##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660668_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57680567 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.281461605604534 34.0 31.0 34.0 30.0 34.0 2 32.49053546925085 34.0 31.0 34.0 31.0 34.0 3 32.499655542567744 34.0 31.0 34.0 31.0 34.0 4 36.03080092815315 37.0 35.0 37.0 35.0 37.0 5 35.91653254726154 37.0 35.0 37.0 35.0 37.0 6 35.900614205127354 37.0 35.0 37.0 35.0 37.0 7 35.87420144465639 37.0 35.0 37.0 35.0 37.0 8 35.873873587269 37.0 35.0 37.0 35.0 37.0 9 37.55130352654127 39.0 37.0 39.0 35.0 39.0 10-11 37.51798009371163 39.0 37.0 39.0 34.5 39.0 12-13 37.53360415995911 39.0 37.0 39.0 35.0 39.0 14-15 38.928014837302136 40.0 38.0 41.0 35.0 41.0 16-17 38.90386720713061 40.0 38.0 41.0 35.0 41.0 18-19 38.89879529096862 40.0 38.0 41.0 35.0 41.0 20-21 38.898584734785985 40.0 38.0 41.0 35.0 41.0 22-23 38.82855848140328 40.0 38.0 41.0 34.5 41.0 24-25 38.812462835186764 40.0 38.0 41.0 34.0 41.0 26-27 38.73620474292494 40.0 38.0 41.0 34.0 41.0 28-29 38.63647976969436 40.0 38.0 41.0 34.0 41.0 30-31 38.59487695396614 40.0 38.0 41.0 34.0 41.0 32-33 38.517646948581486 40.0 38.0 41.0 34.0 41.0 34-35 38.458649895033105 40.0 38.0 41.0 34.0 41.0 36-37 38.37205451707852 40.0 38.0 41.0 34.0 41.0 38-39 38.19003842316599 40.0 38.0 41.0 33.5 41.0 40-41 38.11233298902904 40.0 37.0 41.0 33.0 41.0 42-43 38.02764427575755 40.0 37.0 41.0 33.0 41.0 44-45 37.95214016360137 40.0 37.0 41.0 33.0 41.0 46-47 37.854281122097845 40.0 37.0 41.0 33.0 41.0 48-49 37.74425526191516 40.0 37.0 41.0 33.0 41.0 50-51 37.57680305049706 40.0 36.0 41.0 32.0 41.0 52-53 37.426884369912656 40.0 36.0 41.0 32.0 41.0 54-55 37.16625654009261 39.5 36.0 41.0 31.0 41.0 56-57 36.8870880378135 39.0 35.5 41.0 31.0 41.0 58-59 36.64693570019865 39.0 35.0 41.0 30.5 41.0 60-61 36.43356013646676 39.0 35.0 40.5 30.0 41.0 62-63 36.213641381160485 38.0 35.0 40.0 30.0 41.0 64-65 35.948150232295745 37.5 35.0 40.0 29.5 41.0 66-67 35.66336110218889 37.0 34.5 40.0 29.0 41.0 68-69 35.35109829970985 36.5 34.0 39.5 29.0 41.0 70-71 35.04075558931312 36.0 34.0 39.0 29.0 41.0 72-73 34.70792677887511 35.5 34.0 39.0 29.0 40.0 74-75 34.34324602773062 35.0 34.0 37.5 28.5 40.0 76-77 34.035416624805364 35.0 34.0 37.0 28.5 39.0 78-79 33.872527293291 35.0 34.0 37.0 29.0 39.0 80-81 33.622224084586406 35.0 34.0 36.0 28.5 39.0 82-83 33.37461642844114 35.0 33.0 36.0 28.0 37.0 84-85 33.154387863420276 35.0 33.0 35.5 28.0 37.0 86-87 32.965140945996595 35.0 33.0 35.0 27.0 37.0 88-89 32.81741049806254 35.0 33.0 35.0 27.0 36.0 90-91 32.67570285846878 35.0 33.0 35.0 27.0 36.0 92-93 32.56472069700702 35.0 33.0 35.0 27.0 36.0 94-95 32.464625807163095 35.0 33.0 35.0 27.0 35.0 96-97 32.36909219356322 35.0 33.0 35.0 26.5 35.0 98-99 32.313278317808496 35.0 33.0 35.0 26.0 35.0 100 32.28283317672658 35.0 33.0 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04463345569509869 1101 2 -0.0023211585093250164 1101 3 -0.023872791435366025 1101 4 0.024133178969300673 1101 5 -0.004932270872473055 1101 6 0.05227707329301268 1101 7 0.07419175570716163 1101 8 0.043957729857098116 1101 9 -0.01106016755392858 1101 10-11 0.037941958853444646 1101 12-13 0.013651387983991015 1101 14-15 0.03682563107094694 1101 16-17 0.046198303883699054 1101 18-19 0.020914982393762216 1101 20-21 0.03216127583743145 1101 22-23 0.11898762788031547 1101 24-25 0.09849818579679237 1101 26-27 0.07408713647836862 1101 28-29 0.13636482151980545 1101 30-31 0.12572833360956537 1101 32-33 0.100265479057974 1101 34-35 0.16393001098824556 1101 36-37 0.15981263636314935 1101 38-39 0.2298050698622589 1101 40-41 0.27923071133239574 1101 42-43 0.28630364084194326 1101 44-45 0.3218466098962125 1101 46-47 0.20179881680146394 1101 48-49 0.20622777825938243 1101 50-51 0.2604113396579777 1101 52-53 0.1933529822183644 1101 54-55 0.29641820188714263 1101 56-57 0.3353753230255947 1101 58-59 0.2612236123162077 1101 60-61 0.2708912989643082 1101 62-63 0.2335618692232586 1101 64-65 0.2443278899596848 1101 66-67 0.330908786667365 1101 68-69 0.31166941207124665 1101 70-71 0.34678031384178354 1101 72-73 0.3724711190792007 1101 74-75 0.3622033467445931 1101 76-77 0.8425689185040639 1101 78-79 0.42929118021154267 1101 80-81 0.3237319816496367 1101 82-83 0.3859156543495743 1101 84-85 0.33560922573871466 1101 86-87 0.31207843155045367 1101 88-89 0.27967015336343337 1101 90-91 0.3929102205439676 1101 92-93 0.37782833690300777 1101 94-95 0.33834736993703274 1101 96-97 0.2833525888094073 1101 98-99 0.3084165271774353 1101 100 0.29021819430861484 1102 1 0.013169719289322757 1102 2 -0.039830124286496016 1102 3 -0.041644024163311144 1102 4 0.003211513232791674 1102 5 -0.01974760441903811 1102 6 0.038755250790991624 1102 7 0.059596007240749316 1102 8 0.024476030304114715 1102 9 -0.009536331630052075 1102 10-11 0.025073420981009065 1102 12-13 0.002352003640886835 1102 14-15 -0.008548134306764155 1102 16-17 0.015017238258977272 1102 18-19 0.009980791472663952 1102 20-21 0.003761602473112191 1102 22-23 0.08372662920863405 1102 24-25 0.06360746270009798 1102 26-27 0.06769102745101918 1102 28-29 0.08595504297870349 1102 30-31 0.11617098386285818 1102 32-33 0.06675770053216468 1102 34-35 0.09062105597136139 1102 36-37 0.11337809838043 1102 38-39 0.19854590099037495 1102 40-41 0.26511838451349234 1102 42-43 0.2805010640649286 1102 44-45 0.3016216428139842 1102 46-47 0.2575558502974644 1102 48-49 0.2034730619925469 1102 50-51 0.20014297288356175 1102 52-53 0.1897577500066916 1102 54-55 0.3024149634273172 1102 56-57 0.36446851244693335 1102 58-59 0.24663029830590943 1102 60-61 0.27851175802836536 1102 62-63 0.2590747051585893 1102 64-65 0.24934797386541874 1102 66-67 0.35443290790157533 1102 68-69 0.31142204197417556 1102 70-71 0.3495289969360016 1102 72-73 0.3593538598139929 1102 74-75 0.332979909596979 1102 76-77 0.8377073805752531 1102 78-79 0.4093629285990943 1102 80-81 0.3035037877805067 1102 82-83 0.38074619245028885 1102 84-85 0.35462027017280917 1102 86-87 0.3163679333555578 1102 88-89 0.2682294033992818 1102 90-91 0.4205610190885096 1102 92-93 0.38288114025160525 1102 94-95 0.3226124962380723 1102 96-97 0.31165846628908866 1102 98-99 0.3484510724101355 1102 100 0.30137492476517735 1103 1 -0.05693585054696371 1103 2 -0.0968618451216372 1103 3 -0.12282445132797193 1103 4 -0.06637150413685333 1103 5 -0.052277427913004715 1103 6 -0.025910699323787867 1103 7 -0.020397590425474732 1103 8 -0.06173479768704482 1103 9 -0.12859871848720417 1103 10-11 -0.11088691084218993 1103 12-13 -0.0979167933265046 1103 14-15 -0.11249370523010782 1103 16-17 -0.13430066022697673 1103 18-19 -0.14922946051102315 1103 20-21 -0.12463636347943208 1103 22-23 -0.05391527806535379 1103 24-25 -0.07068949791125334 1103 26-27 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0.27786436311783547 1103 92-93 0.2869412860846694 1103 94-95 0.20699914923792306 1103 96-97 0.14929729014586002 1103 98-99 0.1940960570450443 1103 100 0.13482084560501306 1104 1 -0.07371776875265112 1104 2 -0.11902425496835889 1104 3 -0.1464227873056032 1104 4 -0.05919760758730064 1104 5 -0.07166441442015525 1104 6 -0.025758452463634285 1104 7 0.02004164690571031 1104 8 -0.03420725569495886 1104 9 -0.09434488277748443 1104 10-11 -0.06838506074672779 1104 12-13 -0.059636423897764246 1104 14-15 -0.10462734915995497 1104 16-17 -0.09342658188007391 1104 18-19 -0.12314850866592053 1104 20-21 -0.09979218544290802 1104 22-23 -0.02002988530782801 1104 24-25 -0.03765347607467362 1104 26-27 -0.060298789883376 1104 28-29 -0.011417727345104822 1104 30-31 0.014682706709017168 1104 32-33 -0.020722721241860143 1104 34-35 -0.011632072684051309 1104 36-37 -0.005358984383612153 1104 38-39 0.08301476351129367 1104 40-41 0.162829609175283 1104 42-43 0.12977291232898125 1104 44-45 0.1620525023311714 1104 46-47 0.10114719600971966 1104 48-49 0.06563641966828015 1104 50-51 0.10579815697340678 1104 52-53 0.04709121012790973 1104 54-55 0.17589959750996087 1104 56-57 0.23264397816806337 1104 58-59 0.15330539763171913 1104 60-61 0.1472823126285263 1104 62-63 0.11155723743062396 1104 64-65 0.13126617382682326 1104 66-67 0.24991202129539403 1104 68-69 0.16511799063511745 1104 70-71 0.2058769553817399 1104 72-73 0.2502213545351353 1104 74-75 0.2455872503727008 1104 76-77 0.7312387622656615 1104 78-79 0.3385421797206263 1104 80-81 0.24220152366269332 1104 82-83 0.3219802303635717 1104 84-85 0.26123971682665115 1104 86-87 0.2523886272039775 1104 88-89 0.19424169091995935 1104 90-91 0.3135935351548369 1104 92-93 0.31583547339104 1104 94-95 0.23378250747175855 1104 96-97 0.1926258157926526 1104 98-99 0.2297583110516186 1104 100 0.2007868097211727 1105 1 -0.12820776996726124 1105 2 -0.1434357457768627 1105 3 -0.16256378743614874 1105 4 -0.10466355723611542 1105 5 -0.12352067653393561 1105 6 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1105 66-67 0.14077058604487291 1105 68-69 0.06298027233364678 1105 70-71 0.13505015326185799 1105 72-73 0.15873345400135008 1105 74-75 0.12312851522820267 1105 76-77 0.6624448213070693 1105 78-79 0.21756211670424364 1105 80-81 0.12110995683746495 1105 82-83 0.18797103020218486 1105 84-85 0.12679111143138044 1105 86-87 0.1329059096747116 1105 88-89 0.10669998179267992 1105 90-91 0.2262842569244441 1105 92-93 0.19413836017633912 1105 94-95 0.15090938621248284 1105 96-97 0.0861592829168103 1105 98-99 0.14174023184840223 1105 100 0.1360184828730624 1106 1 -0.17726880409594514 1106 2 -0.2110604671358658 1106 3 -0.22510275025893378 1106 4 -0.1440125177046241 1106 5 -0.17665694380922758 1106 6 -0.11470544757487744 1106 7 -0.0644580190612345 1106 8 -0.09833391672206915 1106 9 -0.19233599139191426 1106 10-11 -0.18618579501075772 1106 12-13 -0.15125176515127947 1106 14-15 -0.21168539765405114 1106 16-17 -0.21520618853170959 1106 18-19 -0.2595909672840975 1106 20-21 -0.2308600878308269 1106 22-23 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1106 86-87 0.13543352495415917 1106 88-89 0.09108980594182725 1106 90-91 0.16765226953805978 1106 92-93 0.1844292110967345 1106 94-95 0.18595795198860543 1106 96-97 0.09399576782801233 1106 98-99 0.08406839467694738 1106 100 0.05969220982891699 1107 1 -0.2157006520183984 1107 2 -0.21704976271404774 1107 3 -0.23791274519854966 1107 4 -0.15289093684629051 1107 5 -0.23050744077312402 1107 6 -0.1445584351857434 1107 7 -0.1293637940545196 1107 8 -0.14095825255430583 1107 9 -0.22859822924504414 1107 10-11 -0.21064766536358803 1107 12-13 -0.19871114673843948 1107 14-15 -0.2669076136111599 1107 16-17 -0.23022011815074706 1107 18-19 -0.2717576830393327 1107 20-21 -0.26029160614439917 1107 22-23 -0.1778216119927052 1107 24-25 -0.17418078643618173 1107 26-27 -0.20821694327274543 1107 28-29 -0.18828153945099046 1107 30-31 -0.21524746799698136 1107 32-33 -0.22770780278882086 1107 34-35 -0.21550968738662846 1107 36-37 -0.22007167109774173 1107 38-39 -0.12171594180403389 1107 40-41 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0.9610476451999901 2316 22-23 0.6721753259614971 2316 24-25 0.7969083978348195 2316 26-27 1.1998425799813077 2316 28-29 1.520759588198409 2316 30-31 1.751592763202062 2316 32-33 1.0257898554752032 2316 34-35 1.0889637428045589 2316 36-37 1.2898201949421306 2316 38-39 0.6704950885597967 2316 40-41 1.290056386631754 2316 42-43 1.2442395686243586 2316 44-45 0.5430606071910375 2316 46-47 1.175898666292916 2316 48-49 1.3568241766627978 2316 50-51 0.8439344662575436 2316 52-53 1.2124790049471486 2316 54-55 1.8803006096260688 2316 56-57 1.5227431307724757 2316 58-59 -0.2964590367668123 2316 60-61 0.9150160785057508 2316 62-63 0.3219064906975788 2316 64-65 0.7427316374235815 2316 66-67 -1.066652991455193 2316 68-69 0.7644780208948916 2316 70-71 0.2374266552243398 2316 72-73 -0.4261996650239084 2316 74-75 -0.4574010544700826 2316 76-77 -1.0425858500310312 2316 78-79 0.3852160452842739 2316 80-81 -0.12774475914414296 2316 82-83 -0.3348134998852217 2316 84-85 0.0168303009134263 2316 86-87 -0.13228603450887277 2316 88-89 0.6673270436049066 2316 90-91 0.5835331071255041 2316 92-93 0.21676637313114355 2316 94-95 0.2868926749986329 2316 96-97 -0.004658278305470276 2316 98-99 -0.2338083306019314 2316 100 0.5123533934174986 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 20.0 8 138.0 9 256.0 10 223.0 11 227.0 12 520.0 13 2086.0 14 6603.0 15 15567.0 16 23275.0 17 28899.0 18 39027.0 19 55773.0 20 81766.0 21 118138.0 22 172554.0 23 253762.0 24 370381.0 25 506512.0 26 616855.0 27 679698.0 28 755570.0 29 890731.0 30 1093798.0 31 1308864.0 32 1611715.0 33 2041948.0 34 2719040.0 35 3932937.0 36 6325868.0 37 1.2401836E7 38 1.6679981E7 39 4934710.0 40 11288.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.389348945228924 31.146910725906597 26.680240287569195 23.783500041295287 2 21.512203228838615 28.780146598453264 28.89772868686386 20.80992148584426 3 21.18745619703796 27.559473529448354 27.533307773635784 23.719762499877906 4 19.637345039570686 27.7855082261024 28.058339692026795 24.51880704230011 5 21.370181527981202 29.49564006703551 27.61341559017008 21.520762814813207 6 23.725736806433783 26.90862353448526 27.90055585320013 21.465083805880823 7 21.53796789133505 27.528777586392312 29.56059880618025 21.37265571609239 8 22.996821442479927 26.97605937195451 28.079032232814217 21.94808695275135 9 23.147251655830637 26.652825725516884 28.430641120431428 21.769281498221055 10-11 21.2570249179416 28.455719757401138 28.698533771347982 21.58872155330928 12-13 21.36235848721806 27.24375351580715 28.464029315107116 22.929858681867675 14-15 21.28011588374296 29.748427230266305 27.257409761592672 21.714047124398068 16-17 22.610427009151973 27.190046519480294 27.25047154962953 22.9490549217382 18-19 21.513239831939234 28.29913804937351 27.28554796081578 22.902074157871475 20-21 22.687954296005874 28.26438113871482 27.275415834382727 21.77224873089657 22-23 22.7435270672488 27.161386269292663 28.396242101690483 21.698844561768052 24-25 21.60537931622506 28.222596940203953 27.096350130622998 23.075673612947988 26-27 21.633240555949975 26.963987166890966 29.59924947022486 21.8035228069342 28-29 21.710744104793697 27.09283344635426 29.442145182430824 21.754277266421223 30-31 21.806502606926827 26.96054729793082 29.482309184179005 21.750640910963345 32-33 21.764283143570207 27.0865375418742 29.35493425631648 21.794245058239117 34-35 21.792669070834446 27.06320626590129 29.30845565547836 21.835669007785903 36-37 21.8232801577601 27.12637906970899 29.262726007009977 21.787614765520942 38-39 21.87131895472941 27.144657499204683 29.257151994055985 21.72687155200993 40-41 21.90726408341287 27.307564320078576 29.191591015026745 21.59358058148181 42-43 21.943897079949505 27.172596887503897 29.178398537870493 21.705107494676103 44-45 21.98392559275597 27.236486656621935 29.116053964931204 21.663533785690895 46-47 22.121467399605123 27.348348658800887 28.988001327760838 21.542182613833152 48-49 22.113049911354338 27.446471568485396 28.85750580596149 21.58297271419877 50-51 22.234137158222836 27.441965521700677 28.686359191448123 21.63753812862836 52-53 22.31626156987173 27.577881843928758 28.45459442069942 21.651262165500093 54-55 22.42204866185053 27.715775937877446 28.085483715424214 21.77669168484781 56-57 22.611784389780006 27.968708546097876 27.661895258401113 21.75761180572101 58-59 22.65384218165762 28.135094684249772 27.37105629104416 21.84000684304845 60-61 22.715823683917446 28.208397822016373 27.15088470236186 21.924893791704317 62-63 22.599215819221442 28.260008407404374 27.249145157790274 21.891630615583914 64-65 22.679263392478084 28.403705747527624 26.974436362900956 21.94259449709334 66-67 22.566043294811642 28.427586683126073 26.92525134990884 22.081118672153448 68-69 22.490899599246983 28.39135022025158 26.94504159865564 22.172708581845797 70-71 22.42620261956292 28.41847884571865 26.879936008951006 22.275382525767427 72-73 22.353639794493304 28.427191436821143 26.75637291661401 22.462795852071547 74-75 22.288618366508583 28.45330225805615 26.66398883357993 22.594090541855337 76-77 22.28400494637829 28.5240968806068 26.459521067359137 22.732377105655775 78-79 22.25664560580219 28.56696331359096 26.26159624122878 22.91479483937807 80-81 22.18166798717454 28.625098821364915 26.11327222457225 23.0799609668883 82-83 22.146873068991813 28.688523950669314 25.936912321407558 23.227690658931316 84-85 22.0455645418211 28.68925807756141 25.82483731748198 23.440340063135512 86-87 21.979145763050624 28.67180047990462 25.75528924894734 23.59376450809742 88-89 21.91527198364689 28.662950898502913 25.687049212455904 23.73472790539429 90-91 21.870678049846198 28.586682430287752 25.621962290487964 23.920677229378086 92-93 21.803113899989242 28.527992730788487 25.63582564131628 24.03306772790599 94-95 21.763259266990527 28.494466154440033 25.630362019510127 24.111912559059313 96-97 21.699302736614474 28.42742840999871 25.619194050052396 24.25407480333442 98-99 21.635286374165823 28.38740304162654 25.64434854798488 24.332962036222757 100 21.580381951349196 28.412658928092245 25.599450912558456 24.407508208000102 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 227099.0 1 132807.0 2 25970.5 3 10892.0 4 7461.0 5 6143.5 6 5563.5 7 5438.5 8 5307.5 9 5188.5 10 5686.5 11 11774.5 12 148945.0 13 166704.0 14 46121.0 15 40462.0 16 45083.0 17 53786.0 18 64619.0 19 77003.5 20 95168.0 21 126426.0 22 170476.5 23 220025.5 24 274213.0 25 336784.5 26 404024.0 27 480889.5 28 583710.5 29 691045.0 30 781621.5 31 882566.0 32 992934.5 33 1084111.0 34 1168324.0 35 1278262.5 36 1397455.5 37 1487155.5 38 1579687.5 39 1673863.0 40 1739174.5 41 1818219.5 42 1915056.5 43 1960784.0 44 1973574.5 45 2013313.0 46 2056198.0 47 2080220.5 48 2101059.0 49 2127225.0 50 2144744.5 51 2141859.5 52 2106620.0 53 2025500.5 54 1908959.0 55 1771949.5 56 1620422.0 57 1455953.5 58 1276521.0 59 1080273.0 60 881985.5 61 705939.0 62 551653.5 63 413443.5 64 301030.5 65 216594.5 66 154130.0 67 112927.0 68 83160.0 69 61812.5 70 47431.5 71 36825.5 72 29638.0 73 22569.0 74 17331.0 75 14711.0 76 10881.0 77 5835.5 78 2949.5 79 1863.0 80 1118.0 81 698.0 82 459.0 83 277.0 84 170.0 85 100.0 86 56.0 87 33.5 88 23.0 89 17.0 90 9.0 91 6.5 92 3.0 93 1.5 94 2.0 95 2.0 96 1.5 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039273192304090904 2 2.253791992023934E-5 3 0.06994383394324123 4 0.0017388872061538509 5 1.6123281173709684E-4 6 0.0063244870668487 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.7336861477107187E-6 20-21 3.1466403580949543E-4 22-23 4.854321213590013E-5 24-25 7.76691394174402E-4 26-27 0.006890535594076251 28-29 0.0106509008484608 30-31 0.01347854295537698 32-33 0.01563611536620297 34-35 0.01857644707272035 36-37 0.02430714663397813 38-39 0.020500838696679246 40-41 0.022064623601914315 42-43 0.02046443128757732 44-45 0.027494528616544285 46-47 0.026111913913744986 48-49 0.03729592325262683 50-51 0.003110232948993029 52-53 0.02526934244595758 54-55 0.027686967778940176 56-57 0.03228990450111213 58-59 0.017966189548726176 60-61 0.00599421985570981 62-63 0.002856247928353409 64-65 0.0015993254712631379 66-67 8.122319602024717E-4 68-69 4.8109790598972437E-4 70-71 0.002167107684638398 72-73 5.287742750517692E-4 74-75 0.0014658316378894126 76-77 0.004873391761214829 78-79 0.006113844199901849 80-81 0.006751840702259393 82-83 0.00316224353342435 84-85 0.005273873261336006 86-87 0.004589934076064128 88-89 0.002619599769190896 90-91 1.3956173489071286E-4 92-93 0.0010116058671892042 94-95 0.004025619234984288 96-97 0.00546371189451033 98-99 0.0029316632757788253 100 0.0014996385177697715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5.7680567E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.92757155384866 #Duplication Level Percentage of deduplicated Percentage of total 1 71.91321799174052 27.27493721049255 2 12.559694523807677 9.527174254923937 3 4.931296788915686 5.6109633544459125 4 2.588368424694458 3.926821145412863 5 1.700721636728842 3.2252120785105878 6 1.1281832890304655 2.567355145233558 7 0.8309521711559331 2.206119855053978 8 0.6102867918340745 1.851735677252448 9 0.48727533000722584 1.6633052950746847 >10 2.8542624811331123 19.74767463748664 >50 0.22393351097604372 5.965811270788299 >100 0.1664275495481323 12.235267349423692 >500 0.004929425294705534 1.1405421760155714 >1k 3.861024734458669E-4 0.2362059098299877 >5k 3.656177317361867E-5 0.09464368526112178 >10k+ 2.742088657976506E-5 2.726230954794214 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1176767 2.040144646983099 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 165520 0.28695973116907814 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 128789 0.22327970527751573 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.15970370055481597 6.934744590842874E-6 0.0 1.7336861477107185E-6 0.0 2 0.1597089016132591 1.3869489181685748E-5 0.0 1.7336861477107185E-6 1.7336861477107185E-6 3 0.15971063529940682 2.0804233772528624E-5 0.0 3.467372295421437E-6 6.934744590842874E-6 4 0.15971583635784994 3.294003680650365E-5 0.0 5.201058443132156E-6 1.213580303397503E-5 5 0.15971583635784994 3.9874781397346525E-5 0.0 5.201058443132156E-6 1.7336861477107184E-5 6 0.15982505858515572 3.9874781397346525E-5 0.0 5.201058443132156E-6 2.427160606795006E-5 7 0.15982679227130345 4.6809525988189404E-5 0.0 8.668430738553592E-6 2.9472664511082216E-5 8 0.15983199332974657 5.547795672674299E-5 0.0 8.668430738553592E-6 3.1206350658792936E-5 9 0.15983372701589427 5.7211642874453716E-5 0.0 8.668430738553592E-6 3.294003680650365E-5 10-11 0.159841528603559 6.848060283457338E-5 0.0 1.0402116886264312E-5 3.467372295421437E-5 12-13 0.15984933019122366 7.974956279469306E-5 0.0 1.126895996011967E-5 3.987478139734653E-5 14-15 0.15989440603106414 9.361905197637881E-5 0.0 1.3869489181685748E-5 4.941005520975548E-5 16-17 0.15992387869557523 1.0315432578878776E-4 0.0 1.5603175329396468E-5 5.894532902216443E-5 18-19 0.159955085046234 1.1875750111818422E-4 0.0 1.5603175329396468E-5 7.628219049927162E-5 20-21 0.16001576406140391 1.3089330415215925E-4 0.0 1.8203704550962546E-5 8.581746431168057E-5 22-23 0.1601847984608057 1.5256438099854323E-4 0.0 2.253791992023934E-5 9.101852275481272E-5 24-25 0.16117733378037008 1.6903439940179507E-4 0.0 2.600529221566078E-5 9.535273812408952E-5 26-27 0.16545780487906786 1.9850706391287727E-4 0.0 3.554056602806973E-5 1.0142063964107704E-4 28-29 0.17845611677152895 2.1844445461155054E-4 0.0 3.640740910192509E-5 1.0488801193649848E-4 30-31 0.20844368606848124 2.3144710071938094E-4 0.0 3.727425217578045E-5 1.1355644267505207E-4 32-33 0.26584170020381387 2.496508052703435E-4 0.0 4.160846754505725E-5 1.2135803033975031E-4 34-35 0.3628015653868312 2.635202944520292E-4 0.0 4.2475310618912606E-5 1.3262699029986997E-4 36-37 0.5083003084903794 2.8172399900299177E-4 0.0 4.594268291433404E-5 1.430291071861343E-4 38-39 0.7098855321585171 2.8692505744612397E-4 0.0 4.6809525988189404E-5 1.5429806714625395E-4 40-41 0.968769083008494 2.9472664511082217E-4 0.0 4.854321213590012E-5 1.6470018403251827E-4 42-43 1.2837581849706852 3.0772929121865257E-4 0.0 4.854321213590012E-5 1.7336861477107186E-4 44-45 1.6546543309811779 3.2766668191732583E-4 0.0 4.941005520975548E-5 1.8203704550962544E-4 46-47 2.0859581702794285 3.45003543394433E-4 0.0 5.201058443132156E-5 1.9243916239588976E-4 48-49 2.5786093607574974 3.528051310591312E-4 0.0 5.287742750517692E-5 2.089091807991416E-4 50-51 3.127063574115005 3.6580777716696165E-4 0.0 5.3744270579032276E-5 2.1411023924227375E-4 52-53 3.7225076168200637 3.78810423274792E-4 0.0 5.3744270579032276E-5 2.210449838331166E-4 54-55 4.3660406458903225 4.0741624471201884E-4 0.0 5.634479980059835E-5 2.3664815916251309E-4 56-57 5.0549598446215 4.195520477459939E-4 0.0 6.0679015169875155E-5 2.4444974682721134E-4 58-59 5.79146266020582 4.386225953708118E-4 0.0 6.241270131758587E-5 2.539850206396203E-4 60-61 6.564585261445159 4.4902471225707614E-4 0.0 6.241270131758587E-5 2.6698766674745065E-4 62-63 7.367314887872028 4.663615737341833E-4 0.0 6.934744590842874E-5 2.8345768515070254E-4 64-65 8.199199914244948 4.8456527828514586E-4 0.0 7.194797512999483E-5 2.9125927281540073E-4 66-67 9.058665286698725 4.99301610540687E-4 0.0 7.714903357312697E-5 3.007945466278097E-4 68-69 9.946093283028926 5.045026689838191E-4 0.0 7.974956279469305E-5 3.0772929121865257E-4 70-71 10.856461414465638 5.131710997223727E-4 0.0 8.148324894240378E-5 3.1813140810491687E-4 72-73 11.786031853674393 5.33108490421046E-4 0.0 8.581746431168057E-5 3.2766668191732583E-4 74-75 12.729387698286668 5.513121949720085E-4 0.0 9.015167968095736E-5 3.3980248495130085E-4 76-77 13.689806481964714 5.703827425968264E-4 0.0 9.535273812408952E-5 3.45003543394433E-4 78-79 14.664265522909995 5.842522317785121E-4 0.0 9.708642427180024E-5 3.554056602806973E-4 80-81 15.654366747122996 6.041896224771854E-4 0.0 9.708642427180024E-5 3.675414633146723E-4 82-83 16.656839555686062 6.154585824373051E-4 0.0 9.708642427180024E-5 3.814109524963581E-4 84-85 17.665708799291103 6.327954439144123E-4 0.0 1.0055379656722167E-4 3.952804416780438E-4 86-87 18.68507724620668 6.483986192438087E-4 0.0 1.0402116886264312E-4 4.0741624471201884E-4 88 19.456008468155314 6.640017945732052E-4 0.0 1.0748854115806455E-4 4.1261730315515104E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 165095 0.0 80.05442 8 GTGGTAT 169920 0.0 78.07127 6 TGGTATC 170130 0.0 77.72388 7 AGTGGTA 171230 0.0 77.520706 5 GTATCAA 170705 0.0 77.25838 9 GCAGTGG 190315 0.0 70.23203 3 AAGCAGT 191890 0.0 69.967606 1 CAGTGGT 191075 0.0 69.722046 4 AGCAGTG 209570 0.0 64.16523 2 CAACGCA 156620 0.0 44.006832 12-13 ATCAACG 160855 0.0 42.919792 10-11 TCAACGC 155760 0.0 42.21494 12-13 ACGCAGA 164825 0.0 41.937466 14-15 AACGCAG 160285 0.0 40.974922 14-15 AGAGTAC 169000 0.0 40.57122 18-19 CGCAGAG 167955 0.0 39.057705 16-17 GAGTACT 164085 0.0 38.778984 20-21 TATCAAC 171920 0.0 38.384884 10-11 AGTACTT 180675 0.0 37.282143 20-21 GTACTTT 179280 0.0 35.37059 22-23 >>END_MODULE