FastQCFastQC Report
Thu 26 May 2016
SRR1660658_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660658_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28032408
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12166604.340190824848154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA403720.14401902255418086No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT324940.11591583569987994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1617250.082.867358
GTGGTAT1627650.082.3987356
AGTGGTA1642350.081.794015
TGGTATC1639500.081.566667
GTATCAA1644150.081.300349
AAGCAGT1676600.081.090381
GCAGTGG1731150.078.426583
CAGTGGT1743100.077.3332064
AGCAGTG1781100.076.507962
CAACGCA1595100.044.0157512-13
ATCAACG1616400.043.46486710-11
ACGCAGA1635050.042.95343814-15
AGAGTAC1638600.042.30237218-19
TCAACGC1593750.041.98784312-13
AACGCAG1618550.041.1965714-15
AGTACTT1636200.041.16217420-21
GAGTACT1555450.040.98201820-21
CGCAGAG1652000.040.25245316-17
TATCAAC1660350.040.23852510-11
GTACTTT1607300.039.54479622-23
Produced by FastQC (version 0.11.5)