FastQCFastQC Report
Thu 26 May 2016
SRR1660668_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660668_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57680567
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2603170.45130797691361113No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1185010.20544354218986785No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC750180.1300576674289627Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA368800.051.4384848
GTATCAA421850.044.9590079
GTGGTAT425950.044.526216
TGGTATC429800.044.0182347
AGTGGTA440950.043.0540735
CAACGCA286550.034.99997312-13
TCAACGC273050.034.89304412-13
ATCAACG322150.031.29951110-11
AACGCAG317950.030.10391814-15
CAGTGGT644450.029.8807374
GCAGTGG659500.029.362373
AAGCAGT699650.027.8380551
ACGCAGA361050.027.80667314-15
AGAGTAC405950.024.34096518-19
CGCAGAG395300.024.1774816-17
GAGTACT349700.023.48430420-21
AGCAGTG836850.023.3190172
TATCAAC434250.022.1669210-11
AGTACTT522550.016.57492820-21
GTACTTT512050.016.06924422-23
Produced by FastQC (version 0.11.5)