##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660658_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28032408
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36060994117951	34.0	31.0	34.0	31.0	34.0
2	32.51556412849014	34.0	31.0	34.0	31.0	34.0
3	32.53899318959684	34.0	31.0	34.0	31.0	34.0
4	35.894267235265694	37.0	37.0	37.0	35.0	37.0
5	35.88877708971702	37.0	37.0	37.0	35.0	37.0
6	35.90312719478113	37.0	37.0	37.0	35.0	37.0
7	35.84945824133268	37.0	36.0	37.0	35.0	37.0
8	35.87393740844525	37.0	37.0	37.0	35.0	37.0
9	37.6011370482336	39.0	38.0	39.0	35.0	39.0
10-11	37.52432983994811	39.0	38.0	39.0	35.0	39.0
12-13	37.59152158815611	39.0	38.0	39.0	35.0	39.0
14-15	39.07308193074245	41.0	39.0	41.0	36.0	41.0
16-17	39.03187475011066	41.0	39.0	41.0	36.0	41.0
18-19	38.99502256459738	41.0	39.0	41.0	36.0	41.0
20-21	38.92377945198286	40.5	39.0	41.0	35.0	41.0
22-23	38.77784036605061	40.0	39.0	41.0	34.5	41.0
24-25	38.7765885827575	40.0	39.0	41.0	35.0	41.0
26-27	38.74963488687807	40.0	39.0	41.0	35.0	41.0
28-29	38.673576615323235	40.0	38.5	41.0	35.0	41.0
30-31	38.58108086897137	40.0	38.0	41.0	34.0	41.0
32-33	38.47424684315382	40.0	38.0	41.0	34.0	41.0
34-35	38.3683895974973	40.0	38.0	41.0	34.0	41.0
36-37	38.20693516589799	40.0	38.0	41.0	33.5	41.0
38-39	37.841258535477934	40.0	37.0	41.0	33.0	41.0
40-41	37.70123874124549	40.0	37.0	41.0	33.0	41.0
42-43	37.54878087890273	40.0	36.5	41.0	33.0	41.0
44-45	37.40628043798449	40.0	36.0	41.0	32.5	41.0
46-47	37.23677758970974	40.0	36.0	41.0	32.0	41.0
48-49	37.24858884759383	40.0	36.0	41.0	32.0	41.0
50-51	36.27572210350249	38.5	34.5	40.0	30.5	40.5
52-53	36.45091142366364	38.5	35.0	39.5	31.0	40.5
54-55	36.83771804405815	40.0	36.0	41.0	30.5	41.0
56-57	36.53643732639736	40.0	35.0	41.0	29.5	41.0
58-59	36.309375990817486	39.0	35.0	41.0	29.0	41.0
60-61	36.09134026231354	39.0	35.0	41.0	28.5	41.0
62-63	35.826417998054254	38.5	35.0	41.0	28.0	41.0
64-65	35.53696949616315	37.5	35.0	40.5	28.0	41.0
66-67	35.22977881529121	37.0	35.0	40.0	28.0	41.0
68-69	34.92267633590379	36.5	35.0	39.5	28.0	41.0
70-71	34.585067879291714	36.0	34.0	39.0	27.0	41.0
72-73	34.22892473240258	35.5	34.0	39.0	26.0	41.0
74-75	33.866723543692714	35.0	34.0	37.5	26.0	40.0
76-77	33.5037788048747	35.0	34.0	37.0	26.0	39.0
78-79	33.16006439760723	35.0	34.0	36.5	25.5	39.0
80-81	32.84546199170617	35.0	33.5	36.0	25.0	38.5
82-83	32.579560343157105	35.0	33.0	36.0	25.0	37.0
84-85	32.34568639269234	35.0	33.0	35.0	24.0	37.0
86-87	32.144088513551885	35.0	33.0	35.0	24.0	36.5
88-89	31.955636169393653	35.0	33.0	35.0	23.5	36.0
90-91	31.819172134623614	35.0	33.0	35.0	23.0	36.0
92-93	31.690109658078608	35.0	33.0	35.0	23.0	36.0
94-95	31.58325346149357	35.0	33.0	35.0	22.5	35.0
96-97	31.47980143197117	35.0	33.0	35.0	20.5	35.0
98-99	31.231070320466227	35.0	32.0	35.0	19.5	35.0
100	31.159393513393496	35.0	32.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4787367044204274
1101	2	0.47172354586617615
1101	3	0.4603551881556811
1101	4	0.4142986054280797
1101	5	0.3987918753927815
1101	6	0.3603401457501221
1101	7	0.39248654679351347
1101	8	0.3901430109919204
1101	9	0.523200136005201
1101	10-11	0.5011309287672248
1101	12-13	0.48956544303937477
1101	14-15	0.6168745345399458
1101	16-17	0.6324731056822657
1101	18-19	0.5910817125572905
1101	20-21	0.624519579282591
1101	22-23	0.6494874294474542
1101	24-25	0.6625200417147497
1101	26-27	0.6156612825077872
1101	28-29	0.6174786813763262
1101	30-31	0.6216200012161295
1101	32-33	0.6283691442323303
1101	34-35	0.6135008752187048
1101	36-37	0.6190301691221549
1101	38-39	0.6577703243015023
1101	40-41	0.666849624908096
1101	42-43	0.6589174310886321
1101	44-45	0.662678605524512
1101	46-47	0.6537418538099402
1101	48-49	0.639864678893943
1101	50-51	0.6284104177900574
1101	52-53	0.672087695845228
1101	54-55	0.731373181109106
1101	56-57	0.7340078697049464
1101	58-59	0.702942043712504
1101	60-61	0.6774024869194832
1101	62-63	0.6760550083085235
1101	64-65	0.6698744093797373
1101	66-67	0.6385907866773977
1101	68-69	0.5977810401906325
1101	70-71	0.579048679565318
1101	72-73	0.5247781899306432
1101	74-75	0.5401718055223839
1101	76-77	0.5034316780228281
1101	78-79	0.5020213555994815
1101	80-81	0.5153037535985092
1101	82-83	0.5006083147142775
1101	84-85	0.5279526285812608
1101	86-87	0.5456686100294803
1101	88-89	0.5230080901618948
1101	90-91	0.5346102931082726
1101	92-93	0.5353027666139667
1101	94-95	0.5238691354792735
1101	96-97	0.4873631802403082
1101	98-99	0.31158492739877985
1101	100	0.3509741861157991
1102	1	0.46001619883919886
1102	2	0.43929047310207636
1102	3	0.45310614227094703
1102	4	0.38566609768808036
1102	5	0.3925620055407606
1102	6	0.36213083043323024
1102	7	0.38598714175272164
1102	8	0.4030210602091131
1102	9	0.4711226372110602
1102	10-11	0.5074448461595935
1102	12-13	0.48222573239846866
1102	14-15	0.5618167845354307
1102	16-17	0.5985004393028603
1102	18-19	0.6045392177291191
1102	20-21	0.5638752665523086
1102	22-23	0.5911932399957252
1102	24-25	0.6075982694091664
1102	26-27	0.5833952686148152
1102	28-29	0.5742958540409688
1102	30-31	0.5749797098095328
1102	32-33	0.5917188059710767
1102	34-35	0.5958038367847962
1102	36-37	0.593314937033341
1102	38-39	0.6165996532856823
1102	40-41	0.6483184561920439
1102	42-43	0.6535414521270937
1102	44-45	0.6131376249800482
1102	46-47	0.6115578240602915
1102	48-49	0.6076263797707497
1102	50-51	0.6143794959343154
1102	52-53	0.6532952642273742
1102	54-55	0.6706316365294924
1102	56-57	0.6816116694708754
1102	58-59	0.6758571471697721
1102	60-61	0.6738051202370983
1102	62-63	0.6710513579560384
1102	64-65	0.6716988481033255
1102	66-67	0.6121132429373404
1102	68-69	0.5841836999198264
1102	70-71	0.53503810184813
1102	72-73	0.5435836432145251
1102	74-75	0.5586767641717785
1102	76-77	0.5425923704981344
1102	78-79	0.49486806652539883
1102	80-81	0.4642449592747866
1102	82-83	0.4611820048020405
1102	84-85	0.5172781020056121
1102	86-87	0.5329944046112871
1102	88-89	0.5292290954610621
1102	90-91	0.4907652787653092
1102	92-93	0.498086852287031
1102	94-95	0.4721668141032893
1102	96-97	0.49593952362191374
1102	98-99	0.32418176284425115
1102	100	0.32393716286382457
1103	1	0.41609158181760364
1103	2	0.4149339401923555
1103	3	0.40287954337109966
1103	4	0.3733564432463936
1103	5	0.38092289211153485
1103	6	0.34695532821995556
1103	7	0.3718922710087256
1103	8	0.3807365700802663
1103	9	0.45868808501673186
1103	10-11	0.4373302536725703
1103	12-13	0.40583222528258744
1103	14-15	0.5349141478957407
1103	16-17	0.5611526119281933
1103	18-19	0.554014483977511
1103	20-21	0.5499707249167187
1103	22-23	0.5606580761087869
1103	24-25	0.5666800197110788
1103	26-27	0.560003471069983
1103	28-29	0.5712028275104757
1103	30-31	0.5744815179753218
1103	32-33	0.5616524119595496
1103	34-35	0.551403969934789
1103	36-37	0.5245750554056485
1103	38-39	0.5888444191436051
1103	40-41	0.5960891340258243
1103	42-43	0.6228405162188793
1103	44-45	0.5856379905417413
1103	46-47	0.6020427388418668
1103	48-49	0.5278898851854166
1103	50-51	0.4798635396187976
1103	52-53	0.5758950772996556
1103	54-55	0.6653125204255161
1103	56-57	0.6561229944659388
1103	58-59	0.6002486854716196
1103	60-61	0.5881006169806327
1103	62-63	0.556398487645481
1103	64-65	0.5474215811041532
1103	66-67	0.5547579022193432
1103	68-69	0.5283405557276737
1103	70-71	0.5098194473164455
1103	72-73	0.4835060567642273
1103	74-75	0.4776932052958287
1103	76-77	0.46880354839219507
1103	78-79	0.49718197614556914
1103	80-81	0.49828687127804017
1103	82-83	0.4911166107950251
1103	84-85	0.4689762071024788
1103	86-87	0.4622255032398428
1103	88-89	0.46693023664163746
1103	90-91	0.4743306028901486
1103	92-93	0.4521002054279393
1103	94-95	0.48684530121116154
1103	96-97	0.46233939337307817
1103	98-99	0.3261732646602127
1103	100	0.3431686520654047
1104	1	0.37481810421115114
1104	2	0.36638957434760044
1104	3	0.3527415614734508
1104	4	0.33020663834642505
1104	5	0.3255005471448911
1104	6	0.312397634299046
1104	7	0.3294053690190637
1104	8	0.36212795809407794
1104	9	0.4252372888661782
1104	10-11	0.4108189242991429
1104	12-13	0.37753060334262756
1104	14-15	0.4942142451338114
1104	16-17	0.5015117039140122
1104	18-19	0.49680383204568557
1104	20-21	0.499855298490651
1104	22-23	0.5018891290327545
1104	24-25	0.5161569151337346
1104	26-27	0.49132852080240497
1104	28-29	0.5280411809879197
1104	30-31	0.5010553775946747
1104	32-33	0.4903588914170811
1104	34-35	0.5104977565934306
1104	36-37	0.5031807946885749
1104	38-39	0.5001888783392232
1104	40-41	0.4745489274100265
1104	42-43	0.5132992386020945
1104	44-45	0.48486175118576824
1104	46-47	0.46459282638588917
1104	48-49	0.45443246708433094
1104	50-51	0.35918170067023425
1104	52-53	0.4084315826105609
1104	54-55	0.475542306211878
1104	56-57	0.4299327902208745
1104	58-59	0.41930408535709063
1104	60-61	0.4123685143634077
1104	62-63	0.42421799525858006
1104	64-65	0.4149748861622058
1104	66-67	0.38785786840532666
1104	68-69	0.39452696321500724
1104	70-71	0.417804874898124
1104	72-73	0.40039376905200896
1104	74-75	0.3800252658926837
1104	76-77	0.37509772952203235
1104	78-79	0.37235046431852936
1104	80-81	0.39801943777013093
1104	82-83	0.38566611587999233
1104	84-85	0.40232223308753845
1104	86-87	0.3782976672636167
1104	88-89	0.37516609594463546
1104	90-91	0.3915873850804985
1104	92-93	0.39337473762266484
1104	94-95	0.3844692790403812
1104	96-97	0.39024785304744825
1104	98-99	0.22081546913410577
1104	100	0.28375565629373867
1105	1	0.33063772384554113
1105	2	0.3213139889620038
1105	3	0.3250483240849249
1105	4	0.3261572013130518
1105	5	0.3051374384219656
1105	6	0.27963830488570096
1105	7	0.2735238762889054
1105	8	0.2867753046000274
1105	9	0.3441041644725402
1105	10-11	0.34595632620467853
1105	12-13	0.3509375018988621
1105	14-15	0.4518957989816741
1105	16-17	0.43305854616339445
1105	18-19	0.44930271447553594
1105	20-21	0.4307856578023177
1105	22-23	0.45979680287538116
1105	24-25	0.455961844206378
1105	26-27	0.44943215166364325
1105	28-29	0.4476197678713305
1105	30-31	0.4662982260978623
1105	32-33	0.47328165860026417
1105	34-35	0.4516206243267149
1105	36-37	0.4892490586088769
1105	38-39	0.5052523707205339
1105	40-41	0.5075032980405183
1105	42-43	0.48551086295307044
1105	44-45	0.5158624083345487
1105	46-47	0.5286952294568366
1105	48-49	0.4956197727896239
1105	50-51	0.40772058407547007
1105	52-53	0.4612697129867769
1105	54-55	0.579992696772365
1105	56-57	0.5790492606818773
1105	58-59	0.5487368579992093
1105	60-61	0.48841277101051617
1105	62-63	0.4593358919361705
1105	64-65	0.5076235289183515
1105	66-67	0.5060915901686016
1105	68-69	0.4987778860528991
1105	70-71	0.48540158681213086
1105	72-73	0.47543151721899335
1105	74-75	0.4773032455515249
1105	76-77	0.44766432057568295
1105	78-79	0.4509145949241784
1105	80-81	0.4052773959070848
1105	82-83	0.4333486877766788
1105	84-85	0.45412559156461185
1105	86-87	0.4078907099032918
1105	88-89	0.4102907177182331
1105	90-91	0.4060635378710771
1105	92-93	0.41113639494169973
1105	94-95	0.42241829969935907
1105	96-97	0.4324139924869215
1105	98-99	0.3357902959721706
1105	100	0.32180321213341045
1106	1	0.36338385257590033
1106	2	0.3309947217299367
1106	3	0.32647890378166267
1106	4	0.31099024880931125
1106	5	0.30281036065223077
1106	6	0.29883585606587815
1106	7	0.30090373178119023
1106	8	0.31731165860023225
1106	9	0.380321429732291
1106	10-11	0.3851501303244973
1106	12-13	0.3744447377587008
1106	14-15	0.41191863846005106
1106	16-17	0.4599249855569454
1106	18-19	0.4523662846230394
1106	20-21	0.47101557614904976
1106	22-23	0.4752682335435239
1106	24-25	0.4553610759476143
1106	26-27	0.42782368685179506
1106	28-29	0.4613597550670221
1106	30-31	0.44473011143016805
1106	32-33	0.47264559269376605
1106	34-35	0.4733038042386184
1106	36-37	0.4959256558112273
1106	38-39	0.5053865819169516
1106	40-41	0.5044709822135758
1106	42-43	0.4944955033639573
1106	44-45	0.49034905707535614
1106	46-47	0.521028988026714
1106	48-49	0.5202690806591264
1106	50-51	0.4992386529265431
1106	52-53	0.49904389695456786
1106	54-55	0.5595217868155942
1106	56-57	0.5621215140075009
1106	58-59	0.5389881684062985
1106	60-61	0.5699986543720996
1106	62-63	0.5464323929884571
1106	64-65	0.5247481267718896
1106	66-67	0.5351159891875312
1106	68-69	0.546341605198343
1106	70-71	0.5409319489738706
1106	72-73	0.5442890332288712
1106	74-75	0.5427785549541682
1106	76-77	0.5234612600297197
1106	78-79	0.4985947492569096
1106	80-81	0.48916597238392256
1106	82-83	0.47984381307360735
1106	84-85	0.5166337139638415
1106	86-87	0.47052382464646314
1106	88-89	0.5137971568830153
1106	90-91	0.5002483588883422
1106	92-93	0.5021369262269104
1106	94-95	0.5564833793778377
1106	96-97	0.5293200657970942
1106	98-99	0.42398365259941073
1106	100	0.49038165211537077
1107	1	0.32181522461753787
1107	2	0.2951035998370699
1107	3	0.2740469116557662
1107	4	0.28725892411497966
1107	5	0.30888995452087187
1107	6	0.2704326057239186
1107	7	0.24938433807732707
1107	8	0.27265486325371313
1107	9	0.3566909487434273
1107	10-11	0.3596240529076695
1107	12-13	0.31272224432812123
1107	14-15	0.3655379223859896
1107	16-17	0.38387941052140917
1107	18-19	0.4111130290274474
1107	20-21	0.392708065700198
1107	22-23	0.394330960793404
1107	24-25	0.3973769139728276
1107	26-27	0.3772331018772732
1107	28-29	0.3733433124153862
1107	30-31	0.39221862376658834
1107	32-33	0.39678201844936467
1107	34-35	0.3932585869806573
1107	36-37	0.3906272720956494
1107	38-39	0.3977363723610239
1107	40-41	0.4279266635875061
1107	42-43	0.4275590161719407
1107	44-45	0.4793008354798829
1107	46-47	0.42143569275418
1107	48-49	0.4034346867383789
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2310	80-81	-9.041432197776089E-4
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2310	100	0.1724330350028893
2311	1	-0.07799621164924275
2311	2	-0.14432783607707478
2311	3	-0.13629603568230664
2311	4	-0.09290363203005825
2311	5	-0.09705326279390647
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2311	7	-0.10205326027700323
2311	8	-0.07335826967736381
2311	9	-0.12360586433403142
2311	10-11	-0.1032847400034953
2311	12-13	-0.08073056017263269
2311	14-15	-0.14151542252350424
2311	16-17	-0.15447486502689145
2311	18-19	-0.1458476663921715
2311	20-21	-0.14075402639452506
2311	22-23	-0.14426266502244545
2311	24-25	-0.15405778291660255
2311	26-27	-0.16594915313155667
2311	28-29	-0.1559024837543319
2311	30-31	-0.1491387975218501
2311	32-33	-0.17518648113580326
2311	34-35	-0.17224006851247253
2311	36-37	-0.13709442791670767
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2311	40-41	-0.11677562480124948
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2311	44-45	-0.15309489076405214
2311	46-47	-0.13545471237750917
2311	48-49	-0.11442407188803116
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2311	52-53	-0.22196405574061373
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2311	78-79	-0.10394017685416657
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2311	86-87	-0.12014687960161297
2311	88-89	-0.10557924761322113
2311	90-91	-0.11144753162571419
2311	92-93	-0.10761199906946928
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2311	96-97	-0.1235178313241363
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2312	4	-0.13886748529072435
2312	5	-0.15990601977389218
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2312	8	-0.1301906517340683
2312	9	-0.218987207266629
2312	10-11	-0.214135634533541
2312	12-13	-0.2248843069875619
2312	14-15	-0.23205197960218982
2312	16-17	-0.23451260145679242
2312	18-19	-0.24531087172482557
2312	20-21	-0.27816453007157094
2312	22-23	-0.23525948964709187
2312	24-25	-0.22204071518721236
2312	26-27	-0.21098142133202913
2312	28-29	-0.2300996356615599
2312	30-31	-0.23140594477232668
2312	32-33	-0.19607727561791677
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2312	38-39	-0.30290189357752695
2312	40-41	-0.3103042782432226
2312	42-43	-0.25688126905632913
2312	44-45	-0.26069286130910285
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2312	48-49	-0.23623399549333612
2312	50-51	-0.23244896260465708
2312	52-53	-0.3207685876565094
2312	54-55	-0.36606727980188936
2312	56-57	-0.29396235784975744
2312	58-59	-0.23228598137843193
2312	60-61	-0.2779810184336071
2312	62-63	-0.2516699305550958
2312	64-65	-0.25819600431609047
2312	66-67	-0.2233793608733592
2312	68-69	-0.18977432654244097
2312	70-71	-0.210903397089389
2312	72-73	-0.2059020888635743
2312	74-75	-0.14281300018447496
2312	76-77	-0.15452645105597895
2312	78-79	-0.17566139150049764
2312	80-81	-0.130663316948052
2312	82-83	-0.1358690928321593
2312	84-85	-0.17734571244784547
2312	86-87	-0.12647538346342913
2312	88-89	-0.1540422622371871
2312	90-91	-0.17077847937217427
2312	92-93	-0.11004571516835426
2312	94-95	-0.10525242452918704
2312	96-97	-0.10088493356354178
2312	98-99	0.00649114881356283
2312	100	-0.02672948469382419
2313	1	-0.3129629077362921
2313	2	-0.30997931497724807
2313	3	-0.2554579433676949
2313	4	-0.2248655592842539
2313	5	-0.264282448659948
2313	6	-0.20496403741788782
2313	7	-0.1988306317520454
2313	8	-0.18476136423544176
2313	9	-0.29187248796723253
2313	10-11	-0.23744397377057425
2313	12-13	-0.24332232813137722
2313	14-15	-0.28814648135261933
2313	16-17	-0.2976417806696574
2313	18-19	-0.3550913046271944
2313	20-21	-0.34191794771897577
2313	22-23	-0.3252443104231446
2313	24-25	-0.31532522803662744
2313	26-27	-0.32007170322456346
2313	28-29	-0.33322603313265375
2313	30-31	-0.33135496811706133
2313	32-33	-0.3096344611380104
2313	34-35	-0.32363296333439706
2313	36-37	-0.33894601419902415
2313	38-39	-0.2970628026086999
2313	40-41	-0.4043685122568803
2313	42-43	-0.3279204126685684
2313	44-45	-0.34853956836237643
2313	46-47	-0.3495685539042128
2313	48-49	-0.35899984357962467
2313	50-51	-0.3353371214408156
2313	52-53	-0.39687816793927766
2313	54-55	-0.3735476378118108
2313	56-57	-0.38056251608018243
2313	58-59	-0.40830932248167073
2313	60-61	-0.3999803108159128
2313	62-63	-0.31323858872278976
2313	64-65	-0.33354379145045954
2313	66-67	-0.29729293061855344
2313	68-69	-0.24730658626049973
2313	70-71	-0.3091668884875105
2313	72-73	-0.3095880060305731
2313	74-75	-0.29260754072299733
2313	76-77	-0.26097644888523774
2313	78-79	-0.24279235557423107
2313	80-81	-0.32397791976854506
2313	82-83	-0.2531839244668035
2313	84-85	-0.23349010889641875
2313	86-87	-0.2530592457209835
2313	88-89	-0.20579364246246
2313	90-91	-0.22792110746894068
2313	92-93	-0.25549304614386514
2313	94-95	-0.2327279463408729
2313	96-97	-0.22876612789767137
2313	98-99	-0.13372703172805345
2313	100	-0.11887301202247968
2314	1	-0.3433711260483534
2314	2	-0.355264577197822
2314	3	-0.3313965981922067
2314	4	-0.3133037404624446
2314	5	-0.3095670876616836
2314	6	-0.30172923249037353
2314	7	-0.32372412365219105
2314	8	-0.3380347464091926
2314	9	-0.4893118313239242
2314	10-11	-0.4418335159589972
2314	12-13	-0.39193565374676353
2314	14-15	-0.45130174610594764
2314	16-17	-0.44960035805549836
2314	18-19	-0.42920592254917267
2314	20-21	-0.4372559093247048
2314	22-23	-0.446636278428592
2314	24-25	-0.43256247648057666
2314	26-27	-0.4392409612351713
2314	28-29	-0.441954942160649
2314	30-31	-0.43222714418784847
2314	32-33	-0.48762473368906
2314	34-35	-0.4892818824734988
2314	36-37	-0.486480797288948
2314	38-39	-0.4266751829257558
2314	40-41	-0.41845272831738356
2314	42-43	-0.4386035178539558
2314	44-45	-0.40165267734690246
2314	46-47	-0.40579806870092483
2314	48-49	-0.4056806485889126
2314	50-51	-0.4322207592539442
2314	52-53	-0.49294825587795543
2314	54-55	-0.5428065520848193
2314	56-57	-0.5374350493800151
2314	58-59	-0.4667728586951725
2314	60-61	-0.45987173487595356
2314	62-63	-0.5025588551513422
2314	64-65	-0.4567249895861849
2314	66-67	-0.43512114258874846
2314	68-69	-0.4371424727967792
2314	70-71	-0.42634165924269496
2314	72-73	-0.39725788302213516
2314	74-75	-0.3823027231416489
2314	76-77	-0.32683114242111344
2314	78-79	-0.39002331947938984
2314	80-81	-0.3674483998863707
2314	82-83	-0.34348852142868935
2314	84-85	-0.3385280043412351
2314	86-87	-0.33954472646701106
2314	88-89	-0.33353580536089567
2314	90-91	-0.33915853465205004
2314	92-93	-0.37906814974776637
2314	94-95	-0.3159260723467483
2314	96-97	-0.29489428986077826
2314	98-99	-0.19687268644686284
2314	100	-0.19412568986919254
2315	1	-0.45054240399923984
2315	2	-0.4538544332429808
2315	3	-0.48255764663240797
2315	4	-0.3876286443083785
2315	5	-0.38639558511265193
2315	6	-0.3763059018811603
2315	7	-0.3819840679225379
2315	8	-0.37776598438612297
2315	9	-0.4854706046521997
2315	10-11	-0.4358652646256047
2315	12-13	-0.5105911877381999
2315	14-15	-0.7258654536993063
2315	16-17	-0.6691983942526818
2315	18-19	-0.6688093061604405
2315	20-21	-0.678295210572621
2315	22-23	-0.6410587432536659
2315	24-25	-0.6454541242302625
2315	26-27	-0.6529335478293561
2315	28-29	-0.6556701151275135
2315	30-31	-0.6548937291882027
2315	32-33	-0.5840191865769242
2315	34-35	-0.5724400747640672
2315	36-37	-0.5855964607132265
2315	38-39	-0.5707980735158813
2315	40-41	-0.6333032787725159
2315	42-43	-0.5802897218197884
2315	44-45	-0.5775579870731562
2315	46-47	-0.555923755323974
2315	48-49	-0.5246392917648919
2315	50-51	-0.5828678387114508
2315	52-53	-0.6207297140998804
2315	54-55	-0.6772480330166388
2315	56-57	-0.7077282364671476
2315	58-59	-0.6657602080730314
2315	60-61	-0.6144435023311132
2315	62-63	-0.5632207243670351
2315	64-65	-0.5938869385903374
2315	66-67	-0.5602636593969734
2315	68-69	-0.5622834612809768
2315	70-71	-0.4919221391516544
2315	72-73	-0.5089308698018229
2315	74-75	-0.4553946379600049
2315	76-77	-0.4409202974634141
2315	78-79	-0.502561093858219
2315	80-81	-0.4882075042491607
2315	82-83	-0.4914032218279729
2315	84-85	-0.4764702823023299
2315	86-87	-0.46608264178303216
2315	88-89	-0.4038074202765429
2315	90-91	-0.43951863809343905
2315	92-93	-0.41980313919291135
2315	94-95	-0.37864621767996454
2315	96-97	-0.3950588688194543
2315	98-99	-0.34498727379845917
2315	100	-0.3395633170700165
2316	1	-0.6732052884994921
2316	2	-0.6712238920033826
2316	3	-0.709685670336988
2316	4	-0.5835527565562231
2316	5	-0.519744891983045
2316	6	-0.49465087745682723
2316	7	-0.53827189010822
2316	8	-0.5182816189787118
2316	9	-0.6691363401172552
2316	10-11	-0.6924703280962632
2316	12-13	-0.5614060172648863
2316	14-15	-0.7398091175528876
2316	16-17	-0.7595430137684005
2316	18-19	-0.9197302062860757
2316	20-21	-0.8705739736376401
2316	22-23	-0.7526213646073359
2316	24-25	-0.7492142201161158
2316	26-27	-0.7415082688720389
2316	28-29	-0.7823777489145272
2316	30-31	-0.8037122289368384
2316	32-33	-0.7921466194665285
2316	34-35	-0.8205103275428627
2316	36-37	-0.778315652498506
2316	38-39	-0.753465657923968
2316	40-41	-0.8015166443210404
2316	42-43	-0.8275549690103432
2316	44-45	-0.7632518179862942
2316	46-47	-0.7232122684052698
2316	48-49	-0.6767658492709216
2316	50-51	-0.6430263484253445
2316	52-53	-0.7487248702116034
2316	54-55	-0.8601604063700563
2316	56-57	-0.7973248634322587
2316	58-59	-0.7519490775934159
2316	60-61	-0.7272815236812491
2316	62-63	-0.6722224573928557
2316	64-65	-0.6778305742355784
2316	66-67	-0.6308556525034774
2316	68-69	-0.619487456201739
2316	70-71	-0.7169624897702036
2316	72-73	-0.6798364466333666
2316	74-75	-0.6145894447999325
2316	76-77	-0.5814487097065282
2316	78-79	-0.5743881653672887
2316	80-81	-1.0939608591953416
2316	82-83	-0.9090894928526829
2316	84-85	-0.6691896010704745
2316	86-87	-0.5817505056985439
2316	88-89	-0.6347712742601068
2316	90-91	-0.6429235193763461
2316	92-93	-0.616639138050715
2316	94-95	-0.5603688114993908
2316	96-97	-0.6020528073287963
2316	98-99	-0.4428956093689571
2316	100	-0.48193937757872973
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	830.0
3	264.0
4	268.0
5	246.0
6	900.0
7	7632.0
8	15828.0
9	19705.0
10	21536.0
11	21090.0
12	21274.0
13	22416.0
14	27695.0
15	36250.0
16	48779.0
17	63761.0
18	70818.0
19	79088.0
20	90670.0
21	109517.0
22	143472.0
23	211695.0
24	319044.0
25	416014.0
26	427828.0
27	365104.0
28	319851.0
29	324028.0
30	370556.0
31	447086.0
32	559335.0
33	727836.0
34	1029798.0
35	1666544.0
36	3171983.0
37	6580421.0
38	8040102.0
39	2248416.0
40	4728.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	16.888444215320632	31.30773441476218	28.057707480965593	23.7461138889516
2	22.287067764888853	32.83344708011964	25.042124143249712	19.837361011741795
3	26.220362522511593	24.488235991968523	26.38037122833245	22.91103025718743
4	20.66363827779387	27.04594926332425	23.687252899482903	28.60315955939898
5	22.942084202148962	30.598410145048504	25.59064051814554	20.868865134656993
6	28.565150703860194	26.79143202101709	24.624164127867918	20.0192531472548
7	22.385468713479085	25.124273498666817	30.462952576509828	22.02730521134427
8	27.477869140516333	26.32199767514492	24.533980618213306	21.666152566125447
9	26.46353093613545	24.938048508848134	26.40152145718172	22.1968990978347
10-11	21.87822155706314	28.714577055541625	27.693027242803158	21.71417414459208
12-13	21.698502471975537	26.221891245920624	27.744438724013214	24.335167558090625
14-15	21.698554142319047	31.134799351678698	25.34637201782789	21.820274488174366
16-17	24.354705324278367	25.907118183854976	25.438657912593793	24.299518579272863
18-19	21.758507467718886	28.460145956858373	25.495436183411098	24.285910392011647
20-21	24.26455922186873	28.613250579445864	25.45487008916105	21.66732010952435
22-23	24.20975876502857	26.119401227704138	27.939968704405487	21.730871302861804
24-25	21.823986156515225	28.275576328711406	25.409889350239272	24.490548164534097
26-27	21.88391940396341	25.85447707117127	30.495355407530976	21.76624811733434
28-29	21.926911413239036	25.923677802814698	30.425721242658327	21.72368954128794
30-31	21.81855462557489	25.860418437247034	30.488755730561536	21.83227120661654
32-33	21.934851667004473	26.033698968661984	30.258070153162116	21.773379211171427
34-35	21.91816906496874	26.174639350289574	30.15609734639452	21.75109423834716
36-37	21.851429582989258	26.12449727057249	30.264899294287094	21.75917385215116
38-39	21.91710825176365	26.128536625530792	30.27747508640864	21.676880036296918
40-41	21.942400433037328	26.1985639938345	30.226019902197294	21.63301567093088
42-43	21.974793091382768	26.20662943966021	30.178674358988815	21.639903109968202
44-45	22.0322820759875	26.306567824119842	30.109835716887673	21.551314383004986
46-47	22.082223206820746	26.394741558765528	30.007472981494814	21.515562252918908
48-49	22.131485767164325	26.40551920596492	29.926604895386916	21.53639013148384
50-51	22.264452632193795	26.47575646239274	29.812304562343645	21.447486343069823
52-53	22.409520203971343	26.595946102238965	29.545298810211772	21.44923488357792
54-55	22.703897725611903	26.86083931075273	28.878732176214548	21.556530787420815
56-57	23.145304521703856	27.309703463684503	27.97543320856744	21.5695588060442
58-59	23.34682040468636	27.650672845132284	27.514979673052924	21.487527077128433
60-61	23.44829236167178	27.786987561047873	27.346849520867465	21.417870556412883
62-63	23.586777725357937	27.90746781452198	27.214048616849553	21.291705843270535
64-65	23.749569871112456	27.93998960232664	27.180716437448364	21.129724089112536
66-67	23.877587637824288	27.789700514542346	27.306297572491307	21.02641427514206
68-69	24.00608625483021	27.691578135512213	27.44510033649778	20.8572352731598
70-71	24.16793131034595	27.595635503700507	27.52980242296284	20.706630762990702
72-73	24.32458773596223	27.535066043738237	27.51878188034052	20.621564339959015
74-75	24.56143841394562	27.608275494263108	27.36037910722648	20.469906984564794
76-77	24.850065638523407	27.68795815879378	27.07339407419454	20.38858212848827
78-79	25.09596803464809	27.76153642495844	26.806930646253342	20.335564894140127
80-81	25.33609547380271	27.887697283088343	26.519412180576285	20.25679506253266
82-83	25.51299340879163	27.96095769879709	26.29251336178962	20.23353553062166
84-85	25.666403661443816	27.93090307561944	26.171836546158715	20.230856716778025
86-87	25.852703526992	27.907854926270044	26.073778760429533	20.165662786308424
88-89	26.018510393253784	27.862201888745574	26.006291369682955	20.112996348317687
90-91	26.13779267546467	27.79512683570004	25.967508217214096	20.099572271621188
92-93	26.23074617548145	27.844656122614403	25.916780177839303	20.007817524064837
94-95	26.291046430811747	27.87726806527023	25.862469529690024	19.969215974228007
96-97	26.33500895292406	27.90748874115491	25.815024764953957	19.942477540967065
98-99	26.426117440436787	28.018799742470357	25.687295154908284	19.867787662184565
100	26.428871043959788	28.127181197347813	25.581409390072334	19.86253836862006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44538.0
1	29947.0
2	12504.0
3	8902.5
4	8028.0
5	7935.0
6	8028.0
7	8324.0
8	8368.5
9	8453.5
10	9047.5
11	13806.0
12	120141.0
13	140912.0
14	58602.0
15	61079.5
16	68404.5
17	74962.0
18	80488.5
19	85966.0
20	92764.5
21	101957.5
22	114725.5
23	129773.0
24	146256.0
25	166686.5
26	190652.5
27	218081.0
28	249841.0
29	283353.0
30	316263.5
31	350328.0
32	388490.5
33	429426.0
34	468998.0
35	514067.5
36	567867.0
37	612445.0
38	652513.0
39	694284.5
40	728933.0
41	762277.5
42	798001.0
43	830589.5
44	865064.0
45	907610.5
46	949452.0
47	990002.0
48	1026575.0
49	1053487.5
50	1073647.0
51	1082271.0
52	1076957.0
53	1056043.0
54	1017310.5
55	967290.0
56	905919.0
57	829797.0
58	740070.5
59	640121.5
60	529500.0
61	421768.0
62	328392.5
63	246889.0
64	181221.5
65	131966.5
66	94818.5
67	67676.5
68	48421.5
69	35447.0
70	27044.5
71	21160.0
72	17005.5
73	12994.0
74	9895.0
75	8021.5
76	6146.0
77	4261.0
78	3048.0
79	2263.5
80	1629.0
81	1236.0
82	969.0
83	719.5
84	572.5
85	479.5
86	419.5
87	366.0
88	306.5
89	275.5
90	235.0
91	181.5
92	149.0
93	145.0
94	123.0
95	87.0
96	59.0
97	49.5
98	47.5
99	32.0
100	19.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13498661977237203
2	0.05006348366504939
3	0.01848931422516396
4	0.07503458140306749
5	0.0786732270734644
6	0.08005733934808597
7	0.0476626909825228
8	0.044937274029401966
9	0.03307956990352024
10-11	0.022702295143535296
12-13	0.015046870036994326
14-15	0.012144871749868938
16-17	0.017281783284546944
18-19	0.01642919866177747
20-21	0.02316604410152706
22-23	0.019477456235654104
24-25	0.01706239435442007
26-27	0.015446407600802615
28-29	0.017526143312411833
30-31	0.015291230064859215
32-33	0.012410635575794986
34-35	0.009815425060879537
36-37	0.007744607598462466
38-39	0.016711015336249385
40-41	0.025365284352311085
42-43	0.02580584586240326
44-45	0.024346820294567632
46-47	0.0265728152929281
48-49	0.02316069315201177
50-51	0.02328376499086343
52-53	0.019725383563195857
54-55	0.02474279055869906
56-57	0.03112112238092425
58-59	0.02382777819158454
60-61	0.02374037934950148
62-63	0.030917786299343247
64-65	0.03150282344634824
66-67	0.024341469345052342
68-69	0.02483910764997427
70-71	0.035751477361488176
72-73	0.040376481392536814
74-75	0.041482344292363325
76-77	0.04243838060576173
78-79	0.03680383076616179
80-81	0.03200581270078546
82-83	0.03487748894065754
84-85	0.038471543365093716
86-87	0.038261072684158993
88-89	0.03756009829765606
90-91	0.04050312053106533
92-93	0.03377876064018474
94-95	0.03364855420197937
96-97	0.03367709259939425
98-99	0.3127219752223926
100	0.148974001805339
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.8032408E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.99892717545351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7944606540002	40.18432960722493
2	10.504431078017838	10.714294311348047
3	3.7843174228017897	5.789883859628051
4	1.9649513971980812	4.0084165283604225
5	1.155372087814	2.9461368483488926
6	0.7495075768425082	2.2934449397305023
7	0.5199643212631228	1.8562335787752478
8	0.3820873377199124	1.5588835448832579
9	0.28974173706782386	1.3298865973570213
>10	1.66508431850746	15.380010127834174
>50	0.13727317737894043	4.804910627496112
>100	0.051968383037206335	3.8714989256094436
>500	3.519075550677449E-4	0.11708487627079202
>1k	4.259960508283686E-4	0.40252301284627656
>5k	3.478041406069285E-5	0.1066493224619579
>10k+	2.7824331233090018E-5	4.635813291824778
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1216660	4.340190824848154	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40372	0.14401902255418086	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32494	0.11591583569987994	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.004091692729358106	0.0	0.0	0.0	0.0
2	0.004098827328711825	0.0	0.0	3.567299676859726E-6	0.0
3	0.004102394628388685	3.567299676859726E-6	0.0	3.567299676859726E-6	3.567299676859726E-6
4	0.004109529227742405	1.4269198707438904E-5	0.0	1.0701899030579177E-5	7.134599353719452E-6
5	0.004113096527419264	2.8538397414877808E-5	0.0	1.0701899030579177E-5	1.0701899030579177E-5
6	0.004116663827096124	3.2105697091737536E-5	0.0	1.0701899030579177E-5	1.783649838429863E-5
7	0.004116663827096124	3.2105697091737536E-5	0.0	1.0701899030579177E-5	2.4971097738018083E-5
8	0.004116663827096124	3.9240296445456985E-5	0.0	1.0701899030579177E-5	2.8538397414877808E-5
9	0.004116663827096124	3.9240296445456985E-5	0.0	1.0701899030579177E-5	3.2105697091737536E-5
10-11	0.004123798426449843	4.81585456376063E-5	0.0	1.0701899030579177E-5	3.567299676859726E-5
12-13	0.004130933025803562	5.350949515289589E-5	0.0	1.0701899030579177E-5	4.280759612231671E-5
14-15	0.0041452022245110015	6.064409450661534E-5	0.0	1.248554886900904E-5	5.172584531446603E-5
16-17	0.004189793470471749	6.24277443450452E-5	0.0	1.4269198707438904E-5	7.848059289091397E-5
18-19	0.0042201155177250555	7.491329321405424E-5	0.0	1.6052848545868768E-5	8.739884208306328E-5
20-21	0.004259355814170513	9.810074111364246E-5	0.0	2.318744789958822E-5	9.096614175992302E-5
22-23	0.004512634091227553	1.0701899030579178E-4	0.0	2.8538397414877808E-5	9.453344143678274E-5
24-25	0.005650602688145806	1.0701899030579178E-4	0.0	2.8538397414877808E-5	9.810074111364246E-5
26-27	0.010138265681635342	1.1236993982108137E-4	0.0	2.8538397414877808E-5	1.0345169062893205E-4
28-29	0.0234371588769684	1.248554886900904E-4	0.0	3.38893469301674E-5	1.159372394979411E-4
30-31	0.054331757728412054	1.3555738772066958E-4	0.0	3.745664660702712E-5	1.2663913852852028E-4
32-33	0.11429271434690877	1.3912468739752932E-4	0.0	4.637489579917644E-5	1.3734103755909947E-4
34-35	0.21457842651262782	1.4982658642810848E-4	0.0	5.7076794829755615E-5	1.4625928675124876E-4
36-37	0.3607788528192084	1.5696118578182794E-4	0.0	5.7076794829755615E-5	1.5874483562025783E-4
38-39	0.5566146868296151	1.6587943497397726E-4	0.0	6.064409450661534E-5	1.6944673465083698E-4
40-41	0.8056175552239393	1.7123038448926684E-4	0.0	6.064409450661534E-5	1.730140343276967E-4
42-43	1.1086329080255966	1.837159333582759E-4	0.0	6.24277443450452E-5	1.7658133400455644E-4
44-45	1.4639644942382404	1.8728323303513562E-4	0.0	6.777869386033479E-5	1.926341825504252E-4
46-47	1.8741326110835717	1.9620148222728492E-4	0.0	6.956234369876466E-5	1.9620148222728495E-4
48-49	2.3375979687510258	2.0511973141943424E-4	0.0	7.669694305248411E-5	2.0868703109629399E-4
50-51	2.851758222126333	2.122543307731537E-4	0.0	8.20478925677737E-5	2.1403798061158356E-4
52-53	3.4121328428153586	2.21172579965303E-4	0.0	8.20478925677737E-5	2.2652352948059263E-4
54-55	4.01456592669456	2.2473987964216274E-4	0.0	8.20478925677737E-5	2.3722542851117178E-4
56-57	4.6584867058156405	2.3722542851117178E-4	0.0	8.20478925677737E-5	2.461436777033211E-4
58-59	5.347209201578401	2.568455767339003E-4	0.0	8.20478925677737E-5	2.4971097738018085E-4
60-61	6.071030358861786	2.6754747576447944E-4	0.0	8.20478925677737E-5	2.514946272186107E-4
62-63	6.824017758303175	2.72898425279769E-4	0.0	8.20478925677737E-5	2.639801760876197E-4
64-65	7.604154091935306	2.889512738256378E-4	1.783649838429863E-6	8.561519224463342E-5	2.657638259260496E-4
66-67	8.409261523305453	3.0678777220993645E-4	3.567299676859726E-6	8.561519224463342E-5	2.6754747576447944E-4
68-69	9.241039870709645	3.246242705942351E-4	3.567299676859726E-6	8.561519224463342E-5	2.764657249566288E-4
70-71	10.092811149152794	3.3889346930167396E-4	3.567299676859726E-6	8.739884208306328E-5	2.800330246334885E-4
72-73	10.962666853307786	3.424607689785337E-4	3.567299676859726E-6	9.63170912752126E-5	2.889512738256378E-4
74-75	11.850797833707329	3.602972673628323E-4	3.567299676859726E-6	1.0345169062893205E-4	2.9073492366406766E-4
76-77	12.752730696556643	3.7099916639341153E-4	3.567299676859726E-6	1.0345169062893205E-4	3.050041223715066E-4
78-79	13.668110495537878	3.7813376574713097E-4	3.567299676859726E-6	1.0701899030579178E-4	3.174896712405156E-4
80-81	14.595183902859862	3.8705201493928026E-4	3.567299676859726E-6	1.1058628998265151E-4	3.1927332107894546E-4
82-83	15.530378267896214	3.959702641314296E-4	3.567299676859726E-6	1.1236993982108137E-4	3.406771191401038E-4
84-85	16.475154399864614	4.1559041235415806E-4	3.567299676859726E-6	1.1772088933637096E-4	3.442444188169636E-4
86-87	17.42747715429941	4.387778602537463E-4	3.567299676859726E-6	1.1772088933637096E-4	3.478117184938233E-4
88	18.143560838583685	4.4591245960746577E-4	3.567299676859726E-6	1.1772088933637096E-4	3.4959536833225317E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	161725	0.0	82.86735	8
GTGGTAT	162765	0.0	82.398735	6
AGTGGTA	164235	0.0	81.79401	5
TGGTATC	163950	0.0	81.56666	7
GTATCAA	164415	0.0	81.30034	9
AAGCAGT	167660	0.0	81.09038	1
GCAGTGG	173115	0.0	78.42658	3
CAGTGGT	174310	0.0	77.333206	4
AGCAGTG	178110	0.0	76.50796	2
CAACGCA	159510	0.0	44.01575	12-13
ATCAACG	161640	0.0	43.464867	10-11
ACGCAGA	163505	0.0	42.953438	14-15
AGAGTAC	163860	0.0	42.302372	18-19
TCAACGC	159375	0.0	41.987843	12-13
AACGCAG	161855	0.0	41.19657	14-15
AGTACTT	163620	0.0	41.162174	20-21
GAGTACT	155545	0.0	40.982018	20-21
CGCAGAG	165200	0.0	40.252453	16-17
TATCAAC	166035	0.0	40.238525	10-11
GTACTTT	160730	0.0	39.544796	22-23
>>END_MODULE