FastQCFastQC Report
Thu 26 May 2016
SRR1660664_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660664_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47881786
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4789981.0003762182137483No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1935610.40424766110437066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA782850.071.9047248
GTGGTAT826250.068.453396
GTATCAA833050.067.378079
TGGTATC838700.067.354057
AGTGGTA845600.067.025285
GCAGTGG1004350.057.076033
AAGCAGT1024100.056.1360131
CAGTGGT1050350.054.272524
AGCAGTG1126650.051.2182542
CAACGCA715800.041.0902912-13
TCAACGC709700.039.459912-13
ATCAACG750350.039.33022710-11
ACGCAGA779150.038.02214-15
AACGCAG748850.037.43472714-15
AGAGTAC816400.036.021618-19
CGCAGAG795700.035.24850516-17
GAGTACT755050.034.85193620-21
TATCAAC843600.033.41132410-11
GTACTTT850900.030.82294822-23
AGTACTT929400.030.00808720-21
Produced by FastQC (version 0.11.5)