FastQCFastQC Report
Thu 26 May 2016
SRR1660666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27817902
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3914221.4070867026564404No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391590.14076906302998696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA721900.077.0580148
GTGGTAT747350.074.557696
GTATCAA748950.074.058499
AGTGGTA759100.073.556125
TGGTATC756200.073.45197
AAGCAGT838600.067.588351
GCAGTGG841300.066.9193653
CAGTGGT861350.065.0988854
AGCAGTG910400.062.2797852
CAACGCA690550.042.37074712-13
ATCAACG710100.041.27921710-11
TCAACGC683800.040.489912-13
ACGCAGA725100.040.44748714-15
AACGCAG707350.039.08279814-15
AGAGTAC748350.038.7812418-19
GTACTTT778250.037.55545822-23
CGCAGAG743850.037.04652816-17
GAGTACT715900.036.99312220-21
TATCAAC759900.036.5416910-11
AGTACTT812400.034.711220-21
Produced by FastQC (version 0.11.5)