FastQCFastQC Report
Thu 26 May 2016
SRR1660665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33112789
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7931092.3951742633337227No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT913820.2759719213020685No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA653380.19731953113342401No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1079300.080.8586658
GTGGTAT1103050.079.325376
GTATCAA1107400.078.624459
AGTGGTA1114700.078.576535
TGGTATC1116650.078.170917
AAGCAGT1219800.072.550891
GCAGTGG1220050.072.192333
CAGTGGT1237000.071.085634
AGCAGTG1326500.066.8062442
CAACGCA1035200.043.76563612-13
ATCAACG1056550.042.9252710-11
TCAACGC1027050.042.2075112-13
ACGCAGA1085350.041.78088414-15
AACGCAG1054650.041.0323514-15
AGAGTAC1097000.041.028118-19
GAGTACT1056900.039.60123420-21
CGCAGAG1098100.039.3279116-17
TATCAAC1115450.038.99271410-11
AGTACTT1164700.037.8240820-21
GTACTTT1140150.036.5964122-23
Produced by FastQC (version 0.11.5)