FastQCFastQC Report
Thu 26 May 2016
SRR1660658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28032408
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11785054.204080505677572No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA391080.13950995576263017No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT334430.11930120309321983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1548700.082.150278
GTGGTAT1566600.081.322576
AGTGGTA1579150.080.869645
GTATCAA1574100.080.636659
TGGTATC1577650.080.565387
AAGCAGT1611650.080.0472261
GCAGTGG1659300.077.4474953
CAGTGGT1681250.076.193254
AGCAGTG1712400.075.45722
CAACGCA1523450.043.79584512-13
ATCAACG1540550.043.35690310-11
ACGCAGA1564850.042.6913114-15
AGAGTAC1576550.041.98140718-19
TCAACGC1519650.041.6316512-13
AGTACTT1570700.040.86448320-21
AACGCAG1547050.040.7986614-15
GAGTACT1493950.040.55792220-21
TATCAAC1591550.039.8910310-11
CGCAGAG1583050.039.80059416-17
GTACTTT1542850.039.13759222-23
Produced by FastQC (version 0.11.5)