FastQCFastQC Report
Thu 26 May 2016
SRR1660657_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660657_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23433946
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10617224.530700890067767No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA306580.13082730497031955No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT282470.12053881151727497No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1417100.082.602288
GTGGTAT1425900.082.125916
AGTGGTA1435300.081.7361455
TGGTATC1434650.081.440647
GTATCAA1434700.081.414569
AAGCAGT1471100.080.700511
CAGTGGT1502600.078.330434
GCAGTGG1517000.078.039463
AGCAGTG1564500.076.031442
CAACGCA1395900.043.80702612-13
ATCAACG1411700.043.45241510-11
ACGCAGA1422950.042.93404414-15
AGAGTAC1421650.042.40357618-19
TCAACGC1395650.041.8033212-13
AGTACTT1402050.041.59484520-21
GAGTACT1340300.041.1341320-21
AACGCAG1414600.041.04495614-15
CGCAGAG1436300.040.24498716-17
TATCAAC1449350.040.24061610-11
GTACTTT1381350.039.7549522-23
Produced by FastQC (version 0.11.5)