##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660660_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37942589 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72491845509013 34.0 31.0 34.0 31.0 34.0 2 32.904382592342344 34.0 31.0 34.0 31.0 34.0 3 32.90502414055087 34.0 31.0 34.0 31.0 34.0 4 36.31839764545324 37.0 37.0 37.0 35.0 37.0 5 36.24064193405463 37.0 37.0 37.0 35.0 37.0 6 36.23142195172817 37.0 37.0 37.0 35.0 37.0 7 36.208945836563764 37.0 37.0 37.0 35.0 37.0 8 36.21401206965608 37.0 37.0 37.0 35.0 37.0 9 37.98427534821095 39.0 38.0 39.0 35.0 39.0 10-11 37.95218690796244 39.0 38.0 39.0 35.0 39.0 12-13 37.96957598228207 39.0 38.0 39.0 35.0 39.0 14-15 39.485244259425734 41.0 39.0 41.0 36.0 41.0 16-17 39.45389090343835 41.0 39.0 41.0 36.0 41.0 18-19 39.44625536755017 41.0 39.0 41.0 36.0 41.0 20-21 39.43403079320707 41.0 39.0 41.0 36.0 41.0 22-23 39.35692364060871 41.0 39.0 41.0 36.0 41.0 24-25 39.34515467566011 41.0 39.0 41.0 36.0 41.0 26-27 39.286023115080525 41.0 39.0 41.0 36.0 41.0 28-29 39.204262537277046 41.0 39.0 41.0 36.0 41.0 30-31 39.151216763305214 41.0 39.0 41.0 36.0 41.0 32-33 39.074106632523154 41.0 39.0 41.0 35.0 41.0 34-35 39.008012513326385 40.0 39.0 41.0 35.0 41.0 36-37 38.91221261943933 40.0 38.5 41.0 35.0 41.0 38-39 38.70525703978714 40.0 38.0 41.0 35.0 41.0 40-41 38.62455145061398 40.0 38.0 41.0 35.0 41.0 42-43 38.54607949921393 40.0 38.0 41.0 35.0 41.0 44-45 38.45238942445388 40.0 38.0 41.0 34.0 41.0 46-47 38.37891122822431 40.0 38.0 41.0 34.0 41.0 48-49 38.320827250349204 40.0 38.0 41.0 34.0 41.0 50-51 38.24387661052861 40.0 37.5 41.0 34.0 41.0 52-53 38.08543483946232 40.0 37.0 41.0 33.5 41.0 54-55 37.75373570316986 40.0 37.0 41.0 33.0 41.0 56-57 37.4968143449568 40.0 36.5 41.0 33.0 41.0 58-59 37.27741554483802 39.5 36.0 41.0 32.0 41.0 60-61 37.026942995376515 39.0 35.5 41.0 32.0 41.0 62-63 36.81702708795122 39.0 35.0 41.0 31.5 41.0 64-65 36.53975230841522 38.5 35.0 40.5 31.0 41.0 66-67 36.26232499579825 37.5 35.0 40.0 31.0 41.0 68-69 35.95245037179724 37.0 35.0 40.0 31.0 41.0 70-71 35.63088244979804 36.5 35.0 39.0 31.0 41.0 72-73 35.285510327194594 36.0 35.0 39.0 30.5 41.0 74-75 34.9252318417175 35.5 34.5 38.5 30.0 40.0 76-77 34.00253409170365 35.0 33.5 37.0 29.5 39.0 78-79 34.35188036325091 35.0 34.0 37.0 30.0 39.0 80-81 34.14026942125642 35.0 34.0 36.5 30.0 39.0 82-83 33.90349560753485 35.0 34.0 36.0 30.0 37.5 84-85 33.683752484576104 35.0 34.0 36.0 30.0 37.0 86-87 33.49349070512821 35.0 34.0 35.0 30.0 37.0 88-89 33.33513387818633 35.0 34.0 35.0 29.0 36.0 90-91 33.19809477155078 35.0 34.0 35.0 29.0 36.0 92-93 33.072748422623455 35.0 34.0 35.0 29.0 36.0 94-95 32.98621307839589 35.0 34.0 35.0 29.0 35.5 96-97 32.90092680549553 35.0 34.0 35.0 29.0 35.0 98-99 32.82641844761832 35.0 34.0 35.0 29.0 35.0 100 32.80260037078651 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04553353528544335 1101 2 0.038836769948133565 1101 3 0.014880889523929852 1101 4 0.011816487814911625 1101 5 0.01089903558918337 1101 6 0.03623958882661782 1101 7 0.03528716261388354 1101 8 0.0203211353559567 1101 9 0.03660723693943879 1101 10-11 0.06312600863405038 1101 12-13 0.03439198694970713 1101 14-15 0.06425858474548107 1101 16-17 0.06134791436324605 1101 18-19 0.07885257725326511 1101 20-21 0.07101678758610319 1101 22-23 0.08523771988933504 1101 24-25 0.09389607323901572 1101 26-27 0.07817102214321636 1101 28-29 0.12886139776943395 1101 30-31 0.09012159517637741 1101 32-33 0.1053394614177634 1101 34-35 0.11489452878603856 1101 36-37 0.11746320122032472 1101 38-39 0.1650618291957855 1101 40-41 0.13929976695014545 1101 42-43 0.14553619304683707 1101 44-45 0.1692572115958555 1101 46-47 0.12384628018374144 1101 48-49 0.12895351714335845 1101 50-51 0.10966924894609065 1101 52-53 0.09141958166657105 1101 54-55 0.12825435929939744 1101 56-57 0.08332302545206005 1101 58-59 0.09005583058331723 1101 60-61 0.08687519510773711 1101 62-63 0.07068571428088433 1101 64-65 0.09934399269307193 1101 66-67 0.08139199525266605 1101 68-69 0.08458060239357224 1101 70-71 0.05169895734798757 1101 72-73 0.05872618539521568 1101 74-75 0.06896587872127213 1101 76-77 0.3651782146015776 1101 78-79 0.08201069937137362 1101 80-81 0.06764726503651985 1101 82-83 0.05337694125137915 1101 84-85 0.04550720735895908 1101 86-87 0.061965209993871895 1101 88-89 0.049643068731079154 1101 90-91 0.03874609761994208 1101 92-93 0.06209514917534875 1101 94-95 0.06753284187349351 1101 96-97 0.060035320158668526 1101 98-99 0.06528068651235941 1101 100 0.059155267871588535 1102 1 0.015485070491564557 1102 2 0.013743881636841593 1102 3 -0.013971145624047665 1102 4 0.012099751547360427 1102 5 0.008566911805644395 1102 6 0.03099622485756015 1102 7 0.031777041479472246 1102 8 0.019659200105962782 1102 9 0.03963857372974644 1102 10-11 0.026856886687873782 1102 12-13 0.025441511963123276 1102 14-15 3.585903847849181E-4 1102 16-17 0.03152474980497999 1102 18-19 0.046087121765459926 1102 20-21 0.040183194295018154 1102 22-23 0.049819537996953045 1102 24-25 0.046419567838242415 1102 26-27 0.0463619801631836 1102 28-29 0.06588199156725949 1102 30-31 0.08953158888004964 1102 32-33 0.06042424188019169 1102 34-35 0.0814208781312189 1102 36-37 0.08432817653083191 1102 38-39 0.11456407375352029 1102 40-41 0.12574948786529916 1102 42-43 0.1265256817312661 1102 44-45 0.1382638281826729 1102 46-47 0.12540384073341215 1102 48-49 0.09148868648505726 1102 50-51 0.02890310596571055 1102 52-53 0.058106218620309846 1102 54-55 0.08739738347002657 1102 56-57 0.05495418878653879 1102 58-59 -0.014977954987138276 1102 60-61 0.03186753516533258 1102 62-63 0.001667589736044306 1102 64-65 -0.004104411029700827 1102 66-67 6.71873986043181E-4 1102 68-69 -0.002862114569886387 1102 70-71 -0.01151382270484902 1102 72-73 0.018545025952931837 1102 74-75 0.03646182707348089 1102 76-77 0.32542020112337156 1102 78-79 0.09069096753715655 1102 80-81 0.06206498419903994 1102 82-83 0.05650218915141636 1102 84-85 0.042782353849325716 1102 86-87 0.034057001524580244 1102 88-89 0.01193000919203513 1102 90-91 0.0342449560487168 1102 92-93 0.02060688832123958 1102 94-95 0.029795023950974553 1102 96-97 0.05821988876606099 1102 98-99 0.04151742141527848 1102 100 0.015683678159831516 1103 1 -0.022205190286321397 1103 2 -0.03822674602566423 1103 3 -0.058561370731524676 1103 4 -0.04588153206126577 1103 5 -0.024901867513406728 1103 6 -0.03516701298334368 1103 7 -0.04800631438129699 1103 8 -0.04307319464908943 1103 9 -0.05122748538707356 1103 10-11 -0.0451772570703497 1103 12-13 -0.042227992040430706 1103 14-15 -0.048808812941672386 1103 16-17 -0.047255419648962516 1103 18-19 -0.03909425265749178 1103 20-21 -0.05109819240434632 1103 22-23 -0.0553137062156992 1103 24-25 -0.03160538766320542 1103 26-27 -0.05441382109213322 1103 28-29 -0.009604922617370448 1103 30-31 -0.018596411482839414 1103 32-33 -0.073998724479452 1103 34-35 -0.009520029894055426 1103 36-37 0.004620329876743767 1103 38-39 0.031015415373140343 1103 40-41 0.0697235059491561 1103 42-43 0.04684554745616509 1103 44-45 0.04102379648786325 1103 46-47 0.028782915934883135 1103 48-49 0.034884704888582974 1103 50-51 -0.02867892638302294 1103 52-53 -0.05454015866713746 1103 54-55 -0.04380985650346503 1103 56-57 -0.026037884524171773 1103 58-59 -0.05618783093560609 1103 60-61 -0.020597146666297306 1103 62-63 -0.06320580463222569 1103 64-65 -0.03788471159640494 1103 66-67 -0.04963055529252358 1103 68-69 -0.04926555738073546 1103 70-71 -0.07827224963727986 1103 72-73 -0.03758451852667832 1103 74-75 -0.04268701946540432 1103 76-77 0.269911333951832 1103 78-79 0.03425906237747256 1103 80-81 -0.010399215670290118 1103 82-83 -0.02905602819560471 1103 84-85 -0.03043879054816756 1103 86-87 -0.0209896775406051 1103 88-89 -0.0035268202542582117 1103 90-91 -0.01819923336962148 1103 92-93 0.01448448080416398 1103 94-95 -0.020745875229216892 1103 96-97 -0.029940225420020283 1103 98-99 -0.010110855972691013 1103 100 -0.04622772263572017 1104 1 -0.042491628219345046 1104 2 -0.05399469313044847 1104 3 -0.07395551190383287 1104 4 -0.02386946493739117 1104 5 -0.04172901641359772 1104 6 -0.01782954136650261 1104 7 -0.028621846597964407 1104 8 -0.035171580993271334 1104 9 -0.045981957101155047 1104 10-11 -0.05406323539455116 1104 12-13 -0.05429032570788905 1104 14-15 -0.08399818444160445 1104 16-17 -0.06639042518430216 1104 18-19 -0.05925538586558332 1104 20-21 -0.05429789267475371 1104 22-23 -0.03723050767775504 1104 24-25 -0.06665325363390906 1104 26-27 -0.05310142644063376 1104 28-29 -0.026096069207532935 1104 30-31 -0.04251354026332166 1104 32-33 -0.04962459346820225 1104 34-35 -0.04837580999903679 1104 36-37 -0.02908966613357933 1104 38-39 0.003456588892007062 1104 40-41 0.0344059568394357 1104 42-43 -0.03499236075015233 1104 44-45 -0.027401874730998088 1104 46-47 -0.017191645744752293 1104 48-49 -0.031499996603983504 1104 50-51 -0.09340420025505125 1104 52-53 -0.101124463257662 1104 54-55 -0.05647277665404005 1104 56-57 -0.06203149333521196 1104 58-59 -0.09132569452383876 1104 60-61 -0.08341722247443784 1104 62-63 -0.11286219941382569 1104 64-65 -0.10517125730593335 1104 66-67 -0.10336864742164664 1104 68-69 -0.11770538543726161 1104 70-71 -0.1301874858484453 1104 72-73 -0.0981564343330632 1104 74-75 -0.09734752402924585 1104 76-77 0.17383653630055562 1104 78-79 -0.010336837852428005 1104 80-81 -0.006839758940877516 1104 82-83 -0.023026171871585177 1104 84-85 -0.053246450380228794 1104 86-87 -0.06313522647482728 1104 88-89 -0.04838930339285241 1104 90-91 -0.06897447992063377 1104 92-93 -0.05438509207149167 1104 94-95 -0.08613136984654801 1104 96-97 -0.0659554749004414 1104 98-99 -0.044778506059167 1104 100 -0.0870175198771932 1105 1 -0.10614186247185842 1105 2 -0.09234720181132161 1105 3 -0.10649686326156171 1105 4 -0.07433723323354258 1105 5 -0.09118741634050309 1105 6 -0.07209161158917254 1105 7 -0.07819621273808508 1105 8 -0.053575996135947435 1105 9 -0.09199155960548921 1105 10-11 -0.1186804356099529 1105 12-13 -0.11310345338826977 1105 14-15 -0.12496968023722843 1105 16-17 -0.1129919762146514 1105 18-19 -0.10556852281388984 1105 20-21 -0.09944312280067891 1105 22-23 -0.12453802749345755 1105 24-25 -0.10120251895706645 1105 26-27 -0.12145437952089111 1105 28-29 -0.08513322829013958 1105 30-31 -0.09293653671620206 1105 32-33 -0.09599730671246931 1105 34-35 -0.11984052956353253 1105 36-37 -0.1034364720499994 1105 38-39 -0.09217744513376402 1105 40-41 -0.11139887574076823 1105 42-43 -0.0996984987085554 1105 44-45 -0.08268508970435562 1105 46-47 -0.10461361479773501 1105 48-49 -0.11314489642292358 1105 50-51 -0.17272562147370252 1105 52-53 -0.1593075114843856 1105 54-55 -0.1345910978669096 1105 56-57 -0.17057107287443074 1105 58-59 -0.18171525618696904 1105 60-61 -0.14325494403225036 1105 62-63 -0.16179105029958407 1105 64-65 -0.15079006737601475 1105 66-67 -0.1940385974725558 1105 68-69 -0.19820689539028535 1105 70-71 -0.18459163760513064 1105 72-73 -0.1851335415443387 1105 74-75 -0.17218906969312542 1105 76-77 0.1754424107100121 1105 78-79 -0.05314028075564181 1105 80-81 -0.09791947832705006 1105 82-83 -0.12430800497559602 1105 84-85 -0.12605695907466696 1105 86-87 -0.1446696225112305 1105 88-89 -0.11338873803057226 1105 90-91 -0.09918185098924681 1105 92-93 -0.08905841610649645 1105 94-95 -0.08332705150542807 1105 96-97 -0.09026478231928792 1105 98-99 -0.09346961465821124 1105 100 -0.09260696019203607 1106 1 -0.1405507693253938 1106 2 -0.12919700108881216 1106 3 -0.16103717079108293 1106 4 -0.09080053914608044 1106 5 -0.1138183325108102 1106 6 -0.11270482512093594 1106 7 -0.08115423566631819 1106 8 -0.09412898266138114 1106 9 -0.11238097014641113 1106 10-11 -0.13693531385629854 1106 12-13 -0.12023151898821283 1106 14-15 -0.16016786243594794 1106 16-17 -0.16896046102562678 1106 18-19 -0.15316549832448345 1106 20-21 -0.1394291769349607 1106 22-23 -0.1345530580359693 1106 24-25 -0.11599135936533855 1106 26-27 -0.14756933671216643 1106 28-29 -0.16363620457861572 1106 30-31 -0.13729439975160318 1106 32-33 -0.13501773288071917 1106 34-35 -0.09948178605550595 1106 36-37 -0.1175899476326876 1106 38-39 -0.08287404113917773 1106 40-41 -0.0933759074110796 1106 42-43 -0.11071043211106968 1106 44-45 -0.10214670269265724 1106 46-47 -0.08043311351615756 1106 48-49 -0.1333475946997993 1106 50-51 -0.17929955180684942 1106 52-53 -0.1820256884585234 1106 54-55 -0.16171824639069854 1106 56-57 -0.1896974451340725 1106 58-59 -0.23295277349722454 1106 60-61 -0.254867709621017 1106 62-63 -0.2863675396898202 1106 64-65 -0.243193499972854 1106 66-67 -0.2582264800358871 1106 68-69 -0.2504165776111691 1106 70-71 -0.2262910194885137 1106 72-73 -0.22480598672454022 1106 74-75 -0.23331408977622914 1106 76-77 0.14768598340143768 1106 78-79 -0.09732524283669619 1106 80-81 -0.11254097490367343 1106 82-83 -0.13208748033328277 1106 84-85 -0.13994243014450802 1106 86-87 -0.16660125788977354 1106 88-89 -0.14589430180304674 1106 90-91 -0.17061341813874975 1106 92-93 -0.1596751712440465 1106 94-95 -0.12014626408613793 1106 96-97 -0.13312529775398474 1106 98-99 -0.17662956703281196 1106 100 -0.21883994301647647 1107 1 -0.14314005288364484 1107 2 -0.13576992666640564 1107 3 -0.1400008084788098 1107 4 -0.09572831801980186 1107 5 -0.1509161265969894 1107 6 -0.1261628618484565 1107 7 -0.10610205332874756 1107 8 -0.11341603761974994 1107 9 -0.13654290224547339 1107 10-11 -0.13149471227703202 1107 12-13 -0.1316212142550981 1107 14-15 -0.19007211505055466 1107 16-17 -0.15135671098405368 1107 18-19 -0.16126086001964524 1107 20-21 -0.16155728008502734 1107 22-23 -0.17843838704902737 1107 24-25 -0.16939983616777 1107 26-27 -0.1611155173765937 1107 28-29 -0.16402030379590826 1107 30-31 -0.19221015770443728 1107 32-33 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0.5862465214837442 2315 70-71 0.5743196058640336 2315 72-73 0.5531143283871387 2315 74-75 0.5476728802309125 2315 76-77 -0.4842035186570115 2315 78-79 0.23002021678573215 2315 80-81 0.3809658009447716 2315 82-83 0.3960472965515507 2315 84-85 0.41464623888831653 2315 86-87 0.38527564115680946 2315 88-89 0.37490153735590326 2315 90-91 0.38468967901224715 2315 92-93 0.38102431753428334 2315 94-95 0.38112909616790347 2315 96-97 0.40154664242323435 2315 98-99 0.41679298645792784 2315 100 0.3855288486236148 2316 1 0.3349942098736278 2316 2 0.22243400116703071 2316 3 0.30680432620719245 2316 4 0.19843503928188255 2316 5 0.2703477345238312 2316 6 0.27200448419700507 2316 7 0.28183275138002983 2316 8 0.28245685331577164 2316 9 0.37110906142379463 2316 10-11 0.3724212812312686 2316 12-13 0.36610128119063745 2316 14-15 0.45908896230577767 2316 16-17 0.44191812512307393 2316 18-19 0.4352866148700727 2316 20-21 0.4099659750507314 2316 22-23 0.24369691638258928 2316 24-25 0.05860905771527314 2316 26-27 0.30512950835058206 2316 28-29 0.3669921412270085 2316 30-31 0.4038890324031925 2316 32-33 0.4115101430337731 2316 34-35 0.38679068573921427 2316 36-37 0.37363855138210056 2316 38-39 0.1727044365952679 2316 40-41 0.20359799073521856 2316 42-43 0.3069113027785093 2316 44-45 0.34746008408536255 2316 46-47 0.3440609061540272 2316 48-49 0.19599294843112602 2316 50-51 0.497750399525863 2316 52-53 0.571836074304855 2316 54-55 0.6263608888442107 2316 56-57 0.24289543042839057 2316 58-59 0.452012403520591 2316 60-61 0.2768512959049758 2316 62-63 0.4641228748213422 2316 64-65 0.5256861162311353 2316 66-67 0.5535973704161705 2316 68-69 0.5177863218623315 2316 70-71 0.5151863443545892 2316 72-73 0.1969612520928905 2316 74-75 0.4236117486839319 2316 76-77 -0.16897761645375198 2316 78-79 0.3128080744815307 2316 80-81 0.2535643883453176 2316 82-83 0.15155567694250038 2316 84-85 0.0021629904783324605 2316 86-87 0.2085249294476199 2316 88-89 0.24445002019571405 2316 90-91 0.3215432568098535 2316 92-93 0.3413073237422708 2316 94-95 0.2924823944850843 2316 96-97 0.334300086727211 2316 98-99 0.352863678249868 2316 100 0.3297581438625059 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 43.0 8 124.0 9 139.0 10 117.0 11 112.0 12 166.0 13 542.0 14 1735.0 15 5016.0 16 10395.0 17 16430.0 18 22884.0 19 30696.0 20 41654.0 21 58319.0 22 85152.0 23 133119.0 24 212217.0 25 296681.0 26 344017.0 27 346996.0 28 359199.0 29 409336.0 30 491899.0 31 601711.0 32 747812.0 33 956601.0 34 1308438.0 35 2059979.0 36 3727198.0 37 8642265.0 38 1.3087257E7 39 3936477.0 40 7862.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.5603477991175 31.328444095028946 27.07966207500589 24.031546030847668 2 21.655252747503067 28.91198857507803 29.36873077232962 20.064027905089286 3 21.640007715931382 27.21408260834761 27.502272540069633 23.643637135651367 4 19.853562670710147 28.037059911964075 27.584730241795434 24.524647175530344 5 21.85411777257365 29.4887755573215 27.41516964910531 21.241937020999536 6 23.89929907750273 26.959958137264294 27.823179386640497 21.317563398592483 7 21.576284633607894 27.517291980259966 29.53583900139234 21.370584384739796 8 23.093653414109404 26.738309818552448 28.124525187250665 22.043511580087486 9 23.241856268690572 26.24962940720782 28.617596442878472 21.890917881223128 10-11 21.31925947383295 28.293642534514447 28.715238699183125 21.671859292469474 12-13 21.414119895719296 27.171722520042056 28.450031440922498 22.964126143316154 14-15 21.401593075264316 29.626942431366505 27.215893201172964 21.75557129219622 16-17 22.750255655985942 27.031372318847296 27.238705297627426 22.979666727539335 18-19 21.550788112012103 28.251736990664867 27.19261892049973 23.0048559768233 20-21 22.81352002200075 28.224127238087878 27.18114491038569 21.781207829525687 22-23 22.768482367851544 27.05545542208208 28.411771391711056 21.764290818355317 24-25 21.668693079293966 28.135398696376935 27.067544428565345 23.12836379576375 26-27 21.71960116431211 26.9474780804541 29.536617176140524 21.79630357909327 28-29 21.794953539572894 26.985741789154172 29.46882822419185 21.750476447081077 30-31 21.800705264426316 26.957253362227384 29.401259863361982 21.840781509984314 32-33 21.82986297367982 27.00720947615124 29.3495020782593 21.813425471909646 34-35 21.84317262408807 27.081624366127176 29.289196916212706 21.786006093572045 36-37 21.85918918286249 27.068056990486124 29.25760769782165 21.815146128829735 38-39 21.926583155145245 27.059853892379003 29.25333979020637 21.760223162269384 40-41 21.931878838367947 27.151182073559717 29.22564967560853 21.69128941246381 42-43 21.970191017424803 27.16732363391226 29.128205176873422 21.734280171789514 44-45 22.012828329516122 27.225836012223255 29.08114349231975 21.680192165940877 46-47 22.063316712682635 27.32935766693747 28.98517722944317 21.622148390936726 48-49 22.137694498792975 27.360148630245 28.835279134716934 21.66687773624509 50-51 22.16735586634024 27.434943373673498 28.74903900262238 21.648661757363882 52-53 22.248877428141864 27.570617651586183 28.53250255102707 21.648002369244885 54-55 22.43377786115558 27.71564884914924 28.09050877046719 21.760064519228 56-57 22.6124944590343 27.968267308051853 27.606773519075556 21.812464713838292 58-59 22.648221548144036 28.17759893884234 27.360854170109285 21.813325342904342 60-61 22.700944834306053 28.233118509658034 27.20728277114827 21.85865388488764 62-63 22.64807754704844 28.298451501945326 27.146721355322235 21.906749595684 64-65 22.68745315028024 28.394081333729858 27.0313994333147 21.8870660826752 66-67 22.66734615198861 28.364139087422142 26.970253474633658 21.998261285955586 68-69 22.653226733457206 28.335377502725734 26.98156765211699 22.029828111700077 70-71 22.631254831725542 28.36868319882853 26.918442647462303 22.08161932198362 72-73 22.617469613369607 28.388011356369514 26.794478427556495 22.200040602704384 74-75 22.644367770976512 28.44614564799614 26.678082554637093 22.23140402639025 76-77 22.689720742912275 28.558427092272165 26.482473799007284 22.269378365808276 78-79 22.75468267217409 28.64196323011687 26.223160532292965 22.38019356541607 80-81 22.783762728280657 28.72727261705348 26.059045611615073 22.42991904305079 82-83 22.784962374546293 28.830255083593592 25.878542118088365 22.50624042377175 84-85 22.800684539085257 28.838953985987143 25.735870819037398 22.6244906558902 86-87 22.82191344136761 28.820008008379737 25.677721160657622 22.680357389595024 88-89 22.84938504045437 28.837472175958638 25.590800307669536 22.722342475917458 90-91 22.874790190851456 28.792365219509612 25.507336841649032 22.8255077479899 92-93 22.919013050596973 28.743267169007297 25.484269527618142 22.85345025277758 94-95 22.93728978681116 28.71632722848762 25.473095354040698 22.873287630660528 96-97 22.94740160798322 28.641492295805755 25.4418536743466 22.969252421864432 98-99 22.92587995458479 28.590277683792742 25.46031578189562 23.02352657972685 100 22.898363124374658 28.617450801446186 25.42121214141828 23.062973932760872 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 123611.0 1 73595.5 2 16280.0 3 7605.0 4 5775.0 5 5100.0 6 4877.5 7 4731.0 8 4639.5 9 4723.5 10 5227.0 11 9169.5 12 90182.0 13 102451.0 14 34265.5 15 33184.5 16 37820.5 17 43747.0 18 49904.5 19 57230.0 20 68633.5 21 87632.5 22 114514.0 23 145741.5 24 180178.0 25 220267.0 26 266064.0 27 321615.0 28 390473.0 29 459038.5 30 518777.0 31 581122.5 32 647557.0 33 706991.0 34 763576.5 35 832480.5 36 909596.0 37 970485.0 38 1031985.5 39 1093350.5 40 1136503.0 41 1189913.0 42 1253472.0 43 1280842.0 44 1290707.5 45 1322843.5 46 1351735.5 47 1368003.5 48 1382640.5 49 1399199.0 50 1411162.5 51 1408638.5 52 1384360.0 53 1330252.5 54 1256842.5 55 1174568.0 56 1083469.5 57 977534.5 58 856103.0 59 722203.5 60 581706.0 61 459538.0 62 357652.0 63 266091.0 64 193415.5 65 140044.5 66 99790.0 67 73093.0 68 53981.5 69 40503.0 70 31590.0 71 25331.5 72 21018.5 73 16132.5 74 12221.5 75 10017.0 76 7265.0 77 4096.0 78 2193.0 79 1363.5 80 827.5 81 531.5 82 329.5 83 181.0 84 110.5 85 75.5 86 42.0 87 21.0 88 14.5 89 11.0 90 6.5 91 2.5 92 2.0 93 2.5 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06667705253323647 2 0.03393811634730566 3 0.04181317200046628 4 9.250818387748922E-4 5 9.75157493865271E-5 6 0.003813656469251479 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.519986762105243E-4 20-21 6.312168102181957E-4 22-23 4.6517648018167657E-4 24-25 8.776417444787439E-4 26-27 0.0081847340464827 28-29 0.010431549623564169 30-31 0.012923472354509071 32-33 0.013047343711837904 34-35 0.015959638389462565 36-37 0.021392846966768662 38-39 0.017883597769250803 40-41 0.02175260101518112 42-43 0.02422343925977218 44-45 0.022400948970561812 46-47 0.022810778674064648 48-49 0.03362580239318935 50-51 0.010911221688114113 52-53 0.025902291485696984 54-55 0.05663951924840975 56-57 0.05402636072093025 58-59 0.04537381463347164 60-61 0.06195280980957836 62-63 0.03289179871199617 64-65 0.021428427037490774 66-67 0.026242278828152714 68-69 0.02180531223106573 70-71 0.011960174884217838 72-73 0.004655718143008112 74-75 0.004235346196328353 76-77 0.0048784230301205855 78-79 0.004125970423367789 80-81 0.004435648816689868 82-83 0.0017249745398238375 84-85 0.0030229882309823403 86-87 0.0028503589989602447 88-89 0.0015312608214478986 90-91 1.858070359932476E-4 92-93 8.960906700383573E-4 94-95 0.0023680513736160703 96-97 0.003190346341415974 98-99 0.0017750501949142165 100 9.646152506883492E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.7942589E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.85142397128637 #Duplication Level Percentage of deduplicated Percentage of total 1 75.84476734339506 34.01745816122187 2 11.371986064240836 10.20099536725652 3 4.2517907896730245 5.720966140345059 4 2.31754946981536 4.157815753804747 5 1.4078359380994592 3.157172327085626 6 0.9294851310418706 2.501323901241937 7 0.6589243396271616 2.0687586445132404 8 0.49763965307502545 1.7855877651993466 9 0.3865676628284717 1.5604299125198158 >10 2.025252293419792 16.40844644649899 >50 0.17847247458345233 5.597285810314563 >100 0.1284330814080157 9.789314575759976 >500 0.0010499610064764711 0.3050665583337784 >1k 2.105425385783674E-4 0.1769280980926765 >5k 1.1751749711305906E-5 0.03118221298726669 >10k+ 2.350349767649888E-5 2.5212683248245873 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 788401 2.0778787657320907 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99332 0.2617955248125003 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47486 0.12515223987482774 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.008449607906302862 0.0 0.0 0.0 1.3177803971152311E-5 2 0.008468056831862475 7.906682382691387E-6 0.0 0.0 1.3177803971152311E-5 3 0.008489141318216318 1.0542243176921848E-5 0.0 2.635560794230462E-6 1.8448925559613235E-5 4 0.008502319122187471 1.0542243176921848E-5 0.0 5.271121588460924E-6 2.1084486353843697E-5 5 0.008512861365364393 1.3177803971152311E-5 0.0 7.906682382691387E-6 2.1084486353843697E-5 6 0.008518132486952854 1.8448925559613235E-5 0.0 7.906682382691387E-6 2.1084486353843697E-5 7 0.008520768047747084 2.8991168736535084E-5 0.0 7.906682382691387E-6 2.372004714807416E-5 8 0.008539216973306698 3.162672953076555E-5 0.0 1.0542243176921848E-5 2.8991168736535084E-5 9 0.00855503033807208 3.953341191345693E-5 0.0 1.0542243176921848E-5 2.8991168736535084E-5 10-11 0.008577432604823038 5.1393435487494015E-5 0.0 1.0542243176921848E-5 3.294450992788078E-5 12-13 0.008603788212765345 5.666455707595494E-5 0.0 1.3177803971152311E-5 4.085119231057216E-5 14-15 0.008636732722693226 6.720680025287679E-5 0.0 1.8448925559613235E-5 4.7440094296148316E-5 16-17 0.008674948354209566 8.302016501825956E-5 0.0 1.8448925559613235E-5 5.2711215884609245E-5 18-19 0.008722388448505715 9.488018859229665E-5 0.0 1.8448925559613235E-5 5.4028996281724476E-5 20-21 0.008771146323198978 1.0278687097498803E-4 0.0 2.1084486353843697E-5 7.116014144422248E-5 22-23 0.008937186653235497 1.0805799256344895E-4 0.0 2.5037827545189392E-5 7.116014144422248E-5 24-25 0.009680414797208488 1.2387135732883173E-4 0.0 2.6355607942304623E-5 8.03846042240291E-5 26-27 0.01260852283959853 1.3046025931440789E-4 0.0 3.294450992788078E-5 9.224462779806618E-5 28-29 0.02167485197175132 1.4100250249132973E-4 0.0 3.426229032499601E-5 1.014690905778728E-4 30-31 0.044278739123468885 1.5154474566825158E-4 0.0 4.085119231057216E-5 1.1332911415190987E-4 32-33 0.09044190421481255 1.660403300365191E-4 0.0 4.744009429614832E-5 1.2518913772594696E-4 34-35 0.16910680502060627 1.858070359932476E-4 0.0 5.0075655090378784E-5 1.3177803971152312E-4 36-37 0.2900223809187085 1.9634927917016944E-4 0.0 5.4028996281724476E-5 1.4495584368267542E-4 38-39 0.45877074967129944 2.0030262036151514E-4 0.0 5.666455707595494E-5 1.5549808685959727E-4 40-41 0.6783986195565095 2.0425596155286083E-4 0.0 5.798233747307017E-5 1.607692084480582E-4 42-43 0.9524086508698708 2.1084486353843698E-4 0.0 6.0617898267300636E-5 1.7262923202209527E-4 44-45 1.2817180187677757 2.3456491068651113E-4 0.0 6.32534590615311E-5 1.8448925559613237E-4 46-47 1.6691797178099788 2.5564939704035485E-4 0.0 6.588901985576156E-5 1.9239593797882375E-4 48-49 2.1155593784072035 2.754161029970833E-4 0.0 6.588901985576156E-5 2.0820930274420652E-4 50-51 2.61925853293775 2.9386502855669654E-4 0.0 6.588901985576156E-5 2.1348042433266744E-4 52-53 3.1765425917561925 3.0704283252784884E-4 0.0 6.984236104710725E-5 2.292937890980502E-4 54-55 3.783608440636457 3.2549175808746207E-4 0.0 7.116014144422248E-5 2.3851825187785682E-4 56-57 4.442200820824325 3.399873424557296E-4 0.0 7.247792184133771E-5 2.4774271465766347E-4 58-59 5.149993849918887 3.4525846404419053E-4 0.0 7.774904342979863E-5 2.569671774374701E-4 60-61 5.89914541677691 3.518473660297667E-4 0.0 7.906682382691386E-5 2.661916402172767E-4 62-63 6.685101272345965 3.558007072211124E-4 0.0 8.302016501825956E-5 2.7278054220285285E-4 64-65 7.507040439438647 3.623896092066886E-4 0.0 8.433794541537479E-5 2.8068722458554423E-4 66-67 8.361142672683723 3.676607307951495E-4 0.0 8.565572581249002E-5 2.872761265711204E-4 68-69 9.244913413789448 3.795207543691866E-4 0.0 8.697350620960525E-5 2.9386502855669654E-4 70-71 10.15551548156084 3.9138077794322366E-4 0.0 8.960906700383571E-5 3.004539305422727E-4 72-73 11.08593828428524 4.0060524072303027E-4 0.0 9.224462779806618E-5 3.1626729530765546E-4 74-75 12.036755056435396 4.111474838999521E-4 0.0 9.224462779806618E-5 3.2022063649900115E-4 76-77 13.006313301393323 4.2564306826821965E-4 0.0 9.224462779806618E-5 3.320806600730382E-4 78-79 13.995228422604477 4.3750309184225673E-4 0.0 9.619796898941188E-5 3.4262290324996007E-4 80-81 14.997041978342596 4.480453350191786E-4 0.0 1.0542243176921849E-4 3.492118052355362E-4 82-83 16.01073400658031 4.6385869978456134E-4 0.0 1.0805799256344895E-4 3.5843626801534284E-4 84-85 17.037759336876036 4.783542841528289E-4 0.0 1.0805799256344895E-4 3.676607307951495E-4 86-87 18.072357951113986 4.928498685210965E-4 0.0 1.0937577296056418E-4 3.768851935749561E-4 88 18.852551153006456 4.954854293153269E-4 0.0 1.1069355335767941E-4 3.795207543691866E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 111100 0.0 78.41165 8 GTGGTAT 114790 0.0 76.066475 6 TGGTATC 114535 0.0 76.05182 7 AGTGGTA 115095 0.0 75.971146 5 GTATCAA 114645 0.0 75.823105 9 AAGCAGT 126090 0.0 70.10561 1 GCAGTGG 128255 0.0 68.63031 3 CAGTGGT 129160 0.0 67.964035 4 AGCAGTG 140985 0.0 62.77821 2 CAACGCA 105660 0.0 43.467945 12-13 ATCAACG 108025 0.0 42.585747 10-11 ACGCAGA 110245 0.0 41.69919 14-15 TCAACGC 105310 0.0 41.116127 12-13 AGAGTAC 114120 0.0 40.013035 18-19 AACGCAG 108315 0.0 39.90018 14-15 GAGTACT 107655 0.0 38.536045 20-21 CGCAGAG 112435 0.0 38.36991 16-17 TATCAAC 116000 0.0 37.50516 10-11 AGTACTT 119325 0.0 37.104458 20-21 GTACTTT 117825 0.0 35.123802 22-23 >>END_MODULE