FastQCFastQC Report
Thu 26 May 2016
SRR1660664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47881786
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12729922.65861427975974No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1278780.2670702383574414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA741090.15477492840388202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1713650.081.9687048
GTGGTAT1759300.079.9896856
TGGTATC1767050.079.483647
GTATCAA1765050.079.3208169
AGTGGTA1781800.079.040435
AAGCAGT1923150.073.943411
GCAGTGG1922400.073.766283
CAGTGGT1973000.071.67194
AGCAGTG2048050.069.466922
CAACGCA1648700.044.0526412-13
ATCAACG1682750.043.1986710-11
TCAACGC1641850.042.46354312-13
ACGCAGA1715050.042.3750514-15
AACGCAG1680350.041.36961414-15
AGAGTAC1753800.041.2228418-19
CGCAGAG1735800.039.982216-17
GAGTACT1684350.039.92397320-21
TATCAAC1783450.039.2591310-11
AGTACTT1861150.037.99723420-21
GTACTTT1781650.037.5960322-23
Produced by FastQC (version 0.11.5)