FastQCFastQC Report
Thu 26 May 2016
SRR1660662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42700833
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12150172.8454175589501967No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1202710.2816596107153226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA902720.2114057119213576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1646500.082.317238
AGTGGTA1686600.080.613075
GTGGTAT1688550.080.4697956
GTATCAA1680050.080.469249
TGGTATC1685100.080.4316257
AAGCAGT1802100.076.031881
GCAGTGG1838150.074.364783
CAGTGGT1855050.073.50884
AGCAGTG1965850.069.787452
CAACGCA1583150.044.4871912-13
ATCAACG1608650.043.87947510-11
ACGCAGA1641050.042.98262814-15
TCAACGC1579650.042.7059612-13
AGAGTAC1672500.041.8263818-19
AACGCAG1610000.041.76436214-15
GAGTACT1601050.040.39363520-21
CGCAGAG1664250.040.3188116-17
TATCAAC1692000.039.97405610-11
AGTACTT1729050.039.381820-21
GTACTTT1693650.038.0636422-23
Produced by FastQC (version 0.11.5)