##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660661_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40631418 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.71371223125907 34.0 31.0 34.0 31.0 34.0 2 32.893183570408496 34.0 31.0 34.0 31.0 34.0 3 32.89644200948143 34.0 31.0 34.0 31.0 34.0 4 36.30904653635273 37.0 37.0 37.0 35.0 37.0 5 36.231107956901724 37.0 37.0 37.0 35.0 37.0 6 36.2241431495204 37.0 37.0 37.0 35.0 37.0 7 36.193296601167106 37.0 37.0 37.0 35.0 37.0 8 36.20262170028129 37.0 37.0 37.0 35.0 37.0 9 37.974983226034595 39.0 38.0 39.0 35.0 39.0 10-11 37.929935315080556 39.0 38.0 39.0 35.0 39.0 12-13 37.95781662111817 39.0 38.0 39.0 35.0 39.0 14-15 39.47423189857661 41.0 39.0 41.0 36.0 41.0 16-17 39.44256023749897 41.0 39.0 41.0 36.0 41.0 18-19 39.42528452981877 41.0 39.0 41.0 36.0 41.0 20-21 39.40651492891536 41.0 39.0 41.0 36.0 41.0 22-23 39.320369362939786 41.0 39.0 41.0 36.0 41.0 24-25 39.31271260333567 41.0 39.0 41.0 36.0 41.0 26-27 39.25558070604379 41.0 39.0 41.0 36.0 41.0 28-29 39.171351883411994 41.0 39.0 41.0 36.0 41.0 30-31 39.109843311400056 41.0 39.0 41.0 35.5 41.0 32-33 39.02962749171097 40.5 39.0 41.0 35.0 41.0 34-35 38.96258875336322 40.0 39.0 41.0 35.0 41.0 36-37 38.85828245029499 40.0 38.0 41.0 35.0 41.0 38-39 38.626725936564654 40.0 38.0 41.0 35.0 41.0 40-41 38.53420902022174 40.0 38.0 41.0 35.0 41.0 42-43 38.41447614503633 40.0 38.0 41.0 34.0 41.0 44-45 38.33189364693105 40.0 38.0 41.0 34.0 41.0 46-47 38.25789354681149 40.0 38.0 41.0 34.0 41.0 48-49 38.1991683258507 40.0 37.0 41.0 34.0 41.0 50-51 38.11886717810341 40.0 37.0 41.0 33.5 41.0 52-53 37.942004288405585 40.0 37.0 41.0 33.0 41.0 54-55 37.54993731697968 40.0 36.5 41.0 33.0 41.0 56-57 37.264299070241655 40.0 36.0 41.0 32.0 41.0 58-59 37.028590387861925 39.0 35.5 41.0 32.0 41.0 60-61 36.76688140689552 39.0 35.0 41.0 31.0 41.0 62-63 36.54526699018971 39.0 35.0 41.0 31.0 41.0 64-65 36.252218960214485 38.0 35.0 40.0 31.0 41.0 66-67 35.97035843051306 37.0 35.0 40.0 31.0 41.0 68-69 35.65490758899924 37.0 35.0 39.5 30.5 41.0 70-71 35.32046593845186 36.0 35.0 39.0 30.0 41.0 72-73 34.96319129201939 36.0 34.5 39.0 30.0 40.5 74-75 34.55905452278333 35.0 34.0 37.5 29.0 40.0 76-77 33.58174555955689 35.0 33.0 36.5 28.5 39.0 78-79 33.97487737198834 35.0 34.0 37.0 29.0 39.0 80-81 33.78149405221349 35.0 34.0 36.0 29.0 39.0 82-83 33.561861279367605 35.0 34.0 36.0 29.0 37.0 84-85 33.35808089444478 35.0 34.0 35.5 29.0 37.0 86-87 33.183351649208994 35.0 34.0 35.0 29.0 36.5 88-89 33.038677631186786 35.0 34.0 35.0 29.0 36.0 90-91 32.91174382592308 35.0 34.0 35.0 29.0 36.0 92-93 32.79626442030647 35.0 34.0 35.0 29.0 36.0 94-95 32.71534829279155 35.0 34.0 35.0 29.0 35.0 96-97 32.63075294344884 35.0 34.0 35.0 27.5 35.0 98-99 32.5604457245376 35.0 34.0 35.0 27.0 35.0 100 32.53530309968507 35.0 34.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.038977630466277446 1101 2 0.03186005235275502 1101 3 0.029729657696705658 1101 4 0.028539019831512746 1101 5 0.0057815777189063056 1101 6 0.03897482947497366 1101 7 0.04144253718968827 1101 8 0.02964595697187633 1101 9 0.025847370902972955 1101 10-11 0.05183759610760319 1101 12-13 0.029265001624622755 1101 14-15 0.042013092747460234 1101 16-17 0.045780615985151485 1101 18-19 0.0526276575484701 1101 20-21 0.06615959360532031 1101 22-23 0.07501349597367124 1101 24-25 0.07509711330256863 1101 26-27 0.057923282621828776 1101 28-29 0.10431964996468679 1101 30-31 0.0971054740412427 1101 32-33 0.10686274935157059 1101 34-35 0.12466005923659651 1101 36-37 0.10712717599017907 1101 38-39 0.13004110038717442 1101 40-41 0.11128920127045205 1101 42-43 0.11354017810975137 1101 44-45 0.1145349495111887 1101 46-47 0.056021426726594825 1101 48-49 0.09158521537332831 1101 50-51 0.06499544714942118 1101 52-53 0.024304286200035108 1101 54-55 0.0440317312684968 1101 56-57 0.007031424857963486 1101 58-59 0.002882849132660681 1101 60-61 0.015583974551077517 1101 62-63 -0.010737238648040659 1101 64-65 0.02833039440408669 1101 66-67 0.009508023585361514 1101 68-69 0.0330727261820698 1101 70-71 0.018681570673031445 1101 72-73 0.020014652929461363 1101 74-75 0.04500296265035786 1101 76-77 0.33059777497653897 1101 78-79 0.09114478183984431 1101 80-81 0.07460800716163618 1101 82-83 0.052612042495582045 1101 84-85 0.03323602182241814 1101 86-87 0.045286294676252226 1101 88-89 0.04695876278160682 1101 90-91 0.03416367960971911 1101 92-93 0.03603494598250023 1101 94-95 0.03051019901877794 1101 96-97 0.03441997361598226 1101 98-99 0.020817483938763814 1101 100 -0.012194798293535314 1102 1 0.02334688736618773 1102 2 0.018109624532840485 1102 3 -0.008649583920913528 1102 4 0.018165032796346736 1102 5 0.02104938986626337 1102 6 0.02706131471175155 1102 7 0.021942703273595043 1102 8 0.02585762427568028 1102 9 0.02683702846836411 1102 10-11 0.02233643459628354 1102 12-13 0.010397204590006481 1102 14-15 0.002566404898402652 1102 16-17 0.02379158118491631 1102 18-19 0.03352144999387008 1102 20-21 0.00880945447272552 1102 22-23 0.027783397200387583 1102 24-25 0.04351437311956374 1102 26-27 0.033954601090904646 1102 28-29 0.04708446297309621 1102 30-31 0.0734673410982083 1102 32-33 0.048713306875434625 1102 34-35 0.07338422171034154 1102 36-37 0.0909119114719843 1102 38-39 0.11578868729827008 1102 40-41 0.11380113916575141 1102 42-43 0.10107902984853467 1102 44-45 0.11348135808400883 1102 46-47 0.12048702296238645 1102 48-49 0.06969858329254208 1102 50-51 0.02736318427934492 1102 52-53 0.014491409630046803 1102 54-55 0.03980059174231343 1102 56-57 0.03707048017106729 1102 58-59 2.7495650132181026E-4 1102 60-61 0.01777651822525428 1102 62-63 -0.04176011863759754 1102 64-65 0.005887284448824914 1102 66-67 -0.023025752582604753 1102 68-69 -0.010393774445269344 1102 70-71 -0.015244734987469144 1102 72-73 0.006485581119761719 1102 74-75 -0.02286232746286032 1102 76-77 0.25962454892409426 1102 78-79 0.05951333196678377 1102 80-81 0.025837187834738984 1102 82-83 0.041905984315242506 1102 84-85 0.051866749214951824 1102 86-87 0.0044594151861403475 1102 88-89 -0.005211281512437438 1102 90-91 -0.004220171246501536 1102 92-93 0.010271058255504784 1102 94-95 -0.018922223757542156 1102 96-97 0.005380913378388641 1102 98-99 -0.01235597832638291 1102 100 -0.0471519186714886 1103 1 -0.03024107463429715 1103 2 -0.04317618864563144 1103 3 -0.050787147275279665 1103 4 -0.022116506952734483 1103 5 -0.028754226074319433 1103 6 -0.03915671417008326 1103 7 -0.05999592237738227 1103 8 -0.034988779285278326 1103 9 -0.04227225939339263 1103 10-11 -0.05297001660245826 1103 12-13 -0.04464520796238247 1103 14-15 -0.029641245588841514 1103 16-17 -0.044759287345705445 1103 18-19 -0.044909609127046224 1103 20-21 -0.039326081279476455 1103 22-23 -0.03461517512196366 1103 24-25 -0.030395934523959056 1103 26-27 -0.0790967170737531 1103 28-29 -0.01644523300853251 1103 30-31 -0.023267015029773574 1103 32-33 -0.04917944001716279 1103 34-35 -0.030719143534504667 1103 36-37 -0.027971940073740598 1103 38-39 0.020029902307562963 1103 40-41 0.048075853913076116 1103 42-43 0.013999692448464884 1103 44-45 0.02849310286911333 1103 46-47 0.027992925471288288 1103 48-49 0.006224723519245856 1103 50-51 -0.05862048122029506 1103 52-53 -0.0402269997482918 1103 54-55 -0.06306697257326022 1103 56-57 -0.07575464502647122 1103 58-59 -0.11442651393432612 1103 60-61 -0.04719108413929973 1103 62-63 -0.07418242343012338 1103 64-65 -0.05830757996971414 1103 66-67 -0.08648385161306038 1103 68-69 -0.0797424951089738 1103 70-71 -0.09678547527901316 1103 72-73 -0.039283941776375286 1103 74-75 -0.05300355932047296 1103 76-77 0.23809123386391207 1103 78-79 0.023689633707896007 1103 80-81 -0.01667251139733139 1103 82-83 -0.04420429728191522 1103 84-85 -0.03329044782475421 1103 86-87 -0.03539250431939678 1103 88-89 -0.03140342306340216 1103 90-91 -0.034781268055070313 1103 92-93 -0.01455601727703737 1103 94-95 -0.04303113230918143 1103 96-97 -0.025664739955530536 1103 98-99 -0.04028025166763882 1103 100 -0.06626792885070643 1104 1 -0.055156378492029035 1104 2 -0.0692686988823894 1104 3 -0.09684091914552084 1104 4 -0.04375423747698903 1104 5 -0.0557883098635088 1104 6 -0.04628050532357264 1104 7 -0.029551609364681042 1104 8 -0.039315351906246576 1104 9 -0.04964535198023157 1104 10-11 -0.049107827126434245 1104 12-13 -0.05029085568364877 1104 14-15 -0.09049953238142194 1104 16-17 -0.058615462913834904 1104 18-19 -0.050891948566572864 1104 20-21 -0.0549265093424296 1104 22-23 -0.04244832954432809 1104 24-25 -0.05707735747597553 1104 26-27 -0.03995908897207556 1104 28-29 -0.024176854102194056 1104 30-31 2.643737896690368E-4 1104 32-33 -0.029882144118431597 1104 34-35 -0.030615142245288496 1104 36-37 -0.007696672169295482 1104 38-39 -6.499049808823543E-4 1104 40-41 0.027742100228536515 1104 42-43 -0.012192942394221973 1104 44-45 -0.015935383492795552 1104 46-47 2.3468460420161819E-4 1104 48-49 -0.007926676195303628 1104 50-51 -0.0721198826837437 1104 52-53 -0.07233590169795434 1104 54-55 -0.009912033472247117 1104 56-57 -0.03544152435437553 1104 58-59 -0.10362736825383934 1104 60-61 -0.0760935941658829 1104 62-63 -0.09490393233755157 1104 64-65 -0.05341769457026402 1104 66-67 -0.075225871417409 1104 68-69 -0.09891227971486671 1104 70-71 -0.11471587878765632 1104 72-73 -0.0843916659247057 1104 74-75 -0.08567360039129568 1104 76-77 0.16953188149794585 1104 78-79 -0.007981656537850768 1104 80-81 -0.021944016052074744 1104 82-83 -0.029411862109732567 1104 84-85 -0.03209543483476551 1104 86-87 -0.06082495277845368 1104 88-89 -0.056407690840082125 1104 90-91 -0.061968104709016814 1104 92-93 -0.0457449427072234 1104 94-95 -0.049043051755440104 1104 96-97 -0.012317756460120677 1104 98-99 -0.006918754788486581 1104 100 -0.08606329228879872 1105 1 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-0.10747668949152 1105 58-59 -0.14735064177110502 1105 60-61 -0.1166712675754269 1105 62-63 -0.12889103749899533 1105 64-65 -0.11172482320112209 1105 66-67 -0.14731111663669338 1105 68-69 -0.13738247464559095 1105 70-71 -0.15887869937846943 1105 72-73 -0.13641048635763298 1105 74-75 -0.15103529404185423 1105 76-77 0.19432895877476852 1105 78-79 -0.024936141192220873 1105 80-81 -0.06421600910528014 1105 82-83 -0.07743997254687685 1105 84-85 -0.09574770671528654 1105 86-87 -0.10851073564632685 1105 88-89 -0.08096523604023531 1105 90-91 -0.09819462469808116 1105 92-93 -0.07163404465838852 1105 94-95 -0.07075177566780155 1105 96-97 -0.08764177954216734 1105 98-99 -0.0678270385943236 1105 100 -0.0796584413022714 1106 1 -0.1311483719101929 1106 2 -0.11517859737077174 1106 3 -0.14086532050792755 1106 4 -0.08625069218029324 1106 5 -0.10260883706552448 1106 6 -0.1158529060589899 1106 7 -0.08130337804055898 1106 8 -0.08252854025325007 1106 9 -0.10177365655664516 1106 10-11 -0.1373408875441413 1106 12-13 -0.12581390417725657 1106 14-15 -0.1594374871585913 1106 16-17 -0.1552295861636921 1106 18-19 -0.16439899184793205 1106 20-21 -0.1347533262988989 1106 22-23 -0.1289430045499529 1106 24-25 -0.12677898732259507 1106 26-27 -0.11965865614287452 1106 28-29 -0.14806865367372524 1106 30-31 -0.12076309021468745 1106 32-33 -0.11420861124749848 1106 34-35 -0.09171732963641688 1106 36-37 -0.08189475950515401 1106 38-39 -0.0802918839782123 1106 40-41 -0.08333814854762522 1106 42-43 -0.09769282204343455 1106 44-45 -0.09568545170576925 1106 46-47 -0.09408905140509205 1106 48-49 -0.12795117276038326 1106 50-51 -0.16647594943749056 1106 52-53 -0.16402767032454335 1106 54-55 -0.16302362755450162 1106 56-57 -0.17414900581704984 1106 58-59 -0.1921151919633246 1106 60-61 -0.20992154896105575 1106 62-63 -0.23897844822305103 1106 64-65 -0.21547856053931014 1106 66-67 -0.23875230095497812 1106 68-69 -0.25647144141758815 1106 70-71 -0.19960147144789175 1106 72-73 -0.20076604647261576 1106 74-75 -0.22740851896453762 1106 76-77 0.08823652893705969 1106 78-79 -0.11621758730670706 1106 80-81 -0.10519438228459421 1106 82-83 -0.1285745585687792 1106 84-85 -0.1647304195369017 1106 86-87 -0.15742313211624293 1106 88-89 -0.1712260870606528 1106 90-91 -0.17517485533529964 1106 92-93 -0.16894656597423108 1106 94-95 -0.13894599731820279 1106 96-97 -0.1545138989223247 1106 98-99 -0.18208268178905485 1106 100 -0.18174098610752765 1107 1 -0.162597983459996 1107 2 -0.13543834110943465 1107 3 -0.1643973724399359 1107 4 -0.09515301201439286 1107 5 -0.14378116335706181 1107 6 -0.1188660220733837 1107 7 -0.14056370377106475 1107 8 -0.13808331168512922 1107 9 -0.16049727922290913 1107 10-11 -0.16443068688357698 1107 12-13 -0.14176799279852048 1107 14-15 -0.20892356002072177 1107 16-17 -0.1645932851890919 1107 18-19 -0.17374669515557173 1107 20-21 -0.16616821992219144 1107 22-23 -0.1739477570182757 1107 24-25 -0.1697812670398804 1107 26-27 -0.1803749789745055 1107 28-29 -0.15477604339623952 1107 30-31 -0.19776689991896745 1107 32-33 -0.201741526179255 1107 34-35 -0.2001669145778564 1107 36-37 -0.19052989591246927 1107 38-39 -0.1608885697378284 1107 40-41 -0.1472777802437406 1107 42-43 -0.14922987001637722 1107 44-45 -0.15025290018699167 1107 46-47 -0.18924508050556454 1107 48-49 -0.20098506188530507 1107 50-51 -0.2717694816184846 1107 52-53 -0.244490910383945 1107 54-55 -0.1947423953616081 1107 56-57 -0.2146866668614038 1107 58-59 -0.2606370268794862 1107 60-61 -0.2518856465641761 1107 62-63 -0.2817540572212849 1107 64-65 -0.22495391689366073 1107 66-67 -0.27516816834893376 1107 68-69 -0.27110642664599993 1107 70-71 -0.2914735743726382 1107 72-73 -0.2514352906621369 1107 74-75 -0.25251431015844616 1107 76-77 0.013615363841616102 1107 78-79 -0.14652921258220886 1107 80-81 -0.13322547186255207 1107 82-83 -0.1540003659433964 1107 84-85 -0.16186312937635705 1107 86-87 -0.16061998915482434 1107 88-89 -0.13941673827746826 1107 90-91 -0.14216504959100718 1107 92-93 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0.3569750898262285 2316 38-39 0.13771422828111923 2316 40-41 0.17025046240182462 2316 42-43 0.2793368510928147 2316 44-45 0.27170109883991955 2316 46-47 0.2606758798946842 2316 48-49 0.12694823357576723 2316 50-51 0.43278535261743656 2316 52-53 0.47905228719447734 2316 54-55 0.5377510457038142 2316 56-57 0.2044849112490823 2316 58-59 0.4245991802848792 2316 60-61 0.2896086698477234 2316 62-63 0.4408375836511311 2316 64-65 0.49413691646026336 2316 66-67 0.4920532019685169 2316 68-69 0.4979514593697303 2316 70-71 0.45948092755816106 2316 72-73 0.14453162539120967 2316 74-75 0.3567736383099529 2316 76-77 -0.180306068929454 2316 78-79 0.25521772319171987 2316 80-81 0.18500582667935817 2316 82-83 0.08195926273887011 2316 84-85 -0.04791074445422794 2316 86-87 0.17309864229270033 2316 88-89 0.21418698204944775 2316 90-91 0.24483822929534682 2316 92-93 0.29417036278992725 2316 94-95 0.25461538785827287 2316 96-97 0.2811489164644314 2316 98-99 0.275587153827054 2316 100 0.29125326674758156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 32.0 8 127.0 9 125.0 10 98.0 11 103.0 12 184.0 13 707.0 14 2730.0 15 7759.0 16 17098.0 17 23251.0 18 28136.0 19 37786.0 20 51459.0 21 71693.0 22 105396.0 23 165276.0 24 265044.0 25 366051.0 26 418319.0 27 413610.0 28 419980.0 29 477333.0 30 608525.0 31 682666.0 32 809283.0 33 1032867.0 34 1414263.0 35 2260809.0 36 4191356.0 37 9609755.0 38 1.3523134E7 39 3620637.0 40 5824.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.321307507699153 31.678128496955853 26.59492465268477 23.405639342660223 2 22.50822584377158 29.261301231701342 27.94078377305855 20.289689151468533 3 22.793008800354897 26.971346810268365 26.68496362332529 23.550680766051453 4 20.482706983574573 27.346419950113987 27.012601158533382 25.15827190777806 5 22.106599770591114 29.366448820273945 26.562497923403143 21.96445348573179 6 25.20926260243342 26.38329020023357 26.819536279182525 21.58791091815048 7 22.324955530717634 26.802303577000437 29.20007369666498 21.67266719561695 8 24.073289787720427 26.778565788671223 26.979993659094053 22.168150764514298 9 24.102146767311936 26.336998625054136 27.479865457809026 22.080989149824898 10-11 21.78698759664258 28.29482101756823 28.078122944170936 21.840068441618257 12-13 21.854332034387774 26.650594621137763 27.87807331754949 23.617000026924977 14-15 21.61480310630557 29.80412965159129 26.41209076188284 22.168976480220305 16-17 23.221896907462103 26.720551569231475 26.426649692609793 23.63090183069663 18-19 22.00389205827182 27.962973921974076 26.611923514295377 23.42121050545873 20-21 23.285165765580356 27.949955776533287 26.600154959952764 22.164723497933593 22-23 23.447075723597937 26.78880071342165 27.837645381701577 21.92647818127883 24-25 21.87461401924318 28.07246158534305 26.273224112574827 23.779700282838945 26-27 21.912359991917427 26.420510628524752 29.468967516619728 22.198161862938097 28-29 21.985445589715066 26.71111718475419 29.17580266832086 22.127634557209884 30-31 22.210712713017745 26.433785150558837 29.362850084095747 21.992652052327667 32-33 22.021649915367636 26.711308846175562 29.06143044966288 22.20561078879392 34-35 22.04426136427902 26.57168500351735 29.012493135550205 22.371560496653426 36-37 22.0583427803895 26.546979037726615 29.210715319965956 22.183962861917927 38-39 22.119816881977904 26.56273492717116 29.194396790964383 22.12305139988655 40-41 22.138098294563278 27.12303478594245 28.94262573655294 21.79624118294133 42-43 22.186752061905395 26.685951789261388 29.050783823829512 22.076512325003712 44-45 22.24050187860456 26.7472696368503 28.983290016898856 22.028938467646284 46-47 22.51417123819761 26.877213414566974 28.86959900690095 21.73901634033447 48-49 22.35740270242896 27.138674492269782 28.71957952959228 21.784343275708977 50-51 22.607937834154054 26.98237894543789 28.39533675420805 22.014346466200006 52-53 22.68059062193408 27.1320382077467 28.162779048864333 22.024592121454884 54-55 22.669004098155572 27.302594769601384 27.871129634656977 22.157271497586066 56-57 22.875972631536772 27.571222681399842 27.546513696858216 22.00629099020517 58-59 22.910653764369393 27.807332769093286 27.05080665827878 22.231206808258538 60-61 23.142788997825846 27.878505413226286 26.658411357075053 22.320294231872815 62-63 22.828350232845903 27.933791233265957 27.053720984033365 22.184137549854775 64-65 23.074420075672045 28.230202339237216 26.498047455932287 22.197330129158452 66-67 22.772196120830653 28.365143372126095 26.503486006215056 22.359174500828196 68-69 22.679062897950494 28.29569737152397 26.56909649899019 22.456143231535346 70-71 22.59347941095988 28.269754525264535 26.584508719339656 22.552257344435926 72-73 22.496546003290746 28.212671659793788 26.51951642433937 22.771265912576098 74-75 22.45124648684486 28.15123590712247 26.500431255672506 22.897086350360162 76-77 22.457848184721655 28.111717358092687 26.38181686660363 23.04861759058203 78-79 22.439315783817353 28.071994019072616 26.22485192187477 23.263838275235262 80-81 22.38907417975539 28.065387496994653 26.115910361896056 23.4296279613539 82-83 22.31169220452303 28.07500927960749 26.016151630146123 23.59714688572336 84-85 22.236425245650786 27.984678553938082 25.97006077099524 23.808835429415893 86-87 22.167944524829057 27.896638958894698 25.971001190070766 23.964415326205476 88-89 22.1064721694305 27.840912628861776 25.960257580675844 24.092357621031883 90-91 22.054606142801894 27.67815989943429 25.970052504661766 24.297181453102052 92-93 21.990604926312447 27.569668894608046 26.04016737826887 24.39955880081064 94-95 21.931290706195345 27.509678323808384 26.104688976646745 24.454341993349527 96-97 21.87402762488288 27.39296805388748 26.142727392488112 24.590276928741524 98-99 21.811686824825337 27.32467081909775 26.196980209797577 24.666662146279332 100 21.760857538655507 27.350184998783444 26.15303241523644 24.73592504732461 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 137036.0 1 80987.0 2 17259.0 3 8305.5 4 6597.0 5 6009.5 6 5796.5 7 5727.5 8 5689.5 9 5767.0 10 6445.0 11 11162.5 12 114870.0 13 131441.5 14 44658.0 15 42801.5 16 47549.5 17 53131.5 18 58896.0 19 65423.0 20 75371.5 21 91855.0 22 114571.0 23 141150.0 24 172720.5 25 210600.5 26 250736.0 27 299371.5 28 358916.5 29 418939.0 30 478256.5 31 542370.5 32 611361.0 33 680192.0 34 744244.0 35 816561.0 36 905742.5 37 980094.0 38 1047541.5 39 1115924.5 40 1165637.5 41 1218164.5 42 1276885.0 43 1308823.0 44 1335633.5 45 1384849.0 46 1424768.0 47 1448255.5 48 1475838.5 49 1508211.0 50 1535408.5 51 1551843.5 52 1543963.0 53 1499592.5 54 1432378.5 55 1358263.5 56 1269186.5 57 1158020.0 58 1026245.5 59 872610.0 60 707970.0 61 563165.5 62 442351.5 63 332770.5 64 246121.5 65 182812.0 66 131881.5 67 96046.0 68 71074.0 69 52559.0 70 40701.0 71 34317.5 72 29012.5 73 20940.0 74 14285.5 75 10523.5 76 7200.0 77 4050.0 78 2184.0 79 1293.0 80 765.0 81 502.0 82 302.5 83 174.5 84 101.0 85 61.0 86 50.5 87 35.0 88 15.5 89 6.0 90 5.0 91 3.5 92 2.0 93 1.5 94 0.5 95 1.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06951763288202248 2 0.03671050810975881 3 0.03828564388277072 4 9.647706609697943E-4 5 8.860138723191988E-5 6 0.0033668527148129563 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 5.35300047859516E-4 20-21 6.768161524660548E-4 22-23 4.6023498367691717E-4 24-25 8.478660528165667E-4 26-27 0.00805411221434605 28-29 0.010201465279897442 30-31 0.0125715523883513 32-33 0.01259124158551395 34-35 0.015624608523384539 36-37 0.021003451073255675 38-39 0.017538152372629477 40-41 0.02152029249877521 42-43 0.02448720839622186 44-45 0.021770099187776316 46-47 0.022360775102655783 48-49 0.03271360108574109 50-51 0.011024719836260698 52-53 0.0256968634469021 54-55 0.05877717582979752 56-57 0.05588655557135614 58-59 0.04742758424035312 60-61 0.06606587050444561 62-63 0.035242432346318804 64-65 0.023172954485615047 66-67 0.02832906299258372 68-69 0.023650417516809283 70-71 0.012598625034449942 72-73 0.005012131252716801 74-75 0.004448527983935978 76-77 0.004993672630376819 78-79 0.0039341477080617766 80-81 0.004318087052733429 82-83 0.0016206670414505347 84-85 0.0029041565814907078 86-87 0.0026112797736963056 88-89 0.0014151610460653872 90-91 1.8089449893183646E-4 92-93 8.294074304765834E-4 94-95 0.0022937914694485927 96-97 0.002954610149219995 98-99 0.00167727348329315 100 8.367908794125767E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.0631418E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.15539527667444 #Duplication Level Percentage of deduplicated Percentage of total 1 73.22186464895194 30.13474782522771 2 12.653668124631995 10.415334267381711 3 4.69320111329835 5.794516407921665 4 2.4672095275461414 4.061559333461546 5 1.5184924997414415 3.1247079525762245 6 1.0277195669691706 2.5377723007313344 7 0.737221360829623 2.1238445557945673 8 0.5556379825543928 1.8294000642207975 9 0.4280931361690571 1.5856508010841885 >10 2.3264668560595774 17.132030140160612 >50 0.20806431946460077 6.116657642484505 >100 0.16057766628745546 11.072984374738564 >500 0.0013857980543803465 0.3537843163679304 >1k 3.49504723073227E-4 0.26543078106844825 >5k 1.79605242581954E-5 0.04458688114791685 >10k+ 2.993419464565076E-5 3.4069923556320227 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1023453 2.518870987963058 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCAATCTCGTATG 166245 0.4091538227880701 TruSeq Adapter, Index 16 (97% over 38bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 91715 0.22572433972154257 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77282 0.19020256689047868 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.4557901474174492 1.9689197162648865E-5 0.0 0.0 7.3834489359933245E-6 2 0.4558221423628385 2.215034680797997E-5 0.0 2.461149645331108E-6 7.3834489359933245E-6 3 0.4558442927096465 3.1994945389304404E-5 0.0 2.461149645331108E-6 7.3834489359933245E-6 4 0.45587874880468116 5.168414255195327E-5 0.0 4.922299290662216E-6 1.4766897871986649E-5 5 0.4558861322536171 5.660644184261549E-5 0.0 4.922299290662216E-6 1.9689197162648865E-5 6 0.4559008991514891 6.15287411332777E-5 0.0 4.922299290662216E-6 1.9689197162648865E-5 7 0.45591812719900643 6.645104042393992E-5 0.0 4.922299290662216E-6 2.215034680797997E-5 8 0.4559328940968784 6.891219006927103E-5 0.0 4.922299290662216E-6 2.461149645331108E-5 9 0.45594519984510506 7.383448935993324E-5 0.0 4.922299290662216E-6 2.9533795743973298E-5 10-11 0.45599811456247974 8.86013872319199E-5 0.0 7.3834489359933245E-6 2.9533795743973298E-5 12-13 0.456044876405741 9.844598581324432E-5 0.0 7.3834489359933245E-6 4.0608969147963285E-5 14-15 0.4561039439972289 1.1444345850789653E-4 0.0 7.3834489359933245E-6 4.5531268438625496E-5 16-17 0.45613963066708624 1.230574822665554E-4 0.0 7.3834489359933245E-6 5.537586701994993E-5 18-19 0.4562048511326875 1.365938053158765E-4 0.0 9.844598581324433E-6 6.768161524660547E-5 20-21 0.4563192945911954 1.6243587659185314E-4 0.0 1.3536323049321094E-5 7.629563900526435E-5 22-23 0.4565481815082112 1.9196967233582642E-4 0.0 1.4766897871986649E-5 9.598483616791321E-5 24-25 0.4577455308106648 2.1535059396647196E-4 0.0 1.5997472694652202E-5 1.0090713545857543E-4 26-27 0.46276504551231756 2.3627036595178638E-4 0.0 2.215034680797997E-5 1.070600095719032E-4 28-29 0.4781004689523757 2.4488438971044524E-4 0.0 2.215034680797997E-5 1.1075173403989986E-4 30-31 0.5135422544199664 2.6949588616375633E-4 0.0 2.215034680797997E-5 1.1813518297589318E-4 32-33 0.5809088425119695 2.867239336810741E-4 0.0 2.3380921630645525E-5 1.3290208084787983E-4 34-35 0.6942817009241469 3.0641313084372296E-4 0.0 2.5842071275976634E-5 1.390549549612076E-4 36-37 0.8627806196672733 3.3594692658769627E-4 0.0 2.9533795743973295E-5 1.5751357730119092E-4 38-39 1.0927307533298494 3.4702209999168626E-4 0.0 3.1994945389304404E-5 1.6735817588251536E-4 40-41 1.383290388733172 3.580972733956762E-4 0.0 3.568666985730107E-5 1.7104990035051202E-4 42-43 1.7280420781770403 3.790170453809906E-4 0.0 3.937839432529773E-5 1.8089449893183643E-4 44-45 2.127923519676325 3.9501451807564283E-4 0.0 4.183954397062884E-5 1.895085226904953E-4 46-47 2.590140959392557 4.1839543970628837E-4 0.0 4.676184326129105E-5 2.0550599538514752E-4 48-49 3.1112327903495762 4.3193176275560947E-4 0.0 5.0453567729287714E-5 2.2027289325713418E-4 50-51 3.6864760663779936 4.417763613369339E-4 0.0 5.537586701994993E-5 2.3503979112912083E-4 52-53 4.305517961494723 4.5900440885425167E-4 0.0 5.660644184261549E-5 2.485761141784419E-4 54-55 4.96788101266857 4.860770549528938E-4 0.0 6.275931595594325E-5 2.62112437227763E-4 56-57 5.672504464402399 5.069968269382082E-4 0.0 6.891219006927103E-5 2.670347365184252E-4 58-59 6.420865006483407 5.279165989235227E-4 0.0 7.50650641825988E-5 2.7195703580908744E-4 60-61 7.201085130723225 5.586809694901615E-4 0.0 8.86013872319199E-5 2.818016343904119E-4 62-63 8.0078032226195 5.820618911208071E-4 0.0 9.106253687725099E-5 2.8795450850373965E-4 64-65 8.837390317020194 6.103651120421148E-4 0.0 9.106253687725099E-5 2.9902968190772964E-4 66-67 9.693518449196137 6.165179861554426E-4 0.0 9.35236865225821E-5 3.076437056663885E-4 68-69 10.573811379164763 6.362071833180915E-4 0.0 9.598483616791321E-5 3.248717531837063E-4 70-71 11.472388189848555 6.448212070767503E-4 0.0 1.0090713545857543E-4 3.297940524743685E-4 72-73 12.386011238889079 6.645104042393992E-4 0.0 1.0090713545857543E-4 3.347163517650307E-4 74-75 13.31122138045982 6.780467272887203E-4 0.0 1.0213771028124098E-4 3.371775014103618E-4 76-77 14.25067296445327 6.940441999833725E-4 0.0 1.0336828510390654E-4 3.4456095034635515E-4 78-79 15.205664247307343 7.137333971460213E-4 0.0 1.0336828510390654E-4 3.519443992823485E-4 80-81 16.170028818585656 7.297308698406735E-4 0.0 1.1075173403989986E-4 3.5686669857301064E-4 82-83 17.141413819227278 7.469589173579913E-4 0.0 1.1690460815322764E-4 3.6178899786367286E-4 84-85 18.119494377479022 7.666481145206401E-4 0.0 1.230574822665554E-4 3.654807223316695E-4 86-87 19.103084219211844 7.764927131019646E-4 0.0 1.255186319118865E-4 3.7040302162233174E-4 88 19.843270052745883 7.826455872152923E-4 0.0 1.2797978155721761E-4 3.765558957356595E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 141465 0.0 80.39972 8 GTATCAA 144060 0.0 78.71989 9 AGTGGTA 145045 0.0 78.544426 5 GTGGTAT 145330 0.0 78.38678 6 TGGTATC 145660 0.0 78.04228 7 AAGCAGT 156515 0.0 73.52214 1 CAGTGGT 157120 0.0 72.738846 4 GCAGTGG 158970 0.0 72.11296 3 AGCAGTG 172035 0.0 66.91069 2 CAACGCA 135565 0.0 43.897522 12-13 ATCAACG 138345 0.0 43.091564 10-11 ACGCAGA 141730 0.0 41.980137 14-15 TCAACGC 135175 0.0 41.794376 12-13 AGAGTAC 144190 0.0 40.978493 18-19 AACGCAG 138730 0.0 40.60688 14-15 GAGTACT 136630 0.0 39.685608 20-21 AGTACTT 148590 0.0 38.74771 20-21 CGCAGAG 145930 0.0 38.502186 16-17 TATCAAC 147330 0.0 38.483154 10-11 GTACTTT 146360 0.0 36.95722 22-23 >>END_MODULE