FastQCFastQC Report
Thu 26 May 2016
SRR1660663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24377124
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6739972.764874970484623No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT434050.17805627932154755No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA331100.13582406193610042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA918550.080.222048
AGTGGTA935550.079.110215
GTGGTAT937550.078.826186
TGGTATC939750.078.45237
GTATCAA938200.078.406629
AAGCAGT1011650.073.97551
GCAGTGG1031350.072.319843
CAGTGGT1039100.071.4445344
AGCAGTG1090950.068.674782
CAACGCA886150.043.30108312-13
ATCAACG898500.042.79715710-11
ACGCAGA921850.041.60681514-15
TCAACGC882700.041.48750312-13
AACGCAG901100.040.4948114-15
AGAGTAC942800.040.37439318-19
GAGTACT885550.039.93374320-21
CGCAGAG934350.038.9461516-17
AGTACTT962600.038.72659320-21
TATCAAC952100.038.59642410-11
GTACTTT934850.037.77131322-23
Produced by FastQC (version 0.11.5)