FastQCFastQC Report
Thu 26 May 2016
SRR1660659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43138574
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16059953.722874566971082No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT483010.11196707614859963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2159150.081.64438
GTGGTAT2181800.080.9524466
AGTGGTA2191250.080.73495
TGGTATC2192950.080.36457
GTATCAA2196250.080.0276649
AAGCAGT2266550.078.894021
CAGTGGT2323500.076.392184
GCAGTGG2342200.076.119793
AGCAGTG2478700.072.24352
CAACGCA2123250.043.44539612-13
ATCAACG2148600.042.92403810-11
ACGCAGA2177800.042.39024714-15
AGAGTAC2203100.041.63466318-19
TCAACGC2115100.041.4291112-13
AACGCAG2148900.040.67101314-15
AGTACTT2196950.040.4804420-21
GAGTACT2088700.040.21505420-21
TATCAAC2225150.039.46457710-11
CGCAGAG2210350.039.4642716-17
GTACTTT2178300.038.4752422-23
Produced by FastQC (version 0.11.5)