FastQCFastQC Report
Thu 26 May 2016
SRR1660660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37942589
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7884012.0778787657320907No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT993320.2617955248125003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA474860.12515223987482774No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1111000.078.411658
GTGGTAT1147900.076.0664756
TGGTATC1145350.076.051827
AGTGGTA1150950.075.9711465
GTATCAA1146450.075.8231059
AAGCAGT1260900.070.105611
GCAGTGG1282550.068.630313
CAGTGGT1291600.067.9640354
AGCAGTG1409850.062.778212
CAACGCA1056600.043.46794512-13
ATCAACG1080250.042.58574710-11
ACGCAGA1102450.041.6991914-15
TCAACGC1053100.041.11612712-13
AGAGTAC1141200.040.01303518-19
AACGCAG1083150.039.9001814-15
GAGTACT1076550.038.53604520-21
CGCAGAG1124350.038.3699116-17
TATCAAC1160000.037.5051610-11
AGTACTT1193250.037.10445820-21
GTACTTT1178250.035.12380222-23
Produced by FastQC (version 0.11.5)