##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660667_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28959818
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.693427907592515	34.0	31.0	34.0	31.0	34.0
2	32.92584100494002	34.0	31.0	34.0	31.0	34.0
3	32.997766560549515	34.0	33.0	34.0	31.0	34.0
4	36.37339664910878	37.0	37.0	37.0	35.0	37.0
5	36.30375712305927	37.0	37.0	37.0	35.0	37.0
6	36.299775571793994	37.0	37.0	37.0	35.0	37.0
7	36.21157857414712	37.0	37.0	37.0	35.0	37.0
8	36.254938100785026	37.0	37.0	37.0	35.0	37.0
9	38.07092813912021	39.0	39.0	39.0	35.0	39.0
10-11	37.965656500327455	39.0	38.0	39.0	35.0	39.0
12-13	38.060786259084914	39.0	39.0	39.0	35.0	39.0
14-15	39.61041678507786	41.0	40.0	41.0	37.0	41.0
16-17	39.53848732751014	41.0	39.5	41.0	37.0	41.0
18-19	39.43124601128363	41.0	39.0	41.0	36.0	41.0
20-21	39.34980418385226	41.0	39.0	41.0	36.0	41.0
22-23	39.19925391105704	41.0	39.0	41.0	35.5	41.0
24-25	39.2216503915874	41.0	39.0	41.0	35.0	41.0
26-27	39.19645358268481	41.0	39.0	41.0	35.0	41.0
28-29	39.138502959514454	41.0	39.0	41.0	35.0	41.0
30-31	39.05577032631904	41.0	39.0	41.0	35.0	41.0
32-33	38.96373924035019	41.0	39.0	41.0	35.0	41.0
34-35	38.87044751109969	40.0	38.5	41.0	35.0	41.0
36-37	38.72125821025533	40.0	38.0	41.0	35.0	41.0
38-39	38.33217347912891	40.0	37.5	41.0	35.0	41.0
40-41	38.218338941218484	40.0	37.0	41.0	34.0	41.0
42-43	38.05669445160187	40.0	37.0	41.0	34.0	41.0
44-45	37.983345699893555	40.0	37.0	41.0	34.0	41.0
46-47	37.887716732197696	40.0	36.0	41.0	34.0	41.0
48-49	37.82147268674133	40.0	36.0	41.0	33.0	41.0
50-51	37.7113435070621	40.0	36.0	41.0	33.0	41.0
52-53	37.45209243027702	40.0	35.5	41.0	33.0	41.0
54-55	36.92934292611922	40.0	35.0	41.0	31.0	41.0
56-57	36.57855613940668	39.5	35.0	41.0	30.5	41.0
58-59	36.38513719250584	39.0	35.0	41.0	30.0	41.0
60-61	36.170132491854744	39.0	35.0	41.0	30.0	41.0
62-63	35.95320664653349	38.5	35.0	41.0	30.0	41.0
64-65	35.66799223323848	37.5	35.0	40.0	29.0	41.0
66-67	35.39904546361444	37.0	35.0	40.0	29.0	41.0
68-69	35.111730173856756	36.5	35.0	39.5	29.0	41.0
70-71	34.79200098909462	36.0	35.0	39.0	28.5	41.0
72-73	34.41782391381051	35.5	34.0	39.0	28.0	41.0
74-75	33.98806534281397	35.0	34.0	37.5	27.0	40.0
76-77	32.771359146663144	34.5	32.5	36.5	25.5	39.0
78-79	33.27968970661348	35.0	34.0	37.0	26.0	39.0
80-81	33.07257065634874	35.0	34.0	36.0	26.0	39.0
82-83	32.854928594509815	35.0	34.0	36.0	26.0	37.5
84-85	32.71068994632494	35.0	34.0	35.5	25.5	37.0
86-87	32.51873535600258	35.0	34.0	35.0	25.0	37.0
88-89	32.34668586315011	35.0	34.0	35.0	25.0	36.0
90-91	32.19075648196408	35.0	34.0	35.0	25.0	36.0
92-93	32.03818504660492	35.0	33.0	35.0	24.0	36.0
94-95	31.936296630040978	35.0	33.0	35.0	24.0	35.5
96-97	31.836220724867815	35.0	33.0	35.0	23.0	35.0
98-99	31.72929016680975	35.0	33.0	35.0	23.0	35.0
100	31.67207677202944	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13168963503561315
1101	2	0.08215701958880572
1101	3	0.03895973040037859
1101	4	0.023766141660097162
1101	5	0.02038801434893145
1101	6	0.027628869534993328
1101	7	0.01890516049675739
1101	8	0.03492658555304473
1101	9	0.03305163367599562
1101	10-11	0.015166986489781209
1101	12-13	-0.011050813403862492
1101	14-15	-0.009160787491943267
1101	16-17	0.008207401037644502
1101	18-19	0.0019096199111316992
1101	20-21	0.03860611379238321
1101	22-23	0.049375995466625966
1101	24-25	0.04954613527984009
1101	26-27	0.03710952781467114
1101	28-29	0.03443749605677482
1101	30-31	0.02111629878363175
1101	32-33	0.0295996005015553
1101	34-35	0.049149977571069314
1101	36-37	0.04633949633413437
1101	38-39	0.05754250046383902
1101	40-41	0.06913751682949254
1101	42-43	0.031246062475624115
1101	44-45	0.07264793557160942
1101	46-47	0.0660337120256358
1101	48-49	0.07520181337642384
1101	50-51	0.03942741851006559
1101	52-53	0.0467254591452857
1101	54-55	0.0965779698129623
1101	56-57	0.06911491389347901
1101	58-59	0.04883806492056664
1101	60-61	0.009494807294487373
1101	62-63	0.012615528233759221
1101	64-65	0.031856122058130154
1101	66-67	0.01012061176645318
1101	68-69	0.029645115312575854
1101	70-71	0.012992246461458024
1101	72-73	0.010826572470783447
1101	74-75	-0.012072269902006383
1101	76-77	0.200957497129842
1101	78-79	-0.03222058847636333
1101	80-81	-0.02929983279506132
1101	82-83	-0.020347473913652436
1101	84-85	0.033267520777485515
1101	86-87	0.05384527670177164
1101	88-89	0.04770341023024827
1101	90-91	0.023871807477341633
1101	92-93	0.053331777600952535
1101	94-95	0.03127991061487023
1101	96-97	0.04243110960847574
1101	98-99	0.007728502819468019
1101	100	-0.011446027071432496
1102	1	0.11431231783043216
1102	2	0.0651787354969997
1102	3	0.007510652249337113
1102	4	0.03136272288920594
1102	5	0.024407978858171475
1102	6	4.218882580033778E-4
1102	7	0.0026880445048576007
1102	8	0.010772372211889092
1102	9	-0.03695944659303052
1102	10-11	-0.021436747162496772
1102	12-13	-0.021527836777700315
1102	14-15	-0.034066095923869
1102	16-17	0.0032581350235929563
1102	18-19	-0.01038017535767466
1102	20-21	-0.006049943982290529
1102	22-23	0.03370599536112451
1102	24-25	0.021841822638464237
1102	26-27	-0.026621441005865165
1102	28-29	-0.06683968611633873
1102	30-31	-0.004039486646455259
1102	32-33	-0.03685313005635038
1102	34-35	-0.008254880478055782
1102	36-37	-0.01538525337917207
1102	38-39	0.011064924476571036
1102	40-41	-0.02473824161523197
1102	42-43	-0.04837544186224818
1102	44-45	-0.06388631452310278
1102	46-47	0.01463901780581267
1102	48-49	-0.03932145222951533
1102	50-51	-0.05819229209574672
1102	52-53	-0.07011846478945216
1102	54-55	-2.4956903545358955E-4
1102	56-57	-0.00745832207999797
1102	58-59	-0.07308266895508808
1102	60-61	-0.04055733306651632
1102	62-63	-0.0862486287526778
1102	64-65	-0.1275952561606033
1102	66-67	-0.07804866904865548
1102	68-69	-0.10663570644664588
1102	70-71	-0.10471978655012748
1102	72-73	-0.1220819743438355
1102	74-75	-0.08206484264496794
1102	76-77	0.07811373725150617
1102	78-79	-0.10746456827592965
1102	80-81	-0.14068952125960266
1102	82-83	-0.09760757557000233
1102	84-85	-0.056294853304379444
1102	86-87	-0.0838066307326173
1102	88-89	-0.05463981805966256
1102	90-91	-0.052017944927889914
1102	92-93	-0.07196809801457249
1102	94-95	-0.06366324884379893
1102	96-97	-0.10500615235001476
1102	98-99	-0.05636141220993807
1102	100	-0.11632896856142594
1103	1	0.04703606475176514
1103	2	0.00781666060953512
1103	3	-0.018904147332044374
1103	4	-0.004655050157680307
1103	5	-0.03417715123186582
1103	6	-0.03642374906345225
1103	7	-0.05283485792487852
1103	8	-0.03504500984652026
1103	9	-0.0699708928552738
1103	10-11	-0.06028559647843679
1103	12-13	-0.06730396492576318
1103	14-15	-0.07178575991226666
1103	16-17	-0.04077909800661672
1103	18-19	-0.04277420845094326
1103	20-21	-0.03402053273076433
1103	22-23	-0.02715926614112618
1103	24-25	-0.053085348623973516
1103	26-27	-0.056137561484035814
1103	28-29	-0.04336271284589088
1103	30-31	-0.044222020119185856
1103	32-33	-0.0668274968419098
1103	34-35	-0.05239158543917455
1103	36-37	-0.04488686276189213
1103	38-39	-0.029150154483417623
1103	40-41	-0.010671808909826552
1103	42-43	-0.03522105926921171
1103	44-45	-0.01688008212727965
1103	46-47	0.002240625321270784
1103	48-49	-0.021953220956817177
1103	50-51	-0.05421081665492267
1103	52-53	-0.08546450103948189
1103	54-55	-0.09986276387645177
1103	56-57	-0.15840572621228688
1103	58-59	-0.1541231831507659
1103	60-61	-0.14500632545448866
1103	62-63	-0.17238628246004595
1103	64-65	-0.17929088021596584
1103	66-67	-0.2303582610650352
1103	68-69	-0.19470192908255513
1103	70-71	-0.22205066646714755
1103	72-73	-0.22234982050095908
1103	74-75	-0.21571093459064627
1103	76-77	0.013874615643040045
1103	78-79	-0.12461762929586939
1103	80-81	-0.15982982736017703
1103	82-83	-0.15859653405666307
1103	84-85	-0.1342092339249632
1103	86-87	-0.1495753009702625
1103	88-89	-0.11540675492183539
1103	90-91	-0.11800457050482294
1103	92-93	-0.08041096374582679
1103	94-95	-0.0810824504373997
1103	96-97	-0.11236827059811105
1103	98-99	-0.09655081814611322
1103	100	-0.1523869620249485
1104	1	0.056576071176500875
1104	2	-9.467748240012952E-4
1104	3	-0.028330644122682713
1104	4	-0.027701065038243655
1104	5	-0.06428263219451225
1104	6	-0.06595111313856705
1104	7	-0.04191781010970885
1104	8	-0.046509789487629405
1104	9	-0.05723117076878026
1104	10-11	-0.07657302836364721
1104	12-13	-0.07622937682474884
1104	14-15	-0.11831053138225656
1104	16-17	-0.07365378114750598
1104	18-19	-0.07872277791953763
1104	20-21	-0.07775176875158252
1104	22-23	-0.05891488369342568
1104	24-25	-0.06721360108020491
1104	26-27	-0.06543482261084677
1104	28-29	-0.0939296688700324
1104	30-31	-0.03620884827233084
1104	32-33	-0.06239753966371353
1104	34-35	-0.05281266846169075
1104	36-37	-0.06589595666756765
1104	38-39	-0.0359560398010359
1104	40-41	-0.05769686080382286
1104	42-43	-0.08318308859531243
1104	44-45	-0.0620135863498561
1104	46-47	-0.05092015518490456
1104	48-49	-0.0708984372552024
1104	50-51	-0.10965924247775405
1104	52-53	-0.09582612114245848
1104	54-55	-0.07352026797203592
1104	56-57	-0.1550720418416489
1104	58-59	-0.19239097938539373
1104	60-61	-0.15769195787459012
1104	62-63	-0.1443351151001977
1104	64-65	-0.16505069799507766
1104	66-67	-0.15611511118364518
1104	68-69	-0.18535799758125648
1104	70-71	-0.1296512726160799
1104	72-73	-0.1239210457532991
1104	74-75	-0.14593771961622792
1104	76-77	0.035696862252613926
1104	78-79	-0.08475755016519315
1104	80-81	-0.09847756447319966
1104	82-83	-0.06851694313171919
1104	84-85	-0.06480891501767871
1104	86-87	-0.037766940606680066
1104	88-89	-0.006699293371667636
1104	90-91	-0.054528046116203654
1104	92-93	-0.03519073594254962
1104	94-95	-0.09076981508167847
1104	96-97	-0.027431907584258397
1104	98-99	-0.07326529646557134
1104	100	-0.10032245760796954
1105	1	0.00842768801219762
1105	2	-0.05040723435836014
1105	3	-0.05841069153205325
1105	4	-0.04393452229887629
1105	5	-0.07778807237680496
1105	6	-0.08446513096114217
1105	7	-0.09062203841027383
1105	8	-0.06665158831409457
1105	9	-0.1216429967517314
1105	10-11	-0.12000476400307036
1105	12-13	-0.11790969214737146
1105	14-15	-0.1561941931831896
1105	16-17	-0.1568041696264899
1105	18-19	-0.12147865397471946
1105	20-21	-0.11822608541398694
1105	22-23	-0.113728157831396
1105	24-25	-0.11455418848636612
1105	26-27	-0.1029950831533526
1105	28-29	-0.11014767379569435
1105	30-31	-0.10071792895946885
1105	32-33	-0.13630094083405453
1105	34-35	-0.10266917286080712
1105	36-37	-0.10869241010796316
1105	38-39	-0.07119309392557938
1105	40-41	-0.07795293619375343
1105	42-43	-0.09021141846883296
1105	44-45	-0.09175478532409898
1105	46-47	-0.08146571731555952
1105	48-49	-0.08386458100544303
1105	50-51	-0.12977253289039226
1105	52-53	-0.10387134520874497
1105	54-55	-0.11042706337399721
1105	56-57	-0.129949636389604
1105	58-59	-0.14916520266302769
1105	60-61	-0.1279504081372238
1105	62-63	-0.1316897287547576
1105	64-65	-0.1937584587616925
1105	66-67	-0.16340066438039003
1105	68-69	-0.17244564907753812
1105	70-71	-0.14231508621799094
1105	72-73	-0.12409142143929586
1105	74-75	-0.12601015312107222
1105	76-77	0.048412252102231434
1105	78-79	-0.1262379122169648
1105	80-81	-0.10520761110450394
1105	82-83	-0.12525189088054134
1105	84-85	-0.09987943918939379
1105	86-87	-0.12056953243932611
1105	88-89	-0.05910805063493996
1105	90-91	-0.09961212930857499
1105	92-93	-0.09156738994263236
1105	94-95	-0.09572011774261213
1105	96-97	-0.0957609647274218
1105	98-99	-0.08405652266128527
1105	100	-0.07888013227217527
1106	1	-0.013898217897171605
1106	2	-0.031602693790077296
1106	3	-0.0628441489987921
1106	4	-0.039880715374707165
1106	5	-0.08667489962497399
1106	6	-0.08398566363145932
1106	7	-0.0744956787412221
1106	8	-0.0700139395454471
1106	9	-0.1326419061927595
1106	10-11	-0.11322659710725702
1106	12-13	-0.10779259057832036
1106	14-15	-0.16305147065806125
1106	16-17	-0.14084284371886469
1106	18-19	-0.14080047728322143
1106	20-21	-0.15743288353774432
1106	22-23	-0.15818212492576578
1106	24-25	-0.14326964342678394
1106	26-27	-0.12327135228051134
1106	28-29	-0.12143252328466758
1106	30-31	-0.1310613192184107
1106	32-33	-0.10803239653230179
1106	34-35	-0.08667389835895989
1106	36-37	-0.08409157215738361
1106	38-39	-0.09579035700166116
1106	40-41	-0.0963352008322289
1106	42-43	-0.1172648133896601
1106	44-45	-0.10935462991160705
1106	46-47	-0.153450307184265
1106	48-49	-0.1371305925707702
1106	50-51	-0.1578497102797698
1106	52-53	-0.1761845461873932
1106	54-55	-0.1648477113760194
1106	56-57	-0.20859352292627165
1106	58-59	-0.15178565583702408
1106	60-61	-0.14902804433857852
1106	62-63	-0.20401341091513103
1106	64-65	-0.20891010196236692
1106	66-67	-0.20451508843247268
1106	68-69	-0.19838914623088755
1106	70-71	-0.17878103577939441
1106	72-73	-0.16540614959001232
1106	74-75	-0.19082677209787846
1106	76-77	0.05678514923221911
1106	78-79	-0.1423964881352333
1106	80-81	-0.1848189311032229
1106	82-83	-0.15947930412651345
1106	84-85	-0.1456096972255665
1106	86-87	-0.13836205579384142
1106	88-89	-0.09571073443751033
1106	90-91	-0.12724338960319415
1106	92-93	-0.14832355826993293
1106	94-95	-0.15555837928844696
1106	96-97	-0.13023533813969124
1106	98-99	-0.128648369162093
1106	100	-0.10395589079424639
1107	1	-0.022379258771046295
1107	2	-0.06127709669269876
1107	3	-0.0892447708532842
1107	4	-0.053819325673259755
1107	5	-0.07509807404255042
1107	6	-0.09675731775528362
1107	7	-0.11229773907546559
1107	8	-0.11246436270068472
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2316	86-87	0.02799045756516705
2316	88-89	0.06033399552229213
2316	90-91	0.037364339946755365
2316	92-93	0.0926271349741441
2316	94-95	0.03588785177107923
2316	96-97	0.0791958713970331
2316	98-99	0.056905899937039095
2316	100	0.05170921107361082
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	43.0
8	72.0
9	54.0
10	68.0
11	105.0
12	289.0
13	1348.0
14	5715.0
15	17718.0
16	31055.0
17	40425.0
18	47490.0
19	53990.0
20	65112.0
21	86232.0
22	125607.0
23	195900.0
24	289094.0
25	362428.0
26	391587.0
27	360143.0
28	345564.0
29	389616.0
30	480302.0
31	480597.0
32	564138.0
33	711681.0
34	987920.0
35	1908871.0
36	2945679.0
37	6148236.0
38	9068814.0
39	2848100.0
40	5825.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	16.879630687547255	34.70934077441374	27.038050556808063	21.372977981230946
2	20.807789955873993	32.36614904028963	28.020811731597878	18.805249272238502
3	24.16052233912323	27.003185573293546	27.244734127895825	21.591557959687393
4	18.5877766700341	27.203598454241764	27.643066753849688	26.565558121874457
5	20.268628806408383	32.373451444593236	26.904966547953883	20.452953201044497
6	26.702472350492656	26.369750764926426	27.209526165511406	19.718250719069513
7	20.77672744480273	26.81703274928141	32.56526432441151	19.84097548150435
8	25.49463245343042	27.224500208668665	27.02785856221451	20.253008775686396
9	25.400446232086125	26.772229714979563	27.68471473128733	20.14260932164698
10-11	20.020852005766336	30.22830023297119	29.759730072758778	19.991117688503692
12-13	20.054128125964105	26.928160826218257	29.495443261868594	23.522267785949047
14-15	19.704826357055257	33.27502414173876	26.493688237338237	20.52646126386774
16-17	22.944516036178094	27.067309002150125	26.49514738341719	23.493027578254598
18-19	20.290150986124466	29.80442015227999	26.873146496330534	23.032282365265004
20-21	22.9649049738078	29.790800300286534	26.886908790598067	20.357385935307594
22-23	23.103867413559673	27.351697506157137	29.530873822654986	20.013561257628208
24-25	20.008165896429166	29.988042597194298	26.56783105047586	23.435960455900673
26-27	19.94066192792513	26.768870756871223	32.80735513613048	20.483112179073164
28-29	20.118251718324615	27.19543114075536	32.29764928385413	20.388667857065894
30-31	20.405887626928056	26.82284821292174	32.654747901888356	20.11651625826185
32-33	20.063561407295488	27.288657889056765	32.15953032592273	20.488250377725016
34-35	20.074902760824507	27.362422245958335	32.115175168659334	20.447499824557816
36-37	20.10133897739081	27.355524747418425	32.07664189724216	20.46649437794861
38-39	20.60574822983408	27.019897973694235	32.02906332169037	20.34529047478131
40-41	20.211043422730455	27.86815345052584	31.99439156761612	19.92641155912759
42-43	20.268537121285085	27.14807669594787	32.254242966336605	20.329143216430438
44-45	20.32692039756628	27.23154753808875	32.10478558734845	20.33674647699651
46-47	20.721981039715494	27.398862291758853	31.986436406470325	19.89272026205533
48-49	20.473798221700346	27.804667996444692	31.80192140715886	19.9196123746961
50-51	20.870432925122568	27.53508625159742	31.23959767039618	20.354883152883836
52-53	21.029793922790088	27.716409165776966	30.92695882363597	20.32683808779698
54-55	20.896765040581467	28.027254306080966	30.548951467325963	20.527029186011607
56-57	21.189129776009157	28.442363228672907	30.063563242104312	20.30494375321362
58-59	21.325234899008912	28.700772390770336	29.345883412378885	20.628109297841867
60-61	21.673960439169495	28.7921757371808	28.777832713489655	20.756031110160052
62-63	21.304496949350156	28.883503194627615	29.35024954649421	20.461750309528018
64-65	21.664350061105022	29.3947602086577	28.416874329336427	20.524015400900847
66-67	21.232657419132433	29.7181422583852	28.370557318339422	20.678643004142945
68-69	21.11576157604535	29.70847485493514	28.347389212483343	20.82837435653617
70-71	21.0217034367761	29.731120204465434	28.272691470749006	20.974484888009464
72-73	20.92545638498673	29.71468317106553	28.14765786762129	21.21220257632645
74-75	20.931862608383582	29.650893625403512	28.006810886643983	21.410432879568912
76-77	20.993349815047786	29.61008067876235	27.788645063623612	21.607924442566254
78-79	21.00296641258987	29.661827652457646	27.510883257999435	21.82432267695305
80-81	21.03004175498216	29.679427921071134	27.25725494153228	22.033275382414423
82-83	20.960983310075495	29.772094015804925	27.022297981976802	22.244624692142782
84-85	20.91786972581687	29.751616941990655	26.849301401875493	22.48121193031698
86-87	20.936668010236176	29.717569303184703	26.695112883321347	22.650649803257775
88-89	20.880338916237783	29.697104533620234	26.624330626876073	22.798225923265907
90-91	20.830345707587504	29.626013171827292	26.556114945495814	22.987526175089386
92-93	20.80900169264338	29.560769143506565	26.521489215377	23.108739948473055
94-95	20.74660078920894	29.52153258259903	26.536049046046767	23.195817582145263
96-97	20.711619329203664	29.430479135083786	26.520423178207142	23.337478357505407
98-99	20.67706207628021	29.366385516495225	26.532459405185232	23.42409300203933
100	20.654424284513738	29.364850810968175	26.49101920067493	23.489705703843157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	476319.0
1	277692.0
2	53087.5
3	22997.0
4	17860.0
5	16257.0
6	15739.0
7	15611.0
8	15321.0
9	15336.0
10	16459.5
11	27249.5
12	254424.5
13	283173.5
14	81376.0
15	68455.5
16	70805.5
17	75136.5
18	79922.0
19	86502.0
20	96549.5
21	110591.0
22	129041.5
23	151045.0
24	175675.0
25	203496.0
26	235361.0
27	272005.5
28	314910.5
29	363312.0
30	414708.5
31	467324.0
32	523223.0
33	580739.0
34	627930.0
35	678684.0
36	749640.0
37	798485.5
38	824503.5
39	852085.5
40	866351.5
41	867474.5
42	864072.0
43	872581.0
44	889480.5
45	904482.5
46	913487.0
47	918215.0
48	925952.5
49	935140.0
50	945659.0
51	949860.5
52	934066.0
53	893928.5
54	842404.5
55	788108.5
56	725097.5
57	656861.0
58	590084.5
59	513816.0
60	422283.5
61	339208.0
62	271494.0
63	206515.5
64	153620.0
65	115779.0
66	84730.0
67	62359.5
68	46974.0
69	35152.5
70	27547.0
71	23509.0
72	20740.0
73	16006.0
74	11037.5
75	7940.5
76	5731.5
77	3976.5
78	2652.0
79	1640.0
80	927.5
81	615.0
82	421.5
83	266.5
84	203.0
85	168.0
86	116.5
87	73.5
88	45.5
89	32.0
90	16.5
91	12.0
92	8.5
93	4.0
94	4.0
95	4.0
96	2.5
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4475028123450223
2	0.04313217714282597
3	0.0071789125194087885
4	1.7265301874480013E-4
5	1.726530187448001E-5
6	5.870202637323204E-4
7	2.417142262427202E-5
8	1.104979319966721E-4
9	0.0
10-11	4.834284524854404E-5
12-13	4.3163254686200034E-5
14-15	4.091876544251763E-4
16-17	2.796978903665762E-4
18-19	4.730692713607523E-4
20-21	4.989672241724724E-4
22-23	3.9537541292559227E-4
24-25	0.0012154772519633928
26-27	0.009297365059407486
28-29	0.012797041749364585
30-31	0.012436196940187954
32-33	0.012320519417628937
34-35	0.015227996253291371
36-37	0.019706615559531487
38-39	0.016693820382434724
40-41	0.02289206375537305
42-43	0.025153818300929928
44-45	0.021602345705349392
46-47	0.010997997294043767
48-49	0.018513583200004916
50-51	0.010231417890816856
52-53	0.016814677495556082
54-55	0.04887289001609057
56-57	0.04399889529692486
58-59	0.04200647946060987
60-61	0.06532844923265746
62-63	0.03765217032786601
64-65	0.02629678128502051
66-67	0.03245186140327263
68-69	0.028014678821531268
70-71	0.017593342610095132
72-73	0.009844675128828502
74-75	0.010158903622944039
76-77	0.009941360819325592
78-79	0.010207246468192583
80-81	0.008822569257859287
82-83	0.007588100173833966
84-85	0.00741199409471427
86-87	0.007355018598528486
88-89	0.007667520562456573
90-91	0.0075293981474607325
92-93	0.007434438987151093
94-95	0.00764507567001975
96-97	0.009007307987916222
98-99	0.008693079493800685
100	0.009540805815837654
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.8959818E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.302707166679546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.95412874886217	33.5032223847662
2	13.894793037160788	12.58943480208226
3	4.7363974167716645	6.437148755910725
4	2.2743629782872903	4.121391999843451
5	1.2425455901904108	2.814533950682259
6	0.7949683083674391	2.1608529888456416
7	0.5467568239165577	1.7338694999692907
8	0.38475506698178485	1.394435690429758
9	0.3025059833305543	1.233390598109329
>10	1.6787093969000098	13.728194576106928
>50	0.10890018714206594	3.3673150660644877
>100	0.07803084002441893	7.720280220736194
>500	0.002411457538221704	0.6452768920507365
>1k	6.044666018011132E-4	0.5386274658665232
>5k	6.866360727909267E-5	0.23765690950360263
>10k+	6.103431758123312E-5	7.774368199032611
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1445979	4.993052787831747	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	286505	0.9893190627095791	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	264749	0.9141942811933418	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACACGAATCTCGTATG	117763	0.406642748928878	TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACACACGAATCTCGTATG	72130	0.24906924484124865	TruSeq Adapter, Index 5 (97% over 36bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29421	0.10159248928981529	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.7193104597549612	0.0	0.0	0.0	0.0
2	0.7193553495398348	6.906120749792005E-6	0.0	3.4530603748960025E-6	3.4530603748960025E-6
3	0.7193622556605846	1.726530187448001E-5	0.0	3.4530603748960025E-6	6.906120749792005E-6
4	0.7193622556605846	2.4171422624272017E-5	0.0	3.4530603748960025E-6	1.0359181124688007E-5
5	0.7193622556605846	3.107754337406402E-5	0.0	3.4530603748960025E-6	2.0718362249376014E-5
6	0.7194865658340809	3.107754337406402E-5	0.0	6.906120749792005E-6	2.0718362249376014E-5
7	0.7194900188944557	3.107754337406402E-5	0.0	1.0359181124688007E-5	2.4171422624272017E-5
8	0.7195038311359554	3.107754337406402E-5	0.0	1.0359181124688007E-5	3.107754337406402E-5
9	0.7195625331623285	3.107754337406402E-5	0.0	1.381224149958401E-5	3.7983664123856026E-5
10-11	0.7195987902962651	3.107754337406402E-5	0.0	1.381224149958401E-5	4.143672449875203E-5
12-13	0.7196505862018885	3.9710194311304024E-5	0.0	1.8991832061928013E-5	4.661631506109603E-5
14-15	0.7198577698243822	4.143672449875203E-5	0.0	2.4171422624272017E-5	5.6975496185784045E-5
16-17	0.7200545942657512	4.143672449875203E-5	0.0	2.762448299916802E-5	6.215508674812804E-5
18-19	0.7203567370485546	4.661631506109603E-5	0.0	2.9351013186616022E-5	6.215508674812804E-5
20-21	0.7207831900048542	4.8342845248544035E-5	0.0	3.107754337406402E-5	6.733467731047205E-5
22-23	0.7214030143421482	5.006937543599204E-5	0.0	3.107754337406402E-5	8.287344899750407E-5
24-25	0.7234282342520246	5.179590562344004E-5	0.0	3.9710194311304024E-5	9.150609993474406E-5
26-27	0.7299148081662667	5.8702026373232043E-5	0.0	4.8342845248544035E-5	9.841222068453607E-5
28-29	0.7480589139061578	5.8702026373232043E-5	0.0	5.179590562344004E-5	1.0704487162177607E-4
30-31	0.7901724382383895	6.388161693557604E-5	0.0	5.697549618578404E-5	1.1395099237156808E-4
32-33	0.8704750837867834	6.560814712302405E-5	0.0	6.215508674812804E-5	1.1740405274646409E-4
34-35	1.0027980838829857	7.078773768536806E-5	0.0	7.078773768536806E-5	1.2603670368370408E-4
36-37	1.1976145706440557	7.942038862260806E-5	0.0	8.287344899750406E-5	1.398489451832881E-4
38-39	1.4591700818009286	8.287344899750406E-5	0.0	9.150609993474406E-5	1.450285357456321E-4
40-41	1.7891946006014265	8.805303955984807E-5	0.0	1.0531834143432807E-4	1.571142470577681E-4
42-43	2.1835738056088614	9.323263012219207E-5	0.0	1.1222446218412008E-4	1.5884077724521612E-4
44-45	2.6433315292243895	1.0186528105943208E-4	0.0	1.1913058293391208E-4	1.6229383762011212E-4
46-47	3.174855933141569	1.0359181124688007E-4	0.0	1.2258364330880808E-4	1.691999583699041E-4
48-49	3.7726531983039395	1.0359181124688007E-4	0.0	1.381224149958401E-4	1.7955913949459213E-4
50-51	4.427691845300961	1.0877140180922407E-4	0.0	1.467550659330801E-4	1.8301219986948812E-4
52-53	5.1354241936188965	1.1049793199667208E-4	0.0	1.6574689799500811E-4	1.9855097155652013E-4
54-55	5.891697938156931	1.1049793199667208E-4	0.0	1.691999583699041E-4	2.0373056211886415E-4
56-57	6.694777916076682	1.1395099237156808E-4	0.0	1.7955913949459213E-4	2.2272239418079216E-4
58-59	7.545016339536389	1.2085711312136008E-4	0.0	1.8301219986948812E-4	2.3308157530548018E-4
60-61	8.433264325072761	1.2603670368370408E-4	0.0	1.8646526024438415E-4	2.4171422624272017E-4
62-63	9.346353626946136	1.381224149958401E-4	0.0	1.8646526024438415E-4	2.5034687717996016E-4
64-65	10.284268706384825	1.381224149958401E-4	0.0	1.8819179043183214E-4	2.607060583046482E-4
66-67	11.244794425158336	1.467550659330801E-4	0.0	1.9164485080672814E-4	2.710652394293362E-4
68-69	12.220784674820816	1.502081263079761E-4	0.0	1.9855097155652016E-4	2.779713601791282E-4
70-71	13.215152111798492	1.519346564954241E-4	0.0	2.1063668286865615E-4	2.814244205540242E-4
72-73	14.216280640990217	1.588407772452161E-4	0.0	2.1408974324355214E-4	2.935101318661602E-4
74-75	15.228251779759113	1.6574689799500811E-4	0.0	2.1754280361844817E-4	2.935101318661602E-4
76-77	16.25077201797332	1.691999583699041E-4	0.0	2.2099586399334416E-4	2.935101318661602E-4
78-79	17.27670215330773	1.7783260930714413E-4	0.0	2.2444892436824016E-4	2.952366620536082E-4
80-81	18.30932086658832	1.8128566968204012E-4	0.0	2.2962851493058418E-4	3.073223733657442E-4
82-83	19.345817366669912	1.9164485080672814E-4	0.0	2.3308157530548018E-4	3.211346148653282E-4
84-85	20.384399515217947	1.9682444136907213E-4	0.0	2.4171422624272017E-4	3.366733865523602E-4
86-87	21.422156382336382	2.0200403193141616E-4	0.0	2.4862034699251216E-4	3.401264469272562E-4
88	22.199348766625537	2.0373056211886415E-4	0.0	2.4862034699251216E-4	3.418529771147042E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	195235	0.0	84.59645	1
GGTATCA	195220	0.0	83.57691	8
GTGGTAT	196110	0.0	83.32148	6
AGTGGTA	196820	0.0	83.13073	5
GTATCAA	197830	0.0	82.20589	9
TGGTATC	199880	0.0	81.580536	7
GCAGTGG	202065	0.0	81.40913	3
AGCAGTG	204885	0.0	80.735535	2
CAGTGGT	203815	0.0	80.47577	4
CAACGCA	192715	0.0	44.282356	12-13
ATCAACG	194005	0.0	44.049633	10-11
ACGCAGA	196170	0.0	43.52153	14-15
AGAGTAC	198015	0.0	42.7371	18-19
TCAACGC	192435	0.0	42.17248	12-13
AGTACTT	195480	0.0	41.75312	20-21
AACGCAG	194690	0.0	41.542767	14-15
GAGTACT	186200	0.0	41.44292	20-21
GCAGAGT	207700	0.0	41.067	16-17
CGCAGAG	197375	0.0	40.896626	16-17
TATCAAC	203440	0.0	39.99623	10-11
>>END_MODULE