FastQCFastQC Report
Thu 26 May 2016
SRR1660662_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660662_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42700833
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6119151.433028250291979No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1722170.4033106333077858No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT585250.13705821617109903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA919650.076.836918
TGGTATC964450.073.4613347
GTATCAA960500.073.398659
AGTGGTA970900.073.131275
GTGGTAT971700.072.9983146
AAGCAGT1111800.064.7298051
GCAGTGG1124600.063.6749843
CAGTGGT1149350.062.1363074
AGCAGTG1262750.057.096082
CAACGCA867100.042.76892512-13
ATCAACG891700.041.66118610-11
TCAACGC857750.041.04902312-13
ACGCAGA920450.040.50382614-15
AACGCAG889150.039.62142614-15
AGAGTAC954050.038.82769418-19
GAGTACT887750.037.47643720-21
CGCAGAG944700.037.27660816-17
TATCAAC977950.036.1414210-11
AGTACTT1020700.034.4849220-21
GTACTTT985450.033.60918422-23
Produced by FastQC (version 0.11.5)