FastQCFastQC Report
Thu 26 May 2016
SRR1660661_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660661_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40631418
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4885301.2023454362336061No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1617370.3980589601869174Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1359690.33464005612602543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA755500.074.1903848
GTATCAA786550.071.175029
GTGGTAT802500.070.106526
AGTGGTA805250.069.963855
TGGTATC803850.069.8250967
CAGTGGT933450.060.7121164
AAGCAGT939600.060.674941
GCAGTGG961250.059.1711853
AGCAGTG1104450.051.8184662
CAACGCA710700.041.51621612-13
ATCAACG731950.040.41758310-11
TCAACGC702550.039.77669512-13
ACGCAGA768750.038.62172314-15
AACGCAG734550.038.13817214-15
AGAGTAC793800.037.15382818-19
GAGTACT723800.036.53243320-21
CGCAGAG806450.034.80805216-17
TATCAAC818000.034.25527210-11
AGTACTT845300.033.04590220-21
GTACTTT818750.032.20039422-23
Produced by FastQC (version 0.11.5)