##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1660658_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28032408
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77641036046565	34.0	31.0	34.0	31.0	34.0
2	32.95889828658316	34.0	31.0	34.0	31.0	34.0
3	33.01405148640816	34.0	31.0	34.0	31.0	34.0
4	36.392720382779814	37.0	37.0	37.0	35.0	37.0
5	36.31715787669757	37.0	37.0	37.0	35.0	37.0
6	36.30939090213013	37.0	37.0	37.0	35.0	37.0
7	36.2460544595384	37.0	37.0	37.0	35.0	37.0
8	36.27275972866833	37.0	37.0	37.0	35.0	37.0
9	38.05311134883596	39.0	38.0	39.0	35.0	39.0
10-11	37.979475024050735	39.0	38.0	39.0	35.0	39.0
12-13	38.039148313623286	39.0	38.0	39.0	35.0	39.0
14-15	39.55321888508472	41.0	40.0	41.0	37.0	41.0
16-17	39.527480746570184	41.0	39.5	41.0	37.0	41.0
18-19	39.490162314989135	41.0	39.0	41.0	37.0	41.0
20-21	39.445146417674856	41.0	39.0	41.0	36.5	41.0
22-23	39.30402755624847	40.5	39.0	41.0	36.0	41.0
24-25	39.30914318884057	41.0	39.0	41.0	36.0	41.0
26-27	39.27026789849805	41.0	39.0	41.0	36.0	41.0
28-29	39.20082063588686	40.5	39.0	41.0	36.0	41.0
30-31	39.115493556600626	40.0	39.0	41.0	35.5	41.0
32-33	39.02278591264796	40.0	39.0	41.0	35.0	41.0
34-35	38.921872444921604	40.0	38.0	41.0	35.0	41.0
36-37	38.770882472886385	40.0	38.0	41.0	35.0	41.0
38-39	38.44460397408599	40.0	38.0	41.0	35.0	41.0
40-41	38.32427490710038	40.0	38.0	41.0	34.5	41.0
42-43	38.19205867009356	40.0	37.5	41.0	34.0	41.0
44-45	38.06326953788629	40.0	37.0	41.0	34.0	41.0
46-47	37.92447140110118	40.0	37.0	41.0	33.5	41.0
48-49	37.76380245321772	40.0	36.5	41.0	33.0	41.0
50-51	37.796752333941484	40.0	37.0	41.0	33.0	41.0
52-53	37.676066215931215	40.0	36.5	41.0	33.0	41.0
54-55	37.1839298108104	40.0	36.0	41.0	32.5	41.0
56-57	36.84046167207612	39.5	36.0	41.0	31.0	41.0
58-59	36.60890826788766	39.0	35.0	41.0	31.0	41.0
60-61	36.38894639375968	39.0	35.0	41.0	31.0	41.0
62-63	36.13343916084554	38.5	35.0	40.5	30.5	41.0
64-65	35.85933445318005	37.5	35.0	40.0	30.0	41.0
66-67	35.57808021701168	37.0	35.0	40.0	30.0	41.0
68-69	35.27802648991125	37.0	35.0	39.5	30.0	41.0
70-71	34.95434965843819	36.0	35.0	39.0	29.0	41.0
72-73	34.60040450324496	35.5	34.0	39.0	29.0	40.5
74-75	34.24790094736064	35.0	34.0	37.5	29.0	40.0
76-77	32.3926377819558	34.5	32.0	35.5	26.0	38.5
78-79	33.53013028705918	35.0	34.0	36.5	28.5	39.0
80-81	33.49667586530561	35.0	34.0	36.0	29.0	38.5
82-83	33.27220504924158	35.0	34.0	36.0	29.0	37.0
84-85	33.04624684757727	35.0	34.0	35.5	28.5	37.0
86-87	32.85672566908986	35.0	34.0	35.0	27.5	36.5
88-89	32.692075668276516	35.0	34.0	35.0	27.0	36.0
90-91	32.57584034521758	35.0	34.0	35.0	27.0	36.0
92-93	32.474766438188254	35.0	34.0	35.0	27.0	36.0
94-95	32.36712078034823	35.0	34.0	35.0	26.5	35.0
96-97	32.28335157650388	35.0	34.0	35.0	26.0	35.0
98-99	32.177227728706	35.0	33.0	35.0	25.0	35.0
100	32.11840449097345	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.37321510056246154
1101	2	0.33363645895098415
1101	3	0.33477810115061146
1101	4	0.22759905213022336
1101	5	0.23555228149927387
1101	6	0.2543006282614826
1101	7	0.2512219539067573
1101	8	0.24065750887711346
1101	9	0.3249014426642063
1101	10-11	0.3317723004598818
1101	12-13	0.3198900705651653
1101	14-15	0.42414310075165673
1101	16-17	0.4374876344646452
1101	18-19	0.42599135257201226
1101	20-21	0.44024988313609015
1101	22-23	0.4257935608327088
1101	24-25	0.45484654690479687
1101	26-27	0.47307404976753986
1101	28-29	0.46137719471231975
1101	30-31	0.47160906030955374
1101	32-33	0.4771940628351601
1101	34-35	0.48640885955283863
1101	36-37	0.5312260689878912
1101	38-39	0.5355479130402969
1101	40-41	0.5223283193993993
1101	42-43	0.5482284038362621
1101	44-45	0.5367427084912535
1101	46-47	0.5292840928647635
1101	48-49	0.5608733565909461
1101	50-51	0.5348119406691012
1101	52-53	0.5210137557765009
1101	54-55	0.38646506317191864
1101	56-57	0.4180715354614435
1101	58-59	0.41428134296111807
1101	60-61	0.37465768221332496
1101	62-63	0.30463980084931563
1101	64-65	0.3312228504203034
1101	66-67	0.29699041896125067
1101	68-69	0.3020991677173299
1101	70-71	0.3418310328628209
1101	72-73	0.38202494436522016
1101	74-75	0.38254897742145033
1101	76-77	0.3791462110368897
1101	78-79	0.4202478856793235
1101	80-81	0.4078818336830352
1101	82-83	0.38067088032469343
1101	84-85	0.38349541400637577
1101	86-87	0.417103909823183
1101	88-89	0.41644072410525723
1101	90-91	0.36764991930571256
1101	92-93	0.32151864011584763
1101	94-95	0.29784546799790235
1101	96-97	0.2723313959267557
1101	98-99	0.2380235424814927
1101	100	0.2560244896184827
1102	1	0.3114423382335332
1102	2	0.2549687242799408
1102	3	0.27524459688368097
1102	4	0.20734567653233427
1102	5	0.22654911184163495
1102	6	0.22836585783935703
1102	7	0.23875616470289174
1102	8	0.22644814416585746
1102	9	0.24369669670004868
1102	10-11	0.29793906922098046
1102	12-13	0.28379920465545183
1102	14-15	0.3748141360478172
1102	16-17	0.3759052912414802
1102	18-19	0.38622277844868336
1102	20-21	0.3799849857471571
1102	22-23	0.40003154280827147
1102	24-25	0.3833794119971614
1102	26-27	0.41311816479885977
1102	28-29	0.42250402021359434
1102	30-31	0.40947906243379606
1102	32-33	0.42208663103198063
1102	34-35	0.42763050962394544
1102	36-37	0.4249388763913515
1102	38-39	0.46089580648794737
1102	40-41	0.4549841978813802
1102	42-43	0.4222435088992853
1102	44-45	0.4389056258343018
1102	46-47	0.43625905487694183
1102	48-49	0.4313963382654009
1102	50-51	0.4245902941352213
1102	52-53	0.43103777515498365
1102	54-55	0.34159209519088307
1102	56-57	0.34830976621731935
1102	58-59	0.3293315106121568
1102	60-61	0.2570083546318074
1102	62-63	0.3237782466725818
1102	64-65	0.3264551939817508
1102	66-67	0.28641580835525104
1102	68-69	0.3067002204804723
1102	70-71	0.3096916022476819
1102	72-73	0.3270661088997855
1102	74-75	0.3362324958758549
1102	76-77	0.3417347139458897
1102	78-79	0.39183662760392224
1102	80-81	0.36934238412847975
1102	82-83	0.38128374662147024
1102	84-85	0.43388088999729746
1102	86-87	0.39720379064558387
1102	88-89	0.4042526012153189
1102	90-91	0.36766588491162366
1102	92-93	0.2955226985507764
1102	94-95	0.35445076271041387
1102	96-97	0.3632042911051201
1102	98-99	0.3754448419307934
1102	100	0.36732157335183757
1103	1	0.27991931565311035
1103	2	0.2507430901967709
1103	3	0.2341524960191066
1103	4	0.16836930789373383
1103	5	0.1770026248065122
1103	6	0.18665377698025054
1103	7	0.20251387077178862
1103	8	0.1671495026496359
1103	9	0.24367064229794266
1103	10-11	0.261415168851336
1103	12-13	0.2463007158032937
1103	14-15	0.3259182856433469
1103	16-17	0.3152368635554481
1103	18-19	0.32173266459322036
1103	20-21	0.3249527834183823
1103	22-23	0.34364972600240407
1103	24-25	0.33219936086996427
1103	26-27	0.2983860265215199
1103	28-29	0.34360838294751517
1103	30-31	0.3539209866368864
1103	32-33	0.33998755661265534
1103	34-35	0.3488161030970929
1103	36-37	0.3372021951968307
1103	38-39	0.37980016395939487
1103	40-41	0.3415442218626481
1103	42-43	0.36214803972279697
1103	44-45	0.34723193717788803
1103	46-47	0.36790566557345983
1103	48-49	0.34589377718758385
1103	50-51	0.36576815862176915
1103	52-53	0.376218481294849
1103	54-55	0.28991216611449033
1103	56-57	0.28311816964393444
1103	58-59	0.2969517546874343
1103	60-61	0.2485285374730637
1103	62-63	0.24872913305912192
1103	64-65	0.24630900990596416
1103	66-67	0.2276571077775955
1103	68-69	0.2409583737399572
1103	70-71	0.23952291855630392
1103	72-73	0.2618427574723583
1103	74-75	0.25999593765182283
1103	76-77	0.21554538787774646
1103	78-79	0.3727360057871394
1103	80-81	0.34611778208227406
1103	82-83	0.34554996309967123
1103	84-85	0.34225621924583294
1103	86-87	0.3435479030416033
1103	88-89	0.3317226209692592
1103	90-91	0.29724425312857505
1103	92-93	0.277457657902616
1103	94-95	0.2639524392022139
1103	96-97	0.32169512539900325
1103	98-99	0.3007639117133394
1103	100	0.3152170311719047
1104	1	0.25176480817726343
1104	2	0.2062560817644794
1104	3	0.19586972997347374
1104	4	0.1588445641574836
1104	5	0.1587217390233846
1104	6	0.16983840896805447
1104	7	0.18240703558088
1104	8	0.16861155311669052
1104	9	0.22278569132777903
1104	10-11	0.23266083580127628
1104	12-13	0.1953378155228549
1104	14-15	0.2408606664914288
1104	16-17	0.2642897461063214
1104	18-19	0.2731720120183212
1104	20-21	0.2873916124995475
1104	22-23	0.28768021288961165
1104	24-25	0.29430537077056584
1104	26-27	0.263179460873296
1104	28-29	0.2755781352442881
1104	30-31	0.2708201266303547
1104	32-33	0.30940866587538807
1104	34-35	0.2806085189973331
1104	36-37	0.28137098431737684
1104	38-39	0.28398484460943507
1104	40-41	0.30630747027479543
1104	42-43	0.2838776379148271
1104	44-45	0.32308065398271424
1104	46-47	0.2944839103124224
1104	48-49	0.287020379081973
1104	50-51	0.2575308152167253
1104	52-53	0.25720351569146516
1104	54-55	0.19172471901186583
1104	56-57	0.2012823236630581
1104	58-59	0.18291863288922627
1104	60-61	0.13411206263988618
1104	62-63	0.1279736348989715
1104	64-65	0.14611725720376967
1104	66-67	0.13650437033038543
1104	68-69	0.11178091444011073
1104	70-71	0.11996625570839115
1104	72-73	0.12660633461850068
1104	74-75	0.12462719936267774
1104	76-77	0.12823588083285387
1104	78-79	0.24132969396955417
1104	80-81	0.22995685147998302
1104	82-83	0.2368592667134024
1104	84-85	0.24015088073043245
1104	86-87	0.273931543778815
1104	88-89	0.28409905825670023
1104	90-91	0.260448864465161
1104	92-93	0.2448644675722491
1104	94-95	0.2679664193234501
1104	96-97	0.3058074471363952
1104	98-99	0.29856189780726794
1104	100	0.2749791256557188
1105	1	0.19525737344179106
1105	2	0.1386398488192242
1105	3	0.15223316974401513
1105	4	0.1297332678562313
1105	5	0.09872463766540562
1105	6	0.12662290564870204
1105	7	0.1300372550822928
1105	8	0.14313967554652862
1105	9	0.16303212362523567
1105	10-11	0.1762131351843763
1105	12-13	0.16388924848981645
1105	14-15	0.21555862465872622
1105	16-17	0.20081109865358115
1105	18-19	0.23651978045825928
1105	20-21	0.232444381074842
1105	22-23	0.231296377374953
1105	24-25	0.23518237939223496
1105	26-27	0.2148228361827833
1105	28-29	0.24393674498781337
1105	30-31	0.24500947679327822
1105	32-33	0.2546509421409482
1105	34-35	0.2531570745303142
1105	36-37	0.2420054226530155
1105	38-39	0.223753775335922
1105	40-41	0.2485843545276154
1105	42-43	0.2516704269525576
1105	44-45	0.26808428139409557
1105	46-47	0.2517594559463845
1105	48-49	0.26141194911288324
1105	50-51	0.25274615238014064
1105	52-53	0.24913490416169992
1105	54-55	0.14974998760995817
1105	56-57	0.15284892020046215
1105	58-59	0.13865839131707247
1105	60-61	0.16084543500000592
1105	62-63	0.08274702602898998
1105	64-65	0.12158477227186637
1105	66-67	0.15978757405581945
1105	68-69	0.12459644419522675
1105	70-71	0.14515438574562012
1105	72-73	0.17280125301237348
1105	74-75	0.17603566013873007
1105	76-77	0.1451268906761527
1105	78-79	0.2646478949018345
1105	80-81	0.27338958148479264
1105	82-83	0.2665002273412611
1105	84-85	0.25545929556153624
1105	86-87	0.2550146617776434
1105	88-89	0.2711206801949828
1105	90-91	0.24626749307435603
1105	92-93	0.23464971362977138
1105	94-95	0.24256934827305088
1105	96-97	0.25279552861639587
1105	98-99	0.2560425158055821
1105	100	0.28424880488199733
1106	1	0.2344361564016424
1106	2	0.22657578748727047
1106	3	0.1949870552783537
1106	4	0.13452853085903627
1106	5	0.14291329917846696
1106	6	0.1599974325943876
1106	7	0.16606900610697295
1106	8	0.17403317570523313
1106	9	0.20821835641540787
1106	10-11	0.2114097861922346
1106	12-13	0.1943250945536974
1106	14-15	0.2592232663145282
1106	16-17	0.2502383194626745
1106	18-19	0.26836239514493343
1106	20-21	0.2383872340983686
1106	22-23	0.2519274659323969
1106	24-25	0.28298774552582273
1106	26-27	0.2530159766782205
1106	28-29	0.2975708631810434
1106	30-31	0.2695229258770482
1106	32-33	0.30664814293489684
1106	34-35	0.27843081044026263
1106	36-37	0.3027790192078399
1106	38-39	0.33505279826724177
1106	40-41	0.3295252374266724
1106	42-43	0.3114180392115742
1106	44-45	0.31153254519406204
1106	46-47	0.30039961951226957
1106	48-49	0.26758029576647857
1106	50-51	0.27577895899251814
1106	52-53	0.2611319430230523
1106	54-55	0.1681310294330558
1106	56-57	0.17882673703122265
1106	58-59	0.19169163817330315
1106	60-61	0.13270080711545518
1106	62-63	0.10475140106860437
1106	64-65	0.1419093759642891
1106	66-67	0.1504657048629099
1106	68-69	0.16676916462488833
1106	70-71	0.1499709526935149
1106	72-73	0.1759104585577731
1106	74-75	0.22220164330798298
1106	76-77	0.1576250693501109
1106	78-79	0.2893885949334418
1106	80-81	0.3022959625666459
1106	82-83	0.3042292264628301
1106	84-85	0.32231379184443654
1106	86-87	0.32936908607202753
1106	88-89	0.3253463228562339
1106	90-91	0.3115377914911832
1106	92-93	0.28668148221100864
1106	94-95	0.30684975474181186
1106	96-97	0.3065157021788707
1106	98-99	0.35635668997496595
1106	100	0.3778793475902731
1107	1	0.16535812963151386
1107	2	0.15061915293637895
1107	3	0.1361321008589016
1107	4	0.10471280470230226
1107	5	0.12634429158362792
1107	6	0.1112360791654936
1107	7	0.12401118164937941
1107	8	0.13239185502245476
1107	9	0.172362542417261
1107	10-11	0.17478517257875126
1107	12-13	0.14041750821841248
1107	14-15	0.18822493269734508
1107	16-17	0.16327979599048348
1107	18-19	0.20016071917417122
1107	20-21	0.1746208391651578
1107	22-23	0.19957581543983594
1107	24-25	0.17412604106767304
1107	26-27	0.1908113534345688
1107	28-29	0.23466475003957044
1107	30-31	0.20242372444833734
1107	32-33	0.19986041297648427
1107	34-35	0.2202930778499379
1107	36-37	0.22908453287096364
1107	38-39	0.17398284886750304
1107	40-41	0.2005707106583543
1107	42-43	0.16737694783603985
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2314	96-97	-0.320381785360798
2314	98-99	-0.36143880996294797
2314	100	-0.2935025775701945
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2315	4	-0.28328190704645095
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2315	28-29	-0.4602810251750995
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2315	96-97	-0.3836643389484138
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2315	100	-0.3949581603266026
2316	1	-1.619305253074348
2316	2	-0.8791872812374208
2316	3	-0.6200281513989907
2316	4	-0.3883770599310097
2316	5	-0.35123143818749725
2316	6	-0.3393730276499838
2316	7	-0.3321860685718363
2316	8	-0.33814479686808596
2316	9	-0.4413450463723052
2316	10-11	-0.4351717781329896
2316	12-13	-0.407955554854027
2316	14-15	-0.5500981159069838
2316	16-17	-0.5637939128800085
2316	18-19	-0.49835797334634435
2316	20-21	-0.5273073446729484
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2316	24-25	-0.5722326299536391
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2316	28-29	-0.597033602006583
2316	30-31	-0.6272137357546015
2316	32-33	-0.6074068501909764
2316	34-35	-0.5771637646392875
2316	36-37	-0.5647101845124851
2316	38-39	-0.6398324099122377
2316	40-41	-0.6404436374076425
2316	42-43	-0.6715561005555486
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2316	46-47	-0.5883643134456378
2316	48-49	-0.598720501437711
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2316	52-53	-0.574873785876882
2316	54-55	-0.5128612721314383
2316	56-57	-0.5279131233881174
2316	58-59	-0.48529403285778727
2316	60-61	-0.4131800335699012
2316	62-63	-0.3870945974241451
2316	64-65	-0.37697835726030604
2316	66-67	-0.43168313134652436
2316	68-69	-0.4377566847034515
2316	70-71	-0.43272296879876393
2316	72-73	-0.43502197515411467
2316	74-75	-0.44196856436320786
2316	76-77	-0.5063456293908501
2316	78-79	-0.6715141069804744
2316	80-81	-0.6103923534371916
2316	82-83	-0.6174445016102581
2316	84-85	-0.6076325582086994
2316	86-87	-0.6540535363878135
2316	88-89	-0.728949798983983
2316	90-91	-0.6314309389763508
2316	92-93	-0.5353744586721874
2316	94-95	-0.5246465285003907
2316	96-97	-0.5794610034859389
2316	98-99	-1.3400627044080693
2316	100	-0.8864981720188219
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	10.0
7	281.0
8	457.0
9	181.0
10	49.0
11	52.0
12	82.0
13	336.0
14	1391.0
15	4386.0
16	10264.0
17	18676.0
18	26179.0
19	34353.0
20	44767.0
21	58416.0
22	87451.0
23	146773.0
24	267912.0
25	382781.0
26	395180.0
27	330531.0
28	291443.0
29	307081.0
30	353700.0
31	430343.0
32	530985.0
33	682962.0
34	959471.0
35	1589182.0
36	3167371.0
37	7066274.0
38	8761946.0
39	2078691.0
40	2451.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	16.899945051379834	31.074792796349097	28.08074829543038	23.944513856840686
2	22.28007221834805	32.764091799010686	25.30019044172871	19.655645540912555
3	25.899751459096915	24.74793817213277	26.522116829920567	22.83019353884975
4	20.73510060213165	27.30287387369647	23.860786415494523	28.101239108677355
5	22.947825245694197	30.527163417427428	25.66369967217943	20.861311664698945
6	28.292542688448314	26.958422551498252	24.726152673006187	20.02288208704725
7	22.35465108812629	25.335294063927723	30.297333001146388	22.012721846799604
8	27.181871068657394	26.492543915599402	24.626389570243127	21.69919544550008
9	26.151813286964142	25.07457796704443	26.551061899498606	22.222546846492815
10-11	21.830375756517242	28.760499276408936	27.65422613711958	21.754898829954243
12-13	21.665243670825568	26.379269665310233	27.677973651068434	24.27751301279576
14-15	21.67821437245063	31.07099111856534	25.398963228560316	21.851831280423713
16-17	24.223609331028573	26.056029506990626	25.472984696855157	24.24737646512565
18-19	21.72714551211374	28.515507411132273	25.54382356296022	24.213523513793763
20-21	24.139721611863784	28.65364362286001	25.49412813262995	21.712506632646253
22-23	24.073874964255364	26.260901044571067	27.883213561444535	21.782010429729038
24-25	21.82163152317874	28.312985225940206	25.444475888472862	24.420907362408187
26-27	21.871527833458693	26.01099206241401	30.309579392261632	21.80790071186566
28-29	21.912846577996422	26.08547831947338	30.23289112372252	21.768783978807672
30-31	21.816507163078956	26.01127819481812	30.298108878657466	21.874105763445456
32-33	21.93147841811625	26.181039676668462	30.070967089182744	21.81651481603255
34-35	21.917998668159047	26.30265475587304	29.971512331587157	21.807834244380757
36-37	21.858350255485913	26.265197738911674	30.070860763227554	21.805591242374863
38-39	21.904532920744842	26.277181503318058	30.093531697100666	21.724753878836427
40-41	21.9285950570447	26.35987483135166	30.03992257567449	21.671607535929148
42-43	21.94148718143319	26.388136976843835	29.977533001899126	21.69284283982385
44-45	22.002884867184083	26.477058420165957	29.915075518159394	21.604981194490566
46-47	22.032219757191875	26.598324246370957	29.772824572857942	21.59663142357923
48-49	22.031473321792173	26.653519839105467	29.664792468021307	21.650214371081056
50-51	22.11826585927166	26.75575035596809	29.522543028100916	21.603440756659335
52-53	22.202450975669716	26.89809140599271	29.233793578124047	21.665664040213528
54-55	22.406200040594733	27.166858000923284	28.598365964795434	21.82857599368655
56-57	22.741714926988784	27.62588283330708	27.69122188089967	21.94118035880447
58-59	22.830099817226007	27.979759951298362	27.21804154723128	21.972098684244358
60-61	22.780838635118275	28.128042993896145	27.06447914447474	22.02663922651084
62-63	22.735517982657484	28.248466167689905	26.92955543121277	22.08646041843984
64-65	22.730139287159908	28.303039369530325	26.846634009211485	22.120187334098283
66-67	22.639796953361444	28.175061408098284	26.958154700369768	22.226986938170505
68-69	22.568067707492894	28.0977634564608	27.037199208313282	22.296969627733027
70-71	22.503514668415857	28.00786249233026	27.095288996954377	22.393333842299505
72-73	22.416492429576405	27.93059185086054	27.082365622086275	22.57055009747678
74-75	22.444363696072912	27.974929200570784	26.902605653771193	22.678101449585107
76-77	22.446562480399695	28.013369913669827	26.715928793785785	22.824138812144692
78-79	22.480001671072408	28.090290139946784	26.438427572951596	22.991280616029215
80-81	22.50504886327866	28.17631467683538	26.180013427722553	23.13862303216341
82-83	22.473049029445427	28.22510140241525	25.94214309580932	23.35970647233
84-85	22.46036847394759	28.153393276845907	25.807720566883436	23.57851768232307
86-87	22.4766707308381	28.092629575519425	25.70921563298798	23.721484060654493
88-89	22.45915472568445	27.996818116324434	25.644878679492233	23.899148478498883
90-91	22.442902034895518	27.840757932025667	25.609109025860604	24.107231007218207
92-93	22.444633722478244	27.740522397741117	25.580309095892844	24.23453478388779
94-95	22.447541575790765	27.611560905539555	25.5722443688811	24.36865314978857
96-97	22.427849037173214	27.488512898412544	25.582058749591045	24.50157931482319
98-99	22.429130392315574	27.43653015659903	25.57373055497269	24.560608896112704
100	22.353826553663588	27.44193307337018	25.537472084057317	24.66676828890892
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44685.0
1	29948.5
2	12134.0
3	8389.5
4	7646.5
5	7416.0
6	7393.0
7	7741.0
8	7763.0
9	7947.5
10	8591.0
11	13137.0
12	116587.5
13	136341.5
14	55518.5
15	57357.5
16	64294.5
17	71123.0
18	77460.0
19	83997.5
20	92264.5
21	103299.5
22	117426.0
23	133366.0
24	150642.0
25	171435.5
26	195502.0
27	223473.5
28	255795.5
29	289943.0
30	322030.0
31	354724.5
32	392812.0
33	432138.0
34	468736.0
35	511329.0
36	564061.5
37	607799.0
38	646255.0
39	686973.0
40	720975.0
41	753831.0
42	790468.5
43	825216.5
44	859567.0
45	898187.5
46	933008.5
47	962438.0
48	988769.5
49	1012780.5
50	1038053.0
51	1061203.0
52	1075989.0
53	1076351.5
54	1052891.0
55	1008974.0
56	949566.0
57	869887.5
58	772540.5
59	664362.0
60	546163.0
61	431131.0
62	332359.0
63	247229.5
64	178878.5
65	128863.5
66	91484.0
67	64485.0
68	45431.0
69	32505.0
70	24204.5
71	18689.0
72	14734.5
73	10980.5
74	8149.0
75	6333.5
76	4739.0
77	3031.5
78	1892.0
79	1197.0
80	696.0
81	418.0
82	243.5
83	130.0
84	83.0
85	61.0
86	39.5
87	21.5
88	14.0
89	11.5
90	7.0
91	3.5
92	2.5
93	0.5
94	0.5
95	0.5
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10660875084295292
2	0.07380743031422773
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.050041223715066E-4
20-21	0.002176052802884433
22-23	8.276135250314564E-4
24-25	8.383154240620357E-4
26-27	0.0068081914332867866
28-29	0.006724359890880585
30-31	0.007894434184890574
32-33	0.007962212878750909
34-35	0.0072558875427326825
36-37	0.010965879206666798
38-39	0.013680594260757049
40-41	0.01706061070458164
42-43	0.023667249706125854
44-45	0.012086011305200752
46-47	0.01875329440125158
48-49	0.01643454961129276
50-51	0.018967332381863163
52-53	0.02146800945534183
54-55	0.11142995635622885
56-57	0.1043256790497627
58-59	0.10139692601506085
60-61	0.14391021991403663
62-63	0.09451382128856001
64-65	0.07323487871609174
66-67	0.08303425092842542
68-69	0.07608336750806424
70-71	0.05289948690815288
72-73	0.03143147745281105
74-75	0.02033004085842358
76-77	0.012506952667070201
78-79	0.008930734741018324
80-81	0.005684492035075974
82-83	5.761188978128457E-4
84-85	0.0024400329789720527
86-87	0.0016195540532943156
88-89	0.0020868703109629396
90-91	0.0031053343687063918
92-93	0.004756994119092445
94-95	0.002104706809347238
96-97	0.0011236993982108139
98-99	0.001548208059757121
100	0.0017372749426306866
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.8032408E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.199204977647774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.17035092512683	39.240894692651
2	10.706039956691116	10.748693885696492
3	3.96952829412639	5.978014935042694
4	2.008668361758015	4.033342192960262
5	1.1915326254384349	2.99069952509694
6	0.7965612026963995	2.399204345483892
7	0.5239158976267587	1.8410113075209793
8	0.40276749546647916	1.6174886450604486
9	0.3014834784527016	1.362080783899928
>10	1.7319902177229907	15.754583326275801
>50	0.14135167111757993	4.88183149653618
>100	0.05493900779317925	4.043841098081702
>500	3.8765762122362443E-4	0.12858281010853378
>1k	4.1950698755979847E-4	0.3588351238534028
>5k	3.5389652457340064E-5	0.10842532651211803
>10k+	2.8311721944521946E-5	4.512470505219565
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1178505	4.204080505677572	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39108	0.13950995576263017	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	33443	0.11930120309321983	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.004120231126772984	3.567299676859726E-6	0.0	0.0	3.567299676859726E-6
2	0.004130933025803563	1.4269198707438904E-5	0.0	0.0	7.134599353719452E-6
3	0.004134500325480422	1.4269198707438904E-5	0.0	7.134599353719452E-6	7.134599353719452E-6
4	0.004134500325480422	1.4269198707438904E-5	0.0	1.0701899030579177E-5	7.134599353719452E-6
5	0.004134500325480422	1.783649838429863E-5	0.0	1.0701899030579177E-5	1.0701899030579177E-5
6	0.004141634924834142	2.1403798061158355E-5	0.0	1.4269198707438904E-5	1.0701899030579177E-5
7	0.0041452022245110015	2.8538397414877808E-5	0.0	1.4269198707438904E-5	1.0701899030579177E-5
8	0.004148769524187862	2.8538397414877808E-5	0.0	1.4269198707438904E-5	1.0701899030579177E-5
9	0.004148769524187862	3.567299676859726E-5	0.0	1.783649838429863E-5	1.0701899030579177E-5
10-11	0.0041630387228953	4.102394628388685E-5	0.0	2.318744789958822E-5	1.6052848545868768E-5
12-13	0.00417195697208745	5.529314499132575E-5	0.0	2.8538397414877808E-5	1.9620148222728493E-5
14-15	0.004179091571441169	6.77786938603348E-5	0.0	3.2105697091737536E-5	2.6754747576447945E-5
16-17	0.004207629968856047	7.848059289091397E-5	0.0	3.2105697091737536E-5	3.032204725330767E-5
18-19	0.004243302965624644	8.561519224463342E-5	0.0	3.2105697091737536E-5	3.567299676859726E-5
20-21	0.004284326911908531	9.453344143678274E-5	0.0	3.9240296445456985E-5	4.4591245960746576E-5
22-23	0.004532254239450282	1.0345169062893206E-4	0.0	4.102394628388685E-5	5.529314499132575E-5
24-25	0.005702328533460272	1.159372394979411E-4	0.0	4.4591245960746576E-5	6.24277443450452E-5
26-27	0.010181073277757658	1.2485548869009043E-4	0.0	4.81585456376063E-5	6.421139418347507E-5
28-29	0.02356558166533535	1.3377373788223975E-4	0.0	5.350949515289589E-5	6.421139418347507E-5
30-31	0.05474734814076622	1.3912468739752932E-4	0.0	6.064409450661534E-5	7.134599353719452E-5
32-33	0.11488131879359062	1.444756369128189E-4	0.0	6.421139418347507E-5	8.561519224463342E-5
34-35	0.21552911187651091	1.4625928675124876E-4	0.0	6.421139418347507E-5	8.561519224463342E-5
36-37	0.3620416769048167	1.4804293658967862E-4	0.0	7.312964337562438E-5	9.096614175992302E-5
38-39	0.5583626636712765	1.5517753594339808E-4	0.0	7.848059289091397E-5	9.810074111364246E-5
40-41	0.8077079928345792	1.6231213529711754E-4	0.0	8.20478925677737E-5	1.0523534046736192E-4
42-43	1.110584220948839	1.783649838429863E-4	0.0	8.561519224463342E-5	1.1772088933637096E-4
44-45	1.464966905447438	1.8549958319670577E-4	0.0	8.918249192149315E-5	1.2663913852852028E-4
46-47	1.8737865830149163	1.979851320657148E-4	0.0	8.918249192149315E-5	1.3020643820538E-4
48-49	2.336130024933998	2.0511973141943424E-4	0.0	8.918249192149315E-5	1.3555738772066958E-4
50-51	2.8484459843763688	2.122543307731537E-4	0.0	9.63170912752126E-5	1.444756369128189E-4
52-53	3.4066552541615405	2.2652352948059263E-4	0.0	9.63170912752126E-5	1.551775359433981E-4
54-55	4.007210154760875	2.3900907834960164E-4	0.0	9.63170912752126E-5	1.6766308481240712E-4
56-57	4.64878543434442	2.47927327541751E-4	0.0	9.988439095207233E-5	1.7123038448926684E-4
58-59	5.335556617183939	2.711147754413392E-4	0.0	1.0166804079050219E-4	1.8549958319670577E-4
60-61	6.057565586231479	2.925185735024975E-4	0.0	1.0523534046736192E-4	1.926341825504252E-4
62-63	6.8089155951211895	3.0678777220993645E-4	0.0	1.0701899030579178E-4	1.926341825504252E-4
64-65	7.588183291282005	3.1035507188679616E-4	0.0	1.1058628998265151E-4	2.0333608158100438E-4
66-67	8.392377493934877	3.139223715636559E-4	0.0	1.1058628998265151E-4	2.1403798061158356E-4
68-69	9.224330639023233	3.174896712405156E-4	0.0	1.1058628998265151E-4	2.2295622980373288E-4
70-71	10.076683387313711	3.2997522010952467E-4	0.0	1.1415358965951123E-4	2.283071793190225E-4
72-73	10.947136614164577	3.442444188169636E-4	0.0	1.159372394979411E-4	2.407927281880315E-4
74-75	11.836623168441328	3.567299676859726E-4	0.0	1.2663913852852026E-4	2.4257637802646136E-4
76-77	12.74090866542753	3.7278281623184134E-4	0.0	1.3377373788223972E-4	2.47927327541751E-4
78-79	13.65794190780899	3.888356647777101E-4	0.0	1.3555738772066958E-4	2.4971097738018085E-4
80-81	14.587105752741612	4.0667216316200876E-4	0.0	1.3734103755909947E-4	2.568455767339003E-4
82-83	15.524404824587313	4.1737406219258797E-4	0.0	1.3912468739752932E-4	2.7111477544133916E-4
84-85	16.472099007691384	4.209413618694477E-4	0.0	1.4804293658967862E-4	2.764657249566288E-4
86-87	17.428083595244473	4.2272501170787755E-4	0.0	1.5161023626653836E-4	2.889512738256378E-4
88	18.14861213492612	4.280759612231671E-4	0.0	1.5339388610496822E-4	3.0322047253307673E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	154870	0.0	82.15027	8
GTGGTAT	156660	0.0	81.32257	6
AGTGGTA	157915	0.0	80.86964	5
GTATCAA	157410	0.0	80.63665	9
TGGTATC	157765	0.0	80.56538	7
AAGCAGT	161165	0.0	80.047226	1
GCAGTGG	165930	0.0	77.447495	3
CAGTGGT	168125	0.0	76.19325	4
AGCAGTG	171240	0.0	75.4572	2
CAACGCA	152345	0.0	43.795845	12-13
ATCAACG	154055	0.0	43.356903	10-11
ACGCAGA	156485	0.0	42.69131	14-15
AGAGTAC	157655	0.0	41.981407	18-19
TCAACGC	151965	0.0	41.63165	12-13
AGTACTT	157070	0.0	40.864483	20-21
AACGCAG	154705	0.0	40.79866	14-15
GAGTACT	149395	0.0	40.557922	20-21
TATCAAC	159155	0.0	39.89103	10-11
CGCAGAG	158305	0.0	39.800594	16-17
GTACTTT	154285	0.0	39.137592	22-23
>>END_MODULE