 39.0 35.5 41.0 31.0 41.0 60-61 36.66777209312923 39.0 35.0 41.0 31.0 41.0 62-63 36.44573411443047 39.0 35.0 40.5 31.0 41.0 64-65 36.186690395478536 38.0 35.0 40.0 30.0 41.0 66-67 35.907210672656106 37.5 35.0 40.0 30.0 41.0 68-69 35.597365163488966 37.0 35.0 40.0 30.0 41.0 70-71 35.278498207464814 36.5 34.5 39.0 29.0 41.0 72-73 34.931095123328454 36.0 34.0 39.0 29.0 40.5 74-75 34.57059146985527 35.5 34.0 38.5 29.0 40.0 76-77 33.70102388002053 35.0 32.5 37.0 28.0 39.0 78-79 33.99216545900299 35.0 34.0 37.0 29.0 39.0 80-81 33.8068191311348 35.0 34.0 36.5 29.0 39.0 82-83 33.55392072445048 35.0 34.0 36.0 29.0 37.5 84-85 33.31835561681427 35.0 34.0 36.0 29.0 37.0 86-87 33.11745349206855 35.0 34.0 35.0 29.0 37.0 88-89 32.952834829346514 35.0 34.0 35.0 28.5 36.0 90-91 32.8131676144979 35.0 33.0 35.0 28.0 36.0 92-93 32.70061831808977 35.0 33.0 35.0 27.0 36.0 94-95 32.595520506920856 35.0 33.0 35.0 27.0 35.5 96-97 32.50581110947006 35.0 33.0 35.0 27.0 35.0 98-99 32.43585876811197 35.0 33.0 35.0 27.0 35.0 100 32.39807205672055 35.0 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24173479759294736 1101 2 0.19808867672593777 1101 3 0.14764373140333475 1101 4 0.11973112672273345 1101 5 0.11502873659743074 1101 6 0.15244158240150796 1101 7 0.15614503042978356 1101 8 0.16121878688959868 1101 9 0.1879544496176777 1101 10-11 0.21548167487861747 1101 12-13 0.20600685657792184 1101 14-15 0.2734308211193479 1101 16-17 0.2838349899537107 1101 18-19 0.29714338991386313 1101 20-21 0.28867752835054716 1101 22-23 0.30278572882578914 1101 24-25 0.29843482134145205 1101 26-27 0.3084486607814583 1101 28-29 0.3751015145392742 1101 30-31 0.35901391204788524 1101 32-33 0.3512884237054408 1101 34-35 0.4009534672617221 1101 36-37 0.40407901377169253 1101 38-39 0.4121921236878521 1101 40-41 0.42303777625478034 1101 42-43 0.4404679946184231 1101 44-45 0.4370932161317427 1101 46-47 0.3951325588719996 1101 48-49 0.44105960842301073 1101 50-51 0.393726665886561 1101 52-53 0.3639977744959637 1101 54-55 0.32023269092668727 1101 56-57 0.2864859770841903 1101 58-59 0.2921257616091779 1101 60-61 0.2681707781260414 1101 62-63 0.24645455496760604 1101 64-65 0.27224966055323563 1101 66-67 0.22794668980645127 1101 68-69 0.2648120203250457 1101 70-71 0.2754038956362095 1101 72-73 0.30488944966601395 1101 74-75 0.3066494343922841 1101 76-77 0.6972641994358284 1101 78-79 0.3784789169590752 1101 80-81 0.2705524897722782 1101 82-83 0.26718460186525306 1101 84-85 0.2537564808497663 1101 86-87 0.24307494598954094 1101 88-89 0.24593248000235945 1101 90-91 0.23231161080177998 1101 92-93 0.21668454183136276 1101 94-95 0.23128647577376427 1101 96-97 0.21632331909862756 1101 98-99 0.21023895880360044 1101 100 0.21859893260813834 1102 1 0.17762767387645795 1102 2 0.14909221470416867 1102 3 0.10490155895427478 1102 4 0.09156927539884663 1102 5 0.09891965749378784 1102 6 0.12888221192998373 1102 7 0.13923452149734317 1102 8 0.11464217286665246 1102 9 0.1488246159890636 1102 10-11 0.1570826099163014 1102 12-13 0.14800226814781325 1102 14-15 0.19014400274421916 1102 16-17 0.21536993709042918 1102 18-19 0.23595449755473652 1102 20-21 0.2117308012760759 1102 22-23 0.21333640116969121 1102 24-25 0.21648926769654508 1102 26-27 0.25895963232039776 1102 28-29 0.28709344152840544 1102 30-31 0.28423176387033067 1102 32-33 0.25987580886009454 1102 34-35 0.28089755090962854 1102 36-37 0.3134919133305516 1102 38-39 0.3268001079421836 1102 40-41 0.3397909747133312 1102 42-43 0.3568352825622654 1102 44-45 0.36719880684639605 1102 46-47 0.381955861869713 1102 48-49 0.38231080341658696 1102 50-51 0.3135590477440786 1102 52-53 0.29846258271895465 1102 54-55 0.29093492846935476 1102 56-57 0.2595817449359288 1102 58-59 0.20873259826967683 1102 60-61 0.20546976073458012 1102 62-63 0.2044772172174376 1102 64-65 0.19066010428449687 1102 66-67 0.19046824046716893 1102 68-69 0.20237502412680186 1102 70-71 0.19941530863361123 1102 72-73 0.19495928835930698 1102 74-75 0.21006032488339343 1102 76-77 0.6057845037169116 1102 78-79 0.27810411288340475 1102 80-81 0.18747466405021385 1102 82-83 0.19831163245935102 1102 84-85 0.20083444148563956 1102 86-87 0.1757542335186244 1102 88-89 0.17894305704192703 1102 90-91 0.18912796116773478 1102 92-93 0.15803529271988026 1102 94-95 0.16293943299204727 1102 96-97 0.1946113622733847 1102 98-99 0.20224344963513374 1102 100 0.17400817618827347 1103 1 0.13347030245208202 1103 2 0.12389954799726155 1103 3 0.0679898310019027 1103 4 0.05263036538676147 1103 5 0.07576143752525155 1103 6 0.08186877681015403 1103 7 0.07101075157221004 1103 8 0.06362517271143275 1103 9 0.08250977427696427 1103 10-11 0.07958691066947665 1103 12-13 0.08969866416281747 1103 14-15 0.1509961776607156 1103 16-17 0.1398339351696123 1103 18-19 0.13732755298213561 1103 20-21 0.15450298722242906 1103 22-23 0.15579147624301726 1103 24-25 0.1610846418336891 1103 26-27 0.13154435469576953 1103 28-29 0.20313525620596806 1103 30-31 0.20413412986543733 1103 32-33 0.16694338915274187 1103 34-35 0.21145040853604513 1103 36-37 0.22023747485610556 1103 38-39 0.25951161903098097 1103 40-41 0.2738108093959397 1103 42-43 0.24784303287357545 1103 44-45 0.2899672200331196 1103 46-47 0.2846050466854919 1103 48-49 0.30036998198534093 1103 50-51 0.23216049559057694 1103 52-53 0.21893438939610377 1103 54-55 0.16175691912318513 1103 56-57 0.1513221845395023 1103 58-59 0.11727708593717523 1103 60-61 0.1291539311248684 1103 62-63 0.11398127099403155 1103 64-65 0.11771015841815569 1103 66-67 0.09886301853885726 1103 68-69 0.09941308213821998 1103 70-71 0.08301150333473117 1103 72-73 0.10624607568978917 1103 74-75 0.13063053624330934 1103 76-77 0.5216449164189143 1103 78-79 0.21512850481182966 1103 80-81 0.1484561351422542 1103 82-83 0.12737722116838768 1103 84-85 0.09973716155673884 1103 86-87 0.11381400272257025 1103 88-89 0.11800853434957048 1103 90-91 0.11750252596515764 1103 92-93 0.11318416374314211 1103 94-95 0.11383644336027032 1103 96-97 0.11182675807827991 1103 98-99 0.11675886532717072 1103 100 0.07912828690897555 1104 1 0.11815145298622554 1104 2 0.07704789639414855 1104 3 0.02974454580566288 1104 4 0.030196802544914192 1104 5 0.04010796260530469 1104 6 0.0716251309866891 1104 7 0.07801398601803555 1104 8 0.0716710908208924 1104 9 0.09038369766480514 1104 10-11 0.08428243282181569 1104 12-13 0.08597483768452463 1104 14-15 0.11259268347018292 1104 16-17 0.12342675909216183 1104 18-19 0.13829640434535406 1104 20-21 0.14112613247799288 1104 22-23 0.13961867764691505 1104 24-25 0.14519880135903662 1104 26-27 0.15173208733409638 1104 28-29 0.2178749928993895 1104 30-31 0.2001281460996296 1104 32-33 0.19369080580560194 1104 34-35 0.20693856132375998 1104 36-37 0.23835233944988943 1104 38-39 0.24574859379760028 1104 40-41 0.2734684768704696 1104 42-43 0.2288087294392369 1104 44-45 0.24470733458466043 1104 46-47 0.273307508240606 1104 48-49 0.2787734101401469 1104 50-51 0.22776755729695708 1104 52-53 0.17634866465014198 1104 54-55 0.1529861921110438 1104 56-57 0.12432667483096083 1104 58-59 0.08659589051450212 1104 60-61 0.06776351288144866 1104 62-63 0.053576462408408076 1104 64-65 0.07292609755710089 1104 66-67 0.07014621522804987 1104 68-69 0.030316231140766092 1104 70-71 0.04248259650615438 1104 72-73 0.06180662778606205 1104 74-75 0.07811574273347333 1104 76-77 0.4676936271857528 1104 78-79 0.17852068947662758 1104 80-81 0.1125898460561956 1104 82-83 0.11018442885853119 1104 84-85 0.10347011537883333 1104 86-87 0.08785056550100734 1104 88-89 0.09000736787130137 1104 90-91 0.07250799764248228 1104 92-93 0.07583602691139646 1104 94-95 0.06951067333083216 1104 96-97 0.12016682851418636 1104 98-99 0.09275651107924432 1104 100 0.07647168079371625 1105 1 0.06520023730134028 1105 2 0.05815115389489023 1105 3 -7.540236740979367E-4 1105 4 0.010469623531790262 1105 5 0.012128680342868847 1105 6 0.044023857775840725 1105 7 0.03492495058895173 1105 8 0.06226493042840531 1105 9 0.06349208684483187 1105 10-11 0.05152422589038963 1105 12-13 0.0500329127222372 1105 14-15 0.09177050599278402 1105 16-17 0.10169253744374629 1105 18-19 0.1094575121654131 1105 20-21 0.10810564056122018 1105 22-23 0.10245686741554039 1105 24-25 0.10315092359525124 1105 26-27 0.1068687912919728 1105 28-29 0.15568657667365926 1105 30-31 0.1290143621488795 1105 32-33 0.1585849950358167 1105 34-35 0.14115571443204544 1105 36-37 0.1638908185931598 1105 38-39 0.16488515294022932 1105 40-41 0.1710089242236208 1105 42-43 0.1917530567748429 1105 44-45 0.21564387531149976 1105 46-47 0.21339483913693869 1105 48-49 0.22437670568592694 1105 50-51 0.18303946092304102 1105 52-53 0.1618496973400383 1105 54-55 0.1153418168760112 1105 56-57 0.06714035406333352 1105 58-59 0.06375530692874065 1105 60-61 0.07524515170979384 1105 62-63 0.056481156712393954 1105 64-65 0.061075394052771514 1105 66-67 0.050564682657231685 1105 68-69 0.05272342702841826 1105 70-71 0.04330213746148104 1105 72-73 0.07169613104303352 1105 74-75 0.07446657978648119 1105 76-77 0.46210300326026044 1105 78-79 0.1625095078956491 1105 80-81 0.11433970004227945 1105 82-83 0.0922705067823415 1105 84-85 0.05893996733589546 1105 86-87 0.05997064285626408 1105 88-89 0.07664837563142157 1105 90-91 0.0712727338013508 1105 92-93 0.0428437948876379 1105 94-95 0.06777169992771803 1105 96-97 0.06159022342389875 1105 98-99 0.05195529908309027 1105 100 0.048645589479534124 1106 1 0.0339998023731809 1106 2 0.024807463716634004 1106 3 -0.03295606927440531 1106 4 -4.1700187767190755E-4 1106 5 -0.01645961990297451 1106 6 0.00595296367087883 1106 7 0.022322220394258352 1106 8 0.018775409622634243 1106 9 0.006693047087509285 1106 10-11 -0.008981673892321851 1106 12-13 0.023660531472131652 1106 14-15 0.02493502643413592 1106 16-17 0.04047647267361043 1106 18-19 0.035967935378742766 1106 20-21 0.06072628179623507 1106 22-23 0.04429716172270304 1106 24-25 0.07591061463342186 1106 26-27 0.0718994383055147 1106 28-29 0.07341108176751732 1106 30-31 0.084654600877613 1106 32-33 0.10811286350746485 1106 34-35 0.14447009685304835 1106 36-37 0.15354155136485304 1106 38-39 0.12988239204477026 1106 40-41 0.15669655163095086 1106 42-43 0.1685398030463432 1106 44-45 0.16774795948197863 1106 46-47 0.20607659898222153 1106 48-49 0.1985489484180718 1106 50-51 0.11289901377617184 1106 52-53 0.07793885612809248 1106 54-55 0.0427965949565845 1106 56-57 0.03580886318318477 1106 58-59 -0.006610479164947947 1106 60-61 -0.05867679634017264 1106 62-63 -0.06663686431843274 1106 64-65 -0.0447089997908634 1106 66-67 -0.04741486459693789 1106 68-69 -0.05260790295639595 1106 70-71 -0.025671259127712176 1106 72-73 -0.02446435507871314 1106 74-75 -0.03974704080032154 1106 76-77 0.4056857542503778 1106 78-79 0.0878208247593193 1106 80-81 0.0456326232920361 1106 82-83 0.024244181337053305 1106 84-85 0.03870275003288981 1106 86-87 0.03188975092640334 1106 88-89 0.04248202838180504 1106 90-91 0.001641617582045285 1106 92-93 -0.00791617470481043 1106 94-95 0.04395245089929034 1106 96-97 0.03575002365922586 1106 98-99 0.005804187193341193 1106 100 0.009217004344996838 1107 1 -0.010655591369392425 1107 2 -0.011306299848627077 1107 3 -0.06381890358770903 1107 4 -0.03995954965569126 1107 5 -0.08548953542242543 1107 6 -0.04502856187134796 1107 7 -0.05149918293523825 1107 8 -0.038331993766668404 1107 9 -0.039999200345334884 1107 10-11 -0.0395140846585349 1107 12-13 -0.026939950417727232 1107 14-15 -0.040858255444966574 1107 16-17 -0.02517006441234315 1107 18-19 -0.012723555999080816 1107 20-21 -0.013386825871904762 1107 22-23 -0.009495867919838474 1107 24-25 0.004405717968353429 1107 26-27 -0.009698169325055517 1107 28-29 0.0322234211072967 1107 30-31 -0.014960923073068955 1107 32-33 0.00442496866076425 1107 34-35 0.020281386699515735 1107 36-37 0.024902254624933562 1107 38-39 0.03971294304963635 1107 40-41 0.039283018875664766 1107 42-43 0.045971597339011794 1107 44-45 0.034308969354540864 1107 46-47 0.03829439840610149 1107 48-49 0.0595383715580482 1107 50-51 -0.012501890096444868 1107 52-53 0.0037212092746372605 1107 54-55 -0.022239773592829692 1107 56-57 -0.060930300965395645 1107 58-59 -0.07631409676848477 1107 60-61 -0.1047685828505891 1107 62-63 -0.10446664345673895 1107 64-65 -0.10481256803376482 1107 66-67 -0.10179960541198341 1107 68-69 -0.12455185665759672 1107 70-71 -0.1363408729116813 1107 72-73 -0.08465974554000155 1107 74-75 -0.09648475952430857 1107 76-77 0.26304660608952446 1107 78-79 0.00619802002111669 1107 80-81 -0.04478382154233884 1107 82-83 -0.03920168013094383 1107 84-85 -0.04609049163401835 1107 86-87 -0.01922183296233726 1107 88-89 -0.0029437955117188608 1107 90-91 -0.03430256329438208 1107 92-93 -0.037454024905684946 1107 94-95 -0.042707600561207926 1107 96-97 -0.03657545752889746 1107 98-99 -0.014363530466042107 1107 100 -0.027182223111438475 1108 1 0.005328973627307221 1108 2 -0.021179446627577647 1108 3 -0.078141276153751 1108 4 -0.02475666636997431 1108 5 -0.025638696036573094 1108 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-0.3328694430238457 2316 92-93 -0.2742088005907206 2316 94-95 -0.2645162847346185 2316 96-97 -0.30780472545175286 2316 98-99 -1.1034378508337817 2316 100 -0.6631491710442248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 10.0 7 285.0 8 565.0 9 364.0 10 232.0 11 204.0 12 357.0 13 1177.0 14 4116.0 15 10695.0 16 19856.0 17 29429.0 18 40512.0 19 55578.0 20 76685.0 21 109378.0 22 162835.0 23 253428.0 24 396695.0 25 548176.0 26 630426.0 27 630184.0 28 655194.0 29 752926.0 30 906484.0 31 1107106.0 32 1372602.0 33 1750108.0 34 2357892.0 35 3572755.0 36 6061737.0 37 1.2748841E7 38 1.8046837E7 39 5358346.0 40 11051.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.841469736225466 31.914577290528452 26.545482564372573 24.698470408873508 2 20.95090101884618 29.06186657931894 30.451952162550853 19.535280239284024 3 21.532546364983265 27.58242865591599 27.43733645121721 23.44768852788354 4 19.1339748642593 28.20039755543634 28.009805720095866 24.655821860208494 5 21.53027696091678 30.170547190608794 27.493799482742453 20.80537636573197 6 23.97574344663128 27.01320357173505 28.10547258474466 20.905580396889007 7 21.13588032819133 27.75345822178863 30.211964632016695 20.898696818003344 8 23.018543872801907 26.702875480904726 28.581872168890758 21.696708477402606 9 23.208480020812488 26.140782943636115 29.15718925017789 21.493547785373504 10-11 20.895975948287543 28.58535559735978 29.204935107836995 21.313733346515683 12-13 21.033033860207816 27.228955540529093 28.789222858378988 22.948787740884107 14-15 21.02890541661163 30.262900363230212 27.270195761744315 21.437998458413844 16-17 22.691651246701536 27.09139306032386 27.259958747468012 22.956996945506592 18-19 21.212718597987774 28.617635270249203 27.231952869724534 22.937693262038493 20-21 22.741233684908043 28.587674733558554 27.234464447384493 21.43662713414891 22-23 22.67919509854143 27.141351817679073 28.751265532139648 21.428187551639855 24-25 21.286367481223824 28.4751350534579 27.123790298050505 23.11470716726777 26-27 21.34145252937512 26.990913490533835 30.21567614081245 21.45195783927859 28-29 21.40492676363078 27.060031911051365 30.121934953088896 21.413106372228967 30-31 21.42233531936966 27.00692990452976 30.078004973810508 21.49272980229007 32-33 21.462500520890767 27.064683109999592 30.0098528342033 21.46296353490634 34-35 21.483916259612293 27.113117877184333 29.972065577177414 21.43090028602596 36-37 21.491078502678203 27.11791960765895 29.937165499834133 21.453836389828716 38-39 21.52099695334555 27.13538929983726 29.95887024724979 21.384743499567403 40-41 21.54732374387484 27.240921357195592 29.898469117245423 21.31328578168414 42-43 21.58928784203383 27.239326879437197 29.80116942857405 21.370215849954924 44-45 21.62694347613527 27.30963492193578 29.758983981123233 21.30443762080572 46-47 21.70202983687952 27.432194063850424 29.63947382124416 21.226302278025898 48-49 21.762109862139532 27.48703047254559 29.481850145605364 21.26900951970951 50-51 21.826950743531505 27.55504035064376 29.362047139370862 21.25596176645387 52-53 21.92682418503838 27.716696416352203 29.10529661295837 21.25118278565105 54-55 22.117702053599363 27.894091467288096 28.58204612950836 21.406160349604182 56-57 22.352514099570392 28.208517177740827 27.991437493916383 21.447531228772398 58-59 22.41459379339982 28.435764373096852 27.699459249320775 21.45018258418256 60-61 22.42602606482887 28.512699092934575 27.52893311478074 21.532341727455815 62-63 22.38595741664602 28.56808351664283 27.49086746706476 21.555091599646396 64-65 22.42779313793036 28.6305435560747 27.36343821103529 21.578225094959645 66-67 22.392573170649214 28.580846614421677 27.329942317521947 21.696637897407157 68-69 22.363134762685497 28.518944403528995 27.37891753802753 21.73900329575797 70-71 22.34678488197194 28.507680860437013 27.355868299327806 21.789665958263242 72-73 22.332431040475477 28.461623293207282 27.284014797809807 21.921930868507435 74-75 22.327372699717944 28.468876480983802 27.22066979867294 21.983081020625317 76-77 22.372052459538086 28.516838275143975 27.08178447106309 22.02932479425485 78-79 22.396943407490756 28.545889372956513 26.909728509594345 22.14743870995839 80-81 22.393142554194696 28.573509890906646 26.821320792484265 22.212026762414396 82-83 22.394061892920316 28.58401369201869 26.717021276577295 22.3049031384837 84-85 22.366150697185546 28.519466892167816 26.67099226315413 22.443390147492508 86-87 22.359152108821267 28.427839395412747 26.71402373598691 22.498984759779077 88-89 22.35785483255652 28.36085705108891 26.71512655400242 22.566161562352146 90-91 22.36542769226544 28.201393048159034 26.74936778226832 22.683811477307213 92-93 22.350859281869862 28.0947962141627 26.843337386416522 22.711007117550924 94-95 22.347074119504942 28.003594892627937 26.91515940170099 22.734171586166134 96-97 22.299631219385216 27.858726015817375 26.983888383309196 22.857754381488206 98-99 22.266610085449145 27.758800570897286 27.077745216345 22.896844127308572 100 22.235270507501408 27.742637360488036 27.061935788865455 22.9601563431451 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 208925.0 1 125742.5 2 28898.0 3 12855.5 4 9760.0 5 8690.5 6 8336.5 7 8322.0 8 8215.5 9 8356.5 10 9557.5 11 17468.5 12 168802.0 13 191588.0 14 64301.5 15 61504.5 16 68997.5 17 78807.5 18 90342.0 19 103903.5 20 123182.0 21 154581.0 22 198441.5 23 249062.0 24 300398.5 25 359369.0 26 426895.0 27 503770.5 28 606722.5 29 716852.5 30 811150.0 31 913764.0 32 1028487.0 33 1133908.5 34 1229691.5 35 1343255.5 36 1473931.0 37 1572382.0 38 1659074.0 39 1748240.0 40 1816821.5 41 1884900.5 42 1958491.0 43 2001020.5 44 2020928.5 45 2054427.0 46 2081357.0 47 2093658.0 48 2107294.0 49 2124057.0 50 2123628.0 51 2094416.5 52 2027394.5 53 1917634.5 54 1787883.5 55 1645679.5 56 1486792.0 57 1321416.0 58 1151633.0 59 966996.5 60 780789.0 61 618590.5 62 479601.5 63 358607.0 64 265091.0 65 194818.0 66 139790.5 67 103430.0 68 78113.0 69 59353.0 70 46750.0 71 38544.5 72 32328.5 73 24238.0 74 17598.5 75 14093.5 76 10029.5 77 5357.5 78 2711.5 79 1698.0 80 1124.0 81 720.5 82 451.0 83 258.0 84 162.5 85 118.5 86 74.5 87 41.5 88 27.0 89 21.0 90 13.0 91 7.5 92 5.0 93 3.5 94 2.5 95 1.0 96 0.0 97 1.0 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06360508040269612 2 0.02669010175391057 3 0.04555852813512637 4 0.0010507504490917822 5 9.536513976905614E-5 6 0.004131911419448378 7 1.7339116321646572E-6 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.2830946078018463E-4 20-21 9.432479278975735E-4 22-23 3.3377798919169654E-4 24-25 7.637880739685314E-4 26-27 0.006747517116568763 28-29 0.009137714301507742 30-31 0.011469825446769208 32-33 0.01287429386882258 34-35 0.01451284036121818 36-37 0.01940680594300293 38-39 0.017872294148537204 40-41 0.0202763626265335 42-43 0.021397336496727953 44-45 0.021782264879068508 46-47 0.02320580632907569 48-49 0.029567528107487816 50-51 0.008608871253697524 52-53 0.023638417281300773 54-55 0.057854562474622034 56-57 0.05831578296877784 58-59 0.048096107808799346 60-61 0.05551985046191232 62-63 0.036059293258312294 64-65 0.027642019239968965 66-67 0.030822013173358948 68-69 0.028129248408607234 70-71 0.020716776181103326 72-73 0.011841749491868527 74-75 0.00834358277397633 76-77 0.007615339888467174 78-79 0.006955586512428522 80-81 0.006251618389769672 82-83 0.0021145052354247995 84-85 0.00417352529862033 86-87 0.0034435485014790096 88-89 0.0023953989198354743 90-91 0.0011929312029292842 92-93 0.0023104372498594056 94-95 0.0031253757169767945 96-97 0.0038024682093370935 98-99 0.0024196736826857792 100 0.0016472160505564245 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5.7673066E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.00226381013308 #Duplication Level Percentage of deduplicated Percentage of total 1 73.08348043006316 29.235046643260848 2 12.728640375166448 10.183488604634391 3 4.654602618450567 5.585839256237874 4 2.4518348309800175 3.9231577491094285 5 1.5104406201376603 3.0210522078143853 6 1.008052711343746 2.419463429621548 7 0.7247202239683174 2.0293314711403556 8 0.5752603195369337 1.8409372049294301 9 0.43155289206837055 1.5536783372890361 >10 2.4752529453992578 18.334050338804516 >50 0.20638540639489333 5.717453735845452 >100 0.14552271149426985 11.708737946897282 >500 0.0038249907761104584 0.9097974331868057 >1k 3.6828947795394557E-4 0.2721247576787012 >5k 3.0317416798667386E-5 0.09518816283335899 >10k+ 3.0317325441810898E-5 3.170652720716615 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1492402 2.5876931876657987 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 164363 0.2849909175974796 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 94382 0.16365004766696467 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.02806335976658498 8.669558160823287E-6 0.0 1.7339116321646572E-6 1.7339116321646572E-6 2 0.02806335976658498 1.5605204689481914E-5 0.0 1.7339116321646572E-6 5.201734896493972E-6 3 0.02806335976658498 1.7339116321646574E-5 0.0 1.7339116321646572E-6 8.669558160823287E-6 4 0.02806335976658498 2.9476497746799175E-5 0.0 1.7339116321646572E-6 1.907302795381123E-5 5 0.02806335976658498 3.9879967539787115E-5 0.0 1.7339116321646572E-6 2.2540851218140544E-5 6 0.02806335976658498 5.2017348964939715E-5 0.0 3.4678232643293143E-6 3.467823264329315E-5 7 0.02806335976658498 5.721908386143369E-5 0.0 5.201734896493972E-6 4.508170243628109E-5 8 0.02806335976658498 6.93564652865863E-5 0.0 5.201734896493972E-6 5.028343733277506E-5 9 0.02806856150148147 7.629211181524492E-5 0.0 6.935646528658629E-6 5.028343733277506E-5 10-11 0.02807636410382621 9.189731650472684E-5 0.0 8.669558160823287E-6 6.0686907125763E-5 12-13 0.028084166706170956 1.0143383048163244E-4 0.0 1.1270425609070272E-5 6.762255365442164E-5 14-15 0.02809890495504435 1.0663556537812642E-4 0.0 1.907302795381123E-5 7.109037691875094E-5 16-17 0.028115377115549917 1.153051235389497E-4 0.0 2.1673895402058218E-5 7.542515599916259E-5 18-19 0.028143986657480635 1.369790189410079E-4 0.0 2.6008674482469858E-5 8.322775834390355E-5 20-21 0.028176930978491763 1.5518509107873682E-4 0.0 2.8609541930716845E-5 9.796600721730313E-5 22-23 0.028300905660191535 1.6472160505564244E-4 0.0 3.294432101112849E-5 1.0663556537812642E-4 24-25 0.02885749129411639 1.8379463300945365E-4 0.0 3.381127682721082E-5 1.1530512353894971E-4 26-27 0.031145387692757653 1.9853288188285327E-4 0.0 3.9013011723704785E-5 1.1703903517111437E-4 28-29 0.03894538917005037 2.1153721912408818E-4 0.0 4.508170243628109E-5 1.2570859333193766E-4 30-31 0.05965609666044111 2.280093796296524E-4 0.0 4.941648151669273E-5 1.387129305731726E-4 32-33 0.10429044989562372 2.418806726869697E-4 0.0 5.115039314885739E-5 1.4911640036616053E-4 34-35 0.18596549037292381 2.5921978900861624E-4 0.0 5.548517222926903E-5 1.5865291434306614E-4 36-37 0.3144570118744857 2.8002672859459216E-4 0.0 6.0686907125763E-5 1.7165725158430105E-4 38-39 0.49838602303543217 2.895632425714978E-4 0.0 6.762255365442164E-5 1.8119376556120667E-4 40-41 0.7429984734988773 3.0430149144489735E-4 0.0 7.802602344740957E-5 1.941981028024416E-4 42-43 1.0510824931693419 3.1817278450221463E-4 0.0 8.756253742431519E-5 2.0806939585975886E-4 44-45 1.4242575555112675 3.294432101112849E-4 0.0 9.70990514012208E-5 2.1327113075625284E-4 46-47 1.8671835480360972 3.4071363572035513E-4 0.0 1.0143383048163244E-4 2.210737331009938E-4 48-49 2.3757155549871407 3.545849287776724E-4 0.0 1.092364328263734E-4 2.3061024707789942E-4 50-51 2.947887320573524 3.684562218349897E-4 0.0 1.1703903517111435E-4 2.4101371687088735E-4 52-53 3.579103112014194 3.788596916279776E-4 0.0 1.274425049641023E-4 2.42747628503052E-4 54-55 4.2639609276191415 3.9533185213354186E-4 0.0 1.4044684220533723E-4 2.5315109829603994E-4 56-57 5.002418286553381 4.2307443824817636E-4 0.0 1.5085031199832519E-4 2.6268761227294556E-4 58-59 5.791791960566133 4.4388137783415223E-4 0.0 1.5865291434306612E-4 2.765589053302628E-4 60-61 6.6228124580718495 4.577526708914695E-4 0.0 1.6125378179131312E-4 2.878293309393331E-4 62-63 7.490301451981068 4.7422483139703377E-4 0.0 1.6558856087172476E-4 2.95631933284074E-4 64-65 8.388969644859873 4.95898726799092E-4 0.0 1.725242074003834E-4 3.0343453562881506E-4 66-67 9.319374489298003 5.063021965920799E-4 0.0 1.7945985392904204E-4 3.11237137973556E-4 68-69 10.2742838398777 5.158387105689855E-4 0.0 1.8639550045770068E-4 3.190397403182969E-4 70-71 11.254002865046225 5.33177826890632E-4 0.0 1.8986332372202994E-4 3.3204407755953186E-4 72-73 12.24987154315673 5.557186781087727E-4 0.0 1.9333114698635926E-4 3.363788566399435E-4 74-75 13.265472135641271 5.704569269821722E-4 0.0 1.9853288188285327E-4 3.459153706168491E-4 76-77 14.293582033596063 5.834612642234072E-4 0.0 2.0113374933110025E-4 3.545849287776724E-4 78-79 15.338145885984282 6.077360270737124E-4 0.0 2.0546852841151186E-4 3.606536194902487E-4 80-81 16.39315482204466 6.294099224757705E-4 0.0 2.0720244004367655E-4 3.69323177651072E-4 82-83 17.458826447687038 6.458820829813349E-4 0.0 2.1413808657233519E-4 3.771257799958129E-4 84-85 18.532559028507343 6.536846853260758E-4 0.0 2.2020677728491148E-4 3.8406142652447155E-4 86-87 19.612741760599306 6.692898900155578E-4 0.0 2.2367460054924078E-4 3.9533185213354186E-4 88 20.4264621547951 6.762255365442163E-4 0.0 2.2367460054924078E-4 3.987996753978712E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 205530 0.0 79.71198 8 GTGGTAT 209555 0.0 78.34869 6 AGTGGTA 211645 0.0 77.67052 5 TGGTATC 211275 0.0 77.54445 7 GTATCAA 211795 0.0 77.18989 9 AAGCAGT 224920 0.0 73.91031 1 GCAGTGG 225735 0.0 73.29352 3 CAGTGGT 229470 0.0 71.89411 4 AGCAGTG 241535 0.0 68.91793 2 CAACGCA 198000 0.0 43.670025 12-13 ATCAACG 202500 0.0 42.78891 10-11 ACGCAGA 207460 0.0 41.70299 14-15 TCAACGC 197285 0.0 41.364475 12-13 AGAGTAC 209320 0.0 40.939465 18-19 AACGCAG 201440 0.0 40.409134 14-15 GAGTACT 200665 0.0 38.96698 20-21 CGCAGAG 211325 0.0 38.451595 16-17 TATCAAC 214315 0.0 38.21129 10-11 AGTACTT 219545 0.0 38.115944 20-21 GCAGAGT 238280 0.0 36.32914 16-17 >>END_MODULE