FastQCFastQC Report
Thu 26 May 2016
SRR1660660_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660660_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37942589
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3201990.8439039307517998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1252360.33006709162624615No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA556250.069.052248
GTATCAA586650.065.350879
TGGTATC591850.065.187217
GTGGTAT593750.065.157686
AGTGGTA596500.065.092265
AAGCAGT742900.052.8783261
GCAGTGG737900.052.8607183
CAGTGGT738400.052.793144
AGCAGTG863900.045.5644952
CAACGCA500000.040.8611612-13
ATCAACG524150.039.05084610-11
TCAACGC494800.038.653912-13
ACGCAGA550650.037.42794814-15
AACGCAG524600.036.6122714-15
AGAGTAC576700.035.4182118-19
GAGTACT524350.033.97828720-21
CGCAGAG569150.033.75055716-17
TATCAAC604600.031.88690810-11
AGTACTT643150.029.68433220-21
GTACTTT627450.028.39710222-23
Produced by FastQC (version 0.11.5)