##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660668_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57680567 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.395505560824326 33.0 31.0 34.0 28.0 34.0 2 31.528493643275038 34.0 31.0 34.0 28.0 34.0 3 31.50157064163395 34.0 31.0 34.0 27.0 34.0 4 34.9402401158782 37.0 35.0 37.0 32.0 37.0 5 34.904445235429115 37.0 35.0 37.0 32.0 37.0 6 34.867736581022164 37.0 35.0 37.0 31.0 37.0 7 34.884715835057584 37.0 35.0 37.0 31.0 37.0 8 34.78572105575869 37.0 35.0 37.0 31.0 37.0 9 36.229355980498596 39.0 37.0 39.0 30.0 39.0 10-11 36.369306728555564 39.0 37.0 39.0 31.0 39.0 12-13 36.367299527412754 39.0 37.0 39.0 31.0 39.0 14-15 37.64529720208888 40.0 38.0 41.0 31.0 41.0 16-17 37.61561581910247 40.0 38.0 41.0 31.0 41.0 18-19 37.633276307772775 40.0 38.0 41.0 31.0 41.0 20-21 37.38542394182775 40.0 38.0 41.0 31.0 41.0 22-23 37.4433334887294 40.0 38.0 41.0 31.0 41.0 24-25 37.46908657295272 40.0 37.5 41.0 31.0 41.0 26-27 37.44479956308335 40.0 37.0 41.0 31.0 41.0 28-29 37.406618593052315 40.0 37.0 41.0 31.0 41.0 30-31 37.32791024748422 40.0 37.0 41.0 30.0 41.0 32-33 37.26711873342022 40.0 37.0 41.0 30.0 41.0 34-35 37.19388275950894 40.0 37.0 41.0 30.0 41.0 36-37 37.13354057355227 40.0 37.0 41.0 30.0 41.0 38-39 37.04899807937048 40.0 37.0 41.0 30.0 41.0 40-41 36.9689350747887 40.0 36.0 41.0 30.0 41.0 42-43 36.81600433990186 40.0 36.0 41.0 30.0 41.0 44-45 36.68538547826688 40.0 36.0 41.0 29.5 41.0 46-47 36.64312863810787 40.0 35.0 41.0 29.0 41.0 48-49 36.606552177269684 39.0 35.0 41.0 29.0 41.0 50-51 36.48690863250356 39.0 35.0 41.0 29.0 41.0 52-53 36.3431631141906 39.0 35.0 41.0 28.5 41.0 54-55 36.178670721111324 39.0 35.0 40.5 28.0 41.0 56-57 35.99003482403354 39.0 35.0 40.0 28.0 41.0 58-59 35.78646906331555 38.0 34.0 40.0 28.0 41.0 60-61 35.539413577539904 38.0 34.0 40.0 27.5 41.0 62-63 35.24845941614964 37.5 34.0 40.0 27.0 41.0 64-65 34.936064628144166 37.0 33.5 40.0 26.0 41.0 66-67 34.76037123560176 36.5 33.0 39.5 26.0 41.0 68-69 34.473886092347186 36.0 33.0 39.0 26.0 41.0 70-71 34.13883922118866 35.5 33.0 39.0 26.0 40.5 72-73 33.79215056953237 35.0 33.0 38.5 26.0 40.0 74-75 33.60194149097043 35.0 33.0 37.5 26.0 40.0 76-77 33.3137142705272 35.0 32.0 37.0 26.0 39.0 78-79 33.0143815593907 35.0 32.0 36.5 25.5 39.0 80-81 32.72493443935806 35.0 32.0 36.0 25.0 38.5 82-83 32.45413048730953 35.0 32.0 36.0 25.0 37.0 84-85 32.21870607651967 35.0 32.0 35.0 25.0 37.0 86-87 32.01241197923731 35.0 31.0 35.0 24.5 36.5 88-89 31.817425338415973 35.0 31.0 35.0 24.0 36.0 90-91 31.67597542860492 34.0 31.0 35.0 24.0 36.0 92-93 31.677943699825278 34.0 31.0 35.0 24.0 36.0 94-95 31.6437450242124 34.5 31.0 35.0 24.0 35.0 96-97 31.74334101986203 35.0 32.0 35.0 24.0 35.0 98-99 31.706864610051422 35.0 32.0 35.0 24.0 35.0 100 31.6455663828686 35.0 32.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.021449687823729136 1101 2 -0.013511156530150004 1101 3 0.03546960328483806 1101 4 0.06908898108271444 1101 5 0.05313599556540538 1101 6 0.055125397245028296 1101 7 0.060722616835896304 1101 8 0.0813999947910915 1101 9 0.09427325917021534 1101 10-11 0.06926945100872217 1101 12-13 9.840633381514863E-4 1101 14-15 0.06076535473850697 1101 16-17 0.047722994702901644 1101 18-19 0.11396145404395952 1101 20-21 -0.08510613672292777 1101 22-23 0.016927710972673538 1101 24-25 0.07175443319995622 1101 26-27 0.10170488638771502 1101 28-29 0.061667267042082585 1101 30-31 0.12287144186868204 1101 32-33 0.11969916047783613 1101 34-35 0.13804126813449358 1101 36-37 0.14990018811227657 1101 38-39 0.24971746727602806 1101 40-41 0.3243200483916411 1101 42-43 0.373979788766718 1101 44-45 0.35523305475388156 1101 46-47 0.4116990466571835 1101 48-49 0.3419242351155489 1101 50-51 0.6357304405861015 1101 52-53 0.590638439107984 1101 54-55 0.41131636322956666 1101 56-57 0.31687160335219033 1101 58-59 0.34329166700507585 1101 60-61 0.24406592054436516 1101 62-63 0.23462381214881134 1101 64-65 0.2934468135746471 1101 66-67 0.2730594940143831 1101 68-69 0.32889632060641105 1101 70-71 0.3148406009870399 1101 72-73 0.30747626450526155 1101 74-75 0.47152635919638897 1101 76-77 0.43153292999298287 1101 78-79 0.42013920550970596 1101 80-81 0.4252386712646725 1101 82-83 0.33306285695147153 1101 84-85 0.3799739464159231 1101 86-87 0.3911642686007468 1101 88-89 0.4169453659941702 1101 90-91 0.36676358289518873 1101 92-93 0.3424739462565469 1101 94-95 0.3691453576716235 1101 96-97 0.5044565531365741 1101 98-99 0.48297522803034454 1101 100 0.45289799696127275 1102 1 -0.04704031014241039 1102 2 0.012198313868161392 1102 3 -0.04110366593716819 1102 4 0.035037615922099974 1102 5 0.008634727539636344 1102 6 0.011625403621181363 1102 7 0.025050551854327807 1102 8 0.039736534743383345 1102 9 0.04157144590307382 1102 10-11 0.02728147343218268 1102 12-13 -0.020208844501183876 1102 14-15 0.0052893866373366905 1102 16-17 -0.024557752830595803 1102 18-19 3.241276372989432E-5 1102 20-21 -0.15504758227045556 1102 22-23 -0.01687597758935766 1102 24-25 -0.051167209314030515 1102 26-27 0.012798746816308437 1102 28-29 0.0322615668324957 1102 30-31 0.05044156092624519 1102 32-33 0.05057051830871728 1102 34-35 0.08711585531384713 1102 36-37 0.11617087116937341 1102 38-39 0.23595314547318935 1102 40-41 0.26330545183953546 1102 42-43 0.3015605859634576 1102 44-45 0.28798474110728023 1102 46-47 0.3298854590497555 1102 48-49 0.30950723431367777 1102 50-51 0.6050063767611675 1102 52-53 0.5632408911390385 1102 54-55 0.3436471447373606 1102 56-57 0.31498097607066455 1102 58-59 0.2209337242704521 1102 60-61 0.1654394308566438 1102 62-63 0.19568484024539146 1102 64-65 0.2023421160714065 1102 66-67 0.22131711970573065 1102 68-69 0.31228812451066545 1102 70-71 0.27395722213443463 1102 72-73 0.2552657907128193 1102 74-75 0.38634291321672265 1102 76-77 0.35812098122012515 1102 78-79 0.30589293328373657 1102 80-81 0.2963297966755576 1102 82-83 0.26594993030526126 1102 84-85 0.3065692273619405 1102 86-87 0.344567454126377 1102 88-89 0.35771872539794813 1102 90-91 0.3325204662285728 1102 92-93 0.27534869258039407 1102 94-95 0.2512862218181766 1102 96-97 0.3892195133701257 1102 98-99 0.43567352979559715 1102 100 0.44277543685120335 1103 1 -0.15917568759998701 1103 2 -0.10835824014949935 1103 3 -0.07614361088762678 1103 4 -0.059094857833486 1103 5 -0.07426617610430952 1103 6 -0.05815643575976992 1103 7 -0.04831026329419075 1103 8 -0.03509305506304372 1103 9 -0.013468063348824444 1103 10-11 -0.05571965789150113 1103 12-13 -0.10575116185597011 1103 14-15 -0.06340784503056796 1103 16-17 -0.09876857155872898 1103 18-19 -0.021831214214522277 1103 20-21 -0.22216396363458557 1103 22-23 -0.09379727227127432 1103 24-25 -0.0901094475879205 1103 26-27 -0.06561532413824267 1103 28-29 -0.056921501851142864 1103 30-31 -0.026855848997946907 1103 32-33 -0.045146712327110095 1103 34-35 0.028181865891447444 1103 36-37 0.028140084486850014 1103 38-39 0.11562972893226231 1103 40-41 0.20234326861684337 1103 42-43 0.21106838220087099 1103 44-45 0.18883710812085042 1103 46-47 0.24571054285267024 1103 48-49 0.21253653474712308 1103 50-51 0.5021235570906555 1103 52-53 0.41614317427782765 1103 54-55 0.2648517521617535 1103 56-57 0.1493682910783889 1103 58-59 0.14153000547402428 1103 60-61 0.03005785699214414 1103 62-63 0.07287506107650188 1103 64-65 0.14515280923743035 1103 66-67 0.18119621574129496 1103 68-69 0.23151557114033494 1103 70-71 0.20721714486440135 1103 72-73 0.19759764987273343 1103 74-75 0.3285931121374759 1103 76-77 0.2661090390451335 1103 78-79 0.23303424983904364 1103 80-81 0.2689479742618701 1103 82-83 0.22534749952626498 1103 84-85 0.28782924612963967 1103 86-87 0.24067939550397455 1103 88-89 0.2747833845696057 1103 90-91 0.28957979390672506 1103 92-93 0.1905657543784649 1103 94-95 0.20264104515210235 1103 96-97 0.34041644371918167 1103 98-99 0.3532437960983579 1103 100 0.3267384341395818 1104 1 -0.2221942651045623 1104 2 -0.20173545515435265 1104 3 -0.1964822059823632 1104 4 -0.11393932120838457 1104 5 -0.15370876958257895 1104 6 -0.13391597074778616 1104 7 -0.1526569024238995 1104 8 -0.1438069322926978 1104 9 -0.1328837215200025 1104 10-11 -0.16929063190596594 1104 12-13 -0.18830912865634986 1104 14-15 -0.17582628564525038 1104 16-17 -0.18319463147825132 1104 18-19 -0.16046164248716366 1104 20-21 -0.34304784560782764 1104 22-23 -0.1880776918563427 1104 24-25 -0.20575583897468164 1104 26-27 -0.21864257570957335 1104 28-29 -0.18504874536474603 1104 30-31 -0.11607162034815133 1104 32-33 -0.1367788948141211 1104 34-35 -0.12335203129915584 1104 36-37 -0.1039769701994544 1104 38-39 0.013935940952585213 1104 40-41 0.0885168207685183 1104 42-43 0.052953454310909365 1104 44-45 0.06400849339532044 1104 46-47 0.12390758353249254 1104 48-49 0.0787953399223511 1104 50-51 0.35372248328334877 1104 52-53 0.25381893430098046 1104 54-55 0.08939497660897189 1104 56-57 0.11007178073764123 1104 58-59 0.05477416755158515 1104 60-61 -0.06384975826065187 1104 62-63 0.013538548383593252 1104 64-65 0.03420459940773668 1104 66-67 0.08528206703092422 1104 68-69 0.11834179526312738 1104 70-71 0.0937551641764287 1104 72-73 0.08715627323028485 1104 74-75 0.24217076424774575 1104 76-77 0.18776131597748247 1104 78-79 0.17731105365315614 1104 80-81 0.23998063494072852 1104 82-83 0.14282086096203273 1104 84-85 0.18489659500728095 1104 86-87 0.17011519437875933 1104 88-89 0.19299191977485108 1104 90-91 0.2157553492639508 1104 92-93 0.11348200656086505 1104 94-95 0.1617301786088312 1104 96-97 0.26118890773168246 1104 98-99 0.2960111905919014 1104 100 0.26643298553963746 1105 1 -0.3930247818879735 1105 2 -0.3071657234785654 1105 3 -0.29593534984871184 1105 4 -0.22865946818128435 1105 5 -0.29107005032586386 1105 6 -0.27002811655246006 1105 7 -0.26822731237923136 1105 8 -0.23624669904874196 1105 9 -0.260686353023992 1105 10-11 -0.3136436557051425 1105 12-13 -0.3187428765909601 1105 14-15 -0.34561229900823776 1105 16-17 -0.3473237688646691 1105 18-19 -0.30821563315885925 1105 20-21 -0.47095958896259305 1105 22-23 -0.30497015632511904 1105 24-25 -0.3168906014206172 1105 26-27 -0.2504139653067554 1105 28-29 -0.32318611260830465 1105 30-31 -0.24663994242266085 1105 32-33 -0.2743423624525576 1105 34-35 -0.24450173713908185 1105 36-37 -0.23313503301163507 1105 38-39 -0.08858650865707318 1105 40-41 -0.055259517031245764 1105 42-43 -0.08090869518670019 1105 44-45 -0.033822682094779566 1105 46-47 0.042267355915605265 1105 48-49 -0.031085866594189326 1105 50-51 0.2725517235495758 1105 52-53 0.2240463160002193 1105 54-55 0.01204350823965683 1105 56-57 0.0027405753977589598 1105 58-59 -0.08114801072703415 1105 60-61 -0.15347765125550694 1105 62-63 -0.14100630736002984 1105 64-65 -0.102251241135626 1105 66-67 -0.025502710851228017 1105 68-69 0.011765404413054625 1105 70-71 -0.009079491602768996 1105 72-73 -0.003174749670300514 1105 74-75 0.11905136697545515 1105 76-77 0.12351347766568921 1105 78-79 0.1021654195001318 1105 80-81 0.1431201861136273 1105 82-83 0.06138191700343043 1105 84-85 0.16247749951111956 1105 86-87 0.17481948907971656 1105 88-89 0.16741126431494635 1105 90-91 0.1561414062832185 1105 92-93 0.05661941892881117 1105 94-95 0.0568537363924122 1105 96-97 0.22108119274917115 1105 98-99 0.2150174908451028 1105 100 0.21213639292131958 1106 1 -0.37357371237790815 1106 2 -0.3521089362164673 1106 3 -0.31212113454464685 1106 4 -0.22405545618532585 1106 5 -0.27188282049444723 1106 6 -0.26598709997412584 1106 7 -0.2479404172058821 1106 8 -0.21291495612041444 1106 9 -0.20762558107294637 1106 10-11 -0.2685162140252402 1106 12-13 -0.31490262999305685 1106 14-15 -0.3275720711515504 1106 16-17 -0.3565734864830148 1106 18-19 -0.2750232963644237 1106 20-21 -0.42661085954107136 1106 22-23 -0.3293739005899923 1106 24-25 -0.31555711304524436 1106 26-27 -0.31000853152210794 1106 28-29 -0.33497687288205924 1106 30-31 -0.27856625423994075 1106 32-33 -0.2844543609812362 1106 34-35 -0.2668379416593254 1106 36-37 -0.24668581159063763 1106 38-39 -0.12426783424348287 1106 40-41 -0.11274401970784709 1106 42-43 -0.07134675597117734 1106 44-45 -0.043627303876547785 1106 46-47 -0.021152204859994583 1106 48-49 -0.05139486642355706 1106 50-51 0.24435237991765035 1106 52-53 0.19028218529080476 1106 54-55 0.007638092849305167 1106 56-57 -0.0368659526082169 1106 58-59 -0.05954512515043575 1106 60-61 -0.14698728180541565 1106 62-63 -0.1053066382682104 1106 64-65 -0.08023306767929483 1106 66-67 -0.04717165131526002 1106 68-69 0.016534694478686163 1106 70-71 0.025384392384729892 1106 72-73 0.023192567036552703 1106 74-75 0.15837982891704883 1106 76-77 0.15023587268458272 1106 78-79 0.1360337172646311 1106 80-81 0.15386436269495363 1106 82-83 0.10100585377583116 1106 84-85 0.19806894332952396 1106 86-87 0.18568840593677294 1106 88-89 0.1993911255780283 1106 90-91 0.20246613206546016 1106 92-93 0.11562425405779209 1106 94-95 0.0587907814545261 1106 96-97 0.23617540677195237 1106 98-99 0.274139739859649 1106 100 0.2362501256699332 1107 1 -0.36650856489044514 1107 2 -0.29340698664831066 1107 3 -0.2865105497822711 1107 4 -0.23466481092437874 1107 5 -0.26206155348245375 1107 6 -0.25397192838563853 1107 7 -0.24739589857172462 1107 8 -0.2442229350198204 1107 9 -0.25460174635151134 1107 10-11 -0.2890318490451733 1107 12-13 -0.3470036081499046 1107 14-15 -0.38647912139597196 1107 16-17 -0.40956388185804116 1107 18-19 -0.3617632068555636 1107 20-21 -0.5194218084542399 1107 22-23 -0.36576523638803593 1107 24-25 -0.3640338933200411 1107 26-27 -0.3360292052249818 1107 28-29 -0.3404258217528664 1107 30-31 -0.2741745574640575 1107 32-33 -0.2981225658723332 1107 34-35 -0.26291692990421467 1107 36-37 -0.22127087338401452 1107 38-39 -0.15692147611656537 1107 40-41 -0.13885112868143779 1107 42-43 -0.13598278896006377 1107 44-45 -0.10096012392566678 1107 46-47 -0.06072406067007563 1107 48-49 -0.09853099622601036 1107 50-51 0.22016585142514344 1107 52-53 0.11653551582146093 1107 54-55 -0.01495667440933346 1107 56-57 -0.07792501143944008 1107 58-59 -0.1374154015930813 1107 60-61 -0.21773319126246093 1107 62-63 -0.1608703737574757 1107 64-65 -0.14069904857297644 1107 66-67 -0.0930342375256501 1107 68-69 -0.03327219758896405 1107 70-71 -0.023669000951329622 1107 72-73 -0.054593349381370615 1107 74-75 0.061322015342042846 1107 76-77 0.07406873270964809 1107 78-79 0.020183293589241202 1107 80-81 0.06768782380340355 1107 82-83 -0.029221539117081363 1107 84-85 0.08206038777027658 1107 86-87 0.09728645139871617 1107 88-89 0.10485999485298336 1107 90-91 0.14695094492648053 1107 92-93 0.04724400173245158 1107 94-95 0.046565484627052456 1107 96-97 0.15568585111961752 1107 98-99 0.18940042460832984 1107 100 0.1681244374505404 1108 1 -0.3502576173761369 1108 2 -0.3058335154247729 1108 3 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0.7554864787477449 2316 1 0.2370213906661469 2316 2 0.8269182901023058 2316 3 -0.45993972028864505 2316 4 0.2409190191408257 2316 5 0.061308709095264646 2316 6 0.7674667665412827 2316 7 0.912014205596158 2316 8 1.1421379839438686 2316 9 1.5371040464558874 2316 10-11 -0.05239549177150593 2316 12-13 0.5994656343291211 2316 14-15 1.3423738070863038 2316 16-17 1.4322511968219587 2316 18-19 1.3831115117278472 2316 20-21 1.6353810103776283 2316 22-23 0.7666981357117706 2316 24-25 1.3886698202124563 2316 26-27 1.364924111699672 2316 28-29 1.1502932632495302 2316 30-31 1.0397299737804744 2316 32-33 1.8013487208867929 2316 34-35 2.0698395966276806 2316 36-37 1.8704913485530597 2316 38-39 -0.2937646672932033 2316 40-41 0.5323802949073979 2316 42-43 1.85474395115682 2316 44-45 2.298421051200023 2316 46-47 -0.4715445285506732 2316 48-49 -0.44647782161357696 2316 50-51 -0.36767257754122795 2316 52-53 0.8812504685201432 2316 54-55 0.08879710211381564 2316 56-57 0.777820188439243 2316 58-59 1.5209816757571133 2316 60-61 0.7315341778277897 2316 62-63 0.6263061429758778 2316 64-65 1.1691110900064672 2316 66-67 2.455883037661863 2316 68-69 -0.19630681620386525 2316 70-71 0.18881911539475027 2316 72-73 0.4603439661668247 2316 74-75 0.7255770779244273 2316 76-77 0.1945022746509295 2316 78-79 -0.5249844336145557 2316 80-81 0.655111053940935 2316 82-83 -0.6233572110757422 2316 84-85 0.6780923409472237 2316 86-87 1.614408390811036 2316 88-89 1.8135726816377762 2316 90-91 1.3314661793333755 2316 92-93 2.130637725413436 2316 94-95 1.6291608892253713 2316 96-97 0.78546993314745 2316 98-99 1.0038736215007908 2316 100 -0.9141563783951092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 3.0 5 42.0 6 555.0 7 8461.0 8 34820.0 9 61449.0 10 80072.0 11 94817.0 12 107863.0 13 121099.0 14 140380.0 15 171437.0 16 207500.0 17 228875.0 18 249458.0 19 280666.0 20 323338.0 21 392286.0 22 482165.0 23 433508.0 24 481407.0 25 555816.0 26 641888.0 27 706796.0 28 782219.0 29 917519.0 30 1081440.0 31 1281535.0 32 1616544.0 33 2100090.0 34 2851524.0 35 4259442.0 36 6735955.0 37 1.2227718E7 38 1.4194229E7 39 3823073.0 40 4576.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.650652851605848 30.111785033196732 27.349333451691336 23.88822866350608 2 21.97317411650463 27.63098420837603 29.230160823710605 21.165680851408734 3 19.63701909523916 28.44386779443403 27.845145627802516 24.073967482524296 4 20.026313648306182 28.729519797850212 28.30188270344606 22.942283850397548 5 21.75189312194056 28.42646311503697 27.856430870706493 21.965212892315975 6 22.16811712582775 28.032451545499228 27.912430226175992 21.887001102497027 7 21.913130562941614 28.68917544489229 27.598416450066726 21.799277542099368 8 21.33042214279729 28.286588171923675 28.07244808098414 22.31054160429489 9 21.591810147609376 27.996132962696024 28.27266496674684 22.139391922947755 10-11 21.669709795017926 28.711554716290955 27.66314763692342 21.955587851767703 12-13 21.752860248439397 28.57803280999292 27.482680561458857 22.186426380108827 14-15 21.766963274452305 28.89977769131703 27.422113831605856 21.91114520262481 16-17 22.01115111775407 28.452246063462844 27.36201505180012 22.174587766982963 18-19 21.873379865708326 28.53232230654034 27.403954633483078 22.190343194268255 20-21 22.028886628359135 28.547790852181315 27.3879720769167 22.035350442542853 22-23 22.24027618206586 28.29675966708528 27.439561080247316 22.023403070601542 24-25 22.079792040670654 28.49410232875519 27.158782511133655 22.267323119440498 26-27 22.035835565744648 28.434151244157768 27.46047024585614 22.069542944241444 28-29 22.15466717847153 28.388633941481782 27.414218454635492 22.042480425411192 30-31 22.21844405384979 28.269074704494816 27.42840988044754 22.08407136120785 32-33 22.223721817224146 28.315990408259385 27.382259523435735 22.078028251080735 34-35 22.264148741358873 28.46068663397943 27.264778224137416 22.01038640052428 36-37 22.18000386611367 28.42952740614299 27.323994826775856 22.06647390096748 38-39 22.247570036373602 28.355281206977782 27.310273223408977 22.086875533239642 40-41 22.393907455260425 28.433717673678448 27.158986317846413 22.013388553214718 42-43 22.457150371176585 28.446575532999397 27.145718870595548 21.95055522522847 44-45 22.588783540506025 28.487550202434765 27.03370501828945 21.889961238769757 46-47 22.50880371743125 28.56023673566433 27.025132070394985 21.905827476509437 48-49 22.695583270064642 28.534760553304967 26.848642432158886 21.921013744471505 50-51 22.797130006470297 28.538226849283028 26.813651178653704 21.850991965592968 52-53 22.846547820344828 28.655555572705214 26.800279537886702 21.69761706906326 54-55 23.021581217793948 28.540622095571173 26.703097053788333 21.73469963284655 56-57 23.19003083085307 28.637895932720415 26.63229143934414 21.539781797082384 58-59 23.38098081836803 28.71031056103595 26.508379636807906 21.400328983788114 60-61 23.59070776904532 28.792270085619048 26.297285124665343 21.319737020670292 62-63 23.74842238279469 28.809321670244902 26.240828375500193 21.20142757146021 64-65 23.966942844068527 28.89238459042314 26.123674139051055 21.01699842645727 66-67 24.12770424374088 28.885982152890776 26.038987247103474 20.947326356264874 68-69 24.293814772884765 28.89084452585501 25.995055862467215 20.82028483879301 70-71 24.512918729708876 28.859256431288493 25.925299308357896 20.70252553064473 72-73 24.713472439681308 28.822302411012853 25.83823704172757 20.625988107578273 74-75 24.87689386127469 28.79503271708288 25.846552291221986 20.481521130420443 76-77 25.087923085254733 28.782680025307037 25.76775352524672 20.361643364191504 78-79 25.288558186729855 28.716938673501406 25.695918123260093 20.298585016508646 80-81 25.495636307241533 28.624003436782175 25.667881367970946 20.212478888005343 82-83 25.677719362777918 28.567924521639725 25.615290191496626 20.139065924085727 84-85 25.83704632360291 28.461215755717273 25.594612132488304 20.107125788191517 86-87 25.958395230735583 28.378418490066366 25.587697803152615 20.075488476045432 88-89 26.082125092785997 28.345564736529404 25.562177400268304 20.010132770416295 90-91 26.168894818745535 28.291010440760456 25.539475202944523 20.000619537549483 92-93 26.186640695046197 28.32624412330843 25.55983610064267 19.927279081002705 94-95 26.204998932550687 28.429147286154393 25.540831294222542 19.825022487072374 96-97 26.1886873099616 28.54978009570254 25.470810263715055 19.790722330620806 98-99 26.163363551809805 28.71027619455557 25.433781866386596 19.692578387248037 100 26.138665929945336 28.8850885791851 25.35038070123226 19.625864789637294 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 251003.0 1 145242.5 2 33654.0 3 25880.5 4 22498.5 5 19888.5 6 17934.5 7 16667.5 8 15893.5 9 15771.0 10 15902.0 11 17519.0 12 51801.0 13 54290.0 14 22685.0 15 22255.5 16 25101.5 17 30905.0 18 39534.5 19 50482.5 20 68376.5 21 99853.5 22 145326.0 23 197752.5 24 255187.5 25 321203.5 26 391361.5 27 471494.0 28 577584.0 29 684830.0 30 777293.5 31 882183.0 32 996092.5 33 1092159.5 34 1178798.0 35 1288712.5 36 1407137.5 37 1498747.5 38 1591299.0 39 1680115.5 40 1738669.5 41 1810584.0 42 1903043.5 43 1947091.0 44 1961329.5 45 2006307.5 46 2065039.5 47 2111591.5 48 2140692.0 49 2154744.0 50 2148548.5 51 2127181.0 52 2088383.0 53 2010972.5 54 1901583.5 55 1773358.0 56 1628173.0 57 1470869.5 58 1298113.0 59 1106782.5 60 911194.5 61 733008.5 62 575541.5 63 435339.5 64 319941.0 65 233132.5 66 168882.0 67 124757.5 68 92724.5 69 69815.5 70 54472.0 71 43157.5 72 35188.0 73 27769.5 74 21828.0 75 18623.5 76 14359.5 77 8769.0 78 5375.5 79 3854.0 80 2794.5 81 2113.0 82 1673.0 83 1342.0 84 1104.5 85 937.5 86 784.5 87 647.0 88 568.5 89 477.0 90 421.0 91 374.5 92 291.0 93 244.5 94 206.0 95 170.5 96 151.0 97 123.0 98 100.0 99 92.0 100 146.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004250998434186682 2 0.00272535462420125 3 0.001835973630425651 4 0.0030408855030846006 5 0.0032940036806503653 6 0.005745435893513321 7 0.006194460605770397 8 0.005034624572951927 9 0.005785310674910668 10-11 0.005812182810200184 12-13 0.005759305382695008 14-15 0.006557667853715794 16-17 0.00603149410788559 18-19 0.0069711519999448 20-21 0.7607804895537867 22-23 0.6072573801155595 24-25 0.5569891502626872 26-27 0.5148848484793848 28-29 0.5098987671185687 30-31 0.5018015859656858 32-33 0.4788077065885986 34-35 0.4721252133322476 36-37 0.4706862538296477 38-39 0.44827142562589584 40-41 0.4489865711618265 42-43 0.23389333187380074 44-45 0.14132662738908236 46-47 0.037842034389155704 48-49 0.013674449490068293 50-51 0.020208712580789993 52-53 0.0316536416849023 54-55 0.03975515705315449 56-57 0.036611984067354955 58-59 0.049385783603687526 60-61 0.04849986998220736 62-63 0.05602320102019801 64-65 0.05766326811593236 66-67 0.06094426915047489 68-69 0.06404930104102478 70-71 0.0593033352116667 72-73 0.06729649519568696 74-75 0.06927896530559416 76-77 0.07154575994372593 78-79 0.061506850305407014 80-81 0.06635770414670161 82-83 0.08192273838084844 84-85 0.0682335525585246 86-87 0.06495081783783437 88-89 0.0691133982784878 90-91 0.07073439482659731 92-93 0.07328117977758436 94-95 0.10732557466017975 96-97 0.06606297750159078 98-99 0.0610352876732297 100 0.0717832749459623 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5.7680567E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.47381914033596 #Duplication Level Percentage of deduplicated Percentage of total 1 74.31577307372478 30.82158931733911 2 11.762340019976408 9.756583253112746 3 4.430970270727331 5.513077788730524 4 2.3826646177836577 3.952728057201485 5 1.4638029398926 3.0354749193098884 6 1.0106194693696267 2.514854945540292 7 0.7545871475681534 2.190692761870452 8 0.5603519400012775 1.8591948011639499 9 0.4277269405841654 1.5965522797713212 >10 2.5354602649204603 19.155662281361863 >50 0.2036425613794041 5.896883060613239 >100 0.14800062097102332 11.791547487531643 >500 0.003923690523525558 0.9973552789241619 >1k 1.1971953077485593E-4 0.08266219789346423 >5k 0.0 0.0 >10k+ 1.6723046616896957E-5 0.8351415696357991 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 260317 0.45130797691361113 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 118501 0.20544354218986785 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 75018 0.1300576674289627 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.15646344114474464 6.934744590842874E-6 0.0 0.0 8.668430738553592E-6 2 0.1564721095754832 2.427160606795006E-5 0.0 0.0 1.7336861477107184E-5 3 0.15647904432007403 3.1206350658792936E-5 0.0 0.0 2.427160606795006E-5 4 0.15648077800622176 5.7211642874453716E-5 0.0 0.0 3.1206350658792936E-5 5 0.15648251169236946 6.58800736130073E-5 0.0 1.7336861477107185E-6 3.9874781397346525E-5 6 0.15658133180278896 7.108113205613947E-5 0.0 1.7336861477107185E-6 4.3342153692767964E-5 7 0.15658133180278896 7.974956279469305E-5 0.0 3.467372295421437E-6 4.507583984047868E-5 8 0.1565882665473798 8.668430738553593E-5 0.0 5.201058443132156E-6 5.0276898283610836E-5 9 0.15659346760582293 9.361905197637881E-5 0.0 6.934744590842874E-6 5.547795672674299E-5 10-11 0.15660300287963536 1.0835538423191991E-4 0.0 6.934744590842874E-6 5.8078485948309074E-5 12-13 0.1566116713103739 1.1529012882276279E-4 0.0 7.801587664698234E-6 6.414638746529659E-5 14-15 0.1566272744857033 1.2482540263517174E-4 0.0 1.126895996011967E-5 7.801587664698235E-5 16-17 0.15665761399328826 1.3782804874300212E-4 0.0 1.213580303397503E-5 9.015167968095736E-5 18-19 0.15668621981472547 1.4823016562926644E-4 0.0 1.213580303397503E-5 9.79532673456556E-5 20-21 0.1567399640853045 1.6990124247565042E-4 0.0 1.300264610783039E-5 1.0835538423191991E-4 22-23 0.156916800072371 1.9070547624817905E-4 0.0 1.3869489181685748E-5 1.2395855956131638E-4 24-25 0.1578954659027537 2.106428669468523E-4 0.0 1.6470018403251826E-5 1.3436067644758068E-4 26-27 0.16211785851550314 2.323139437932363E-4 0.0 1.9070547624817904E-5 1.4216226411227893E-4 28-29 0.17494973653778403 2.5225133449190953E-4 0.0 1.9070547624817904E-5 1.490970087031218E-4 30-31 0.20451515325776876 2.695881959690167E-4 0.0 2.427160606795006E-5 1.6123281173709684E-4 32-33 0.26143553685940707 2.851913712984132E-4 0.0 2.600529221566078E-5 1.7336861477107186E-4 34-35 0.3573621250983888 3.1293034966178466E-4 0.0 2.9472664511082216E-5 1.8203704550962544E-4 36-37 0.5013317570196562 3.320008972866026E-4 0.0 3.1206350658792936E-5 1.9070547624817905E-4 38-39 0.7013055194134967 3.4066932802515623E-4 0.0 3.294003680650365E-5 2.0977602387299695E-4 40-41 0.9580757068494143 3.5453881720684196E-4 0.0 3.294003680650365E-5 2.193112976854059E-4 42-43 1.2705596323281636 3.736093648316599E-4 0.0 3.380687988035901E-5 2.2971341457167022E-4 44-45 1.6396770163511047 3.8487832479177953E-4 0.0 3.9874781397346525E-5 2.4618343297492205E-4 46-47 2.0697776774628447 4.048157154904528E-4 0.0 4.160846754505725E-5 2.5571870678733097E-4 48-49 2.5615420874763593 4.1435098930286176E-4 0.0 4.420899676662332E-5 2.6265345137817383E-4 50-51 3.108843399545639 4.3775575229695646E-4 0.0 4.854321213590012E-5 2.7565609748600423E-4 52-53 3.7027184562870197 4.4902471225707614E-4 0.0 5.634479980059835E-5 2.851913712984132E-4 54-55 4.3444510522928805 4.7936421984201365E-4 0.0 5.8078485948309074E-5 2.9732717433238827E-4 56-57 5.031386914071077 4.949673951714102E-4 0.0 6.067901516987515E-5 3.068624481447972E-4 58-59 5.765866517921019 5.166384720177941E-4 0.0 6.327954439144123E-5 3.198650942526276E-4 60-61 6.536545488535159 5.426437642334549E-4 0.0 6.674691668686266E-5 3.3373458343431337E-4 62-63 7.337570901478829 5.547795672674299E-4 0.0 6.761375976071802E-5 3.528051310591312E-4 64-65 8.168411381947754 5.660485272275496E-4 0.0 6.761375976071802E-5 3.6147356179768483E-4 66-67 9.027005577112305 5.816517025569461E-4 0.0 6.934744590842874E-5 3.710088356100938E-4 68-69 9.913120306185617 5.93787505590921E-4 0.0 7.108113205613945E-5 3.7620989405322595E-4 70-71 10.822487233872025 6.171922685850159E-4 0.0 7.714903357312699E-5 3.900793832349117E-4 72-73 11.751371133366288 6.362628162098337E-4 0.0 8.581746431168057E-5 3.9614728475189916E-4 74-75 12.694158328922114 6.53599677686941E-4 0.0 8.9284836607102E-5 3.996146570473206E-4 76-77 13.653674728960276 6.648686376470606E-4 0.0 9.188536582866809E-5 4.039488724165974E-4 78-79 14.625851372092095 6.848060283457338E-4 0.0 9.188536582866809E-5 4.1175046008129566E-4 80-81 15.61277648328249 7.012760467489857E-4 0.0 9.621958119794488E-5 4.256199492629814E-4 82-83 16.61140137544071 7.186129082260928E-4 0.0 1.0055379656722169E-4 4.308210077061136E-4 84-85 17.616102837546656 7.402839850724768E-4 0.0 1.0315432578878776E-4 4.403562815185225E-4 86-87 18.631102395370004 7.654224342142822E-4 0.0 1.0488801193649848E-4 4.4729102610936537E-4 88 19.399056878203016 7.766913941744019E-4 0.0 1.0575485501035384E-4 4.576931429956297E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 36880 0.0 51.438484 8 GTATCAA 42185 0.0 44.959007 9 GTGGTAT 42595 0.0 44.52621 6 TGGTATC 42980 0.0 44.018234 7 AGTGGTA 44095 0.0 43.054073 5 CAACGCA 28655 0.0 34.999973 12-13 TCAACGC 27305 0.0 34.893044 12-13 ATCAACG 32215 0.0 31.299511 10-11 AACGCAG 31795 0.0 30.103918 14-15 CAGTGGT 64445 0.0 29.880737 4 GCAGTGG 65950 0.0 29.36237 3 AAGCAGT 69965 0.0 27.838055 1 ACGCAGA 36105 0.0 27.806673 14-15 AGAGTAC 40595 0.0 24.340965 18-19 CGCAGAG 39530 0.0 24.17748 16-17 GAGTACT 34970 0.0 23.484304 20-21 AGCAGTG 83685 0.0 23.319017 2 TATCAAC 43425 0.0 22.16692 10-11 AGTACTT 52255 0.0 16.574928 20-21 GTACTTT 51205 0.0 16.069244 22-23 >>END_MODULE