##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660662_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42700833 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.78467396643058 34.0 31.0 34.0 31.0 34.0 2 32.95819664688977 34.0 31.0 34.0 31.0 34.0 3 32.962626349701424 34.0 31.0 34.0 31.0 34.0 4 36.35625211339554 37.0 37.0 37.0 35.0 37.0 5 36.2828049279507 37.0 37.0 37.0 35.0 37.0 6 36.28069546090588 37.0 37.0 37.0 35.0 37.0 7 36.245702981953535 37.0 37.0 37.0 35.0 37.0 8 36.260275180111826 37.0 37.0 37.0 35.0 37.0 9 38.04605144353975 39.0 39.0 39.0 35.0 39.0 10-11 37.99644709507189 39.0 38.0 39.0 35.0 39.0 12-13 38.031485334255656 39.0 38.0 39.0 35.0 39.0 14-15 39.56612060706169 41.0 40.0 41.0 37.0 41.0 16-17 39.53078339244576 41.0 40.0 41.0 37.0 41.0 18-19 39.51225440965051 41.0 39.5 41.0 36.5 41.0 20-21 39.48884513564408 41.0 39.5 41.0 36.5 41.0 22-23 39.39527804996216 41.0 39.0 41.0 36.0 41.0 24-25 39.39137252896214 41.0 39.0 41.0 36.0 41.0 26-27 39.34465563002014 41.0 39.0 41.0 36.0 41.0 28-29 39.268708188901144 41.0 39.0 41.0 36.0 41.0 30-31 39.21167403221384 41.0 39.0 41.0 36.0 41.0 32-33 39.12982243226965 41.0 39.0 41.0 35.5 41.0 34-35 39.060268262213995 40.5 39.0 41.0 35.0 41.0 36-37 38.95267745713532 40.0 39.0 41.0 35.0 41.0 38-39 38.70190647100491 40.0 38.0 41.0 35.0 41.0 40-41 38.61505265014385 40.0 38.0 41.0 35.0 41.0 42-43 38.529432388356454 40.0 38.0 41.0 35.0 41.0 44-45 38.431197805906976 40.0 38.0 41.0 34.5 41.0 46-47 38.36063418247602 40.0 38.0 41.0 34.0 41.0 48-49 38.31064226545651 40.0 38.0 41.0 34.0 41.0 50-51 38.244692509862745 40.0 37.5 41.0 34.0 41.0 52-53 38.06319156584135 40.0 37.0 41.0 33.5 41.0 54-55 37.6559615827635 40.0 37.0 41.0 33.0 41.0 56-57 37.35689685491616 40.0 36.5 41.0 32.5 41.0 58-59 37.13515349923033 39.5 36.0 41.0 32.0 41.0 60-61 36.87900383582681 39.0 35.0 41.0 31.5 41.0 62-63 36.672554572881516 39.0 35.0 41.0 31.0 41.0 64-65 36.393632929830666 38.5 35.0 40.5 31.0 41.0 66-67 36.121494842969454 37.5 35.0 40.0 31.0 41.0 68-69 35.818833522521686 37.0 35.0 40.0 31.0 41.0 70-71 35.500049659452785 36.5 35.0 39.0 30.5 41.0 72-73 35.1530928448164 36.0 35.0 39.0 30.0 41.0 74-75 34.78896326214526 35.5 34.5 38.5 30.0 40.0 76-77 33.71557611065808 35.0 33.0 37.0 28.5 39.0 78-79 34.18962104322414 35.0 34.0 37.0 30.0 39.0 80-81 33.98936945796818 35.0 34.0 36.5 30.0 39.0 82-83 33.755208897212846 35.0 34.0 36.0 29.5 37.5 84-85 33.53397612875608 35.0 34.0 36.0 29.0 37.0 86-87 33.342564019769824 35.0 34.0 35.0 29.0 37.0 88-89 33.18196554854094 35.0 34.0 35.0 29.0 36.0 90-91 33.04390751580888 35.0 34.0 35.0 29.0 36.0 92-93 32.91520919509931 35.0 34.0 35.0 29.0 36.0 94-95 32.82791569897477 35.0 34.0 35.0 29.0 35.5 96-97 32.73835335952346 35.0 34.0 35.0 29.0 35.0 98-99 32.65255423705669 35.0 34.0 35.0 28.0 35.0 100 32.62774728539839 35.0 34.0 35.0 28.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05879026985967073 1101 2 0.05065631632613332 1101 3 0.04260870663593863 1101 4 0.039427067982920505 1101 5 0.026403596024870524 1101 6 0.03952471970676186 1101 7 0.044742909131478825 1101 8 0.053877178390287384 1101 9 0.05128016466666452 1101 10-11 0.053798424319445814 1101 12-13 0.04218905639967119 1101 14-15 0.03948310732172189 1101 16-17 0.05545023995913567 1101 18-19 0.047773624598200115 1101 20-21 0.0513638456181269 1101 22-23 0.06611174926323571 1101 24-25 0.0692343851252133 1101 26-27 0.07832496129348243 1101 28-29 0.11336220491971716 1101 30-31 0.10852164662762931 1101 32-33 0.11500180556132733 1101 34-35 0.14894811316826662 1101 36-37 0.1312277261653776 1101 38-39 0.12731425599486101 1101 40-41 0.10893576710285657 1101 42-43 0.13145576976856432 1101 44-45 0.1298415515738114 1101 46-47 0.10457787956885767 1101 48-49 0.1005994605455669 1101 50-51 0.06596951067992052 1101 52-53 0.036491636866770705 1101 54-55 0.07227939310561737 1101 56-57 0.040146355592604266 1101 58-59 -0.009828150712003492 1101 60-61 0.02870922566959422 1101 62-63 -1.5595611895946604E-4 1101 64-65 0.02658224119478092 1101 66-67 -0.019337606242267213 1101 68-69 0.014542188080149288 1101 70-71 0.01656274462513352 1101 72-73 0.022798135113369256 1101 74-75 0.039339665464993345 1101 76-77 0.2875043658233025 1101 78-79 0.06234783294398483 1101 80-81 0.06590733238981983 1101 82-83 0.043384605892129 1101 84-85 0.01454644766648272 1101 86-87 0.03410509209216883 1101 88-89 0.05104218132122895 1101 90-91 0.035128277091921234 1101 92-93 0.05311069417010117 1101 94-95 0.052549783592603205 1101 96-97 0.032825826805279235 1101 98-99 0.027880548055513543 1101 100 0.009323417429648373 1102 1 0.035759207986743036 1102 2 0.030282976421680985 1102 3 0.03065044916949944 1102 4 0.02418178588544606 1102 5 0.014444154871462445 1102 6 0.01765401040788106 1102 7 0.0376998680125169 1102 8 0.010579136071036999 1102 9 0.031092211147992543 1102 10-11 0.03708537577465165 1102 12-13 0.009538622374314798 1102 14-15 0.013574699723442052 1102 16-17 0.0543302229659659 1102 18-19 0.045415531640287554 1102 20-21 0.045104730479451405 1102 22-23 0.04219137825798214 1102 24-25 0.04352690064575171 1102 26-27 0.03872641945954314 1102 28-29 0.06565134999733147 1102 30-31 0.06969630218007694 1102 32-33 0.055537659545137785 1102 34-35 0.0752091689879748 1102 36-37 0.07132577622182623 1102 38-39 0.10380947223038817 1102 40-41 0.1124512136918625 1102 42-43 0.09538565064146098 1102 44-45 0.10821535173722907 1102 46-47 0.14058573124054163 1102 48-49 0.06971094658424448 1102 50-51 0.013562249802966164 1102 52-53 0.06611464604888084 1102 54-55 0.1038090405392893 1102 56-57 0.08032907185531712 1102 58-59 0.018157323167777406 1102 60-61 0.061403755737543975 1102 62-63 -0.002049594013364242 1102 64-65 0.01048207855740202 1102 66-67 0.0032934946737555038 1102 68-69 0.015560502258551878 1102 70-71 0.01420815828934252 1102 72-73 0.032291175278437834 1102 74-75 0.03814792162042835 1102 76-77 0.2623367860148633 1102 78-79 0.09569110426527061 1102 80-81 0.07319870133783724 1102 82-83 0.04461133044704013 1102 84-85 0.012223969681194546 1102 86-87 0.021542578590775463 1102 88-89 0.008932819938699765 1102 90-91 -0.0015789578396621096 1102 92-93 0.02847345796620715 1102 94-95 0.03197772567445867 1102 96-97 0.014682660869027586 1102 98-99 0.021263826137804642 1102 100 0.0024307947791086804 1103 1 -0.040584472380167824 1103 2 -0.046977832662939534 1103 3 -0.047983711307168164 1103 4 -0.026992850349714104 1103 5 -0.014570727712793996 1103 6 -0.009037568923552897 1103 7 -0.033575984658334335 1103 8 -0.024955648313209622 1103 9 -0.03555045727213724 1103 10-11 -0.04808886135834456 1103 12-13 -0.03423488002690789 1103 14-15 -0.031431685539793364 1103 16-17 -0.04275810302220151 1103 18-19 -0.045904106217790286 1103 20-21 -0.021863050528267536 1103 22-23 -0.018844010453783255 1103 24-25 -0.0076203308618687515 1103 26-27 -0.03640484832403246 1103 28-29 0.016072338869676628 1103 30-31 0.009252865792319653 1103 32-33 -0.03013645704598389 1103 34-35 -0.0015157790926352277 1103 36-37 0.025868198758573158 1103 38-39 0.07276494830742308 1103 40-41 0.06982405965778327 1103 42-43 0.06100291314873374 1103 44-45 0.07236202615745668 1103 46-47 0.06046535273025455 1103 48-49 0.056222020654715266 1103 50-51 0.001814225301941974 1103 52-53 -0.014787876784900789 1103 54-55 0.008991636171614914 1103 56-57 -0.0072245464512192825 1103 58-59 -0.05384689101190787 1103 60-61 -0.0022244283837764556 1103 62-63 -0.036896897468380985 1103 64-65 -0.023568193968799278 1103 66-67 -0.04350509851722251 1103 68-69 -0.04743351732548007 1103 70-71 -0.06799285923728604 1103 72-73 -0.048899608281608664 1103 74-75 -0.049414298388839484 1103 76-77 0.21290114659559123 1103 78-79 0.025832888898577266 1103 80-81 0.018283553322810064 1103 82-83 -0.019669216354394337 1103 84-85 -0.017067637483229703 1103 86-87 -0.005654736962320328 1103 88-89 -0.007640791056445551 1103 90-91 -0.011592754394023075 1103 92-93 -0.00471221997122484 1103 94-95 -0.013965082638414117 1103 96-97 -0.035364239243527607 1103 98-99 9.998945310840668E-4 1103 100 -0.050700008136054464 1104 1 -0.043554894181866644 1104 2 -0.039741481375259013 1104 3 -0.05435146287210557 1104 4 -0.02921866273404561 1104 5 -0.03276744422240796 1104 6 -0.02709516966024239 1104 7 -0.01799869041508373 1104 8 -0.031219546169261037 1104 9 -0.03087520966263213 1104 10-11 -0.03769945588135215 1104 12-13 -0.053956400929358495 1104 14-15 -0.073772221833039 1104 16-17 -0.050699851885553926 1104 18-19 -0.04724581781947279 1104 20-21 -0.05049507898736749 1104 22-23 -0.026030833225938466 1104 24-25 -0.044912923493932055 1104 26-27 -0.023213691695907812 1104 28-29 -0.005995069475090986 1104 30-31 -0.01774164846084858 1104 32-33 -0.010907468570323431 1104 34-35 -0.011139964824771198 1104 36-37 -0.00830877473171654 1104 38-39 0.0053342824498869845 1104 40-41 0.02886387917882871 1104 42-43 -0.013697908519880286 1104 44-45 -0.02209482365816484 1104 46-47 0.007990051065497994 1104 48-49 -0.01377644593497962 1104 50-51 -0.06666447589039137 1104 52-53 -0.10375500273219984 1104 54-55 -0.04120024572291925 1104 56-57 -0.04560307229233018 1104 58-59 -0.0835489227179309 1104 60-61 -0.07141185720205812 1104 62-63 -0.08051297144280056 1104 64-65 -0.05453704294964723 1104 66-67 -0.07775631631727009 1104 68-69 -0.06613746222139838 1104 70-71 -0.07823960989211542 1104 72-73 -0.06256949128856348 1104 74-75 -0.07540212033261895 1104 76-77 0.1202889326007508 1104 78-79 -0.0200075532469981 1104 80-81 -0.008024557423468082 1104 82-83 -0.003942515107731026 1104 84-85 -0.03335175534152057 1104 86-87 -0.05767679679521365 1104 88-89 -0.04827778461383758 1104 90-91 -0.0368985866468563 1104 92-93 -0.05245391303269997 1104 94-95 -0.04012614958414673 1104 96-97 -0.016496354949097736 1104 98-99 -0.016118276428748857 1104 100 -0.06819002703581845 1105 1 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-0.12463440990012487 1105 58-59 -0.1480623545602171 1105 60-61 -0.1067143601912548 1105 62-63 -0.11853517877693065 1105 64-65 -0.10795586557202341 1105 66-67 -0.1215597224067082 1105 68-69 -0.14044015028071755 1105 70-71 -0.16106770553998473 1105 72-73 -0.14058561835565087 1105 74-75 -0.13088037493641025 1105 76-77 0.17016054726099838 1105 78-79 -0.041824191762557916 1105 80-81 -0.04843417979655129 1105 82-83 -0.048093914499276025 1105 84-85 -0.093524281297384 1105 86-87 -0.09417998381572801 1105 88-89 -0.07899746765506421 1105 90-91 -0.08865517303740944 1105 92-93 -0.06574553272670158 1105 94-95 -0.053291374985114714 1105 96-97 -0.07245944701001861 1105 98-99 -0.07447765216149094 1105 100 -0.04430112296940081 1106 1 -0.1200977696415535 1106 2 -0.1030391079343218 1106 3 -0.12759955601445228 1106 4 -0.07122885349381392 1106 5 -0.08219958881539924 1106 6 -0.0814135031503298 1106 7 -0.05270134157989759 1106 8 -0.05640479873344617 1106 9 -0.10679500877768788 1106 10-11 -0.09818537815010586 1106 12-13 -0.09007675007168103 1106 14-15 -0.13729397183535497 1106 16-17 -0.1327010968801332 1106 18-19 -0.11901956706933703 1106 20-21 -0.10277433457535778 1106 22-23 -0.08966090455903242 1106 24-25 -0.09872835595278673 1106 26-27 -0.10265297873356616 1106 28-29 -0.12501142914010188 1106 30-31 -0.10667571089573613 1106 32-33 -0.09294486892991927 1106 34-35 -0.0803039703202657 1106 36-37 -0.07393901225887589 1106 38-39 -0.04881874652859608 1106 40-41 -0.0494987500832238 1106 42-43 -0.056011408786162065 1106 44-45 -0.05027483941280764 1106 46-47 -0.06049713056093253 1106 48-49 -0.07707337584018603 1106 50-51 -0.1268958726638516 1106 52-53 -0.12070010653850005 1106 54-55 -0.06515980044182612 1106 56-57 -0.09618949199165883 1106 58-59 -0.11690738550088042 1106 60-61 -0.13570733970038162 1106 62-63 -0.1668144033945822 1106 64-65 -0.13639544595931596 1106 66-67 -0.16688140162845144 1106 68-69 -0.1716467661963037 1106 70-71 -0.16226234179369925 1106 72-73 -0.1422421204987856 1106 74-75 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1107 30-31 -0.16476218723294522 1107 32-33 -0.1702659748621116 1107 34-35 -0.16655171889826192 1107 36-37 -0.1730529557172389 1107 38-39 -0.12506343666533581 1107 40-41 -0.12915192839730594 1107 42-43 -0.13014992790450464 1107 44-45 -0.1494951761699923 1107 46-47 -0.16746932439651374 1107 48-49 -0.18569416475457245 1107 50-51 -0.22967420907096425 1107 52-53 -0.2065508421162363 1107 54-55 -0.18138312834137338 1107 56-57 -0.19969029267200256 1107 58-59 -0.24341425944125916 1107 60-61 -0.21658836770054535 1107 62-63 -0.22866217703078462 1107 64-65 -0.2018884685322817 1107 66-67 -0.23805242447105712 1107 68-69 -0.2427171362713949 1107 70-71 -0.2184526585091291 1107 72-73 -0.19199687802446164 1107 74-75 -0.18404110136955154 1107 76-77 0.01906800546518639 1107 78-79 -0.13236836146718645 1107 80-81 -0.11100006006684993 1107 82-83 -0.12390443173568144 1107 84-85 -0.13432989781132676 1107 86-87 -0.11922823471411448 1107 88-89 -0.12902361562121456 1107 90-91 -0.13618750603158247 1107 92-93 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2315 64-65 0.5423863605970425 2315 66-67 0.5215531989700892 2315 68-69 0.49521321122475825 2315 70-71 0.49425231651606083 2315 72-73 0.4500300754288702 2315 74-75 0.47724555683841885 2315 76-77 -0.4395249124028737 2315 78-79 0.15635816986720386 2315 80-81 0.31202819242313495 2315 82-83 0.3374088533548516 2315 84-85 0.35037057125793325 2315 86-87 0.3251361796969974 2315 88-89 0.2950874990334782 2315 90-91 0.30729511541025545 2315 92-93 0.3496276179796638 2315 94-95 0.3297875811419644 2315 96-97 0.34272279179184295 2315 98-99 0.38981114940360584 2315 100 0.3720600959596325 2316 1 0.2702587394300977 2316 2 0.17807952124648097 2316 3 0.25748274898401746 2316 4 0.174753352098044 2316 5 0.21908959347525325 2316 6 0.21723222669219666 2316 7 0.23344342430497989 2316 8 0.2422347034498884 2316 9 0.32077361682937067 2316 10-11 0.31838224824158345 2316 12-13 0.3145831181793568 2316 14-15 0.37506915847115607 2316 16-17 0.3834318223691753 2316 18-19 0.3510528655522762 2316 20-21 0.3426760513620266 2316 22-23 0.17057385445784945 2316 24-25 -0.03582989471190956 2316 26-27 0.22286647645333346 2316 28-29 0.2938898579484359 2316 30-31 0.33856510512995897 2316 32-33 0.30066625401519786 2316 34-35 0.303168551606241 2316 36-37 0.2969110701280684 2316 38-39 0.09621250535371217 2316 40-41 0.08909172062013937 2316 42-43 0.2181694227005906 2316 44-45 0.22113231591674776 2316 46-47 0.23927859689138842 2316 48-49 0.04174831862654571 2316 50-51 0.33689470332318194 2316 52-53 0.4006142012371541 2316 54-55 0.4986224136834636 2316 56-57 0.141244379085542 2316 58-59 0.3523136192555185 2316 60-61 0.2446211872105195 2316 62-63 0.37975078073891666 2316 64-65 0.4207194557961742 2316 66-67 0.43960042040839653 2316 68-69 0.41221148911787253 2316 70-71 0.39447217013502467 2316 72-73 0.09213981630581003 2316 74-75 0.2934367254706203 2316 76-77 -0.18269499125955946 2316 78-79 0.23078549385698466 2316 80-81 0.1392388745007196 2316 82-83 0.047421960607501035 2316 84-85 -0.09255946910555224 2316 86-87 0.1315793912910479 2316 88-89 0.15866660117082176 2316 90-91 0.1752308229850712 2316 92-93 0.25427869466631847 2316 94-95 0.2410324836523543 2316 96-97 0.2593462705421956 2316 98-99 0.30330216645617725 2316 100 0.2499787097952293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 64.0 8 138.0 9 137.0 10 95.0 11 96.0 12 205.0 13 640.0 14 2471.0 15 7752.0 16 15769.0 17 23271.0 18 31530.0 19 39545.0 20 51893.0 21 72845.0 22 107821.0 23 173286.0 24 283828.0 25 397477.0 26 446637.0 27 422543.0 28 407228.0 29 447944.0 30 530278.0 31 643084.0 32 793027.0 33 1011525.0 34 1394257.0 35 2263002.0 36 4156297.0 37 9846479.0 38 1.4782211E7 39 4339029.0 40 8424.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.405714688544187 31.97998188497503 27.396713703485805 22.217589722994983 2 21.6801715258271 30.369902057703126 27.670924003670333 20.279002412799446 3 23.19240929881791 26.61430262426492 27.264341193796337 22.928946883120826 4 20.4362408445148 27.468786954343273 26.882920209707173 25.21205199143476 5 21.642828198937064 29.990973705622054 27.013645481732297 21.352552613708582 6 24.847003021297468 26.67500649187382 27.11686843622844 21.36112205060027 7 21.547069621500583 26.924875843168582 30.060470484509526 21.467584050821305 8 24.441108678137496 26.738654021105397 27.09427940199668 21.725957898760427 9 24.355396064521738 26.365206973831167 27.552097168689894 21.7272997929572 10-11 21.236348012227303 28.65474661817487 28.642933031306438 21.465972338291387 12-13 21.253535030569545 27.004549302352014 28.516969446474267 23.224946220604174 14-15 21.257136833841155 30.34032146398643 26.853911725797015 21.548629976375402 16-17 23.06776076241885 26.847715125370037 26.86532368115629 23.219200431054823 18-19 21.411874606533612 28.531420098613136 26.84073183237387 23.21597346247938 20-21 23.101092841466738 28.506875829454163 26.819655198118074 21.572376130961025 22-23 23.0470494293952 26.898066698737246 28.503793042692465 21.55109082917509 24-25 21.482990693523167 28.413799686593666 26.73259949765901 23.37061012222416 26-27 21.533270398844103 26.79138397223113 30.084027075336294 21.591318553588472 28-29 21.61859014032212 26.843365108658553 30.001134517974016 21.536910233045308 30-31 21.637089388811468 26.802274244358305 29.962542890936984 21.598093475893243 32-33 21.648599415747416 26.858423853057374 29.89299125260639 21.599985478588824 34-35 21.65981288587614 26.92672445398092 29.861506045587777 21.551956614555166 36-37 21.669950341522952 26.90640959808729 29.83144549356617 21.59219456682359 38-39 21.718625129221923 26.91716344103397 29.81113722189699 21.553074207847114 40-41 21.734693152130244 27.017152628749535 29.770632149478427 21.477522069641793 42-43 21.79304250410588 27.013260334683853 29.6892214651869 21.504475696023366 44-45 21.814489901090816 27.091303201184658 29.629369713225184 21.46483718449934 46-47 21.868004831030014 27.209117445978787 29.515910619901376 21.406967103089826 48-49 21.94132907699577 27.232508385644135 29.376527781859487 21.44963475550061 50-51 21.96739597949359 27.310543287293743 29.272377116242765 21.44968361696991 52-53 22.05405955064539 27.468251936619687 29.014044510067123 21.4636440026678 54-55 22.284284026724727 27.651760726013443 28.480206375887292 21.583748871374546 56-57 22.50132383499591 27.969349359293854 27.850260448337536 21.679066357372694 58-59 22.554035108696173 28.213024890020044 27.554985058485382 21.6779549427984 60-61 22.595225194317635 28.287930572279897 27.37858048817649 21.738263745225982 62-63 22.528816063597425 28.361006071779606 27.323660698884815 21.786517165738154 64-65 22.575609547672432 28.43204378933331 27.203029139518975 21.78931752347528 66-67 22.518389980513813 28.383347448319597 27.1800977252986 21.91816484586799 68-69 22.461286360410725 28.324129760353074 27.216502302759448 21.998081576476757 70-71 22.45224175630828 28.299940686024076 27.185956809569532 22.061860748098105 72-73 22.435298753712136 28.247397647532296 27.076833785332227 22.240469813423346 74-75 22.422462634738466 28.273257989346277 26.95551673139548 22.348762644519777 76-77 22.449967528905503 28.335965982888183 26.74078445700471 22.473282031201595 78-79 22.482601135956255 28.38720476879375 26.46368582318186 22.666508272068135 80-81 22.50454163186844 28.42010181728625 26.262416396285502 22.812940154559808 82-83 22.491702671774934 28.452659614567068 26.078318947097916 22.977318766560085 84-85 22.458472710019073 28.40669855871046 25.935185969624087 23.199642761646384 86-87 22.453446584705823 28.330681995130615 25.85157090002789 23.364300520135668 88-89 22.45001433009734 28.264236456395263 25.76867190137933 23.51707731212807 90-91 22.45616488308766 28.125489965013028 25.702312556408547 23.716032595490763 92-93 22.47290928458357 28.008184444613843 25.664669383964107 23.854236886838486 94-95 22.43400219595236 27.93903651984209 25.663927110690793 23.963034173514757 96-97 22.40063274478872 27.808073737320747 25.64472263023985 24.146570887650682 98-99 22.35591267562414 27.73397513244296 25.64296020951765 24.267151982415246 100 22.332452804816693 27.73765060453286 25.60403813623595 24.325858454414494 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 175544.0 1 103347.5 2 21455.5 3 10175.0 4 8021.0 5 7315.5 6 7195.5 7 7027.5 8 6898.0 9 6973.5 10 7533.5 11 12757.5 12 135765.0 13 155328.0 14 51889.5 15 49699.5 16 56037.0 17 63140.0 18 70865.5 19 80703.5 20 94409.5 21 116434.0 22 146478.0 23 180409.0 24 217868.0 25 260315.0 26 306359.0 27 361455.0 28 435877.0 29 512306.0 30 576550.5 31 645457.0 32 718601.5 33 780774.5 34 838011.5 35 910732.0 36 994020.0 37 1056148.0 38 1118303.0 39 1183091.0 40 1226736.0 41 1279158.5 42 1348690.0 43 1386292.0 44 1399518.0 45 1430556.0 46 1466383.5 47 1493244.0 48 1511634.0 49 1527648.5 50 1542527.0 51 1553169.5 52 1556257.0 53 1534108.0 54 1476292.0 55 1393710.0 56 1292776.0 57 1172128.5 58 1030768.0 59 869559.0 60 702111.0 61 553615.0 62 426339.0 63 314737.0 64 226678.0 65 161530.5 66 113589.0 67 81251.0 68 58252.5 69 42301.5 70 31682.0 71 24427.0 72 19271.0 73 14304.0 74 10932.0 75 9587.5 76 7120.5 77 3637.5 78 1862.5 79 1222.0 80 697.5 81 417.0 82 243.0 83 125.5 84 84.0 85 64.0 86 43.0 87 20.0 88 9.5 89 8.0 90 5.5 91 2.5 92 1.5 93 2.0 94 2.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07325618214520546 2 0.0422661543862622 3 0.03346304743047987 4 7.915536448668344E-4 5 7.49399900465642E-5 6 0.003037411471574805 7 2.3418746889551313E-6 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 5.503405519044558E-4 20-21 7.30664902954001E-4 22-23 5.140414942256513E-4 24-25 8.337073892680267E-4 26-27 0.00825276640387788 28-29 0.010037274916861691 30-31 0.012489217716197716 32-33 0.012313577114526079 34-35 0.015256142661198202 36-37 0.020671727879406944 38-39 0.017148377409873947 40-41 0.021576862446688102 42-43 0.024696239532376336 44-45 0.021179914686910207 46-47 0.021816904602306002 48-49 0.032183212912965886 50-51 0.011983372783383406 52-53 0.025725493458172113 54-55 0.06379969215120464 56-57 0.05989344517002748 58-59 0.05213364338817466 60-61 0.0754727665383015 62-63 0.040323569331773924 64-65 0.026580277719640735 66-67 0.03263051097855632 68-69 0.027200874512213848 70-71 0.014062957507175563 72-73 0.005505747393733514 74-75 0.004797330300324586 76-77 0.0048886634131938365 78-79 0.003666204825559258 80-81 0.003977674159190291 82-83 0.0015034835503091942 84-85 0.002460139360747365 86-87 0.0023699771852225926 88-89 0.0011592279710327899 90-91 1.873499751164105E-4 92-93 7.447161510877317E-4 94-95 0.0021275931549157366 96-97 0.002700181516365266 98-99 0.0015444663573659092 100 8.009211436226548E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.2700833E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.739805647915546 #Duplication Level Percentage of deduplicated Percentage of total 1 75.97348255691162 33.23065361434616 2 11.365339193001663 9.942354548490602 3 4.162361807446332 5.46182689482027 4 2.2632871152606273 3.9598295418772507 5 1.4135993508510116 3.0915280435121413 6 0.918130338915386 2.4095305550168287 7 0.6561271669320574 2.008921233334794 8 0.48232765503078767 1.6877534311729205 9 0.37050110346128645 1.4585081631961427 >10 2.0703318979726104 16.431733764259437 >50 0.19804020070136505 5.9780733942524655 >100 0.12500904563124038 10.29953865285045 >500 0.0010734773064921866 0.29106755370771176 >1k 3.301223188299867E-4 0.2541622513198738 >5k 3.752526620547764E-5 0.09420632842376672 >10k+ 2.144299249218992E-5 3.4003120294193776 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1215017 2.8454175589501967 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 120271 0.2816596107153226 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 90272 0.2114057119213576 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.012643781445668753 1.6393122822685918E-5 0.0 2.3418746889551313E-6 7.025624066865393E-6 2 0.012653148944424574 2.107687220059618E-5 0.0 2.3418746889551313E-6 1.1709373444775655E-5 3 0.012655490819113529 2.5760621578506443E-5 0.0 2.3418746889551313E-6 1.6393122822685918E-5 4 0.01266017456849144 3.0444370956416706E-5 2.3418746889551313E-6 4.683749377910263E-6 2.5760621578506443E-5 5 0.01266485831786935 3.981186971223723E-5 2.3418746889551313E-6 7.025624066865393E-6 3.0444370956416706E-5 6 0.012676567691314125 4.4495619090147494E-5 2.3418746889551313E-6 7.025624066865393E-6 3.74699950232821E-5 7 0.01267890956600308 4.917936846805775E-5 2.3418746889551313E-6 7.025624066865393E-6 4.215374440119236E-5 8 0.012681251440692036 4.917936846805775E-5 2.3418746889551313E-6 1.1709373444775655E-5 4.683749377910262E-5 9 0.012688277064758901 4.917936846805775E-5 2.3418746889551313E-6 1.4051248133730786E-5 4.917936846805775E-5 10-11 0.012703499250237109 5.854686722387828E-5 2.3418746889551313E-6 1.6393122822685918E-5 4.917936846805775E-5 12-13 0.012722234247748751 6.44015539462661E-5 2.3418746889551313E-6 1.6393122822685918E-5 5.269218050149045E-5 14-15 0.012738627370571437 6.908530332417637E-5 2.3418746889551313E-6 1.9905934856118617E-5 5.854686722387828E-5 16-17 0.012752678618705167 7.49399900465642E-5 2.3418746889551313E-6 2.107687220059618E-5 6.557249129074367E-5 18-19 0.01278429392700606 7.845280207999689E-5 2.3418746889551313E-6 2.5760621578506443E-5 7.611092739104176E-5 20-21 0.012820592984684865 9.367498755820524E-5 2.3418746889551313E-6 2.5760621578506443E-5 9.367498755820524E-5 22-23 0.012964618278055607 1.053843610029809E-4 2.3418746889551313E-6 3.2786245645371836E-5 1.1240998506984629E-4 24-25 0.013612146629551701 1.1475185975880142E-4 2.3418746889551313E-6 3.39571829898494E-5 1.2177748382566681E-4 26-27 0.016709275905694862 1.2646123320357708E-4 2.3418746889551313E-6 3.6299057678804535E-5 1.288031078925322E-4 28-29 0.02712242171013385 1.288031078925322E-4 2.3418746889551313E-6 4.215374440119236E-5 1.393415439928303E-4 30-31 0.05270857362431314 1.3817060664835274E-4 2.3418746889551313E-6 4.5666556434625054E-5 1.4402529337074056E-4 32-33 0.10371109153772246 1.4753810540417327E-4 2.3418746889551313E-6 4.683749377910262E-5 1.5105091743760596E-4 34-35 0.1916449732959542 1.6041841619342646E-4 2.3418746889551313E-6 4.800843112358019E-5 1.6158935353790404E-4 36-37 0.32474776311740805 1.662731029158143E-4 2.3418746889551313E-6 5.1521243157012886E-5 1.662731029158143E-4 38-39 0.5085345758945733 1.7915341370506753E-4 2.3418746889551313E-6 5.269218050149045E-5 1.803243510495451E-4 40-41 0.7458800628081423 1.8734997511641048E-4 2.3418746889551313E-6 5.5034055190445586E-5 1.8969184980536561E-4 42-43 1.037898956210058 2.0491403528357397E-4 2.3418746889551313E-6 5.9717804568355845E-5 2.002302859056637E-4 44-45 1.3852118528928932 2.0842684731700668E-4 2.3418746889551313E-6 6.088874191283341E-5 2.0725590997252913E-4 46-47 1.7907308740323638 2.1311059669491694E-4 2.3418746889551313E-6 6.557249129074367E-5 2.2247809545073745E-4 48-49 2.2529455104540936 2.1779434607282718E-4 2.3418746889551313E-6 7.14271780131315E-5 2.306746568620804E-4 50-51 2.7670197909253904 2.2716184482864772E-4 2.3418746889551313E-6 7.611092739104176E-5 2.3770028092894583E-4 52-53 3.3305111401456733 2.4355496765133362E-4 2.3418746889551313E-6 7.962373942447446E-5 2.4940965437372147E-4 54-55 3.94235166325678 2.6111902781849716E-4 2.3418746889551313E-6 8.196561411342959E-5 2.552643410961093E-4 56-57 4.6003001861813795 2.6814465188536253E-4 2.3418746889551313E-6 8.196561411342959E-5 2.6814465188536253E-4 58-59 5.3005675556727425 2.833668373635709E-4 2.3418746889551313E-6 8.899123818029499E-5 2.751702759522279E-4 60-61 6.038909123857139 2.9273433611939144E-4 2.3418746889551313E-6 9.250405021372769E-5 2.8453777470804843E-4 62-63 6.810872986950864 2.962471481528241E-4 2.3418746889551313E-6 9.601686224716037E-5 2.915633987749138E-4 64-65 7.614753089243013 3.0912745894207734E-4 2.3418746889551313E-6 9.83587369361155E-5 2.962471481528241E-4 66-67 8.447234507111371 3.184949576978978E-4 2.3418746889551313E-6 1.0070061162507064E-4 3.056146469086446E-4 68-69 9.302309161041425 3.2552058176476325E-4 2.3418746889551313E-6 1.0070061162507064E-4 3.1381120831998756E-4 70-71 10.182263891666938 3.372299552095389E-4 2.3418746889551313E-6 1.053843610029809E-4 3.231787070758081E-4 72-73 11.081652669398744 3.501102659987921E-4 2.3418746889551313E-6 1.053843610029809E-4 3.2903339379819594E-4 74-75 11.998187482665736 3.5830682741013506E-4 2.3418746889551313E-6 1.0655529834745847E-4 3.39571829898494E-4 76-77 12.929781721120055 3.606487020990902E-4 2.3418746889551313E-6 1.1123904772536872E-4 3.47768391309837E-4 78-79 13.877123895920251 3.629905767880454E-4 2.3418746889551313E-6 1.229484211701444E-4 3.5713589006565753E-4 80-81 14.836523212556532 3.6884526351043317E-4 2.3418746889551313E-6 1.2646123320357708E-4 3.641615141325229E-4 82-83 15.80736024517367 3.7587088757729854E-4 2.3418746889551313E-6 1.2646123320357708E-4 3.770418249217761E-4 84-85 16.78792776712342 3.852383863331191E-4 2.3418746889551313E-6 1.288031078925322E-4 3.8289651164416397E-4 86-87 17.779308895449418 4.004605718113274E-4 2.3418746889551313E-6 1.3114498258148734E-4 3.910930730555069E-4 88 18.52415619151973 4.074861958781928E-4 2.3418746889551313E-6 1.3114498258148734E-4 4.0280244650028256E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 164650 0.0 82.31723 8 AGTGGTA 168660 0.0 80.61307 5 GTGGTAT 168855 0.0 80.469795 6 GTATCAA 168005 0.0 80.46924 9 TGGTATC 168510 0.0 80.431625 7 AAGCAGT 180210 0.0 76.03188 1 GCAGTGG 183815 0.0 74.36478 3 CAGTGGT 185505 0.0 73.5088 4 AGCAGTG 196585 0.0 69.78745 2 CAACGCA 158315 0.0 44.48719 12-13 ATCAACG 160865 0.0 43.879475 10-11 ACGCAGA 164105 0.0 42.982628 14-15 TCAACGC 157965 0.0 42.70596 12-13 AGAGTAC 167250 0.0 41.82638 18-19 AACGCAG 161000 0.0 41.764362 14-15 GAGTACT 160105 0.0 40.393635 20-21 CGCAGAG 166425 0.0 40.31881 16-17 TATCAAC 169200 0.0 39.974056 10-11 AGTACTT 172905 0.0 39.3818 20-21 GTACTTT 169365 0.0 38.06364 22-23 >>END_MODULE