FastQCFastQC Report
Thu 26 May 2016
SRR1660667_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660667_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28959818
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8841053.0528679427474303No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4498811.5534662545185884No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1866230.6444204863442167Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1617220.5584358299489313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1262750.080.6476068
GTGGTAT1275300.080.045346
AGTGGTA1282250.079.61865
AAGCAGT1302850.079.115441
GTATCAA1289650.078.729739
TGGTATC1310850.077.720387
GCAGTGG1339200.076.809353
CAGTGGT1356050.075.497824
AGCAGTG1369550.075.468322
CAACGCA1234400.043.029912-13
ATCAACG1249200.042.61242710-11
ACGCAGA1263900.042.01107814-15
TCAACGC1229000.041.12140312-13
AGAGTAC1283700.041.02643218-19
GAGTACT1165100.040.44507220-21
AACGCAG1251850.040.26237514-15
AGTACTT1257350.039.60247420-21
CGCAGAG1280800.039.29009216-17
GCAGAGT1384700.038.32588216-17
GTACTTT1226250.038.21410422-23
Produced by FastQC (version 0.11.5)