FastQCFastQC Report
Thu 26 May 2016
SRR1660668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57680567
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11767672.040144646983099No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1655200.28695973116907814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1287890.22327970527751573No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1650950.080.054428
GTGGTAT1699200.078.071276
TGGTATC1701300.077.723887
AGTGGTA1712300.077.5207065
GTATCAA1707050.077.258389
GCAGTGG1903150.070.232033
AAGCAGT1918900.069.9676061
CAGTGGT1910750.069.7220464
AGCAGTG2095700.064.165232
CAACGCA1566200.044.00683212-13
ATCAACG1608550.042.91979210-11
TCAACGC1557600.042.2149412-13
ACGCAGA1648250.041.93746614-15
AACGCAG1602850.040.97492214-15
AGAGTAC1690000.040.5712218-19
CGCAGAG1679550.039.05770516-17
GAGTACT1640850.038.77898420-21
TATCAAC1719200.038.38488410-11
AGTACTT1806750.037.28214320-21
GTACTTT1792800.035.3705922-23
Produced by FastQC (version 0.11.5)