FastQCFastQC Report
Thu 26 May 2016
SRR1660665_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660665_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33112789
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3362761.0155471953751767No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1421140.429181607142787No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349640.1055906224027218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA540900.072.2915958
GTGGTAT564350.069.3544166
GTATCAA571450.068.310029
TGGTATC577050.067.699987
AGTGGTA580650.067.600155
GCAGTGG691350.057.3666233
AAGCAGT698750.057.0686261
CAGTGGT709250.055.6540764
AGCAGTG785350.050.871422
CAACGCA503350.040.9514912-13
TCAACGC492850.039.49113512-13
ATCAACG528450.039.11778310-11
ACGCAGA544800.038.0481714-15
AACGCAG519900.037.58461414-15
AGAGTAC558000.036.61566518-19
GAGTACT512900.035.62056420-21
CGCAGAG560700.034.8119316-17
TATCAAC585100.033.4736110-11
AGTACTT622050.031.1888820-21
GTACTTT602500.030.41241522-23
Produced by FastQC (version 0.11.5)