FastQCFastQC Report
Thu 26 May 2016
SRR1660659_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660659_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43138574
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6189041.4346881285412911No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA968140.22442559181488012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA974950.073.407458
GTGGTAT997800.071.881156
GTATCAA1003300.071.1714559
AGTGGTA1012000.070.980565
TGGTATC1010550.070.7378857
AAGCAGT1144000.063.726031
CAGTGGT1162450.062.1342124
GCAGTGG1191350.061.0372853
AGCAGTG1330450.054.9704632
CAACGCA933350.040.72429312-13
ATCAACG959400.039.70770310-11
TCAACGC922400.038.76502612-13
ACGCAGA993850.038.30490514-15
AGAGTAC1001600.037.39481418-19
AACGCAG960350.037.1260714-15
GAGTACT891800.036.796220-21
CGCAGAG1018200.034.95265216-17
AGTACTT1002300.034.7532520-21
TATCAAC1040600.034.3694210-11
GTACTTT982000.033.34539822-23
Produced by FastQC (version 0.11.5)