FastQCFastQC Report
Thu 26 May 2016
SRR1660667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28959818
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14459794.993052787831747No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2865050.9893190627095791No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2647490.9141942811933418No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACACGAATCTCGTATG1177630.406642748928878TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACACACGAATCTCGTATG721300.24906924484124865TruSeq Adapter, Index 5 (97% over 36bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT294210.10159248928981529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1952350.084.596451
GGTATCA1952200.083.576918
GTGGTAT1961100.083.321486
AGTGGTA1968200.083.130735
GTATCAA1978300.082.205899
TGGTATC1998800.081.5805367
GCAGTGG2020650.081.409133
AGCAGTG2048850.080.7355352
CAGTGGT2038150.080.475774
CAACGCA1927150.044.28235612-13
ATCAACG1940050.044.04963310-11
ACGCAGA1961700.043.5215314-15
AGAGTAC1980150.042.737118-19
TCAACGC1924350.042.1724812-13
AGTACTT1954800.041.7531220-21
AACGCAG1946900.041.54276714-15
GAGTACT1862000.041.4429220-21
GCAGAGT2077000.041.06716-17
CGCAGAG1973750.040.89662616-17
TATCAAC2034400.039.9962310-11
Produced by FastQC (version 0.11.5)