##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660667_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28959818
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19104640091315	34.0	31.0	34.0	30.0	34.0
2	32.302757807386776	34.0	31.0	34.0	30.0	34.0
3	32.30554836359814	34.0	31.0	34.0	30.0	34.0
4	35.599976491564966	37.0	37.0	37.0	35.0	37.0
5	35.57475423360741	37.0	37.0	37.0	35.0	37.0
6	35.57991949396919	37.0	37.0	37.0	35.0	37.0
7	35.53496037164322	37.0	36.0	37.0	35.0	37.0
8	35.545341548762494	37.0	37.0	37.0	35.0	37.0
9	37.228106716692764	39.0	38.0	39.0	35.0	39.0
10-11	37.188029893696154	39.0	38.0	39.0	34.0	39.0
12-13	37.22490348523599	39.0	38.0	39.0	34.5	39.0
14-15	38.69847103320885	41.0	39.0	41.0	35.0	41.0
16-17	38.65583761610657	41.0	39.0	41.0	35.0	41.0
18-19	38.54247255628471	41.0	39.0	41.0	34.0	41.0
20-21	38.46568320974946	40.5	39.0	41.0	34.0	41.0
22-23	38.39683213824065	40.5	38.5	41.0	34.0	41.0
24-25	38.39223937802372	40.0	38.0	41.0	34.0	41.0
26-27	38.36664664121854	40.0	38.0	41.0	34.0	41.0
28-29	38.2829236875729	40.0	38.0	41.0	33.5	41.0
30-31	38.162245598366674	40.0	38.0	41.0	33.0	41.0
32-33	38.07232315824636	40.0	38.0	41.0	33.0	41.0
34-35	37.97928301897477	40.0	38.0	41.0	33.0	41.0
36-37	37.84669442673984	40.0	38.0	41.0	33.0	41.0
38-39	37.553188697525655	40.0	37.0	41.0	32.0	41.0
40-41	37.43809589894522	40.0	36.0	41.0	32.0	41.0
42-43	37.269673258996306	40.0	36.0	41.0	31.0	41.0
44-45	37.13956619133449	40.0	35.5	41.0	31.0	41.0
46-47	37.03989277487862	40.0	35.0	41.0	30.5	41.0
48-49	37.01340750829304	40.0	35.0	41.0	30.0	41.0
50-51	36.30090092417017	39.0	34.5	40.5	29.5	41.0
52-53	36.22904793462445	39.0	35.0	40.0	29.5	41.0
54-55	36.42120896616132	39.0	35.0	41.0	29.0	41.0
56-57	36.26823008694322	39.0	35.0	41.0	28.0	41.0
58-59	36.06985485889449	39.0	35.0	41.0	28.0	41.0
60-61	35.84999406764227	39.0	35.0	41.0	28.0	41.0
62-63	35.57315814277562	38.0	35.0	41.0	27.0	41.0
64-65	35.26250498190286	37.5	35.0	40.0	26.5	41.0
66-67	34.908430432815564	37.0	34.5	40.0	26.0	41.0
68-69	34.5837549289847	36.5	34.0	39.5	26.0	41.0
70-71	34.271776069172816	36.0	34.0	39.0	26.0	41.0
72-73	33.938909578091966	35.0	34.0	39.0	25.0	41.0
74-75	33.608700683132746	35.0	34.0	38.0	25.0	40.5
76-77	33.27362449239149	35.0	33.5	37.0	25.0	39.5
78-79	32.94524587481869	35.0	33.0	37.0	24.0	39.0
80-81	32.65405212491322	35.0	33.0	36.0	24.0	39.0
82-83	32.3537356139462	35.0	33.0	36.0	23.5	37.5
84-85	32.09771397734613	35.0	33.0	35.5	23.0	37.0
86-87	31.880530067557746	35.0	33.0	35.0	22.5	37.0
88-89	31.680733974225944	35.0	32.5	35.0	20.5	36.0
90-91	31.50349125122264	35.0	32.0	35.0	20.0	36.0
92-93	31.373408596697672	35.0	32.0	35.0	20.0	36.0
94-95	31.247306112213828	35.0	32.0	35.0	20.0	35.5
96-97	31.148293766901435	35.0	32.0	35.0	19.5	35.0
98-99	30.969258853767656	35.0	31.5	35.0	18.5	35.0
100	30.876242385224934	35.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.13181420044548986
1101	2	-0.13303392296425898
1101	3	-0.13007689732508965
1101	4	-0.12363883312528401
1101	5	-0.13947944539435042
1101	6	-0.11448472728853432
1101	7	-0.13950925801201208
1101	8	-0.1296294764646575
1101	9	-0.15435304542606332
1101	10-11	-0.1816578240917437
1101	12-13	-0.21979988978938536
1101	14-15	-0.19803142712962796
1101	16-17	-0.19417442324929368
1101	18-19	-0.1690657338870949
1101	20-21	-0.18639096005269806
1101	22-23	-0.11985456235422731
1101	24-25	-0.12087123626523777
1101	26-27	-0.12455266706007961
1101	28-29	-0.16199730466363604
1101	30-31	-0.14552922543690272
1101	32-33	-0.16895787916292448
1101	34-35	-0.18254670392822447
1101	36-37	-0.14222474056189327
1101	38-39	-0.07939456518894161
1101	40-41	-0.03920928157263148
1101	42-43	-0.02894736834600309
1101	44-45	-0.04870284459291696
1101	46-47	-0.04622591548430677
1101	48-49	-0.10915844616147297
1101	50-51	0.02670285024074559
1101	52-53	-0.02735520920735013
1101	54-55	-0.05314418737475535
1101	56-57	-0.0016814401785296695
1101	58-59	0.01373583413957391
1101	60-61	0.008313681248836247
1101	62-63	-0.008539066319123378
1101	64-65	-6.298207829260605E-4
1101	66-67	-0.0230984767392286
1101	68-69	0.010448221882079167
1101	70-71	0.022973091337213702
1101	72-73	0.02377991711848182
1101	74-75	0.03564114289805076
1101	76-77	0.02912684107828767
1101	78-79	0.014903181888087147
1101	80-81	0.01990546251786185
1101	82-83	0.0023222686186201713
1101	84-85	-0.030906011771399733
1101	86-87	-0.023301795013143334
1101	88-89	-0.07353213966634442
1101	90-91	-0.05446380420661612
1101	92-93	-0.03465243534591522
1101	94-95	-0.04381399865842184
1101	96-97	-0.024993722615690217
1101	98-99	-0.020949566396293307
1101	100	-0.07986883679399881
1102	1	-0.12597683985212882
1102	2	-0.11279442395628081
1102	3	-0.13268259842754304
1102	4	-0.13903830919054627
1102	5	-0.10971723062664296
1102	6	-0.16094118493161602
1102	7	-0.14602834214066718
1102	8	-0.1587199483968007
1102	9	-0.1927473582500312
1102	10-11	-0.14997915805165007
1102	12-13	-0.231575364657715
1102	14-15	-0.2510696931528713
1102	16-17	-0.2133607692024242
1102	18-19	-0.18772885004214857
1102	20-21	-0.16201278867151814
1102	22-23	-0.14856227993935534
1102	24-25	-0.1676962659384671
1102	26-27	-0.15541130902632005
1102	28-29	-0.15041560573167345
1102	30-31	-0.1841796127997526
1102	32-33	-0.16433522900872077
1102	34-35	-0.18114418843373414
1102	36-37	-0.12247221580127388
1102	38-39	-0.06332188267095518
1102	40-41	-0.0865472926468911
1102	42-43	-0.12681800724049452
1102	44-45	-0.09267491109751802
1102	46-47	-0.06433668591142805
1102	48-49	-0.1458138197551193
1102	50-51	-0.020258816350136044
1102	52-53	-0.02701808648408388
1102	54-55	-0.0800942181990294
1102	56-57	-0.053699141962319175
1102	58-59	0.014804383380159436
1102	60-61	-0.060876063639739186
1102	62-63	-0.03722008340425731
1102	64-65	-0.016933934814218787
1102	66-67	-0.015715301044842533
1102	68-69	-0.017530922059670218
1102	70-71	-0.0012774663722012747
1102	72-73	0.014273370892311732
1102	74-75	0.02905157744954323
1102	76-77	0.011173023846701824
1102	78-79	0.029351961176097063
1102	80-81	-0.01216374981002133
1102	82-83	0.02848908836610775
1102	84-85	-0.018410852656927545
1102	86-87	-0.04342929089808578
1102	88-89	-0.08670141191718628
1102	90-91	-0.0623067310744716
1102	92-93	-0.08491691506783638
1102	94-95	-0.06017681173764444
1102	96-97	-0.08301853124846659
1102	98-99	-0.15007624495516225
1102	100	-0.10701739441283209
1103	1	-0.24666327619640782
1103	2	-0.254972805081799
1103	3	-0.2483146854316729
1103	4	-0.2422904295248003
1103	5	-0.26441295019114364
1103	6	-0.24437824745095327
1103	7	-0.24234985066235737
1103	8	-0.26546233152406273
1103	9	-0.2663238586822416
1103	10-11	-0.26303245897496197
1103	12-13	-0.2810193041295932
1103	14-15	-0.32199784018249034
1103	16-17	-0.31451866436740517
1103	18-19	-0.31422532466509523
1103	20-21	-0.3063273105677027
1103	22-23	-0.29616107858860374
1103	24-25	-0.2713003764766242
1103	26-27	-0.28036705886709967
1103	28-29	-0.2437972704959961
1103	30-31	-0.22746262595345712
1103	32-33	-0.237975860497464
1103	34-35	-0.21721043807487206
1103	36-37	-0.23117425997847363
1103	38-39	-0.17383082899184643
1103	40-41	-0.1555133442510268
1103	42-43	-0.15126889015187572
1103	44-45	-0.16971176235603025
1103	46-47	-0.19760403453771147
1103	48-49	-0.2114744352080038
1103	50-51	-0.11144778170111636
1103	52-53	-0.09967949174983204
1103	54-55	-0.18915019399831579
1103	56-57	-0.15580269663193036
1103	58-59	-0.14802252728626542
1103	60-61	-0.17471705060440001
1103	62-63	-0.14395489313665877
1103	64-65	-0.15410753849116787
1103	66-67	-0.16811681023644098
1103	68-69	-0.15357756043403015
1103	70-71	-0.12517092152224762
1103	72-73	-0.10907948022411773
1103	74-75	-0.12005167265775896
1103	76-77	-0.11426003675344276
1103	78-79	-0.11613831948306341
1103	80-81	-0.11874880301636637
1103	82-83	-0.11585590908686783
1103	84-85	-0.09965255967917841
1103	86-87	-0.15319300633435162
1103	88-89	-0.13188187637179638
1103	90-91	-0.11548272623332423
1103	92-93	-0.13091017941207994
1103	94-95	-0.16070987141426585
1103	96-97	-0.12574629389376568
1103	98-99	-0.1278634150069955
1103	100	-0.13435826616869662
1104	1	-0.19622403175433334
1104	2	-0.2214428094448877
1104	3	-0.23095700498581095
1104	4	-0.23306731021729377
1104	5	-0.23426643200271258
1104	6	-0.25107582029547615
1104	7	-0.23648282877528004
1104	8	-0.22861550173821854
1104	9	-0.2510017739023951
1104	10-11	-0.259607457682165
1104	12-13	-0.3172840110978328
1104	14-15	-0.32146870627950364
1104	16-17	-0.31839393595011956
1104	18-19	-0.27804683918848383
1104	20-21	-0.26559192891979677
1104	22-23	-0.26258110797790124
1104	24-25	-0.2972474882554721
1104	26-27	-0.296675113439683
1104	28-29	-0.2539683545946403
1104	30-31	-0.2143834705596106
1104	32-33	-0.24081271296621054
1104	34-35	-0.23994934040133842
1104	36-37	-0.23359157929068886
1104	38-39	-0.18768774801424115
1104	40-41	-0.17882486158143962
1104	42-43	-0.20572443554239328
1104	44-45	-0.21569007902213855
1104	46-47	-0.21552074217276385
1104	48-49	-0.2698623792286554
1104	50-51	-0.1322923361899484
1104	52-53	-0.14670572690508976
1104	54-55	-0.23000211469167198
1104	56-57	-0.18810990848621856
1104	58-59	-0.1936017797155145
1104	60-61	-0.17545270532124846
1104	62-63	-0.16229713743209828
1104	64-65	-0.1730886238545466
1104	66-67	-0.1621301153492638
1104	68-69	-0.18123790700632014
1104	70-71	-0.17596757790457218
1104	72-73	-0.1838689831789253
1104	74-75	-0.19053541351772196
1104	76-77	-0.1513866304752156
1104	78-79	-0.14936084721244214
1104	80-81	-0.16035178754480484
1104	82-83	-0.1438907503478859
1104	84-85	-0.16619059818947335
1104	86-87	-0.17419181514936355
1104	88-89	-0.158716243294311
1104	90-91	-0.13555803104280528
1104	92-93	-0.13201778555601962
1104	94-95	-0.1663675746652835
1104	96-97	-0.13938759918016785
1104	98-99	-0.15373806094079256
1104	100	-0.10469380693092134
1105	1	-0.3051134597596885
1105	2	-0.32683556799678115
1105	3	-0.31017102179849587
1105	4	-0.3209444653622171
1105	5	-0.29786083265424423
1105	6	-0.323842239671265
1105	7	-0.3137433678904813
1105	8	-0.28646716439445896
1105	9	-0.38744617309235707
1105	10-11	-0.3490151119285798
1105	12-13	-0.3732523257041933
1105	14-15	-0.42960414080727105
1105	16-17	-0.42496922204453114
1105	18-19	-0.38461668188934084
1105	20-21	-0.3866958856344169
1105	22-23	-0.3092952258741448
1105	24-25	-0.34761041536003034
1105	26-27	-0.31917985339372734
1105	28-29	-0.3282889580238191
1105	30-31	-0.3196017671288587
1105	32-33	-0.3297881674854466
1105	34-35	-0.346254782733979
1105	36-37	-0.32047905486594885
1105	38-39	-0.2727751325441474
1105	40-41	-0.2555074509182944
1105	42-43	-0.2617609215252088
1105	44-45	-0.28111243294088695
1105	46-47	-0.2937072115350361
1105	48-49	-0.35351682651344163
1105	50-51	-0.18524435003848083
1105	52-53	-0.21356415758651082
1105	54-55	-0.2546552816122727
1105	56-57	-0.22314315329019507
1105	58-59	-0.24333721690832277
1105	60-61	-0.24470417570152136
1105	62-63	-0.2258104391164224
1105	64-65	-0.25297829860956966
1105	66-67	-0.22286485723952865
1105	68-69	-0.22504447149960782
1105	70-71	-0.20511185165938883
1105	72-73	-0.22982127498190863
1105	74-75	-0.1955623204071344
1105	76-77	-0.2032284846539696
1105	78-79	-0.20298616682705273
1105	80-81	-0.1739804032636414
1105	82-83	-0.14927320818720347
1105	84-85	-0.11780589493343285
1105	86-87	-0.15084170792313856
1105	88-89	-0.11497669425354573
1105	90-91	-0.1448932202719746
1105	92-93	-0.16658712033311573
1105	94-95	-0.15293019800447638
1105	96-97	-0.13712523281416722
1105	98-99	-0.12352867056620553
1105	100	-0.10191457093307932
1106	1	-0.3025167513429068
1106	2	-0.29557012144997685
1106	3	-0.30967206311536444
1106	4	-0.29719530453481013
1106	5	-0.29690304221840336
1106	6	-0.35535524721944256
1106	7	-0.3142668468601073
1106	8	-0.30939598381373656
1106	9	-0.345651388548724
1106	10-11	-0.3744420326525244
1106	12-13	-0.4383233305284122
1106	14-15	-0.48217792350486377
1106	16-17	-0.4427260691685504
1106	18-19	-0.3922290484824842
1106	20-21	-0.41155320011244356
1106	22-23	-0.37492926778276114
1106	24-25	-0.3443395850597568
1106	26-27	-0.3505040913698636
1106	28-29	-0.3636525051881918
1106	30-31	-0.34192437456668756
1106	32-33	-0.412154434481387
1106	34-35	-0.3457180138199192
1106	36-37	-0.30020289262715494
1106	38-39	-0.27635676079543714
1106	40-41	-0.30820376065030075
1106	42-43	-0.30775977544077904
1106	44-45	-0.26668908001326486
1106	46-47	-0.30218492754537607
1106	48-49	-0.3110774834320864
1106	50-51	-0.1797178407882427
1106	52-53	-0.21728836315296007
1106	54-55	-0.28797621225314174
1106	56-57	-0.22476506236478855
1106	58-59	-0.19405654908831593
1106	60-61	-0.2407203881943829
1106	62-63	-0.21162397389191057
1106	64-65	-0.2396324821710678
1106	66-67	-0.26005311885919014
1106	68-69	-0.2092904275706715
1106	70-71	-0.18991341065802203
1106	72-73	-0.1872409597944582
1106	74-75	-0.177560722927943
1106	76-77	-0.14268965930182986
1106	78-79	-0.16285369139529848
1106	80-81	-0.1402616392852778
1106	82-83	-0.15908347797594757
1106	84-85	-0.1514576149468141
1106	86-87	-0.17345446266703846
1106	88-89	-0.1868271500466534
1106	90-91	-0.2253604604384165
1106	92-93	-0.1892703879698736
1106	94-95	-0.21946546238607567
1106	96-97	-0.20005841277044212
1106	98-99	-0.1804256730335574
1106	100	-0.15650750697954763
1107	1	-0.26741493200425026
1107	2	-0.2663793747101266
1107	3	-0.27490338744787124
1107	4	-0.2940804216498094
1107	5	-0.29549130299508164
1107	6	-0.327137804037541
1107	7	-0.27779578488569
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2316	96-97	0.1347138617027035
2316	98-99	0.26473940076615676
2316	100	0.07562965708315161
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1051.0
3	140.0
4	145.0
5	165.0
6	711.0
7	5559.0
8	13087.0
9	19778.0
10	27674.0
11	32711.0
12	37796.0
13	44586.0
14	57791.0
15	83205.0
16	109945.0
17	126940.0
18	128071.0
19	135943.0
20	145583.0
21	161660.0
22	195310.0
23	250562.0
24	311272.0
25	363123.0
26	401198.0
27	421226.0
28	343305.0
29	347970.0
30	391532.0
31	456746.0
32	566497.0
33	735744.0
34	1031988.0
35	2041430.0
36	3025038.0
37	5986616.0
38	8144661.0
39	2804456.0
40	8603.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.25158662588819	33.983067675157336	27.270651048100962	21.494694650853507
2	21.174941131993577	31.608449485479458	28.094618700501282	19.12199068202568
3	22.22616771156869	28.72753638020859	27.1570235386543	21.889272369568417
4	18.85505661962307	28.87515239796823	27.568033421746645	24.701757560662056
5	20.517749143630123	31.919331036038876	26.729752396563683	20.83316742376732
6	24.75084730579154	28.25823914677178	26.918904306011076	20.072009241425608
7	20.979478200577343	28.716381741699287	30.09720386742023	20.20693619030314
8	23.434103346202185	29.243045347974405	26.75398798439483	20.568863321428584
9	23.428560529351422	28.854183857125758	27.265588481072456	20.451667132450364
10-11	20.28261510915189	31.099676389136622	28.30447347599615	20.31323502571534
12-13	20.363043007955326	28.887199291041547	28.13256261134273	22.61719508966039
14-15	20.374536622182706	32.5834771135081	26.65505886543228	20.386927398876914
16-17	22.422375369249263	28.70221918439216	26.312023811642405	22.56338163471618
18-19	20.585319095543895	30.603769435610275	26.651292131985464	22.159619336860366
20-21	22.106395119774945	30.972441272044716	26.65142682773026	20.269736780450078
22-23	22.759159451742395	28.770567569048655	28.17261986155436	20.297653117654594
24-25	20.5812571402534	30.873629854829964	26.263377255177478	22.28173574973916
26-27	20.31271454142322	29.49103601426716	29.889667373940387	20.30658207036924
28-29	20.82172510557384	29.12450313235898	29.777050119299396	20.27672164276778
30-31	20.798099548846952	28.75010105638879	30.100325015319612	20.351474379444646
32-33	20.754680207188326	28.8516689226965	30.042440061920058	20.35121080819512
34-35	20.785987545174812	29.27972213727477	29.62364400849084	20.310646309059575
36-37	20.41153817797055	29.260781426102948	29.981491732310424	20.346188663616076
38-39	20.51471102179632	28.89373645411161	29.92381220035102	20.667740323741054
40-41	20.999002979590106	28.972816751269665	29.45976945250533	20.5684108166349
42-43	21.084627785798023	28.99715096988915	29.722700470912912	20.195520773399913
44-45	21.51531056218294	29.058336953863083	29.266173811782203	20.16017867217177
46-47	20.86095928835289	29.148719890974277	29.484779961083657	20.505540859589182
48-49	21.296195372125606	29.153589311720484	29.00164298410009	20.548572332053812
50-51	21.493485129278888	29.15859081594189	28.923290245781473	20.424633808997747
52-53	21.290879138553706	29.622069739907854	29.10210164793559	19.984949473602846
54-55	21.581800275576526	29.39214981637154	28.656865780693373	20.36918412735856
56-57	21.928710068046197	29.91328323072555	28.25623676510356	19.90176993612469
58-59	22.207350022091287	30.15522442753259	27.882052126458007	19.755373423918115
60-61	22.488402961786306	30.594509858261752	27.28426713051078	19.63282004944116
62-63	22.74210219202327	30.6287342799506	27.135310145981823	19.49385338204431
64-65	23.03121692485742	30.660875628414757	27.00609007449726	19.301817372230566
66-67	23.28361528217152	30.587153704387077	26.95923474855958	19.16999626488182
68-69	23.540945608619086	30.424377220250605	26.969078770887677	19.065598400242635
70-71	23.867236742784794	30.24528555044182	26.941956414208622	18.945521292564763
72-73	24.158337148033958	30.07890968386473	26.892180887015694	18.87057228108562
74-75	24.475453882759375	29.96969532426355	26.798567686636925	18.756283106340142
76-77	24.807530215595587	29.912035524572083	26.638220223304533	18.642214036527793
78-79	25.082142136352164	29.891193986489263	26.451268699436064	18.57539517772251
80-81	25.384917210397003	29.810816211174323	26.287497187155083	18.51676939127359
82-83	25.591376693942824	29.81663542391797	26.138551523178773	18.453436358960435
84-85	25.780269998281717	29.694902601405744	26.062120602746553	18.462706797565986
86-87	25.987073048478088	29.572656339350594	26.00013685564248	18.440133756528837
88-89	26.08264153015274	29.50746040704239	26.01012544693453	18.399772615870337
90-91	26.138186632160913	29.453491456142494	25.990740309449546	18.417581602247047
92-93	26.18305826128801	29.48564641126129	25.959167357332436	18.372127970118264
94-95	26.184198331892738	29.59863096889352	25.89263344911645	18.324537250097297
96-97	26.169886009944253	29.71458298248047	25.78275515285452	18.332775854720758
98-99	26.19445160629693	29.878591139244865	25.640021341984205	18.286935912474004
100	26.146025945366585	30.104979916274317	25.501176013964667	18.24781812439443
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	526963.0
1	306093.0
2	63497.0
3	37539.5
4	31146.5
5	27347.0
6	25200.5
7	23997.5
8	22886.0
9	22499.5
10	23136.5
11	29899.0
12	175397.0
13	191120.5
14	60786.0
15	55651.0
16	58937.0
17	62660.5
18	66418.0
19	71893.0
20	81331.0
21	94372.5
22	112234.5
23	134981.0
24	160326.5
25	189425.5
26	221887.5
27	257899.0
28	300194.5
29	347924.5
30	398603.0
31	450904.0
32	508218.0
33	568875.0
34	619969.5
35	673947.5
36	749637.0
37	803132.0
38	832279.5
39	860610.0
40	873069.0
41	872664.0
42	867577.0
43	873869.5
44	891028.0
45	912120.0
46	934836.5
47	955451.0
48	969975.5
49	967499.0
50	957445.0
51	946334.5
52	924655.0
53	889708.5
54	843241.0
55	791253.0
56	731821.0
57	666794.0
58	603141.0
59	529306.5
60	437724.0
61	353131.5
62	283022.5
63	215852.0
64	161971.0
65	122699.5
66	90355.0
67	66982.0
68	50784.5
69	38524.0
70	30318.0
71	25705.0
72	22665.5
73	18077.0
74	12969.5
75	9584.5
76	7370.5
77	5473.5
78	3917.5
79	2786.5
80	2005.0
81	1555.5
82	1192.5
83	956.0
84	828.0
85	704.0
86	634.0
87	567.0
88	477.5
89	418.5
90	378.5
91	350.5
92	294.0
93	243.5
94	231.5
95	219.0
96	198.5
97	167.0
98	125.5
99	96.0
100	90.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06016957703256284
2	0.07665103420194146
3	0.0628077151589834
4	0.08592940742928702
5	0.0980461962847971
6	0.08893011689507165
7	0.07766623395216089
8	0.07234506791444616
9	0.06680290601273806
10-11	0.06734848955197163
12-13	0.06676492234861421
14-15	0.06538887778921815
16-17	0.07262821886518762
18-19	0.07270763925381023
20-21	0.14719360460069192
22-23	0.12621971588357359
24-25	0.11899764010947858
26-27	0.10993853621593894
28-29	0.11690335899210416
30-31	0.09651649053871816
32-33	0.07660096482650547
34-35	0.053361868503455374
36-37	0.05337050115439262
38-39	0.058684761071357554
40-41	0.06071170751142152
42-43	0.04107933274995029
44-45	0.027705629917978074
46-47	0.020431758238259646
48-49	0.01790757110421067
50-51	0.021450411048853965
52-53	0.018470419945318717
54-55	0.020625129619253824
56-57	0.0233685860871087
58-59	0.021048129515178583
60-61	0.022142749654020615
62-63	0.023700079883098715
64-65	0.023501528911542193
66-67	0.02157990081291257
68-69	0.025647605934540057
70-71	0.029280225448930653
72-73	0.03216698392234371
74-75	0.03166456363779634
76-77	0.031215665789059863
78-79	0.029271592797993413
80-81	0.02697876070906247
82-83	0.03050260882164384
84-85	0.03237071448446257
86-87	0.033166644900876105
88-89	0.033454975442179916
90-91	0.03205303292997214
92-93	0.030689074081888226
94-95	0.03453405680933492
96-97	0.03290593884257145
98-99	0.14884934705045452
100	0.0371445704527563
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.8959818E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.73857722761667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2418376966353	38.41899109477128
2	12.218387714026704	12.154504418221599
3	4.148184800641257	6.189744301833625
4	1.910458165350996	3.800938839897655
5	1.0956526662475987	2.724810237740015
6	0.6676485063590806	1.9924732076666432
7	0.4750796451621111	1.6540849934115074
8	0.33514480671776314	1.33357006810929
9	0.25969950054438357	1.162537529742027
>10	1.4847437140771802	13.38149705201169
>50	0.09239710670480643	3.136242561590879
>100	0.06845542686554121	7.344697082045395
>500	0.0018899259678889107	0.5493367490419236
>1k	3.4611523586414584E-4	0.3228945425405978
>5k	4.047788919148464E-5	0.13009355026226188
>10k+	3.373157427231154E-5	5.703583771113634
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	884105	3.0528679427474303	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	449881	1.5534662545185884	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	186623	0.6444204863442167	Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	161722	0.5584358299489313	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.7231226384088464	0.0	0.0	6.906120749792005E-6	6.906120749792005E-6
2	0.7231709812540948	3.4530603748960025E-6	0.0	6.906120749792005E-6	6.906120749792005E-6
3	0.7231813404352195	3.4530603748960025E-6	0.0	6.906120749792005E-6	1.726530187448001E-5
4	0.7231813404352195	1.0359181124688007E-5	0.0	1.0359181124688007E-5	1.726530187448001E-5
5	0.7231813404352195	1.381224149958401E-5	0.0	1.0359181124688007E-5	2.0718362249376014E-5
6	0.723267666944592	1.381224149958401E-5	0.0	1.0359181124688007E-5	2.4171422624272017E-5
7	0.7232780261257167	1.726530187448001E-5	0.0	1.726530187448001E-5	2.4171422624272017E-5
8	0.7232952914275912	1.726530187448001E-5	0.0	1.726530187448001E-5	2.4171422624272017E-5
9	0.7233470873332146	1.726530187448001E-5	0.0	1.726530187448001E-5	3.107754337406402E-5
10-11	0.7233712587558389	2.589795281172002E-5	0.0	1.726530187448001E-5	3.280407356151202E-5
12-13	0.7234213281312749	3.280407356151202E-5	0.0	1.726530187448001E-5	3.625713393640803E-5
14-15	0.7235663566670205	3.7983664123856026E-5	0.0	1.726530187448001E-5	4.488978487364803E-5
16-17	0.7237597280480147	3.7983664123856026E-5	0.0	1.8991832061928013E-5	5.3522435810888036E-5
18-19	0.7240618708308181	4.8342845248544035E-5	0.0	2.0718362249376014E-5	5.8702026373232043E-5
20-21	0.7244831441965554	5.697549618578404E-5	0.0	2.4171422624272017E-5	6.560814712302404E-5
22-23	0.7250874297621622	7.078773768536806E-5	0.0	2.9351013186616022E-5	7.424079806026405E-5
24-25	0.7270884782494145	7.251426787281606E-5	0.0	3.9710194311304024E-5	8.459997918495207E-5
26-27	0.7335163501372833	7.424079806026405E-5	0.0	5.6975496185784045E-5	8.977956974729606E-5
28-29	0.751705345662048	7.942038862260806E-5	0.0	6.215508674812804E-5	9.495916030964007E-5
30-31	0.7937826128603434	7.942038862260806E-5	0.0	6.906120749792005E-5	1.0186528105943208E-4
32-33	0.8743597767085415	8.287344899750406E-5	0.0	6.906120749792005E-5	1.1049793199667208E-4
34-35	1.0072369929949145	8.287344899750406E-5	0.0	7.596732824771205E-5	1.1395099237156809E-4
36-37	1.2030393284930174	8.459997918495207E-5	0.0	7.769385843516005E-5	1.1913058293391208E-4
38-39	1.4661193658054068	8.977956974729606E-5	1.7265301874480013E-6	8.287344899750406E-5	1.329428244334961E-4
40-41	1.7974664067294899	9.495916030964007E-5	3.4530603748960025E-6	8.977956974729606E-5	1.415754753707361E-4
42-43	2.1932406481283824	1.0013875087198407E-4	3.4530603748960025E-6	9.668569049708807E-5	1.519346564954241E-4
44-45	2.65559679967602	1.1395099237156808E-4	3.4530603748960025E-6	9.668569049708807E-5	1.588407772452161E-4
46-47	3.190277300775854	1.1395099237156808E-4	3.4530603748960025E-6	9.668569049708807E-5	1.691999583699041E-4
48-49	3.791881565001548	1.2085711312136008E-4	3.4530603748960025E-6	1.1222446218412008E-4	1.7265301874480013E-4
50-51	4.451186468091754	1.2603670368370408E-4	3.4530603748960025E-6	1.1740405274646409E-4	1.8128566968204012E-4
52-53	5.163861872336352	1.277632338711521E-4	3.4530603748960025E-6	1.2258364330880808E-4	1.8819179043183214E-4
54-55	5.924800701440873	1.294897640586001E-4	3.4530603748960025E-6	1.277632338711521E-4	2.0027750174396816E-4
56-57	6.731421792775079	1.312162942460481E-4	3.4530603748960025E-6	1.312162942460481E-4	2.1236321305610414E-4
58-59	7.586907141474439	1.312162942460481E-4	3.4530603748960025E-6	1.363958848083921E-4	2.1926933380589616E-4
60-61	8.480295007378846	1.346693546209441E-4	3.4530603748960025E-6	1.381224149958401E-4	2.2099586399334416E-4
62-63	9.397448561313471	1.381224149958401E-4	3.4530603748960025E-6	1.450285357456321E-4	2.2444892436824016E-4
64-65	10.339914774326274	1.398489451832881E-4	3.4530603748960025E-6	1.467550659330801E-4	2.3135504511803218E-4
66-67	11.304539275764785	1.450285357456321E-4	3.4530603748960025E-6	1.571142470577681E-4	2.3480810549292817E-4
68-69	12.285415605857743	1.484815961205281E-4	3.4530603748960025E-6	1.6574689799500811E-4	2.3826116586782417E-4
70-71	13.285800000538678	1.519346564954241E-4	3.4530603748960025E-6	1.6574689799500811E-4	2.4171422624272017E-4
72-73	14.292360193700112	1.519346564954241E-4	3.4530603748960025E-6	1.6574689799500811E-4	2.4862034699251216E-4
74-75	15.308264368236017	1.6229383762011212E-4	3.4530603748960025E-6	1.6574689799500811E-4	2.555264677423042E-4
76-77	16.334008038310188	1.6229383762011212E-4	3.4530603748960025E-6	1.674734281824561E-4	2.607060583046482E-4
78-79	17.363601870702364	1.6229383762011212E-4	3.4530603748960025E-6	1.7437954893224813E-4	2.658856488669922E-4
80-81	18.398661897668006	1.7610607911969613E-4	3.4530603748960025E-6	1.7955913949459213E-4	2.693387092418882E-4
82-83	19.436246111767694	1.9337138099417614E-4	3.4530603748960025E-6	1.7955913949459213E-4	2.693387092418882E-4
84-85	20.47629235791468	2.1236321305610414E-4	3.4530603748960025E-6	1.8128566968204012E-4	2.8487748092892017E-4
86-87	21.515468432847197	2.2272239418079216E-4	3.4530603748960025E-6	1.8991832061928014E-4	2.866040111163682E-4
88	22.292947421147467	2.3826116586782417E-4	3.4530603748960025E-6	1.8991832061928014E-4	2.900570714912642E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	126275	0.0	80.647606	8
GTGGTAT	127530	0.0	80.04534	6
AGTGGTA	128225	0.0	79.6186	5
AAGCAGT	130285	0.0	79.11544	1
GTATCAA	128965	0.0	78.72973	9
TGGTATC	131085	0.0	77.72038	7
GCAGTGG	133920	0.0	76.80935	3
CAGTGGT	135605	0.0	75.49782	4
AGCAGTG	136955	0.0	75.46832	2
CAACGCA	123440	0.0	43.0299	12-13
ATCAACG	124920	0.0	42.612427	10-11
ACGCAGA	126390	0.0	42.011078	14-15
TCAACGC	122900	0.0	41.121403	12-13
AGAGTAC	128370	0.0	41.026432	18-19
GAGTACT	116510	0.0	40.445072	20-21
AACGCAG	125185	0.0	40.262375	14-15
AGTACTT	125735	0.0	39.602474	20-21
CGCAGAG	128080	0.0	39.290092	16-17
GCAGAGT	138470	0.0	38.325882	16-17
GTACTTT	122625	0.0	38.214104	22-23
>>END_MODULE