FastQCFastQC Report
Thu 26 May 2016
SRR1660661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40631418
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10234532.518870987963058No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCAATCTCGTATG1662450.4091538227880701TruSeq Adapter, Index 16 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT917150.22572433972154257No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA772820.19020256689047868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1414650.080.399728
GTATCAA1440600.078.719899
AGTGGTA1450450.078.5444265
GTGGTAT1453300.078.386786
TGGTATC1456600.078.042287
AAGCAGT1565150.073.522141
CAGTGGT1571200.072.7388464
GCAGTGG1589700.072.112963
AGCAGTG1720350.066.910692
CAACGCA1355650.043.89752212-13
ATCAACG1383450.043.09156410-11
ACGCAGA1417300.041.98013714-15
TCAACGC1351750.041.79437612-13
AGAGTAC1441900.040.97849318-19
AACGCAG1387300.040.6068814-15
GAGTACT1366300.039.68560820-21
AGTACTT1485900.038.7477120-21
CGCAGAG1459300.038.50218616-17
TATCAAC1473300.038.48315410-11
GTACTTT1463600.036.9572222-23
Produced by FastQC (version 0.11.5)