##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660666_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27817902 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.905883376826907 34.0 31.0 34.0 30.0 34.0 2 32.099928599935396 34.0 31.0 34.0 30.0 34.0 3 32.08553689634826 34.0 31.0 34.0 30.0 34.0 4 35.52473576188456 37.0 35.0 37.0 33.0 37.0 5 35.52311385668121 37.0 35.0 37.0 33.0 37.0 6 35.52783610352787 37.0 35.0 37.0 33.0 37.0 7 35.50279039734916 37.0 35.0 37.0 33.0 37.0 8 35.500768857407 37.0 35.0 37.0 33.0 37.0 9 37.12782092625102 39.0 37.0 39.0 33.0 39.0 10-11 37.12942953066698 39.0 37.0 39.0 33.5 39.0 12-13 37.12756343019686 39.0 37.0 39.0 33.0 39.0 14-15 38.527939418292576 40.0 38.0 41.0 34.0 41.0 16-17 38.495211536801015 40.0 38.0 41.0 34.0 41.0 18-19 38.46231437582892 40.0 38.0 41.0 33.5 41.0 20-21 38.41059068724881 40.0 38.0 41.0 34.0 41.0 22-23 38.37132536091327 40.0 38.0 41.0 34.0 41.0 24-25 38.360644325370046 40.0 38.0 41.0 34.0 41.0 26-27 38.29749351694459 40.0 38.0 41.0 33.0 41.0 28-29 38.21712138823409 40.0 38.0 41.0 33.0 41.0 30-31 38.113026855871446 40.0 38.0 41.0 33.0 41.0 32-33 38.01519365119627 40.0 38.0 41.0 33.0 41.0 34-35 37.90823229587911 40.0 38.0 41.0 32.5 41.0 36-37 37.79977790920394 40.0 38.0 41.0 32.0 41.0 38-39 37.68291039705295 40.0 37.0 41.0 32.0 41.0 40-41 37.55498725245347 40.0 37.0 41.0 31.5 41.0 42-43 37.404205464524246 40.0 37.0 41.0 31.0 41.0 44-45 37.43180529933566 40.0 37.0 41.0 31.0 41.0 46-47 37.479452871751434 40.0 37.0 41.0 31.0 41.0 48-49 37.41042554179679 40.0 37.0 41.0 31.0 41.0 50-51 36.76330326780215 39.0 35.5 40.5 30.5 41.0 52-53 36.73374712082888 39.0 36.0 40.0 30.0 41.0 54-55 36.8826620893265 39.0 36.0 41.0 30.0 41.0 56-57 36.68889010752859 39.0 35.0 41.0 30.0 41.0 58-59 36.46517165456978 39.0 35.0 41.0 29.5 41.0 60-61 36.203344989855815 38.5 35.0 40.5 29.0 41.0 62-63 35.91983764627541 38.0 35.0 40.0 29.0 41.0 64-65 35.61357526171456 37.5 34.0 40.0 28.0 41.0 66-67 35.29934450124959 37.0 34.0 40.0 28.0 41.0 68-69 35.04392466405267 36.5 34.0 39.0 28.0 41.0 70-71 34.72064990738698 36.0 34.0 39.0 28.0 41.0 72-73 34.45002716236473 35.5 34.0 39.0 28.0 40.5 74-75 34.141254613665694 35.0 34.0 37.5 27.5 40.0 76-77 33.81569113299774 35.0 33.0 37.0 27.0 39.0 78-79 33.482267893531294 35.0 33.0 37.0 26.5 39.0 80-81 33.16606390733564 35.0 33.0 36.0 26.0 38.5 82-83 32.78609943337927 35.0 33.0 36.0 26.0 37.0 84-85 32.42747387994968 35.0 32.5 35.0 25.0 37.0 86-87 32.20609239330845 35.0 32.0 35.0 25.0 36.5 88-89 32.0253032202069 35.0 32.0 35.0 25.0 36.0 90-91 31.870890227451373 35.0 32.0 35.0 24.5 36.0 92-93 31.755609894664232 35.0 32.0 35.0 24.0 36.0 94-95 31.641948860845076 35.0 32.0 35.0 24.0 35.0 96-97 31.554971093794205 35.0 32.0 35.0 24.0 35.0 98-99 31.43894539566643 35.0 31.0 35.0 23.5 35.0 100 31.312144711703997 35.0 31.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.21664339550467204 1101 2 -0.18882043655496972 1101 3 -0.18837996335513907 1101 4 -0.15129678401788738 1101 5 -0.08809085672545791 1101 6 -0.13002441658660047 1101 7 -0.12530602443798244 1101 8 -0.12486529062239526 1101 9 -0.16929822051334753 1101 10-11 -0.2082182436462361 1101 12-13 -0.18136139987169742 1101 14-15 -0.24978491241245138 1101 16-17 -0.26684451849376245 1101 18-19 -0.2274517856862417 1101 20-21 -0.24461752448510055 1101 22-23 -0.28730826035472035 1101 24-25 -0.24886805963144099 1101 26-27 -0.2635169541579998 1101 28-29 -0.29513324868062796 1101 30-31 -0.2561226349142487 1101 32-33 -0.26290439228662876 1101 34-35 -0.2839359469424636 1101 36-37 -0.27730436549228443 1101 38-39 -0.2718332654444353 1101 40-41 -0.29129011514634584 1101 42-43 -0.27934755620692897 1101 44-45 -0.24538172795762137 1101 46-47 -0.2832100349142266 1101 48-49 -0.33369017825054215 1101 50-51 -0.12865816726024804 1101 52-53 -0.14982309035998043 1101 54-55 -0.2921521501290769 1101 56-57 -0.29204491427440615 1101 58-59 -0.32330642102746054 1101 60-61 -0.33777438993570286 1101 62-63 -0.33326707425570135 1101 64-65 -0.3408624935908975 1101 66-67 -0.3656369891028959 1101 68-69 -0.41216669381163484 1101 70-71 -0.40665472473617825 1101 72-73 -0.3891986119765818 1101 74-75 -0.3501357912447247 1101 76-77 -0.33143102016740755 1101 78-79 -0.31531735908959746 1101 80-81 -0.3485805106792128 1101 82-83 -0.40176165819581655 1101 84-85 -0.45824908714919843 1101 86-87 -0.44577071517868205 1101 88-89 -0.4312390093934724 1101 90-91 -0.46959999006292463 1101 92-93 -0.4722806504869119 1101 94-95 -0.4769796527563521 1101 96-97 -0.4671936006246291 1101 98-99 -0.5077380341900977 1101 100 -0.4834680324074796 1102 1 -0.2700554873133285 1102 2 -0.18764833232948064 1102 3 -0.2116165549215907 1102 4 -0.16226447876680794 1102 5 -0.18511710011920712 1102 6 -0.1685290285704113 1102 7 -0.18310513119812555 1102 8 -0.16781634380737387 1102 9 -0.20668374575495108 1102 10-11 -0.1899309888006755 1102 12-13 -0.20791057480298036 1102 14-15 -0.2810332027071496 1102 16-17 -0.29798357757854177 1102 18-19 -0.2885166853046073 1102 20-21 -0.35082380208722697 1102 22-23 -0.35236942196376475 1102 24-25 -0.32555794144391825 1102 26-27 -0.32129426514315895 1102 28-29 -0.32454030201590456 1102 30-31 -0.3138482145106636 1102 32-33 -0.3400322761107688 1102 34-35 -0.36257419955914827 1102 36-37 -0.3379172707161118 1102 38-39 -0.31925112570721126 1102 40-41 -0.27843007881683235 1102 42-43 -0.29536007883880444 1102 44-45 -0.34182260027180433 1102 46-47 -0.3664776661821776 1102 48-49 -0.36525759092474885 1102 50-51 -0.13631426356482734 1102 52-53 -0.13212857586137972 1102 54-55 -0.304283299168155 1102 56-57 -0.2933742611922341 1102 58-59 -0.3402078167677871 1102 60-61 -0.35465775321984694 1102 62-63 -0.27488274293309445 1102 64-65 -0.30164261498020295 1102 66-67 -0.2998248269747137 1102 68-69 -0.37999641211775526 1102 70-71 -0.3700954853826204 1102 72-73 -0.38702255902847327 1102 74-75 -0.32206730997791055 1102 76-77 -0.30554653633134876 1102 78-79 -0.32668604942980295 1102 80-81 -0.31500441099540666 1102 82-83 -0.3452513483899722 1102 84-85 -0.43529017532973313 1102 86-87 -0.4266022793723323 1102 88-89 -0.39591626581675143 1102 90-91 -0.3792281842591194 1102 92-93 -0.3633173009575792 1102 94-95 -0.4093047970537178 1102 96-97 -0.4322376734561324 1102 98-99 -0.4388607072367492 1102 100 -0.38928070973318896 1103 1 -0.23659645560898213 1103 2 -0.2222196737461175 1103 3 -0.22162493546841233 1103 4 -0.17296533696923433 1103 5 -0.17463100262332176 1103 6 -0.2003781378436429 1103 7 -0.15788471235896395 1103 8 -0.15780787040876731 1103 9 -0.18541648236958252 1103 10-11 -0.2160630309728333 1103 12-13 -0.2389829998690871 1103 14-15 -0.29110427368114244 1103 16-17 -0.2780883494521973 1103 18-19 -0.26110979546799484 1103 20-21 -0.2791369595297226 1103 22-23 -0.25575480571129106 1103 24-25 -0.2536714912263349 1103 26-27 -0.2584489621156578 1103 28-29 -0.32418057692213154 1103 30-31 -0.31161206781060713 1103 32-33 -0.2745064687121044 1103 34-35 -0.30098295176943424 1103 36-37 -0.2872328354812055 1103 38-39 -0.28208946903860976 1103 40-41 -0.282326247855778 1103 42-43 -0.31742527856530955 1103 44-45 -0.33437781004975164 1103 46-47 -0.3335733528623166 1103 48-49 -0.3346335061429073 1103 50-51 -0.06214424214511638 1103 52-53 -0.1441271815262155 1103 54-55 -0.26521975401667675 1103 56-57 -0.2816823495101133 1103 58-59 -0.33644265612932855 1103 60-61 -0.32025231972881585 1103 62-63 -0.3124367648272681 1103 64-65 -0.32208338372974055 1103 66-67 -0.2841189255369301 1103 68-69 -0.2984241001924559 1103 70-71 -0.30343860401918477 1103 72-73 -0.29670119522909033 1103 74-75 -0.27604598037274286 1103 76-77 -0.2599457788661894 1103 78-79 -0.2572226419815209 1103 80-81 -0.26354381043096 1103 82-83 -0.3450458628480604 1103 84-85 -0.38092787014350904 1103 86-87 -0.3838243635063492 1103 88-89 -0.3776135695292169 1103 90-91 -0.41030969150379093 1103 92-93 -0.4066016868739908 1103 94-95 -0.4264459994946179 1103 96-97 -0.5181535197609328 1103 98-99 -0.5490935500446845 1103 100 -0.5213130031892099 1104 1 -0.34997458649814916 1104 2 -0.3113245961684967 1104 3 -0.28633910677562113 1104 4 -0.2267564516090843 1104 5 -0.2667767858236161 1104 6 -0.2710270816763014 1104 7 -0.2264634387876754 1104 8 -0.257287828524035 1104 9 -0.2724221760066001 1104 10-11 -0.2802202206117457 1104 12-13 -0.2876144715355622 1104 14-15 -0.4103655792641234 1104 16-17 -0.39177868332827614 1104 18-19 -0.3988226464646303 1104 20-21 -0.37732885516047077 1104 22-23 -0.40563466540510973 1104 24-25 -0.44149251383541355 1104 26-27 -0.39436278476290454 1104 28-29 -0.4242503863962881 1104 30-31 -0.40275018779522753 1104 32-33 -0.41821845175844885 1104 34-35 -0.4004916585202096 1104 36-37 -0.41965292125755127 1104 38-39 -0.37615427435629556 1104 40-41 -0.38291584810715307 1104 42-43 -0.4427708573372868 1104 44-45 -0.43363745835845435 1104 46-47 -0.44176255871549586 1104 48-49 -0.4696726996516176 1104 50-51 -0.3423333627416625 1104 52-53 -0.3353607454641718 1104 54-55 -0.4194719535315201 1104 56-57 -0.4001648797209896 1104 58-59 -0.4594205873555737 1104 60-61 -0.4515908498495804 1104 62-63 -0.47589071343145406 1104 64-65 -0.4856740131127921 1104 66-67 -0.4733244710050002 1104 68-69 -0.4977931682448897 1104 70-71 -0.5278888515667504 1104 72-73 -0.45404914296764787 1104 74-75 -0.4046839092052963 1104 76-77 -0.41701188928693256 1104 78-79 -0.42326821844557116 1104 80-81 -0.39778750364371973 1104 82-83 -0.4833627526014084 1104 84-85 -0.5082167044944228 1104 86-87 -0.4919535618951514 1104 88-89 -0.4925542495069024 1104 90-91 -0.47154579459008517 1104 92-93 -0.5126284418075784 1104 94-95 -0.4884929109124805 1104 96-97 -0.4473492710487079 1104 98-99 -0.5104159887943105 1104 100 -0.47038386167908186 1105 1 -0.3928988893683538 1105 2 -0.31035057540384514 1105 3 -0.3132442837168057 1105 4 -0.26932343569967543 1105 5 -0.2609740616998053 1105 6 -0.2608143932497313 1105 7 -0.2404431245286034 1105 8 -0.25027006116030037 1105 9 -0.2956492591494708 1105 10-11 -0.30754407467895106 1105 12-13 -0.30231492227714796 1105 14-15 -0.40361313023712597 1105 16-17 -0.39899803002933965 1105 18-19 -0.3741585644246612 1105 20-21 -0.41578202972884526 1105 22-23 -0.3915149355008083 1105 24-25 -0.40152025001140856 1105 26-27 -0.3846983444637928 1105 28-29 -0.3944855192101997 1105 30-31 -0.35711542002346164 1105 32-33 -0.40031993291249535 1105 34-35 -0.389680344040471 1105 36-37 -0.38951110824822877 1105 38-39 -0.35971776246836384 1105 40-41 -0.318030551863437 1105 42-43 -0.3645147378469744 1105 44-45 -0.35916273459018555 1105 46-47 -0.4057095297291653 1105 48-49 -0.41156407129080463 1105 50-51 -0.19321312301173776 1105 52-53 -0.2546722884059207 1105 54-55 -0.4164729895861612 1105 56-57 -0.39292288401782827 1105 58-59 -0.4181940950612031 1105 60-61 -0.42960192057456226 1105 62-63 -0.42798137534678204 1105 64-65 -0.44521787902156973 1105 66-67 -0.4210828496954875 1105 68-69 -0.47768456247238333 1105 70-71 -0.4892935985032594 1105 72-73 -0.39707401223117245 1105 74-75 -0.3973820757097286 1105 76-77 -0.3987043813462563 1105 78-79 -0.3993622242432764 1105 80-81 -0.40110301050511765 1105 82-83 -0.43001008261820317 1105 84-85 -0.4784787175411793 1105 86-87 -0.45901011919418977 1105 88-89 -0.46563531424117954 1105 90-91 -0.5030558038730426 1105 92-93 -0.5107893472383225 1105 94-95 -0.517862586442309 1105 96-97 -0.49183572190628055 1105 98-99 -0.5146675645009786 1105 100 -0.48958862481690346 1106 1 -0.413411965004034 1106 2 -0.39164053289664835 1106 3 -0.41703455019212754 1106 4 -0.32728040690047067 1106 5 -0.3319209015277593 1106 6 -0.3240273171284116 1106 7 -0.3040146986420069 1106 8 -0.31135568841620653 1106 9 -0.41429616864535035 1106 10-11 -0.41254769407647274 1106 12-13 -0.4273568087843529 1106 14-15 -0.5258471890971279 1106 16-17 -0.5275167274622348 1106 18-19 -0.4827050432526647 1106 20-21 -0.5067388056575481 1106 22-23 -0.5119084721923812 1106 24-25 -0.5115343119684326 1106 26-27 -0.5016502549163633 1106 28-29 -0.5187146296406411 1106 30-31 -0.4449305830646395 1106 32-33 -0.45649272066047786 1106 34-35 -0.46756617629310426 1106 36-37 -0.4921223136954609 1106 38-39 -0.4689018807518579 1106 40-41 -0.49484117156369223 1106 42-43 -0.47068112656099714 1106 44-45 -0.44298200721530634 1106 46-47 -0.5451712713226584 1106 48-49 -0.5537944339483829 1106 50-51 -0.26912405812509377 1106 52-53 -0.31002092635355183 1106 54-55 -0.5043995508703389 1106 56-57 -0.5676417860127998 1106 58-59 -0.5712486087150097 1106 60-61 -0.585448931410852 1106 62-63 -0.5679573485464431 1106 64-65 -0.5752644896797747 1106 66-67 -0.5466642088895526 1106 68-69 -0.5219296783434757 1106 70-71 -0.5999024269037037 1106 72-73 -0.502382543586549 1106 74-75 -0.4710009286118293 1106 76-77 -0.4494415840793806 1106 78-79 -0.4692351847282694 1106 80-81 -0.45994956887083305 1106 82-83 -0.509164273711626 1106 84-85 -0.527730324591495 1106 86-87 -0.5678502273914106 1106 88-89 -0.5344690720234908 1106 90-91 -0.5415761701476001 1106 92-93 -0.5257600837869418 1106 94-95 -0.5692930935252676 1106 96-97 -0.5299730212036771 1106 98-99 -0.582145786063645 1106 100 -0.5493931883609093 1107 1 -0.4240769125957513 1107 2 -0.39525357853328913 1107 3 -0.4054613435938492 1107 4 -0.32738608547379044 1107 5 -0.35717428391975403 1107 6 -0.3396455128645144 1107 7 -0.29020420597762353 1107 8 -0.31900311471897425 1107 9 -0.3943091280875919 1107 10-11 -0.42298222704386745 1107 12-13 -0.42081336576021044 1107 14-15 -0.5502001222184916 1107 16-17 -0.5467622395038063 1107 18-19 -0.5285723225006862 1107 20-21 -0.5323096091997996 1107 22-23 -0.5184579448249025 1107 24-25 -0.507264225844871 1107 26-27 -0.4997071305607932 1107 28-29 -0.5285976849434917 1107 30-31 -0.4556026081153064 1107 32-33 -0.5078428816882621 1107 34-35 -0.5179132161582416 1107 36-37 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2315 50-51 0.02070912092495547 2315 52-53 0.18163256681206263 2315 54-55 0.557442313378175 2315 56-57 0.5995494489316684 2315 58-59 0.596646738604214 2315 60-61 0.6029680579789911 2315 62-63 0.6285544643479284 2315 64-65 0.6549877636804453 2315 66-67 0.5296613658594467 2315 68-69 0.6163370538391533 2315 70-71 0.6705830518718727 2315 72-73 0.5923241463435573 2315 74-75 0.6234272250913904 2315 76-77 0.6278405868447408 2315 78-79 0.5515070048454049 2315 80-81 0.6163249591499351 2315 82-83 0.7206702587479086 2315 84-85 0.8165044444244032 2315 86-87 0.8355844754478596 2315 88-89 0.8204305516163757 2315 90-91 0.8197604439056789 2315 92-93 0.8591034182593589 2315 94-95 0.8903615088265475 2315 96-97 0.819586050302771 2315 98-99 0.9229351642252972 2315 100 0.9729269128716673 2316 1 0.4335964803607908 2316 2 0.38102068737865125 2316 3 0.4395601665386195 2316 4 0.42091243641701226 2316 5 0.37433768408097734 2316 6 0.43186162388951743 2316 7 -0.08981453877714074 2316 8 -0.5074397367363375 2316 9 0.11224399918177852 2316 10-11 0.29525513406296966 2316 12-13 0.356367205551507 2316 14-15 0.5078730519773913 2316 16-17 0.13808223882255533 2316 18-19 0.28441443535999866 2316 20-21 0.3921896024250131 2316 22-23 0.4882660764700901 2316 24-25 0.495129096610043 2316 26-27 0.5140910020480831 2316 28-29 0.47059270829099376 2316 30-31 0.5464188934495127 2316 32-33 0.5940873888772131 2316 34-35 0.5506926091885873 2316 36-37 0.5634877198108228 2316 38-39 0.48212151669223857 2316 40-41 0.5763047701048052 2316 42-43 0.540561873437376 2316 44-45 0.4874253098749506 2316 46-47 0.5170617237729473 2316 48-49 0.4503898625536067 2316 50-51 -0.08819325553751156 2316 52-53 0.06416908021105172 2316 54-55 0.400250594605545 2316 56-57 0.23008468691246975 2316 58-59 0.24848109783417982 2316 60-61 0.1812764498539181 2316 62-63 0.22474199764766922 2316 64-65 0.20821117323461635 2316 66-67 0.252857996266755 2316 68-69 0.47105858505882026 2316 70-71 0.49180153172647323 2316 72-73 0.42294641380332365 2316 74-75 0.31031680648824533 2316 76-77 0.31386172302526916 2316 78-79 0.42276569694841015 2316 80-81 0.4499060610214016 2316 82-83 0.5106704153302601 2316 84-85 0.6667098971030825 2316 86-87 0.5337603312105017 2316 88-89 0.6266154696640811 2316 90-91 0.6033253037011903 2316 92-93 0.6519205120719747 2316 94-95 0.7123943208803105 2316 96-97 0.5325917696884197 2316 98-99 0.6448046329613 2316 100 0.7384768621651325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 4.0 6 298.0 7 4141.0 8 11309.0 9 18241.0 10 22490.0 11 24903.0 12 27651.0 13 31743.0 14 38872.0 15 50740.0 16 64748.0 17 73838.0 18 77994.0 19 87484.0 20 98875.0 21 117000.0 22 141224.0 23 164763.0 24 203943.0 25 244673.0 26 275679.0 27 291014.0 28 330420.0 29 398652.0 30 484215.0 31 595008.0 32 746886.0 33 965283.0 34 1318796.0 35 1976960.0 36 3249004.0 37 6194903.0 38 7402022.0 39 2080036.0 40 4090.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.341073287830074 30.658858301620207 27.064928226261113 23.935140184288603 2 22.35537174336717 27.775221058369677 29.043946232059035 20.825460966204115 3 20.751109272160008 27.624710661774355 27.46755223905278 24.15662782701286 4 20.42648501936787 28.30486670401936 27.55627905926719 23.71236921734558 5 22.280437673241522 28.42335964112684 27.386082671923837 21.9101200137078 6 22.95819028541713 27.444310260819748 27.587967503453388 22.009531950309736 7 22.123615125009245 27.98091049103727 27.90015581152396 21.995318572429532 8 22.211341436584846 27.430479364892207 27.795625365775102 22.562553832747852 9 22.39073464281606 27.04801995858737 28.138347664787762 22.42289773380881 10-11 21.929460299366255 28.18034339593849 27.719888462689852 22.170307842005407 12-13 21.987298438558877 27.801303947696287 27.54714653978011 22.664251073964728 14-15 21.98422388677023 28.72030857197889 27.11359692689095 22.181870614359926 16-17 22.541014973082675 27.67668544915024 27.125244696834756 22.657054880932332 18-19 22.085589179630762 28.088401794048252 27.0903820198303 22.73562700649069 20-21 22.605128253865196 28.05447981805574 27.09185090100058 22.24854102707849 22-23 22.634687343921723 27.622433568370408 27.516405606196788 22.226473481511082 24-25 22.2537624409693 28.00804232693797 26.926977683064557 22.81121754902817 26-27 22.277533055816782 27.577631501959676 27.86703250334817 22.277802938875375 28-29 22.34091263684937 27.639837529024565 27.79766925304633 22.221580581079735 30-31 22.372396303772398 27.602157988850163 27.75607901610435 22.269366691273092 32-33 22.40091409554208 27.63486629586075 27.700661270709954 22.26355833788721 34-35 22.421478188696074 27.720499970217894 27.65483362129298 22.203188219793052 36-37 22.426286821734013 27.69342679242333 27.626663275001306 22.253623110841346 38-39 22.489607478708116 27.687411581763993 27.628583919112486 22.194397020415405 40-41 22.552775399328528 27.776098202244953 27.565067121918585 22.10605927650794 42-43 22.623599062692072 27.80100024802376 27.464646004565196 22.110754684718962 44-45 22.665427633475012 27.827695566905508 27.43667088775049 22.07020591186899 46-47 22.729309705635007 27.90396848291643 27.360135787314476 22.006586024134087 48-49 22.86336845762603 27.86101784087111 27.245606512596943 22.030007188905916 50-51 22.950384652571707 27.87625560025029 27.216523023171796 21.95683672400621 52-53 23.094112802663822 27.928251528296872 27.105177123393087 21.87245854564622 54-55 23.298007731239423 27.929667295271088 26.90235374970828 21.869971223781203 56-57 23.50346976435513 28.029674277511408 26.714938627071245 21.75191733106222 58-59 23.6973927686639 28.14040135421726 26.53152236554447 21.63068351157437 60-61 23.906614361290696 28.17053586454309 26.368850256849008 21.55399951731721 62-63 24.06430132830415 28.218530825703525 26.306016246103383 21.411151599888946 64-65 24.267621387594804 28.28954316372777 26.207078332244198 21.235757116433227 66-67 24.46105310274596 28.25700107587866 26.12793338720217 21.154012434173215 68-69 24.609701096430495 28.244107679766522 26.12230720497034 21.023884018832646 70-71 24.792376681170982 28.26179281341958 26.07556967667426 20.870260828735177 72-73 24.985781469116965 28.207641783982957 26.008767869338 20.797808877562083 74-75 25.164662107801895 28.19187211473807 25.98602355999696 20.657442217463082 76-77 25.38280253151177 28.18900123354214 25.888531437707563 20.539664797238533 78-79 25.616467120372416 28.12022560641309 25.77526611041091 20.488041162803576 80-81 25.801156736044224 28.078171182122112 25.71221562014304 20.408456461690623 82-83 25.9765758159402 28.052007297131848 25.63738768044579 20.33402920648216 84-85 26.146895710038777 27.967002232993277 25.56526515769808 20.32083689926986 86-87 26.285685168493156 27.874934526893746 25.56685106248776 20.272529242125337 88-89 26.376779240176706 27.870792091643455 25.53817410917923 20.214254559000608 90-91 26.470742681824166 27.82038674991704 25.50562671015541 20.203243858103384 92-93 26.510761415638832 27.847531430674046 25.52750946081485 20.114197692872267 94-95 26.55898263943482 27.953586774087295 25.47538040933223 20.012050177145657 96-97 26.575044719505286 28.04387034476134 25.393001711140613 19.988083224592756 98-99 26.58518163954341 28.185572657893793 25.339953585529578 19.88929211703322 100 26.59438994997036 28.36362652781232 25.232289514604812 19.809694007612514 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 72155.0 1 44201.0 2 13286.0 3 9407.0 4 7861.5 5 6875.0 6 6320.0 7 5991.5 8 5800.0 9 5803.5 10 5870.0 11 6819.5 12 28067.0 13 31470.0 14 14174.5 15 14598.5 16 16577.0 17 19385.5 18 23170.0 19 27526.5 20 34414.0 21 46310.5 22 63295.0 23 83691.5 24 106889.0 25 135368.0 26 167776.5 27 202956.0 28 246238.5 29 292287.5 30 335332.0 31 380641.0 32 429799.5 33 479362.0 34 525479.0 35 580379.5 36 645720.5 37 697864.5 38 742684.5 39 784563.5 40 816502.5 41 852415.5 42 894153.0 43 925058.0 44 953199.0 45 993035.5 46 1032446.5 47 1061606.5 48 1077468.0 49 1082715.5 50 1080387.5 51 1070663.5 52 1051108.0 53 1017058.5 54 964709.5 55 901687.5 56 832527.5 57 751343.0 58 659684.0 59 561483.0 60 461144.5 61 368364.5 62 286938.5 63 214523.0 64 156493.0 65 113929.5 66 82148.5 67 59436.5 68 43258.5 69 32253.5 70 24988.5 71 19486.5 72 15481.5 73 12039.0 74 9274.0 75 7652.5 76 5936.0 77 3907.5 78 2562.5 79 1919.5 80 1397.5 81 1029.0 82 824.5 83 621.5 84 491.0 85 387.5 86 313.5 87 259.5 88 211.0 89 196.5 90 164.0 91 121.0 92 101.0 93 95.5 94 74.5 95 53.0 96 45.0 97 41.5 98 37.5 99 31.5 100 48.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0019951181077566523 2 4.88893806585414E-4 3 4.026184289526938E-4 4 6.614445618508541E-4 5 5.859536064222241E-4 6 0.0010784422204090014 7 0.001146743561034905 8 9.418395391571945E-4 9 0.001085631835211728 10-11 0.0010946188537151365 12-13 0.0010730500093069563 14-15 0.0012240319201642165 16-17 0.001139553946232178 18-19 0.001319294316300345 20-21 0.14122200876255875 22-23 0.11274753933635973 24-25 0.1075728140820972 26-27 0.10097095028949343 28-29 0.1195165616731269 30-31 0.09482203222946145 32-33 0.08895710395413717 34-35 0.08759287454531978 36-37 0.08728012630140115 38-39 0.08323776537856808 40-41 0.08333123037100353 42-43 0.04349177734539434 44-45 0.026993408776837303 46-47 0.009483101924796485 48-49 0.002640386036301372 50-51 0.004229290907703968 52-53 0.005909863367841327 54-55 0.0073747473838968874 56-57 0.006801375603379435 58-59 0.009245844636306505 60-61 0.009105647147653335 62-63 0.010513014245287082 64-65 0.010793409222593421 66-67 0.012319404964472161 68-69 0.015396560100039175 70-71 0.0135506264994391 72-73 0.01382922407304476 74-75 0.012921535204200519 76-77 0.013272028925833443 78-79 0.01142609532523337 80-81 0.012337379001478976 82-83 0.2930666733961461 84-85 0.5218977333373308 86-87 0.4913706288849533 88-89 0.46932547249609263 90-91 0.46336348442093156 92-93 0.45739430673096776 94-95 0.4625888034259377 96-97 0.452395727039372 98-99 0.4533303769637264 100 0.4562493605736335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.7817902E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.9043387942109 #Duplication Level Percentage of deduplicated Percentage of total 1 77.59583408043298 38.72368792969304 2 10.723563627283728 10.703047046744887 3 4.016064293013316 6.012570992936088 4 2.105410524344173 4.202764804310752 5 1.2526381480540087 3.125603926352009 6 0.8455598555330429 2.5318263300783 7 0.5943698374133323 2.076314361473449 8 0.4274326353588145 1.7064594437318952 9 0.3369081662868297 1.5131861345622863 >10 1.8703874164770966 16.854738145290526 >50 0.15458264216153564 5.409084149286474 >100 0.07702371598271184 5.974984618351548 >500 1.5328675597572954E-4 0.045787039790583656 >1k 4.317933937207738E-5 0.04072023268187556 >5k 7.1478907839355436E-6 0.018930640210747654 >10k+ 2.1443672297644538E-5 1.0602942045054669 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 213164 0.7662835249042145 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64882 0.2332382938152561 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.011097170448008625 7.189614802726676E-6 0.0 0.0 7.189614802726676E-6 2 0.01110436006281135 2.1568844408180027E-5 0.0 3.594807401363338E-6 7.189614802726676E-6 3 0.011107954870212714 2.8758459210906705E-5 0.0 3.594807401363338E-6 1.0784422204090013E-5 4 0.011111549677614078 3.235326661227004E-5 0.0 3.594807401363338E-6 2.1568844408180027E-5 5 0.011111549677614078 4.3137688816360054E-5 0.0 3.594807401363338E-6 2.1568844408180027E-5 6 0.011111549677614078 4.3137688816360054E-5 0.0 7.189614802726676E-6 2.1568844408180027E-5 7 0.011111549677614078 5.3922111020450066E-5 0.0 7.189614802726676E-6 2.5163651809543364E-5 8 0.011111549677614078 5.751691842181341E-5 0.0 7.189614802726676E-6 2.5163651809543364E-5 9 0.011118739292416803 6.830134062590341E-5 0.0 7.189614802726676E-6 2.5163651809543364E-5 10-11 0.011129523714620894 7.369355172794843E-5 0.0 1.0784422204090013E-5 3.235326661227004E-5 12-13 0.01113491592572294 8.987018503408345E-5 0.0 1.0784422204090013E-5 4.134028511567839E-5 14-15 0.011149295155328393 1.1143902944226348E-4 0.0 1.2581825904771684E-5 5.751691842181341E-5 16-17 0.01116906659603589 1.2042604794567182E-4 0.0 1.4379229605453352E-5 6.830134062590343E-5 18-19 0.011187040633042707 1.2941306644908017E-4 0.0 1.4379229605453352E-5 7.189614802726676E-5 20-21 0.011249949762566566 1.4918450715657852E-4 0.0 1.797403700681669E-5 7.189614802726676E-5 22-23 0.011445866765940869 1.6895594786407687E-4 0.0 1.797403700681669E-5 7.728835912931176E-5 24-25 0.012709441567520081 1.8692998487089356E-4 3.594807401363338E-6 2.1568844408180027E-5 8.627537763272011E-5 26-27 0.018164561799088947 2.0490402187771026E-4 3.594807401363338E-6 2.1568844408180027E-5 8.807278133340178E-5 28-29 0.034168644349958525 2.138910403811186E-4 3.594807401363338E-6 2.5163651809543368E-5 9.166758873476512E-5 30-31 0.0723041586673215 2.3366248108861696E-4 3.594807401363338E-6 3.235326661227004E-5 1.0245201093885513E-4 32-33 0.14491747077116024 2.462443069933887E-4 3.594807401363338E-6 3.235326661227004E-5 1.1323643314294515E-4 34-35 0.26422733101870877 2.5882613289816034E-4 3.594807401363338E-6 3.594807401363338E-5 1.2042604794567182E-4 36-37 0.43731371258695206 2.6601574770088697E-4 3.594807401363338E-6 3.9542881414996717E-5 1.276156627483985E-4 38-39 0.6694933356225067 2.750027662042953E-4 3.594807401363338E-6 4.493509251704172E-5 1.3840008495248851E-4 40-41 0.9616900656275229 2.8039497730634035E-4 3.594807401363338E-6 5.032730361908673E-5 1.4738710345589686E-4 42-43 1.312839839611197 2.857871884083854E-4 3.594807401363338E-6 5.032730361908673E-5 1.5817152565998687E-4 44-45 1.7231223979435977 2.911793995104304E-4 3.594807401363338E-6 5.032730361908673E-5 1.5817152565998687E-4 46-47 2.19302663443131 3.0915343651724704E-4 3.594807401363338E-6 5.032730361908673E-5 1.617663330613502E-4 48-49 2.7204064490557194 3.2173526242201876E-4 3.594807401363338E-6 5.3922111020450066E-5 1.6895594786407687E-4 50-51 3.2992692978787543 3.3970929942883546E-4 3.594807401363338E-6 5.3922111020450066E-5 1.8333517746953025E-4 52-53 3.9257741291920576 3.540885290342888E-4 3.594807401363338E-6 6.111172582317674E-5 1.9411959967362025E-4 54-55 4.594352586330917 3.5948074013633375E-4 3.594807401363338E-6 6.470653322454008E-5 2.031066181770286E-4 56-57 5.305932129604885 3.8644179564655885E-4 3.594807401363338E-6 6.470653322454008E-5 2.0670142557839192E-4 58-59 6.056797525564653 4.0082102525201217E-4 3.594807401363338E-6 7.189614802726676E-5 2.1568844408180026E-4 60-61 6.843783546293318 4.1879506225882886E-4 3.594807401363338E-6 7.189614802726676E-5 2.1568844408180026E-4 62-63 7.661431476751913 4.421613103676906E-4 3.594807401363338E-6 7.549095542863009E-5 2.318650773879353E-4 64-65 8.505476077958718 4.5654053997314396E-4 3.594807401363338E-6 7.908576282999343E-5 2.462443069933887E-4 66-67 9.371883976009407 4.655275584765523E-4 3.594807401363338E-6 7.908576282999343E-5 2.570287291974786E-4 68-69 10.26420684061652 4.745145769799606E-4 3.594807401363338E-6 7.908576282999343E-5 2.5882613289816034E-4 70-71 11.179917522176906 4.745145769799606E-4 3.594807401363338E-6 7.908576282999343E-5 2.66015747700887E-4 72-73 12.107640252668947 4.888938065854139E-4 3.594807401363338E-6 8.268057023135678E-5 2.7320536250361366E-4 74-75 13.051555793100428 5.014756324901856E-4 3.594807401363338E-6 8.268057023135678E-5 2.839897847077037E-4 76-77 14.007643351392925 5.194496694970023E-4 3.594807401363338E-6 8.627537763272011E-5 2.839897847077037E-4 78-79 14.975759135250387 5.248418805990473E-4 3.594807401363338E-6 8.807278133340178E-5 2.857871884083854E-4 80-81 15.956214095513026 5.320314954017739E-4 3.594807401363338E-6 9.526239613612845E-5 2.9117939951043035E-4 82-83 16.947196449250555 5.42815917605864E-4 3.594807401363338E-6 1.0065460723817346E-4 2.92976803211112E-4 84-85 17.94835750014505 5.625873583133624E-4 3.594807401363338E-6 1.0065460723817346E-4 3.0196382171452036E-4 86-87 18.958333018787684 5.769665879188158E-4 3.594807401363338E-6 1.0065460723817346E-4 3.091534365172471E-4 88 19.721120593494074 5.895484138235874E-4 3.594807401363338E-6 1.0065460723817346E-4 3.127482439186104E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 36510 0.0 68.681816 8 GTATCAA 39475 0.0 63.463734 9 GTGGTAT 39590 0.0 63.445114 6 TGGTATC 40030 0.0 62.618893 7 AGTGGTA 40910 0.0 61.535664 5 GCAGTGG 50150 0.0 50.57231 3 AAGCAGT 51115 0.0 49.838184 1 CAGTGGT 51750 0.0 48.936172 4 AGCAGTG 57470 0.0 44.490192 2 CAACGCA 33030 0.0 40.324875 12-13 TCAACGC 32570 0.0 38.43036 12-13 ATCAACG 34855 0.0 38.307724 10-11 ACGCAGA 36940 0.0 36.270035 14-15 AACGCAG 34775 0.0 35.953266 14-15 AGAGTAC 38725 0.0 34.1869 18-19 CGCAGAG 38320 0.0 32.633347 16-17 GAGTACT 35955 0.0 32.555626 20-21 TATCAAC 40610 0.0 30.983679 10-11 AGTACTT 45745 0.0 27.468527 20-21 GTACTTT 42820 0.0 27.232134 22-23 >>END_MODULE