FastQCFastQC Report
Thu 26 May 2016
SRR1660663_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660663_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24377124
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2813971.1543486426044351No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA775070.3179497302470956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA466300.069.3869868
AGTGGTA480150.067.634125
GTGGTAT479900.067.6320956
GTATCAA483900.066.822329
TGGTATC486350.066.567817
AAGCAGT567900.058.192081
GCAGTGG580500.056.653943
CAGTGGT588000.055.5800484
AGCAGTG643050.051.493772
CAACGCA441400.038.68889212-13
ATCAACG450950.037.9113410-11
TCAACGC433850.037.1122812-13
ACGCAGA468950.036.58078814-15
AACGCAG450300.035.8703214-15
GAGTACT429950.035.6573520-21
AGAGTAC483100.035.14832318-19
CGCAGAG479600.033.67901216-17
TATCAAC502300.032.29324710-11
AGTACTT505600.032.16338320-21
GTACTTT483350.031.66327522-23
Produced by FastQC (version 0.11.5)