FastQCFastQC Report
Thu 26 May 2016
SRR1660666_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660666_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27817902
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2131640.7662835249042145No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA648820.2332382938152561No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA365100.068.6818168
GTATCAA394750.063.4637349
GTGGTAT395900.063.4451146
TGGTATC400300.062.6188937
AGTGGTA409100.061.5356645
GCAGTGG501500.050.572313
AAGCAGT511150.049.8381841
CAGTGGT517500.048.9361724
AGCAGTG574700.044.4901922
CAACGCA330300.040.32487512-13
TCAACGC325700.038.4303612-13
ATCAACG348550.038.30772410-11
ACGCAGA369400.036.27003514-15
AACGCAG347750.035.95326614-15
AGAGTAC387250.034.186918-19
CGCAGAG383200.032.63334716-17
GAGTACT359550.032.55562620-21
TATCAAC406100.030.98367910-11
AGTACTT457450.027.46852720-21
GTACTTT428200.027.23213422-23
Produced by FastQC (version 0.11.5)