##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660659_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43138574 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5227090955765 34.0 31.0 34.0 31.0 34.0 2 32.73828281852803 34.0 31.0 34.0 31.0 34.0 3 32.793370429908045 34.0 31.0 34.0 31.0 34.0 4 36.18879963440609 37.0 37.0 37.0 35.0 37.0 5 36.12929961477169 37.0 37.0 37.0 35.0 37.0 6 36.123488203388455 37.0 36.0 37.0 35.0 37.0 7 36.07139579996316 37.0 35.0 37.0 35.0 37.0 8 36.09611080329174 37.0 36.0 37.0 35.0 37.0 9 37.82980825930871 39.0 38.0 39.0 35.0 39.0 10-11 37.73876230818385 39.0 37.5 39.0 35.0 39.0 12-13 37.81452679682921 39.0 38.0 39.0 35.0 39.0 14-15 39.265478235326 41.0 39.0 41.0 36.0 41.0 16-17 39.228421157361396 41.0 39.0 41.0 36.0 41.0 18-19 39.1981928517155 40.0 39.0 41.0 36.0 41.0 20-21 39.15381309312635 40.0 39.0 41.0 36.0 41.0 22-23 39.030243002469206 40.0 38.5 41.0 35.5 41.0 24-25 39.026113508063574 40.0 39.0 41.0 35.0 41.0 26-27 38.98650442177342 40.0 38.5 41.0 35.0 41.0 28-29 38.919722091416375 40.0 38.0 41.0 35.0 41.0 30-31 38.83780367890696 40.0 38.0 41.0 35.0 41.0 32-33 38.75129004032446 40.0 38.0 41.0 35.0 41.0 34-35 38.64193758467769 40.0 38.0 41.0 35.0 41.0 36-37 38.49961759283003 40.0 38.0 41.0 34.0 41.0 38-39 38.19641003895956 40.0 37.0 41.0 34.0 41.0 40-41 38.064515414904534 40.0 37.0 41.0 33.5 41.0 42-43 37.931369451387056 40.0 37.0 41.0 33.0 41.0 44-45 37.79226688160809 40.0 36.5 41.0 33.0 41.0 46-47 37.64172758700832 40.0 36.0 41.0 33.0 41.0 48-49 37.47441286538586 39.0 36.0 41.0 33.0 41.0 50-51 37.36497924108478 39.0 36.0 41.0 32.5 41.0 52-53 37.12426235971546 39.0 35.0 41.0 31.5 41.0 54-55 36.62947595347032 39.0 35.0 41.0 30.5 41.0 56-57 36.22419550771428 39.0 35.0 41.0 29.5 41.0 58-59 35.97697462136787 38.5 35.0 40.0 29.0 41.0 60-61 35.717588961100105 38.0 35.0 40.0 28.0 41.0 62-63 35.42888513190074 37.5 34.0 40.0 28.0 41.0 64-65 35.408903537237926 37.0 34.0 40.0 29.0 41.0 66-67 35.197380179975355 37.0 34.0 39.5 29.0 41.0 68-69 34.915616577868335 36.0 34.0 39.0 29.0 41.0 70-71 34.59974215883909 36.0 34.0 39.0 28.0 40.5 72-73 34.23755182542659 35.0 34.0 38.0 28.0 40.0 74-75 33.867315514416404 35.0 33.5 37.0 27.5 39.5 76-77 32.89848994544882 35.0 32.5 36.0 26.0 39.0 78-79 33.15720785068139 35.0 33.0 36.0 26.0 39.0 80-81 32.96060587213662 35.0 33.0 36.0 26.0 37.5 82-83 32.7100214879611 35.0 33.0 35.5 26.0 37.0 84-85 32.46304710953125 35.0 33.0 35.0 25.5 37.0 86-87 32.25574391958344 35.0 32.5 35.0 25.0 36.0 88-89 32.070944730347364 35.0 32.0 35.0 25.0 36.0 90-91 31.916496045975002 35.0 32.0 35.0 25.0 36.0 92-93 31.772033146019155 35.0 32.0 35.0 24.0 35.0 94-95 31.6272644640502 35.0 32.0 35.0 24.0 35.0 96-97 31.52049681799867 35.0 32.0 35.0 23.5 35.0 98-99 31.415422517211624 35.0 31.5 35.0 23.0 35.0 100 31.327507534208248 35.0 31.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.006940099672533506 1101 2 0.022359374508859275 1101 3 0.06055374462526686 1101 4 0.09958007496241095 1101 5 0.0891486604109133 1101 6 0.07731419936592943 1101 7 0.09784519876317432 1101 8 0.10086671850696405 1101 9 0.103055256081511 1101 10-11 0.07069256563946169 1101 12-13 0.10363301162380623 1101 14-15 0.12798902798120082 1101 16-17 0.1377960796333184 1101 18-19 0.143373278187525 1101 20-21 0.13412502709905283 1101 22-23 0.1686587809591984 1101 24-25 0.17711118600013975 1101 26-27 0.20525022300306262 1101 28-29 0.2233101385061147 1101 30-31 0.24532673000028637 1101 32-33 0.2891573378251593 1101 34-35 0.311460624768614 1101 36-37 0.3435434396203192 1101 38-39 0.35706422861068887 1101 40-41 0.3937055430449661 1101 42-43 0.4327815916913309 1101 44-45 0.388600988194149 1101 46-47 0.42410100576901044 1101 48-49 0.46075226239564415 1101 50-51 0.4023956116508032 1101 52-53 0.41016051736787773 1101 54-55 0.4094621155046383 1101 56-57 0.4070888620557511 1101 58-59 0.41021691083610534 1101 60-61 0.44682351175630686 1101 62-63 0.4821869758362922 1101 64-65 0.35076601359373427 1101 66-67 0.3013662242364532 1101 68-69 0.34647746447736694 1101 70-71 0.3999087454554129 1101 72-73 0.4112667378844108 1101 74-75 0.41430401633498803 1101 76-77 0.8110054190850136 1101 78-79 0.45565684630083325 1101 80-81 0.37656212063912164 1101 82-83 0.3872661149920802 1101 84-85 0.41451896074559613 1101 86-87 0.3745955163305297 1101 88-89 0.4305313539244082 1101 90-91 0.4679378240642791 1101 92-93 0.49156080717287765 1101 94-95 0.4842182482425734 1101 96-97 0.483186623346878 1101 98-99 0.5179490516882517 1101 100 0.5575290393327137 1102 1 -0.011751159276812473 1102 2 0.01655980312031602 1102 3 0.0354087005255721 1102 4 0.0841660152407755 1102 5 0.04844338699962947 1102 6 0.07587617791689638 1102 7 0.0605282976491992 1102 8 0.07596141334936135 1102 9 0.07064195128595685 1102 10-11 0.08431423661584603 1102 12-13 0.06962408885133442 1102 14-15 0.09296684077216355 1102 16-17 0.11139914122946948 1102 18-19 0.10263799439964316 1102 20-21 0.11882129101501704 1102 22-23 0.14645609577011243 1102 24-25 0.12247047508754605 1102 26-27 0.16522643190919695 1102 28-29 0.22928556580449566 1102 30-31 0.24145099429691896 1102 32-33 0.22619198563041465 1102 34-35 0.2530961739500839 1102 36-37 0.28558975303702283 1102 38-39 0.3373848301516418 1102 40-41 0.32852364317089666 1102 42-43 0.3314309666335049 1102 44-45 0.40277044914589055 1102 46-47 0.43036774748715345 1102 48-49 0.4705913731279523 1102 50-51 0.4325061911867465 1102 52-53 0.41213889486321875 1102 54-55 0.42360796013171864 1102 56-57 0.4356714616168844 1102 58-59 0.4234075821307073 1102 60-61 0.38469915804898136 1102 62-63 0.4272314987626018 1102 64-65 0.3310813408030171 1102 66-67 0.2969872124947628 1102 68-69 0.3303394931525432 1102 70-71 0.3192985616438122 1102 72-73 0.35801524083885994 1102 74-75 0.359691704032052 1102 76-77 0.7550143911175056 1102 78-79 0.41347580735047984 1102 80-81 0.3567383813175198 1102 82-83 0.3598219924417876 1102 84-85 0.41116818222030815 1102 86-87 0.4213197253794476 1102 88-89 0.4581524463560953 1102 90-91 0.5030726871964077 1102 92-93 0.4975471559488618 1102 94-95 0.5586854410355677 1102 96-97 0.57499211033657 1102 98-99 0.588813958560177 1102 100 0.5724556619796815 1103 1 -0.049943403762490846 1103 2 -0.03543839479161903 1103 3 -0.010548283237909573 1103 4 0.0404770989099319 1103 5 0.018016734993757666 1103 6 0.038627963244287855 1103 7 0.03327894495308925 1103 8 0.03910711222834351 1103 9 0.05502820194493552 1103 10-11 0.015147102153648007 1103 12-13 0.040890103765441665 1103 14-15 0.07557754389819138 1103 16-17 0.05277063078382582 1103 18-19 0.026738869206781146 1103 20-21 0.07794922067656529 1103 22-23 0.08441463379422487 1103 24-25 0.11188630025680624 1103 26-27 0.11754944559067582 1103 28-29 0.1691514065372175 1103 30-31 0.12449501077325209 1103 32-33 0.1296637893216115 1103 34-35 0.21294129005245566 1103 36-37 0.24409440195337595 1103 38-39 0.2747710425275116 1103 40-41 0.24514545521530806 1103 42-43 0.2804159455089348 1103 44-45 0.32245217908417345 1103 46-47 0.34555317185482437 1103 48-49 0.3592673609114456 1103 50-51 0.38839042939591906 1103 52-53 0.33738709777756526 1103 54-55 0.35590252835613256 1103 56-57 0.32615387866406564 1103 58-59 0.3711992591024398 1103 60-61 0.39933576493607603 1103 62-63 0.4023993656323839 1103 64-65 0.31528732198238885 1103 66-67 0.22914207753670013 1103 68-69 0.23321371770300203 1103 70-71 0.28109551804134014 1103 72-73 0.3217869969882159 1103 74-75 0.3380925430491786 1103 76-77 0.7034696724796419 1103 78-79 0.38284378013486275 1103 80-81 0.3340875306629414 1103 82-83 0.3361267795204057 1103 84-85 0.3036082242078493 1103 86-87 0.37471808216982083 1103 88-89 0.4262832826774172 1103 90-91 0.42270945073573074 1103 92-93 0.42277206025672953 1103 94-95 0.44064832211838834 1103 96-97 0.48446556987973466 1103 98-99 0.5544771503538293 1103 100 0.5187054648295089 1104 1 -0.048832836506186084 1104 2 -0.0158358291830325 1104 3 0.00346769364209365 1104 4 0.04089561660284602 1104 5 0.023887441544005128 1104 6 0.0446925938859124 1104 7 0.03710258370229269 1104 8 0.020405995145672762 1104 9 0.032991444892566335 1104 10-11 -0.013529593882552149 1104 12-13 0.008970883801190155 1104 14-15 0.038810216857626756 1104 16-17 0.056081638885075336 1104 18-19 0.0562726663618065 1104 20-21 0.06858522728504823 1104 22-23 0.0691873373862748 1104 24-25 0.10016240358185513 1104 26-27 0.11291946971739009 1104 28-29 0.11796490721978614 1104 30-31 0.13287689996023033 1104 32-33 0.17377404354672166 1104 34-35 0.19929568173168377 1104 36-37 0.22613474058718452 1104 38-39 0.2541473242248813 1104 40-41 0.2520124692543604 1104 42-43 0.314901820081289 1104 44-45 0.3336187072575072 1104 46-47 0.3555192677680523 1104 48-49 0.387628843517291 1104 50-51 0.3418448392221549 1104 52-53 0.35075140478218714 1104 54-55 0.34670344697902067 1104 56-57 0.35704168851788864 1104 58-59 0.3521728452159607 1104 60-61 0.36632343989641925 1104 62-63 0.3838927160460912 1104 64-65 0.3014347566672555 1104 66-67 0.259146445600571 1104 68-69 0.24593860013340674 1104 70-71 0.25803097856218216 1104 72-73 0.25584464928677875 1104 74-75 0.32991259044600696 1104 76-77 0.7063227918056683 1104 78-79 0.409371850287215 1104 80-81 0.3245664606396801 1104 82-83 0.342001807050913 1104 84-85 0.32977668253659687 1104 86-87 0.35030156292078374 1104 88-89 0.4083950205120601 1104 90-91 0.44135493694659544 1104 92-93 0.4521232911558606 1104 94-95 0.4832739212225938 1104 96-97 0.5189257012389312 1104 98-99 0.5393455051108553 1104 100 0.5425092269851746 1105 1 -0.09829938854338849 1105 2 -0.06942998549252621 1105 3 -0.05639094930274524 1105 4 0.021633039613142557 1105 5 -0.026439708573384735 1105 6 -0.07600600807698754 1105 7 -0.018390797823734317 1105 8 -0.009135322146953229 1105 9 -0.027464278395804342 1105 10-11 -0.014292690174158906 1105 12-13 -0.021058167903383662 1105 14-15 -0.008987712680323057 1105 16-17 -0.004798486069589103 1105 18-19 0.009064461079873354 1105 20-21 0.020661057675994243 1105 22-23 0.027378251177815116 1105 24-25 0.02728160961729742 1105 26-27 0.047274063504239905 1105 28-29 0.07656959865578727 1105 30-31 0.1019342563800123 1105 32-33 0.11239322144081854 1105 34-35 0.13388770282148954 1105 36-37 0.16527034469669388 1105 38-39 0.19865283522108257 1105 40-41 0.19796047277726814 1105 42-43 0.20230520831677268 1105 44-45 0.2648162871163109 1105 46-47 0.2984523812461717 1105 48-49 0.3384969652101404 1105 50-51 0.29264610159268045 1105 52-53 0.2898774374841153 1105 54-55 0.28882830309589735 1105 56-57 0.25771596644131733 1105 58-59 0.27589990572192136 1105 60-61 0.30106385188859264 1105 62-63 0.3158132138654679 1105 64-65 0.24456044715052627 1105 66-67 0.1440949896116308 1105 68-69 0.17405470931723954 1105 70-71 0.2160347595970009 1105 72-73 0.25647381513498146 1105 74-75 0.29369910601623417 1105 76-77 0.6241086979663848 1105 78-79 0.3497300398604466 1105 80-81 0.3022721687285781 1105 82-83 0.3075404138751807 1105 84-85 0.3342544233053957 1105 86-87 0.3767146286276528 1105 88-89 0.38306193026924973 1105 90-91 0.38314848733444506 1105 92-93 0.4417750509726126 1105 94-95 0.4347606682218874 1105 96-97 0.4957956026368926 1105 98-99 0.5065829243220712 1105 100 0.480991143071293 1106 1 -0.12487565148190072 1106 2 -0.0735343797336725 1106 3 -0.039687665333751454 1106 4 0.004514820627058214 1106 5 -8.799549091662584E-4 1106 6 0.01993347824596725 1106 7 -0.012862976350220379 1106 8 -0.02996021451855313 1106 9 -0.03914692671858688 1106 10-11 -0.019566068271622328 1106 12-13 -0.015659320629289653 1106 14-15 -0.05727665060932452 1106 16-17 -0.00817178840361521 1106 18-19 -0.0025483932613923344 1106 20-21 -0.007085505651836854 1106 22-23 0.0016747131240393287 1106 24-25 0.031905547900343834 1106 26-27 0.07893021090988839 1106 28-29 0.07282096888631173 1106 30-31 0.10584286998494008 1106 32-33 0.12086490848601272 1106 34-35 0.1170518364570654 1106 36-37 0.13274115178126777 1106 38-39 0.15917597242005854 1106 40-41 0.20462599208870813 1106 42-43 0.2004812718656268 1106 44-45 0.28396034999396846 1106 46-47 0.2950482523369047 1106 48-49 0.3250398258330307 1106 50-51 0.2854639731227451 1106 52-53 0.25705139056000803 1106 54-55 0.24441275766318427 1106 56-57 0.26498926652265453 1106 58-59 0.2807416213102414 1106 60-61 0.31162535092448707 1106 62-63 0.322771520621167 1106 64-65 0.2183755962773617 1106 66-67 0.14542504689118374 1106 68-69 0.18078771822966644 1106 70-71 0.18893060615565105 1106 72-73 0.24362336013340524 1106 74-75 0.2513314278330654 1106 76-77 0.6110405786843316 1106 78-79 0.3259349750802869 1106 80-81 0.28395416689541975 1106 82-83 0.272019756003381 1106 84-85 0.29330417774104234 1106 86-87 0.33627961657914796 1106 88-89 0.3536081075550612 1106 90-91 0.3623973688146407 1106 92-93 0.3846118409563992 1106 94-95 0.4115638247491269 1106 96-97 0.43402738393264073 1106 98-99 0.4904687089798152 1106 100 0.4884675609078073 1107 1 -0.12797288744305746 1107 2 -0.08148806097302241 1107 3 -0.08309571984298003 1107 4 -0.010800803158183214 1107 5 -0.026397280875919193 1107 6 -0.00815528396192633 1107 7 -0.0472096043178567 1107 8 -0.04440088792386376 1107 9 -0.06884460373572665 1107 10-11 -0.060310042395947505 1107 12-13 -0.07557167019039213 1107 14-15 -0.08140291107110897 1107 16-17 -0.08386403292027467 1107 18-19 -0.025528190376860493 1107 20-21 -0.02566831449903617 1107 22-23 -0.01461262599820401 1107 24-25 0.017251307814255767 1107 26-27 -0.025327423815028283 1107 28-29 0.008340442521522107 1107 30-31 0.06669539686439663 1107 32-33 0.057140380437274985 1107 34-35 0.07279779454279378 1107 36-37 0.07227020954958618 1107 38-39 0.09994897515495182 1107 40-41 0.14747198585957477 1107 42-43 0.17953609941189086 1107 44-45 0.19342626092122117 1107 46-47 0.24028576587970463 1107 48-49 0.27062821647876234 1107 50-51 0.226406324174512 1107 52-53 0.25535341895502484 1107 54-55 0.26379623285792775 1107 56-57 0.22363220369852144 1107 58-59 0.27960529345364904 1107 60-61 0.27797468968675076 1107 62-63 0.3314633554736517 1107 64-65 0.2359070881073393 1107 66-67 0.12712420012790204 1107 68-69 0.12325884666615394 1107 70-71 0.16707535280122698 1107 72-73 0.23274418684722065 1107 74-75 0.25110013110145957 1107 76-77 0.6207270287870088 1107 78-79 0.3087181636031673 1107 80-81 0.281335111120498 1107 82-83 0.29265278692918173 1107 84-85 0.32134872403935333 1107 86-87 0.2845187050467999 1107 88-89 0.29961070813362056 1107 90-91 0.35003216636638257 1107 92-93 0.42123340347889027 1107 94-95 0.4308002910773219 1107 96-97 0.43971555820913366 1107 98-99 0.47524657485363875 1107 100 0.4254951691178981 1108 1 -0.13977788500507415 1108 2 -0.10951737804287376 1108 3 -0.09322590487403914 1108 4 -0.042187834838799176 1108 5 -0.04036834770251119 1108 6 -0.033851733339552936 1108 7 -0.04679729150348777 1108 8 -0.09701327417939609 1108 9 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0.47507611594573973 2316 64-65 0.2581113310995775 2316 66-67 0.17564540885693702 2316 68-69 0.13964163533987772 2316 70-71 0.12093181311451673 2316 72-73 0.07452089185720467 2316 74-75 0.11071458179273463 2316 76-77 -0.6065192184768691 2316 78-79 -0.09299723385330338 2316 80-81 0.05622237123936458 2316 82-83 0.10607173030800965 2316 84-85 0.10975023182746213 2316 86-87 0.13713495247558427 2316 88-89 0.1039066990823585 2316 90-91 0.19297170546565212 2316 92-93 0.3258001495801288 2316 94-95 0.33539150747498425 2316 96-97 0.31680435750323355 2316 98-99 -0.44101606839202745 2316 100 0.056049955490550474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 14.0 7 202.0 8 347.0 9 142.0 10 40.0 11 57.0 12 148.0 13 648.0 14 2137.0 15 5764.0 16 11790.0 17 19514.0 18 27556.0 19 39486.0 20 59912.0 21 90899.0 22 141916.0 23 232773.0 24 386817.0 25 550132.0 26 614216.0 27 579603.0 28 562951.0 29 622904.0 30 743801.0 31 920300.0 32 1175781.0 33 1571818.0 34 2236313.0 35 3408547.0 36 5576769.0 37 9941998.0 38 1.1083949E7 39 2525365.0 40 3965.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.160621465192744 30.63272901830928 28.136744133468696 24.069905383029283 2 22.44242855135092 31.91458827304448 25.273418251090142 20.369564924514464 3 25.49393728221058 24.800441943213052 26.445732768078983 23.259888006497388 4 21.0477959312593 27.061583237872522 23.95271408552134 27.937906745346837 5 23.131003290899717 30.06722656503197 25.741776158544788 21.05999398552353 6 28.03858614373233 26.729299156413767 24.804811482299606 20.4273032175543 7 22.43611483309578 25.66793005257893 29.67662537941101 22.21932973491428 8 26.741681354603884 26.76396071877573 24.58613954184021 21.908218384780174 9 25.795141026219365 24.659834606493945 27.13221813961676 22.412806227669925 10-11 21.9624610215442 28.043227622684054 27.683427829580086 22.310883526191663 12-13 21.91342833910087 26.11925581962909 27.626991100818493 24.34032474045155 14-15 21.99976869889116 30.27262908597767 25.534399908536614 22.193202306594557 16-17 24.287870526271917 25.728630946400777 25.645960156216567 24.337538371110735 18-19 22.031412599391885 27.918812279100408 25.702964277737077 24.34681084377063 20-21 24.235664953446733 28.017156222900663 25.671217389362155 22.075961434290445 22-23 24.162406813934602 25.862492102168588 27.787215870473155 22.18788521342365 24-25 22.181934429660718 27.673445041724527 25.63844196747992 24.506178561134835 26-27 22.250545875526576 25.589816160734518 29.974609090137445 22.18502887360146 28-29 22.308834057110307 25.63668739624749 29.922071139685556 22.13240740695665 30-31 22.213062290856026 25.54834174255833 29.982625185020336 22.25597078156531 32-33 22.326309694953988 25.706358367888555 29.762457111904016 22.20487482525344 34-35 22.341063093855425 25.823374796467892 29.656239793113308 22.17932231656338 36-37 22.261417956090646 25.778993815443062 29.758016149566036 22.20157207890026 38-39 22.29922540279896 25.76847413895606 29.78661351767639 22.14568694056859 40-41 22.325646706041894 25.82286025974639 29.735421975012066 22.11607105919965 42-43 22.33433424860888 25.84848301418084 29.700196969053806 22.116985768156475 44-45 22.421976378977075 25.915733748615004 29.59887343347724 22.063416438930677 46-47 22.4477025660768 26.057771403908742 29.459620248888918 22.03490578112554 48-49 22.5031877235236 26.0970856968741 29.34503584659623 22.054690733006073 50-51 22.582996442116237 26.20794392886282 29.203106786194923 22.005952842826026 52-53 22.676687324769652 26.36346766009423 28.9191258898004 22.04071912533571 54-55 22.925551966016634 26.619515759660136 28.214164006517294 22.240768267805937 56-57 23.271169429824845 27.05575261307634 27.30640121417834 22.366676742920475 58-59 23.30647439475608 27.372194215075268 26.93656845623674 22.384762933931913 60-61 23.248888183433838 27.500432423688327 26.808911734374924 22.44176765850291 62-63 23.233616958800347 27.606525710204448 26.710183135765952 22.449674195229253 64-65 23.244813021878667 27.655242968578513 26.65190979029758 22.448034219245237 66-67 23.164644564052363 27.524702960742424 26.76657883324745 22.544073641957755 68-69 23.14452645037706 27.451868650155237 26.847966835174407 22.5556380642933 70-71 23.11259128913855 27.401294127056776 26.90463817879672 22.581476405007955 72-73 23.092965666863652 27.36212501590754 26.89394894002636 22.650960377202452 74-75 23.133435590769224 27.39670545556747 26.78610399783623 22.683754955827077 76-77 23.20188292460709 27.459047490989054 26.577245521475877 22.761824062927978 78-79 23.250338489023022 27.524592580116604 26.365121404845503 22.859947526014867 80-81 23.320320339278876 27.632512902628985 26.137704681805623 22.909462076286516 82-83 23.312646871098163 27.707668661016367 25.94968776277976 23.02999670510571 84-85 23.331052238463432 27.670761046503273 25.859403651328154 23.138783063705137 86-87 23.341528746806464 27.63228362750559 25.82879850529493 23.19738912039302 88-89 23.380459528622602 27.538767640534935 25.78587337540354 23.294899455438927 90-91 23.429607069587522 27.441578445746025 25.760962416913596 23.36785206775286 92-93 23.467957159067126 27.39034605580174 25.761590234523908 23.380106550607227 94-95 23.501843910274353 27.29623652679793 25.779767673216703 23.422151889711017 96-97 23.47486670967267 27.1774338842979 25.837702550344982 23.50999685568445 98-99 23.474107406968635 27.13772280133532 25.847323445747417 23.540846345948626 100 23.3755730600872 27.135031759308664 25.8707376826825 23.618657497921635 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 46108.0 1 31051.5 2 11981.0 3 7138.5 4 5974.5 5 5485.0 6 5450.5 7 5980.5 8 6113.5 9 6251.0 10 6909.5 11 13073.5 12 152650.5 13 176757.0 14 62562.0 15 61048.0 16 68575.5 17 77470.0 18 86667.5 19 95722.5 20 107162.0 21 124895.5 22 148111.0 23 174366.0 24 203827.0 25 238100.5 26 274956.0 27 319321.5 28 373297.0 29 429962.5 30 482010.5 31 535459.0 32 594156.0 33 647844.5 34 699212.5 35 763769.5 36 838379.0 37 901634.5 38 973439.5 39 1048759.5 40 1107898.0 41 1182144.5 42 1268438.0 43 1315192.0 44 1346239.0 45 1402783.0 46 1460461.5 47 1506029.0 48 1552292.0 49 1602107.5 50 1647915.5 51 1687051.5 52 1704292.5 53 1683498.0 54 1633686.0 55 1564883.0 56 1476098.0 57 1358293.5 58 1207567.0 59 1033062.5 60 846236.5 61 674716.5 62 528298.0 63 398505.5 64 294413.5 65 217926.5 66 158346.5 67 116142.5 68 86421.0 69 65407.0 70 50921.5 71 40685.5 72 33916.5 73 27155.5 74 21044.0 75 17217.5 76 12998.5 77 7900.5 78 4653.5 79 2832.5 80 1635.0 81 1078.0 82 681.5 83 389.0 84 222.0 85 118.0 86 71.5 87 39.0 88 24.5 89 19.5 90 12.0 91 8.0 92 7.5 93 5.5 94 2.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17397886170275356 2 0.03050865798206496 3 0.0 4 0.14270290900204535 5 0.006142993971010725 6 0.003317216744345791 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 1.286551567513567E-4 20-21 9.446302049761774E-4 22-23 3.477166398685316E-4 24-25 7.754081069068254E-4 26-27 0.0038932673110613253 28-29 0.0038642909244056144 30-31 0.003300989967818593 32-33 0.0033809647949883553 34-35 0.00310279148309353 36-37 0.004485544654304057 38-39 0.005741960779695685 40-41 0.007129350172771125 42-43 0.01016955266068832 44-45 0.0059123419332312655 46-47 0.00794416616552972 48-49 0.0073426163785571584 50-51 0.009794018689630308 52-53 0.011158226973381178 54-55 0.046519850192544614 56-57 0.04414494554224254 58-59 0.04456104645461855 60-61 0.06240586441267159 62-63 0.040578531872657635 64-65 0.030988506945083535 66-67 0.0345328985608101 68-69 0.03210235924812906 70-71 0.024174419859126543 72-73 0.015093220281226727 74-75 0.010729376450876656 76-77 0.008226975699289457 78-79 0.008962975920344516 80-81 0.003326489188075619 82-83 0.0014360697226570354 84-85 0.003231446639844887 86-87 0.002861707946118015 88-89 0.003780838930837167 90-91 0.002729575622967973 92-93 0.0026380102411359264 94-95 0.0014580917765153758 96-97 9.504254823073196E-4 98-99 7.869986615691097E-4 100 0.0030135442121939403 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.3138574E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.13739231329962 #Duplication Level Percentage of deduplicated Percentage of total 1 75.3724625644584 32.513714872625314 2 11.345493739810614 9.788300288845907 3 4.387601448930878 5.678090550507994 4 2.420026151610607 4.175744700418859 5 1.4478170205254695 3.1227525406139875 6 0.9854165811460612 2.5504980991756856 7 0.685110969362773 2.0687730464482854 8 0.5058889234919096 1.7458183167698655 9 0.3866743167857422 1.5012109530597293 >10 2.168776084770962 17.006342834408024 >50 0.16384726036105987 5.0233681303308995 >100 0.12767670510665197 9.631752531612172 >500 0.002712194356588018 0.7293686710363569 >1k 4.372289345186883E-4 0.36914732987873083 >5k 3.2078378902618E-5 0.09629937794007612 >10k+ 2.673196877970392E-5 3.998817756328015 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1605995 3.722874566971082 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 48301 0.11196707614859963 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.003004271768464113 1.622677652719814E-5 0.0 4.636221864913754E-6 4.636221864913754E-6 2 0.0030436796543158798 2.3181109324568774E-5 0.0 9.272443729827508E-6 4.636221864913754E-6 3 0.0030807694292351896 4.172599678422379E-5 0.0 9.272443729827508E-6 6.954332797370632E-6 4 0.0031201773150869565 4.636221864913755E-5 0.0 9.272443729827508E-6 9.272443729827508E-6 5 0.0032198560851826024 5.5634662378965055E-5 0.0 9.272443729827508E-6 1.622677652719814E-5 6 0.0032337647507773436 6.0270884243878806E-5 0.0 1.1590554662284387E-5 2.7817331189482528E-5 7 0.0032569458601019127 6.490710610879256E-5 0.0 1.3908665594741264E-5 3.477166398685316E-5 8 0.003268536414764197 7.417954983862006E-5 0.0 1.622677652719814E-5 3.708977491931003E-5 9 0.003321852966210705 7.881577170353382E-5 0.0 1.622677652719814E-5 4.404410771668067E-5 10-11 0.003491075064280057 8.69291599671329E-5 0.0 1.8544887459655016E-5 4.752127411536598E-5 12-13 0.003631320775693698 9.851971462941728E-5 0.0 1.8544887459655016E-5 5.215749598027974E-5 14-15 0.0037055003255323186 1.077921583592448E-4 0.0 1.9703942925883455E-5 6.490710610879256E-5 16-17 0.0038457460369459595 1.1126932475793011E-4 0.0 2.549922025702565E-5 7.070238343993477E-5 18-19 0.004005695691285484 1.1822365755530075E-4 0.0 2.549922025702565E-5 7.881577170353382E-5 20-21 0.004087988629387702 1.4024571141364105E-4 0.0 3.0135442121939403E-5 8.577010450090446E-5 22-23 0.004330231221829447 1.6574493167066673E-4 0.0 3.59307194530816E-5 9.736065916318885E-5 24-25 0.005287611036934137 1.7501737540049424E-4 0.0 3.708977491931003E-5 1.0663310289301635E-4 26-27 0.008829684541728245 1.854488745965502E-4 0.0 3.708977491931003E-5 1.1011026929170166E-4 28-29 0.019936913074595372 2.0283470658997676E-4 0.0 3.940788585176691E-5 1.1938271302152918E-4 30-31 0.046566212411193755 2.1442526125226114E-4 0.0 4.056694131799535E-5 1.3097326768381356E-4 32-33 0.099005358869767 2.2485676044831709E-4 0.0 4.520316318290911E-5 1.3908665594741264E-4 34-35 0.18880549922674772 2.45719758840429E-4 0.0 4.636221864913755E-5 1.472000442110117E-4 36-37 0.3228189693984785 2.561512580364849E-4 0.0 5.4475606912736616E-5 1.6226776527198143E-4 38-39 0.5080406691236479 2.700599236312262E-4 0.0 5.5634662378965055E-5 1.7154020900180892E-4 40-41 0.7478469269753794 2.7237803456368307E-4 0.0 5.5634662378965055E-5 1.8660793006277862E-4 42-43 1.0411354812052898 2.8512764469219593E-4 0.0 5.5634662378965055E-5 2.0051659565751987E-4 44-45 1.3923142197514458 3.025134766856225E-4 0.0 5.5634662378965055E-5 2.0631187298866208E-4 46-47 1.8038646803670422 3.1758119774659215E-4 0.0 5.6793717845193494E-5 2.0978903938734738E-4 48-49 2.2744041098808694 3.4076230707116097E-4 0.0 6.0270884243878806E-5 2.2137959404963176E-4 50-51 2.7996312534577523 3.6510247186195816E-4 0.0 6.142993971010725E-5 2.341292041781446E-4 52-53 3.374405700104969 3.7669302652424254E-4 0.0 6.490710610879256E-5 2.4108353697551522E-4 54-55 3.997963632270274 3.96396969450126E-4 0.0 7.070238343993477E-5 2.5151503617157117E-4 56-57 4.671786322839508 4.172599678422379E-4 0.0 7.302049437239163E-5 2.5962842443517025E-4 58-59 5.390340441016896 4.381229662343498E-4 0.0 7.417954983862006E-5 2.6890086816499777E-4 60-61 6.145622708808131 4.555087982277764E-4 0.0 7.765671623730539E-5 2.770142564285968E-4 62-63 6.935757774468854 4.6941746382251763E-4 0.0 8.11338826359907E-5 2.8860481109088123E-4 64-65 7.75778541033832 4.8448518488348737E-4 0.0 8.577010450090446E-5 3.036725321518509E-4 66-67 8.607090257550006 5.007119614106855E-4 0.0 8.924727089958977E-5 3.094678094829931E-4 68-69 9.485701822225277 5.076662942080561E-4 0.0 9.040632636581822E-5 3.268536414764197E-4 70-71 10.38367077224203 5.146206270054267E-4 0.0 9.040632636581822E-5 3.3496702974001875E-4 72-73 11.304395458227248 5.28529292600168E-4 0.0 9.040632636581822E-5 3.4655758440230313E-4 74-75 12.242574360478397 5.435970136611377E-4 0.0 9.156538183204666E-5 3.4887569533476005E-4 76-77 13.198194729385353 5.598237901883359E-4 0.0 9.504254823073197E-5 3.535119171996738E-4 78-79 14.168131751411162 5.806867885804478E-4 0.0 9.62016036969604E-5 3.5930719453081594E-4 80-81 15.148298596981903 5.922773432427322E-4 0.0 9.851971462941728E-5 3.639434163957297E-4 82-83 16.14112232824386 6.05026953371245E-4 0.0 1.019968810281026E-4 3.7669302652424254E-4 84-85 17.144499259525823 6.224127853646715E-4 0.0 1.054740474267879E-4 3.9755602491635443E-4 86-87 18.15555956949342 6.351623954931843E-4 0.0 1.0663310289301635E-4 4.045103577137251E-4 88 18.91407907920183 6.490710610879256E-4 0.0 1.0895121382547323E-4 4.1262374597732417E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 215915 0.0 81.6443 8 GTGGTAT 218180 0.0 80.952446 6 AGTGGTA 219125 0.0 80.7349 5 TGGTATC 219295 0.0 80.3645 7 GTATCAA 219625 0.0 80.027664 9 AAGCAGT 226655 0.0 78.89402 1 CAGTGGT 232350 0.0 76.39218 4 GCAGTGG 234220 0.0 76.11979 3 AGCAGTG 247870 0.0 72.2435 2 CAACGCA 212325 0.0 43.445396 12-13 ATCAACG 214860 0.0 42.924038 10-11 ACGCAGA 217780 0.0 42.390247 14-15 AGAGTAC 220310 0.0 41.634663 18-19 TCAACGC 211510 0.0 41.42911 12-13 AACGCAG 214890 0.0 40.671013 14-15 AGTACTT 219695 0.0 40.48044 20-21 GAGTACT 208870 0.0 40.215054 20-21 TATCAAC 222515 0.0 39.464577 10-11 CGCAGAG 221035 0.0 39.46427 16-17 GTACTTT 217830 0.0 38.47524 22-23 >>END_MODULE