##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660666_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27817902 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11983689496066 34.0 31.0 34.0 30.0 34.0 2 32.482042714795675 34.0 31.0 34.0 31.0 34.0 3 32.58935817661591 34.0 31.0 34.0 31.0 34.0 4 36.09722548450994 37.0 35.0 37.0 35.0 37.0 5 36.00935929675789 37.0 35.0 37.0 35.0 37.0 6 36.000737906115276 37.0 35.0 37.0 35.0 37.0 7 35.94930616981827 37.0 35.0 37.0 35.0 37.0 8 35.974922587620014 37.0 35.0 37.0 35.0 37.0 9 37.6939826734597 39.0 38.0 39.0 35.0 39.0 10-11 37.650742730346806 39.0 37.0 39.0 35.0 39.0 12-13 37.674154794276006 39.0 37.5 39.0 35.0 39.0 14-15 39.10914043769368 41.0 39.0 41.0 36.0 41.0 16-17 39.074512664542425 40.5 38.0 41.0 36.0 41.0 18-19 39.06963833936865 40.0 38.5 41.0 35.0 41.0 20-21 39.03330500984582 40.0 39.0 41.0 35.0 41.0 22-23 38.96037684653573 40.0 38.5 41.0 35.0 41.0 24-25 38.94278678888149 40.0 38.0 41.0 35.0 41.0 26-27 38.880587346234805 40.0 38.0 41.0 35.0 41.0 28-29 35.344479213421636 40.0 36.0 41.0 18.5 41.0 30-31 35.63424781279336 40.0 34.0 41.0 23.0 41.0 32-33 38.0407669313092 40.0 37.5 41.0 33.5 41.0 34-35 38.46942592938892 40.0 38.0 41.0 34.0 41.0 36-37 38.49259697945589 40.0 38.0 41.0 34.0 41.0 38-39 38.360411939045576 40.0 38.0 41.0 34.0 41.0 40-41 38.27375105426714 40.0 38.0 41.0 33.5 41.0 42-43 38.173146918124885 40.0 38.0 41.0 33.0 41.0 44-45 38.081999587891275 40.0 37.5 41.0 33.0 41.0 46-47 37.97061557697629 40.0 37.0 41.0 33.0 41.0 48-49 37.85477671896321 40.0 37.0 41.0 33.0 41.0 50-51 37.67559005707907 40.0 37.0 41.0 33.0 41.0 52-53 37.48043310383363 40.0 36.0 41.0 32.0 41.0 54-55 37.241109124620536 39.5 36.0 41.0 31.5 41.0 56-57 37.00689987332618 39.0 36.0 41.0 31.0 41.0 58-59 36.78615364307488 39.0 35.0 41.0 31.0 41.0 60-61 36.53983443826928 39.0 35.0 41.0 30.5 41.0 62-63 36.27795634983544 38.0 35.0 40.0 30.0 41.0 64-65 35.9880568994743 37.5 35.0 40.0 30.0 41.0 66-67 35.67383816364009 37.0 35.0 40.0 29.5 41.0 68-69 35.34554528950458 36.5 34.0 39.0 29.0 41.0 70-71 35.062057465728365 36.0 34.0 39.0 29.0 41.0 72-73 34.71957097986757 35.5 34.0 39.0 29.0 40.0 74-75 34.36151523935917 35.0 34.0 37.5 29.0 40.0 76-77 33.48321997827155 35.0 32.5 36.5 27.5 39.0 78-79 33.81736895543021 35.0 34.0 36.5 29.0 39.0 80-81 33.63671014801907 35.0 34.0 36.0 29.0 38.5 82-83 33.40319595273576 35.0 34.0 36.0 29.0 37.0 84-85 33.20761305076134 35.0 34.0 35.0 29.0 37.0 86-87 33.081758969457866 35.0 33.5 35.0 29.0 36.5 88-89 32.93377584693483 35.0 33.0 35.0 28.5 36.0 90-91 32.808188068963645 35.0 33.0 35.0 27.5 36.0 92-93 32.71128052719432 35.0 33.0 35.0 27.0 36.0 94-95 32.61807615829548 35.0 33.0 35.0 27.0 35.0 96-97 32.5434264057728 35.0 33.0 35.0 27.0 35.0 98-99 32.47613234815479 35.0 33.0 35.0 27.0 35.0 100 32.42062000218421 35.0 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1404414652170871 1101 2 0.02807659459917744 1101 3 -0.09652285575240427 1101 4 -0.07887921175625934 1101 5 -0.08265907170427056 1101 6 -0.07149662833484882 1101 7 -0.06019598514208013 1101 8 -0.07409689947906628 1101 9 -0.10648231353433601 1101 10-11 -0.10697450168671452 1101 12-13 -0.1289894491997856 1101 14-15 -0.15437206926223723 1101 16-17 -0.1420104776293698 1101 18-19 -0.14164647334606428 1101 20-21 -0.1282708273997315 1101 22-23 -0.15032846060709915 1101 24-25 -0.15262194540915175 1101 26-27 -0.16620800563023153 1101 28-29 2.540800564781989 1101 30-31 2.247063762034564 1101 32-33 0.32467739813842655 1101 34-35 -0.06000413862809495 1101 36-37 -0.167890248657109 1101 38-39 -0.20202996124223205 1101 40-41 -0.2265349619584569 1101 42-43 -0.29335850920860906 1101 44-45 -0.26873460411153616 1101 46-47 -0.22533921802429546 1101 48-49 -0.2372898516595896 1101 50-51 -0.22654066970375908 1101 52-53 -0.22754075639657145 1101 54-55 -0.2195681511554497 1101 56-57 -0.25568563312786097 1101 58-59 -0.24398167072996557 1101 60-61 -0.27974597878791485 1101 62-63 -0.28694759680507076 1101 64-65 -0.2802877351684927 1101 66-67 -0.27699127131060663 1101 68-69 -0.23182766415477118 1101 70-71 -0.2779767120452803 1101 72-73 -0.29061840746766165 1101 74-75 -0.29992738735778346 1101 76-77 -0.07644652588531642 1101 78-79 -0.2513712391598446 1101 80-81 -0.25059746173934627 1101 82-83 -0.25666628380340484 1101 84-85 -0.2489245014304018 1101 86-87 -0.2324188153101403 1101 88-89 -0.29543473691003186 1101 90-91 -0.27949435708312365 1101 92-93 -0.29959261735519505 1101 94-95 -0.3420585081646479 1101 96-97 -0.33582454732239597 1101 98-99 -0.2918358869683715 1101 100 -0.32062157396985924 1102 1 0.11867954653683199 1102 2 0.028099038847983593 1102 3 -0.09504122601732945 1102 4 -0.06077549399559956 1102 5 -0.08009981413918155 1102 6 -0.11302955880694299 1102 7 -0.09879163308285399 1102 8 -0.11743473609681132 1102 9 -0.16311346196020082 1102 10-11 -0.1428897088558685 1102 12-13 -0.14605698718952453 1102 14-15 -0.2464448626032194 1102 16-17 -0.19281886021333605 1102 18-19 -0.19899149919965708 1102 20-21 -0.21508069673068064 1102 22-23 -0.21279897471941922 1102 24-25 -0.206872861170865 1102 26-27 -0.20470660769974103 1102 28-29 2.472028502223793 1102 30-31 2.204793226504698 1102 32-33 0.24770472747349004 1102 34-35 -0.13754630768696785 1102 36-37 -0.2518258947877925 1102 38-39 -0.26696730233697963 1102 40-41 -0.3194273909392038 1102 42-43 -0.2714749406377379 1102 44-45 -0.2831122184174788 1102 46-47 -0.2560384572000771 1102 48-49 -0.2303192410250503 1102 50-51 -0.21457117786292912 1102 52-53 -0.24350753042120488 1102 54-55 -0.26920431284290913 1102 56-57 -0.22806209715519543 1102 58-59 -0.34717770028591843 1102 60-61 -0.3444712743690701 1102 62-63 -0.37621961542442506 1102 64-65 -0.35962527048196335 1102 66-67 -0.3267959493671242 1102 68-69 -0.32256397736586706 1102 70-71 -0.3350176986406268 1102 72-73 -0.37428724053614104 1102 74-75 -0.36824195027715234 1102 76-77 -0.11951836885128841 1102 78-79 -0.30552033639149556 1102 80-81 -0.28028328284671744 1102 82-83 -0.3015841960797516 1102 84-85 -0.29085600996165795 1102 86-87 -0.2757864355979933 1102 88-89 -0.28876512666059995 1102 90-91 -0.37590974898863294 1102 92-93 -0.2919616243134229 1102 94-95 -0.33408229913350596 1102 96-97 -0.34242115135777595 1102 98-99 -0.32315265230019463 1102 100 -0.3961457603232432 1103 1 0.11604480625943125 1103 2 0.011378667075575777 1103 3 -0.10834122221150011 1103 4 -0.09114932866387448 1103 5 -0.06413961625288778 1103 6 -0.10298749127000661 1103 7 -0.08782300491030526 1103 8 -0.08386256176692797 1103 9 -0.12782981266063587 1103 10-11 -0.1605099445563667 1103 12-13 -0.1601317868871206 1103 14-15 -0.23884817961255322 1103 16-17 -0.2637234893312126 1103 18-19 -0.2872796475066295 1103 20-21 -0.27303321523582724 1103 22-23 -0.3219904666022586 1103 24-25 -0.31353412564811833 1103 26-27 -0.32579163435460856 1103 28-29 2.4100835483204364 1103 30-31 2.084103712932823 1103 32-33 0.10252188094347048 1103 34-35 -0.23818031275305174 1103 36-37 -0.37296724241974033 1103 38-39 -0.36413884265888896 1103 40-41 -0.30683368455814986 1103 42-43 -0.3859279733378358 1103 44-45 -0.36061293971196307 1103 46-47 -0.36719450250423336 1103 48-49 -0.37334394636411616 1103 50-51 -0.35378568944997113 1103 52-53 -0.3107206449275779 1103 54-55 -0.32665354465718366 1103 56-57 -0.43988836245077323 1103 58-59 -0.4006598807402284 1103 60-61 -0.3289509344837782 1103 62-63 -0.27259667968154844 1103 64-65 -0.22604202002703744 1103 66-67 -0.20703270148990782 1103 68-69 -0.23098219798172437 1103 70-71 -0.2692574659847935 1103 72-73 -0.23650777324436234 1103 74-75 -0.22517992034293144 1103 76-77 0.04294968139721078 1103 78-79 -0.1713882399720532 1103 80-81 -0.2022294571501817 1103 82-83 -0.22903579095363824 1103 84-85 -0.2178423846437738 1103 86-87 -0.2193194108555332 1103 88-89 -0.22202585026567334 1103 90-91 -0.23615961280238196 1103 92-93 -0.25381255085985543 1103 94-95 -0.22458011335889694 1103 96-97 -0.2616187873339655 1103 98-99 -0.2783771669224251 1103 100 -0.2813903448760513 1104 1 0.024254608218420515 1104 2 -0.06599389187907434 1104 3 -0.17558128287907238 1104 4 -0.1289148468151211 1104 5 -0.16937771110135458 1104 6 -0.16883270616181534 1104 7 -0.14688254550517144 1104 8 -0.15308986855723106 1104 9 -0.203325628666434 1104 10-11 -0.21126816808388327 1104 12-13 -0.2120504427472838 1104 14-15 -0.2919777617514612 1104 16-17 -0.28817555017829477 1104 18-19 -0.2831524910662395 1104 20-21 -0.2943682375046208 1104 22-23 -0.2848562801603265 1104 24-25 -0.2914672061110153 1104 26-27 -0.30511921055929037 1104 28-29 2.374576182321526 1104 30-31 2.0964936879917744 1104 32-33 0.1878099789498222 1104 34-35 -0.2084713356328578 1104 36-37 -0.30581403779349614 1104 38-39 -0.3484823671527977 1104 40-41 -0.33287022760595875 1104 42-43 -0.3634391543933546 1104 44-45 -0.32795415649965776 1104 46-47 -0.3191971822163353 1104 48-49 -0.30143491984204474 1104 50-51 -0.3302414880464042 1104 52-53 -0.30149500888389724 1104 54-55 -0.287562965340463 1104 56-57 -0.3244550867800058 1104 58-59 -0.3332928917521514 1104 60-61 -0.3427561648570645 1104 62-63 -0.3385857232755427 1104 64-65 -0.3364325261642662 1104 66-67 -0.35388169885783327 1104 68-69 -0.33565024704694935 1104 70-71 -0.4160127961965969 1104 72-73 -0.42557013717635783 1104 74-75 -0.3931212200624117 1104 76-77 -0.06117298561477469 1104 78-79 -0.2551587078613764 1104 80-81 -0.2920456883176641 1104 82-83 -0.3634841015171375 1104 84-85 -0.31548496144102245 1104 86-87 -0.3381303417219286 1104 88-89 -0.3075566417383371 1104 90-91 -0.3618267255681218 1104 92-93 -0.35751154537037877 1104 94-95 -0.38483343844198714 1104 96-97 -0.37013279210751904 1104 98-99 -0.3967421245071918 1104 100 -0.40037295903865555 1105 1 0.018369163023962187 1105 2 -0.06438769631094488 1105 3 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-0.3623589321871066 1105 62-63 -0.38620399953919815 1105 64-65 -0.345072413761919 1105 66-67 -0.3152079217297654 1105 68-69 -0.27783203594221817 1105 70-71 -0.3234630381248067 1105 72-73 -0.3443255778322154 1105 74-75 -0.35642068931654336 1105 76-77 -0.07527895434249388 1105 78-79 -0.2680735168846411 1105 80-81 -0.290319383244551 1105 82-83 -0.3307784890244321 1105 84-85 -0.29140213561023387 1105 86-87 -0.28480249971261173 1105 88-89 -0.2947086283050524 1105 90-91 -0.33810850756058386 1105 92-93 -0.3509627230504435 1105 94-95 -0.3510678592520051 1105 96-97 -0.36777088650264744 1105 98-99 -0.37156196676870934 1105 100 -0.3839835692492741 1106 1 0.0038896743432914604 1106 2 -0.10921099948827617 1106 3 -0.2251804239231845 1106 4 -0.17566179918100033 1106 5 -0.19466033606074262 1106 6 -0.18569083276490517 1106 7 -0.17591741162586771 1106 8 -0.19275320374377003 1106 9 -0.2542289560126392 1106 10-11 -0.25039086235528174 1106 12-13 -0.2410859573557289 1106 14-15 -0.3221571576825397 1106 16-17 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-0.39067121402867855 1106 82-83 -0.42426198624471567 1106 84-85 -0.4291053344232907 1106 86-87 -0.4070129084358882 1106 88-89 -0.3975919923883211 1106 90-91 -0.43893295262165566 1106 92-93 -0.45696041520849207 1106 94-95 -0.45488336309664845 1106 96-97 -0.45399841076443437 1106 98-99 -0.4864632419944144 1106 100 -0.47067046559186565 1107 1 -0.06342296144316606 1107 2 -0.14049159807036204 1107 3 -0.23907058653491475 1107 4 -0.197142987741465 1107 5 -0.2547455524842448 1107 6 -0.24546081755847382 1107 7 -0.2252153573059701 1107 8 -0.2756586975896411 1107 9 -0.30597149402888135 1107 10-11 -0.324513577303982 1107 12-13 -0.3179933810970397 1107 14-15 -0.4131368767109578 1107 16-17 -0.39391966285963065 1107 18-19 -0.38149423830675744 1107 20-21 -0.3814503076783211 1107 22-23 -0.37204927287345413 1107 24-25 -0.3817035603084378 1107 26-27 -0.4109208005395999 1107 28-29 2.2669520312714866 1107 30-31 2.0101040897576254 1107 32-33 0.08344174363386259 1107 34-35 -0.322211932038158 1107 36-37 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0.7154511011154909 2310 40-41 0.7181215602722588 2310 42-43 0.7107838700407356 2310 44-45 0.7229209033826862 2310 46-47 0.7396015878558941 2310 48-49 0.6441302421694601 2310 50-51 0.7544272007040647 2310 52-53 0.7069663966451643 2310 54-55 0.7229710244209997 2310 56-57 0.7734800802167285 2310 58-59 0.7979607763850183 2310 60-61 0.7778223748279416 2310 62-63 0.7661503498058053 2310 64-65 0.7910012466925309 2310 66-67 0.8666848152484476 2310 68-69 0.8597625508685027 2310 70-71 0.92294797798845 2310 72-73 0.9071518707491819 2310 74-75 0.8472045034898699 2310 76-77 0.4413574117826755 2310 78-79 0.664294854725 2310 80-81 0.6811564914916062 2310 82-83 0.6904792469194021 2310 84-85 0.6636524346188892 2310 86-87 0.5878551812852706 2310 88-89 0.5964920693391278 2310 90-91 0.5639399189303873 2310 92-93 0.6230849281105222 2310 94-95 0.6700560070620085 2310 96-97 0.6566574566227743 2310 98-99 0.6161715993746668 2310 100 0.6168082458472881 2311 1 0.20261177313652468 2311 2 0.28369994936086584 2311 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0.8117854649298977 2311 66-67 0.826093415339443 2311 68-69 0.8103226452691814 2311 70-71 0.8823568838468816 2311 72-73 0.9123728612196658 2311 74-75 0.7853728130238053 2311 76-77 0.2968146208649287 2311 78-79 0.6053272185809533 2311 80-81 0.6745196189575324 2311 82-83 0.6535325890520696 2311 84-85 0.6180099817022224 2311 86-87 0.5862606502715906 2311 88-89 0.6101098143086929 2311 90-91 0.5985467463179504 2311 92-93 0.6005424202064873 2311 94-95 0.5901219672128235 2311 96-97 0.5762195351194777 2311 98-99 0.5319564808389003 2311 100 0.5397818177564488 2312 1 0.77289006190734 2312 2 0.6121465542184126 2312 3 0.5157551048886049 2312 4 0.34219139718434377 2312 5 0.38971155539465485 2312 6 0.3712478177525682 2312 7 0.3776413290720697 2312 8 0.33512399475976906 2312 9 0.4353606913449184 2312 10-11 0.48460349897897714 2312 12-13 0.45843275473140466 2312 14-15 0.6010245664703859 2312 16-17 0.6030079643868476 2312 18-19 0.6108992861905449 2312 20-21 0.597113040483535 2312 22-23 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0.6194812402235002 2312 90-91 0.6427936512968415 2312 92-93 0.6635745743481678 2312 94-95 0.6669674492534554 2312 96-97 0.6495525049168123 2312 98-99 0.622497117025361 2312 100 0.6330817637248458 2313 1 0.7177349864939444 2313 2 0.5244904802607735 2313 3 0.48740077225999556 2313 4 0.3156418877544169 2313 5 0.34258288362793365 2313 6 0.35283771958012977 2313 7 0.360012180477554 2313 8 0.36413618500416334 2313 9 0.45603141088577814 2313 10-11 0.42061891862443446 2313 12-13 0.45085559079865334 2313 14-15 0.5584782215244104 2313 16-17 0.5880689894156461 2313 18-19 0.5459185774820696 2313 20-21 0.5938215638343394 2313 22-23 0.5655346230497145 2313 24-25 0.5623916950883796 2313 26-27 0.5749858056951993 2313 28-29 3.2898659555135907 2313 30-31 3.04940696034528 2313 32-33 1.0849832799588697 2313 34-35 0.6830938739280086 2313 36-37 0.6451810180534707 2313 38-39 0.6112777735071688 2313 40-41 0.6443631926764439 2313 42-43 0.6589464651962018 2313 44-45 0.6594467327112952 2313 46-47 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2314 72-73 0.7630814518837994 2314 74-75 0.7375216290582998 2314 76-77 0.13169116019254545 2314 78-79 0.5135021343677479 2314 80-81 0.19719377841173014 2314 82-83 0.19088324505925414 2314 84-85 0.46121838041238306 2314 86-87 0.5221370806395669 2314 88-89 0.5318222989481924 2314 90-91 0.5295063355851397 2314 92-93 0.5617270623658825 2314 94-95 0.5392412822915915 2314 96-97 0.5008597064337721 2314 98-99 0.45605458723963466 2314 100 0.4374782275624156 2315 1 0.6343093960351212 2315 2 0.4777070877069747 2315 3 0.4380169051746776 2315 4 0.2688340457187479 2315 5 0.28308013439203705 2315 6 0.3007860864374834 2315 7 0.30994606109369016 2315 8 0.3153338717931291 2315 9 0.38772740694956553 2315 10-11 0.38562431593712887 2315 12-13 0.391393617002727 2315 14-15 0.5045163851693815 2315 16-17 0.46408801285283374 2315 18-19 0.454061972208855 2315 20-21 0.4700977930909005 2315 22-23 0.4713564601360076 2315 24-25 0.45451215258407274 2315 26-27 0.5045100185221187 2315 28-29 3.226086860950076 2315 30-31 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0.4580408098988116 2315 98-99 0.4929446470232648 2315 100 0.5182885741803673 2316 1 0.5189815648925702 2316 2 0.1230244198099939 2316 3 0.20158968292651025 2316 4 0.2080274303255223 2316 5 0.24484216059970265 2316 6 0.23834481967381294 2316 7 0.2738436981917829 2316 8 0.2755994223812337 2316 9 0.2982198860646932 2316 10-11 0.36765714522386617 2316 12-13 0.31793150820752913 2316 14-15 0.40282490807432225 2316 16-17 0.4442545335740533 2316 18-19 0.430547173602271 2316 20-21 0.4055967330848631 2316 22-23 0.38373968595342944 2316 24-25 0.4131747693835166 2316 26-27 0.4598398082810533 2316 28-29 3.165579557022369 2316 30-31 2.9019378948644814 2316 32-33 0.9796448587630877 2316 34-35 0.5746580812290674 2316 36-37 0.4613119154315726 2316 38-39 0.4561964574932347 2316 40-41 0.4987927513878603 2316 42-43 0.4712702361098593 2316 44-45 0.4931341918660692 2316 46-47 0.42590135805011897 2316 48-49 0.42667813610416516 2316 50-51 0.4970864415352523 2316 52-53 0.4389792038249354 2316 54-55 0.4568992860685839 2316 56-57 0.4751482962105058 2316 58-59 0.4724586562376203 2316 60-61 0.4791747039313208 2316 62-63 0.5067999537779926 2316 64-65 0.4807396992922861 2316 66-67 0.47899339170866995 2316 68-69 0.4566741996214958 2316 70-71 0.523431243186856 2316 72-73 0.5654305232527648 2316 74-75 0.5962306512848912 2316 76-77 0.08576020343815571 2316 78-79 0.3737220078316241 2316 80-81 0.44789458088239087 2316 82-83 0.4422102154638523 2316 84-85 0.4400045531556387 2316 86-87 0.4272780831624061 2316 88-89 0.3959934771063658 2316 90-91 0.381053378438196 2316 92-93 0.4195844684103136 2316 94-95 0.4462349068373115 2316 96-97 0.4576743715511924 2316 98-99 0.4279698214239076 2316 100 0.4481878902453147 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 33.0 8 226.0 9 285.0 10 184.0 11 133.0 12 181.0 13 674.0 14 2090.0 15 5694.0 16 11432.0 17 15588.0 18 20313.0 19 28158.0 20 41080.0 21 58949.0 22 86673.0 23 131151.0 24 190306.0 25 247914.0 26 289256.0 27 312047.0 28 354189.0 29 418994.0 30 506984.0 31 619437.0 32 769253.0 33 979488.0 34 1319838.0 35 1982907.0 36 3428840.0 37 6466024.0 38 7498025.0 39 2027462.0 40 4094.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.13563972200863 30.790563138743458 26.973387021890037 24.10041011735787 2 22.01705332985771 28.554670070381388 28.618029500858423 20.810247098902483 3 21.511026281484746 27.20399974372786 27.120711297567553 24.164262677219845 4 20.117145141497357 27.830911424732058 27.452042485460478 24.599900948310108 5 22.085891114334856 28.970696740353645 27.243067816597915 21.700344328713587 6 23.895735139280095 26.751184925187054 27.53692188669618 21.816158048836673 7 21.935036653734706 27.294402000553458 28.970056764165754 21.800504581546086 8 23.16712453728538 26.664577364604998 27.75426414256546 22.414033955544166 9 23.293920583946264 26.241116242339196 28.163169889663138 22.3017932840514 10-11 21.679240943475893 28.045479490149905 28.247620902539666 22.027658663834533 12-13 21.760449440076393 27.061972538403506 28.03589573361787 23.141682287902228 14-15 21.740750223131766 29.15883530611004 27.01038592628477 22.090028544473427 16-17 22.90277702660791 26.937237722264122 27.019615068747278 23.140370182380686 18-19 21.87851909177047 27.972652287005683 26.985083202895748 23.163745418328098 20-21 22.958377912753004 27.94862114226066 26.969052708489016 22.123948236497323 22-23 22.936850823060006 26.96076804015376 28.007208747859313 22.095172388926922 24-25 21.997838651512097 27.875968845103976 26.851365778222338 23.27482672516159 26-27 22.04791218526203 26.84946543127007 28.995815418201115 22.106806965266784 28-29 22.104866109288814 26.940247233952757 28.894136558787526 22.0607500979709 30-31 22.11307083029333 26.871905122072466 28.875780853936366 22.139243193697837 32-33 22.155341484297043 26.9315072453377 28.812385743499974 22.10076552686528 34-35 22.180628923471964 26.997196326946653 28.767313403885918 22.05486134569546 36-37 22.193396064419733 26.982833634047505 28.72860605362739 22.095164247905373 38-39 22.260418109575795 26.992877764577933 28.717992949181838 22.028711176664437 40-41 22.27429942708102 27.09740762751094 28.686188743458523 21.942104201949522 42-43 22.317328892014448 27.124010880514387 28.57406881705434 21.984591410416822 44-45 22.36427067666577 27.18506800946462 28.504850939364314 21.9458103745053 46-47 22.423793948853728 27.313209849437992 28.38347899231179 21.87951720939649 48-49 22.484323638444533 27.344305424444613 28.23822118216882 21.933149754942033 50-51 22.5394505592062 27.39518758350652 28.146115130156886 21.919246727130393 52-53 22.61373673909184 27.54293444431711 27.929364442676697 21.913964373914354 54-55 22.762051004172523 27.634886271118 27.546698161940558 22.056364562768923 56-57 22.937230457051434 27.851577233370755 27.094131223036495 22.11706108654132 58-59 22.978737356845194 28.048947534918494 26.85792371239276 22.11439139584355 60-61 22.978353011719054 28.12093531487152 26.70163492413684 22.19907674927258 62-63 22.94449850015696 28.1638918789956 26.645518875907868 22.24609074493957 64-65 22.947518747207965 28.24176547370782 26.550357521659883 22.260358257424333 66-67 22.90414082083888 28.160270351803472 26.5209306869176 22.41465814044005 68-69 22.837713373295056 28.09583400355256 26.56626728671263 22.500185336439745 70-71 22.772365742999455 28.067301678773955 26.58925352906532 22.571079049161266 72-73 22.724799752004312 27.9899747556815 26.559444198579623 22.72578129373456 74-75 22.681122576424144 27.952783812429022 26.541769325448904 22.824324285697926 76-77 22.668527095103332 27.961831314523387 26.4550334938242 22.91460809654908 78-79 22.635759274627585 27.925812579669056 26.363962405598297 23.07446574010506 80-81 22.586341903588632 27.905313418301507 26.32222518709196 23.186119491017898 82-83 22.56536967740074 27.876970060925498 26.241497813932245 23.316162447741515 84-85 22.51862500642229 27.794484425946326 26.21691243236729 23.469978135264093 86-87 22.47835876654915 27.662241635088215 26.255637188673653 23.603762409688986 88-89 22.426144190342217 27.570333184272677 26.30077324278835 23.702749382596757 90-91 22.40099103346904 27.402769699017576 26.3383873993249 23.85785186818848 92-93 22.355501356077557 27.254812858277255 26.434381802724204 23.95530398292098 94-95 22.330915533899947 27.161925447548768 26.496670462230664 24.01048855632062 96-97 22.27658694288254 27.02896472999233 26.548259397794638 24.146188929330492 98-99 22.225525898989147 26.941953847893245 26.63555685401498 24.19696339910263 100 22.180818996443673 26.940148484234083 26.62946009384185 24.249572425480395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 61340.0 1 37402.0 2 9404.5 3 4544.0 4 3610.0 5 3288.0 6 3168.0 7 3286.5 8 3237.5 9 3198.0 10 3403.5 11 5647.5 12 52196.5 13 59699.5 14 20531.5 15 18944.0 16 21158.0 17 24681.5 18 29143.5 19 34400.0 20 42110.5 21 54774.5 22 72530.0 23 93128.5 24 115331.5 25 142773.0 26 174849.5 27 210132.0 28 253526.0 29 299814.0 30 343221.0 31 388923.5 32 437097.0 33 484776.0 34 531208.5 35 586389.5 36 650994.5 37 701698.0 38 745359.5 39 788979.0 40 824778.5 41 861657.0 42 902468.5 43 934678.5 44 963969.5 45 999962.0 46 1028874.5 47 1047942.5 48 1061361.0 49 1072925.5 50 1083138.5 51 1084164.0 52 1066232.5 53 1026050.5 54 968939.0 55 900433.0 56 821140.5 57 732781.5 58 637813.5 59 537960.5 60 437451.0 61 346284.5 62 266869.5 63 197919.5 64 143989.5 65 103355.5 66 73059.0 67 52232.5 68 37810.0 69 27919.0 70 21267.5 71 16532.0 72 12983.0 73 9781.0 74 7400.0 75 5796.5 76 4143.5 77 2493.0 78 1516.5 79 979.5 80 612.5 81 400.5 82 246.0 83 140.0 84 87.0 85 53.0 86 29.0 87 15.5 88 12.0 89 8.5 90 4.5 91 3.5 92 1.5 93 1.5 94 0.5 95 1.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.931792052470384 2 0.018520447731823916 3 0.013372683533071616 4 3.702651623404238E-4 5 2.5163651809543364E-5 6 0.001161122790640358 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.274603095517411E-4 16-17 6.524575433474459E-4 18-19 0.0 20-21 1.4199489235385183E-4 22-23 1.2042604794567182E-4 24-25 0.005289759091106152 26-27 0.01399099040610611 28-29 9.685743015415037 30-31 0.022009208314847036 32-33 0.025598623505108328 34-35 0.03115979055501741 36-37 0.03670657837532104 38-39 0.03414348069814898 40-41 0.0430064783462103 42-43 0.043308442167924815 44-45 0.04628314529255297 46-47 0.016095750139604344 48-49 0.032617484956270246 50-51 0.0030106511986417953 52-53 0.016947719493727455 54-55 0.01663856605721021 56-57 0.0161784307098357 58-59 0.012024630757560367 60-61 0.011674137035927441 62-63 0.012551270041860095 64-65 0.011839498176390153 66-67 0.01675359989405384 68-69 0.013013202792935281 70-71 0.017386285996693784 72-73 0.01623055541715547 74-75 0.016847064886489283 76-77 0.01909561691604205 78-79 0.022889936128181052 80-81 0.018604925705755955 82-83 0.017379096381891057 84-85 0.016618794616502713 86-87 0.016189215132039792 88-89 0.017397070418897875 90-91 0.01767207318510217 92-93 0.0165217348166659 94-95 0.017010628623251315 96-97 0.020294485184396725 98-99 0.019611471778137688 100 0.02081752966129509 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.7817902E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.99409697396348 #Duplication Level Percentage of deduplicated Percentage of total 1 76.52803256019855 38.25949881041201 2 11.375954606323575 11.374611555198946 3 4.288783684199708 6.432416022246978 4 2.2312950957482767 4.462063335774739 5 1.2957769363775287 3.2390598906941737 6 0.8464558584200712 2.5390677762019527 7 0.5973832636004228 2.0905945767743632 8 0.4418672541762337 1.7672603483924476 9 0.3342196369339543 1.5038108045531078 >10 1.8440457165810595 16.65472109638059 >50 0.15790544080110874 5.515150070640332 >100 0.0579087681181326 4.288240622299839 >500 2.3981652073861706E-4 0.0796946290401416 >1k 1.0225389028759804E-4 0.10714003924285787 >5k 0.0 0.0 >10k+ 2.910811039069743E-5 1.6866704221474875 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 391422 1.4070867026564404 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39159 0.14076906302998696 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.011183445825641344 3.594807401363338E-6 0.0 7.189614802726676E-6 0.0 2 0.011190635440444071 7.189614802726676E-6 0.0 1.4379229605453352E-5 0.0 3 0.011194230247845435 1.797403700681669E-5 0.0 1.4379229605453352E-5 7.189614802726676E-6 4 0.011205014670049524 2.1568844408180027E-5 0.0 1.4379229605453352E-5 1.4379229605453352E-5 5 0.011205014670049524 2.1568844408180027E-5 0.0 1.4379229605453352E-5 1.797403700681669E-5 6 0.011205014670049524 2.1568844408180027E-5 0.0 1.797403700681669E-5 2.1568844408180027E-5 7 0.011205014670049524 2.8758459210906705E-5 0.0 1.797403700681669E-5 2.1568844408180027E-5 8 0.011215799092253615 3.235326661227004E-5 0.0 1.797403700681669E-5 2.1568844408180027E-5 9 0.011219393899654978 3.594807401363338E-5 0.0 1.797403700681669E-5 2.5163651809543364E-5 10-11 0.01122298870705634 5.751691842181341E-5 0.0 1.797403700681669E-5 2.5163651809543364E-5 12-13 0.011237367936661795 7.009874432658509E-5 0.0 1.797403700681669E-5 3.4150670312951714E-5 14-15 0.011249949762566566 7.549095542863009E-5 0.0 1.797403700681669E-5 3.594807401363338E-5 16-17 0.011269721203274064 8.08831665306751E-5 0.0 1.9771440707498358E-5 3.9542881414996717E-5 18-19 0.011284100432879517 8.807278133340178E-5 0.0 2.5163651809543364E-5 4.673249621772339E-5 20-21 0.011336225140199286 1.0424941463953679E-4 0.0 2.6961055510225033E-5 5.2124707319768394E-5 22-23 0.011524952528770862 1.096416257415818E-4 0.0 2.8758459210906705E-5 5.751691842181341E-5 24-25 0.012606989556581226 1.2042604794567182E-4 0.0 2.8758459210906705E-5 6.470653322454008E-5 26-27 0.01736112234488424 1.330078738504435E-4 0.0 2.8758459210906705E-5 6.470653322454008E-5 28-29 0.031163385362418777 1.4558969975521518E-4 0.0 2.8758459210906705E-5 6.830134062590341E-5 30-31 0.06875967856957725 1.7255075526544022E-4 0.0 2.8758459210906705E-5 7.728835912931177E-5 32-33 0.14203623263896753 1.7974037006816688E-4 0.0 2.8758459210906705E-5 8.807278133340178E-5 34-35 0.26210819205560504 1.959170033743019E-4 0.0 3.235326661227004E-5 8.987018503408345E-5 36-37 0.43571761810074683 2.0130921447634694E-4 0.0 3.235326661227004E-5 9.166758873476512E-5 38-39 0.6688408780791593 2.192832514831636E-4 0.0 3.235326661227004E-5 9.526239613612845E-5 40-41 0.9619578787789245 2.3006767368725364E-4 0.0 3.235326661227004E-5 9.885720353749178E-5 42-43 1.313450956869429 2.534339217961153E-4 0.0 3.594807401363338E-5 1.0245201093885513E-4 44-45 1.723560964446564 2.60623536598842E-4 0.0 3.7745477714315045E-5 1.096416257415818E-4 46-47 2.1929331694388745 2.750027662042953E-4 1.797403700681669E-6 3.9542881414996717E-5 1.1143902944226348E-4 48-49 2.72016919176723 2.839897847077037E-4 3.594807401363338E-6 4.3137688816360054E-5 1.1683124054430848E-4 50-51 3.299202793941829 2.839897847077037E-4 3.594807401363338E-6 4.3137688816360054E-5 1.2042604794567182E-4 52-53 3.925903542258507 2.893819958097487E-4 3.594807401363338E-6 4.3137688816360054E-5 1.2581825904771682E-4 54-55 4.594679713804441 2.947742069117937E-4 3.594807401363338E-6 4.3137688816360054E-5 1.3121047014976183E-4 56-57 5.307713356672261 3.0196382171452036E-4 3.594807401363338E-6 4.673249621772339E-5 1.4019748865317017E-4 58-59 6.059360623241824 3.1634305131997373E-4 3.594807401363338E-6 4.673249621772339E-5 1.5098191085726018E-4 60-61 6.847092566506273 3.289248772247454E-4 3.594807401363338E-6 4.673249621772339E-5 1.5996892936066852E-4 62-63 7.664972362042256 3.3791189572815374E-4 3.594807401363338E-6 4.673249621772339E-5 1.6895594786407687E-4 64-65 8.50899179959725 3.5588593273497044E-4 3.594807401363338E-6 4.852989991840506E-5 1.7255075526544022E-4 66-67 9.37545182235526 3.630755475376971E-4 3.594807401363338E-6 5.032730361908673E-5 1.7614556266680356E-4 68-69 10.267812432440088 3.648729512383788E-4 3.594807401363338E-6 5.032730361908673E-5 1.797403700681669E-4 70-71 11.183880797336908 3.7385996974178713E-4 3.594807401363338E-6 5.032730361908673E-5 1.8333517746953025E-4 72-73 12.116204881302695 3.882391993472405E-4 3.594807401363338E-6 5.3922111020450066E-5 1.905247922722569E-4 74-75 13.066184861820277 3.9363141044928553E-4 3.594807401363338E-6 5.3922111020450066E-5 1.959170033743019E-4 76-77 14.030824466920619 4.0082102525201217E-4 3.594807401363338E-6 5.571951472113174E-5 1.977144070749836E-4 78-79 15.007422198841596 4.098080437554205E-4 3.594807401363338E-6 5.751691842181341E-5 1.977144070749836E-4 80-81 15.997133788162744 4.169976585581472E-4 3.594807401363338E-6 5.751691842181341E-5 2.0670142557839192E-4 82-83 16.998521311923525 4.223898696601922E-4 3.594807401363338E-6 6.470653322454008E-5 2.102962329797553E-4 84-85 18.01055845261084 4.385665029663272E-4 3.594807401363338E-6 6.470653322454008E-5 2.1748584778248192E-4 86-87 19.03181267947525 4.4755352146973556E-4 3.594807401363338E-6 6.470653322454008E-5 2.210806551838453E-4 88 19.80353155317033 4.493509251704172E-4 3.594807401363338E-6 6.470653322454008E-5 2.2287805888452695E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 72190 0.0 77.058014 8 GTGGTAT 74735 0.0 74.55769 6 GTATCAA 74895 0.0 74.05849 9 AGTGGTA 75910 0.0 73.55612 5 TGGTATC 75620 0.0 73.4519 7 AAGCAGT 83860 0.0 67.58835 1 GCAGTGG 84130 0.0 66.919365 3 CAGTGGT 86135 0.0 65.098885 4 AGCAGTG 91040 0.0 62.279785 2 CAACGCA 69055 0.0 42.370747 12-13 ATCAACG 71010 0.0 41.279217 10-11 TCAACGC 68380 0.0 40.4899 12-13 ACGCAGA 72510 0.0 40.447487 14-15 AACGCAG 70735 0.0 39.082798 14-15 AGAGTAC 74835 0.0 38.78124 18-19 GTACTTT 77825 0.0 37.555458 22-23 CGCAGAG 74385 0.0 37.046528 16-17 GAGTACT 71590 0.0 36.993122 20-21 TATCAAC 75990 0.0 36.54169 10-11 AGTACTT 81240 0.0 34.7112 20-21 >>END_MODULE